BL21(DE3) Competent Cells
BL21(DE3) strain chemically competent cells from Molecular Innovations are an all purpose reagent for T7 promoter driven IPTG inducible E. coli protein expression. For a transformation efficiency of at least 1×106 simply thaw a tube of cells on ice, add 1-5 ul of DNA, gently mix and incubate on ice for 2-5 minutes, then spread 50-100 ul onto a pre-warmed culture plate. For greater transformation efficiencies heat shock at 37C for 1 minute before plating, or heat shock at 42C for 30 seconds then add SOC media and shake at 225 rpm at 37C for 1 hour before plating. Conveniently supplied as a pack of 5 single use 0.2 ml aliquots.
Genotype: E. coli str. B F– ompT gal dcm lon hsdS20(rB– mB–) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS)
Other strains of competent cells available upon request Click here to request a strain.