RealCount One Step RT-qPCR Kit with UNG
The RealCount One Step RT-qPCR Kit with UNG is ideal for accurate quantification of RNA or DNA in a variety of samples such as whole blood, serum, plasma, urine, saliva, cerebral spinal fluid, feces, and plant. The kit can be used for intercalating dye and probe-based chemistries on a variety of qPCR instruments. Aptamer modified warm start Reality Reverse Transcriptase synthesizes complementary DNA (cDNA) from single-stranded RNA or DNA templates. PCR amplification of the cDNA by TaqTivate Hot Start Taq is monitored in real time. The 4X master mix formulation contains inhibitor reducing buffer components optimized for mammalian tissue samples. Uracil-DNA Glycosylase (UNG) and dUTP are included in the master mix to reduce potential false positive results from carry over contamination. The kit contains Reality Reverse Transcriptase (200U/µl), 4X TaqTivate RT-qPCR Master Mix, DTT solution (100mM), 20X TealReveal Dye, and ROX passive fluorescent reference dye (25µM).