Immobilized anhydrotrypsin selectively adsorbs peptides with Arg, Lys or AECys (S-aminoethyl cysteine) residues at the C-terminus under weak acidic conditions (1). Strongly acidic conditions are used to elute the bound peptides. Peptides with these amino acids at the N-terminus or internally, as well as free amino acids are not bound. Serine protease inhibitors such as ecotin and PAI-1 may also be purified on this resin (2). May be used repeatedly.
1. Kumazaki, T. et al. (1987) J. Biochem. (Tokyo) 102:1539-1546.
2. Blouse, G. E. et al. (2003) Biochemistry 42:12260-12272.