BL21(DE3) Competent Cells
BL21(DE3) strain chemically competent cells from Molecular Innovations are an all purpose reagent for T7 promoter driven IPTG inducible E. coli protein expression. Simply mix 1 ng DNA with 0.1 ml BL21 cells, keep on ice for 5 minutes, plate cells on LB plate with antibiotic. For greater transformation efficiencies heat shock at 37C for 1 minute before plating, or heat shock at 42C for 30 seconds then add SOC media and shake at 225 rpm at 37C for 1 hour before plating. Conveniently supplied as a pack of 10 single use 0.1 ml aliquots.
Genotype: E. coli str. B F– ompT gal dcm lon hsdS20(rB– mB–) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS)
Other strains of competent cells available upon request Click here to request a strain.