DH5-alpha Competent Cells
DH5α strain chemically competent cells from Molecular Innovations are an all purpose reagent for general cloning, sub-cloning and plasmid isolation. For a transformation efficiency of at least 1×106 simply thaw a tube of cells on ice, add 1-5 ul of DNA, gently mix and incubate on ice for 2-5 minutes, then spread 50-100 ul onto a pre-warmed culture plate. For greater transformation efficiencies heat shock at 37C for 1 minute before plating, or heat shock at 42C for 30 seconds then add SOC media and shake at 225 rpm at 37C for 1 hour before plating. Conveniently supplied as a pack of 5 single use 0.2 ml aliquots.
Genotype: F– Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rk–, mk+) phoA supE44 thi-1 gyrA96 relA1 λ–
Other strains of competent cells available upon request Click here to request a strain.