Soluble Human Fibrin
Normal human plasma clots are dissociated using non-denaturing conditions in which non fibrin bound plasma proteins are removed. The soluble human fibrin clot is then stored under mildly acidic conditions. The soluble human fibrin will reassemble into an insoluble clot when pH is restored to neutral (e.g. by adding one-tenth volume of 10X PBS to the soluble fibrin). Reassembled fibrin clots trap high molecular weight compounds such as dextran blue. SDS-PAGE shows partially crosslinked fibrin with no observable free alpha, beta or gamma chain subunits. Abundant plasma proteins such as albumin, IgG, and a1PI are also not observed. No thrombin or plasmin activity is detectable in the final product.