Soluble Dog Fibrin
Dog (canine) fibrinogen is allowed to clot then is dissociated using non-denaturing conditions. The soluble fibrin clot is then stored under acidic conditions. The soluble fibrin will reassemble into an insoluble clot when pH is restored to neutral (e.g. by adding one-tenth volume of 10X PBS to the soluble fibrin). SDS-PAGE shows partially crosslinked fibrin with no observable free alpha, beta or gamma chain subunits. No detectable thrombin or plasmin activity is observed in the final product.