Thrombin Cleavage Kit

Name: Thrombin Cleavage Kit
SKU: BIOTHROMKT
Availability: InStock

$300 – $1,200

SKU: BIOTHROMKT Categories: Human Proteins, Human Thrombin, Thrombin

Product Description

Thrombin Cleavage Kit

Each kit contains 10 units of biotinylated thrombin for cleavage of up to 10 mg recombinant protein along with 2 ml of 10X thrombin cleavage buffer and 10 ug of cleavage control protein. Cleavage control protein is converted from a single 67 kDa band to two bands of 39 kDa and 28 kDa following thrombin cleavage. Store all components at -20 C.

Biotinylated thrombin is useful for cleavage of proteins and peptides at the enzyme recognition sequence Leu-Val-Pro-Arg-||-Gly-Ser. Biotinylated thrombin is then easily removed from the reaction with immobilized avidin agarose resin. This preparation is >95% pure on SDS-PAGE and is tested for activity using thrombin chromogenic substrate and cleavage control protein.

Gene ID: 2147
Swiss-Prot/UniProt ID: P00734

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Additional Information

Purity	>95% by SDS-PAGE analysis
Source	Human plasma
Molecular Weight	36,700
Extinction Coefficient	1.83
Species	Human
Storage	-20 C
Shipping Conditions	Cold pack
Expiration	12 months from delivery
Form	Liquid
Buffer	0.05M Na Citrate; 0.2M NaCl; 0.1% PEG-8000; 50% Glycerol; pH 6.5
Sample Size	10 unit kit
Sample Volume	0.02ml biotinylated thrombin at 0.5mg/ml, 0.01ml cleavage control protein at 1mg/ml, 2ml 10X buffer
Activity	0.5 U/ul (1 U is defined as the amount of enzyme required to cleave 1 mg of cleavage control protein when incubated in standard digest buffer at room temperature overnight)
Gene Name	F2
Gene ID	2147
UniProt ID	P00734

1. Middendorp SJ, Wilbs J, Quarroz C, Calzavarini S, Angelillo-scherrer A, Heinis C. Peptide Macrocycle Inhibitor of Coagulation Factor XII with Subnanomolar Affinity and High Target Selectivity. J Med Chem. 2017;60(3):1151-1158. Link to article

2. Swedberg JE, Li CY, De veer SJ, Wang CK, Craik DJ. Design of Potent and Selective Cathepsin G Inhibitors Based on the Sunflower Trypsin Inhibitor-1 Scaffold. J Med Chem. 2017;60(2):658-667. Link to article

3. De veer SJ, Li CY, Swedberg JE, Schroeder CI, Craik DJ. Engineering potent mesotrypsin inhibitors based on the plant-derived cyclic peptide, sunflower trypsin inhibitor-1. Eur J Med Chem. 2018;155:695-704. Link to article

4. Lira AL. Allosteric inhibition of a-thrombin enzymaticactivity with ultrasmall gold nanoparticles. Nanoscale Advances. September 2018. Link to article

Thrombin Cleavage Kit

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Thrombin Cleavage Kit

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Thrombin Cleavage Kit

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