Recombinant human Factor beta-XIIa
The low molecular weight beta form of human Factor XIIa is generated in vivo by kallikrein cleavage of Factor alpha-XIIa and contains the catalytic light chain and the C-terminal peptide of the heavy chain of Factor alpha-XIIa. Recombinant Human Factor beta-XIIa is activated from insect cell culture derived Factor XII by an extended autoactivation process with Dextran Sulfate followed by repurification. Complete activation is observed on SDS-PAGE. Factor XIIa activates Factor XI to XIa thereby triggering the Contact Factor cascade. The protein purity is determined by SDS-PAGE and activity is determined via complementation in an APTT clotting assay using Factor XII immuno-deficient human plasma. Specific activity of this recombinant product is typically lower than that of plasma derived material. Custom mutants of Factor XII are also available, please contact us for more details.