Human alpha-thrombin
Biotinylated Thrombin

Thrombin Cleavage Kit

$300 – $1,200

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SKU: BIOTHROMKT Categories: , ,

Product Description

Thrombin Cleavage Kit

Each kit contains 10 units of biotinylated thrombin for cleavage of up to 10 mg recombinant protein along with 2 ml of 10X thrombin cleavage buffer and 10 ug of cleavage control protein. Cleavage control protein is converted from a single 67 kDa band to two bands of 39 kDa and 28 kDa following thrombin cleavage. Store all components at -20 C.

Biotinylated thrombin is useful for cleavage of proteins and peptides at the enzyme recognition sequence Leu-Val-Pro-Arg-||-Gly-Ser. Biotinylated thrombin is then easily removed from the reaction with immobilized avidin agarose resin. This preparation is >95% pure on SDS-PAGE and is tested for activity using thrombin chromogenic substrate and cleavage control protein.

Gene ID: 2147
Swiss-Prot/UniProt ID: P00734

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Additional Information

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Extinction Coefficient

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Gene Name

Gene ID

UniProt ID

1. Middendorp SJ, Wilbs J, Quarroz C, Calzavarini S, Angelillo-scherrer A, Heinis C. Peptide Macrocycle Inhibitor of Coagulation Factor XII with Subnanomolar Affinity and High Target Selectivity. J Med Chem. 2017;60(3):1151-1158. Link to article

2. Swedberg JE, Li CY, De veer SJ, Wang CK, Craik DJ. Design of Potent and Selective Cathepsin G Inhibitors Based on the Sunflower Trypsin Inhibitor-1 Scaffold. J Med Chem. 2017;60(2):658-667. Link to article

3. De veer SJ, Li CY, Swedberg JE, Schroeder CI, Craik DJ. Engineering potent mesotrypsin inhibitors based on the plant-derived cyclic peptide, sunflower trypsin inhibitor-1. Eur J Med Chem. 2018;155:695-704. Link to article

4. Lira AL. Allosteric inhibition of a-thrombin enzymaticactivity with ultrasmall gold nanoparticles. Nanoscale Advances. September 2018. Link to article

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