Thrombin Cleavage Kit
Each kit contains 10 units of biotinylated thrombin for cleavage of up to 10 mg recombinant protein along with 2 ml of 10X thrombin cleavage buffer and 10 ug of cleavage control protein. Cleavage control protein is converted from a single 67 kDa band to two bands of 39 kDa and 28 kDa following thrombin cleavage. Store all components at -20 C.
Biotinylated thrombin is useful for cleavage of proteins and peptides at the enzyme recognition sequence Leu-Val-Pro-Arg-||-Gly-Ser. Biotinylated thrombin is then easily removed from the reaction with immobilized avidin agarose resin. This preparation is >95% pure on SDS-PAGE and is tested for activity using thrombin chromogenic substrate and cleavage control protein.
Gene ID: 2147
Swiss-Prot/UniProt ID: P00734
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