Product Datasheet

Product Description
Product: TMB Substrate for ELISA
Catalog Number: TMB
Product Page:
Description: This one-component water based tetramethylbenzidine (TMB) chromogenic substrate is ideal for color development of horseradish peroxide (HRP) based ELISA systems. It is identical to the TMB substrate supplied in our ELISA kits. For best results, add 100 ul to each well and shake at 300 rpm at room temperature for 2-15 minutes. Quench reaction with 50 ul of 1N (0.5M) H2SO4, mix gently, and read plate at 450 nm. Store at 4C and bring to room temperature before use.
Recommended Storage: 4 C
Shipping Conditions: Cold pack
Minimum Shelf Life: 12 months from delivery
Source: Chemical
Sample Characteristics
Lot Number: TMB-SAMPLE
Aliquot: 1 x 50 ml
Form: liquid
Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet.
For in vitro laboratory use only
Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA
Tel: (888) 557-5055 - Fax: (248) 896-0148 -