Mouse plasminogen total antigen assay ELISA kit


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Mouse plasminogen total antigen assay ELISA kit

Plasminogen is a single chain glycoprotein zymogen and is the precursor of the fibrinolytic enzyme plasmin. Plasminogen deficiencies are classified as hypoplasminogenemia (Type I) or dysplasminogenemia (Type 2) and are associated with decreased extracellular fibrin clearance leading to mucous membrane lesions and ligneous conjunctivitis. The sensitive quantitative measurement of total mouse plasminogen antigen in plasma, serum, or cell culture media samples is easily performed with this 96 well strip format ELISA kit. Plasminogen, plasmin and plasmin antiplasmin complex will be detected by the assay. Plasminogen antigen was found to be 84 ug/ml in a small sample of normal mice. The assay measures total mouse plasminogen in the 2.5-500 ng/ml range. Samples giving mouse plasminogen levels above 500 ng/ml should be diluted in a similar fluid depleted of plasminogen or blocking buffer before use. A 1:1,000-1:10,000 dilution for plasma is suggested for best results. Mouse plasminogen will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, anti-mouse plasminogen primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of plasminogen in the samples. A standard calibration curve is prepared using dilutions of purified plasminogen and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Gene ID: 18815
Swiss-Prot/UniProt ID: P20918

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1. Mignemi NA, Yuasa M, Baker CE, et al. Plasmin Prevents Dystrophic Calcification after Muscle Injury. J Bone Miner Res. 2016; Link to article

2. Amaro E, Mignemi NA, et al. Abstract P20: Severe Injury Leads to Plasmin Consumption Below a Critical Threshold Required to Heal Soft Tissue Injury. Plastic and Reconstructive Surgery. April 2017 – Volume 5 – Issue 4S – p 115–116. Link to article

3. Zheng Z, Nayak L, Wang W, et al. An ATF6-tPA pathway in hepatocytes contributes to systemic fibrinolysis and is repressed by DACH1. Blood. 2019;133(7):743-753. Link to article