Product Datasheet
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Product Description |
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Product: |
Immobilized soybean trypsin inhibitor |
Catalog Number: |
SBTI-I |
Product Page: |
https://www.mol-innov.com/products/IPLSTIIMM10ML |
Description: |
Soybean trypsin inhibitor (SBTI) is a reversible competitive inhibitor of trypsin and other trypsin-like proteases such as chymotrypsin, plasmin and plasma kallikrein. It has a MW of 22 kDa and a pI of 4.5 (1,2). We have immobilized SBTI on an agarose resin via coupling to primary amines to create an affinity resin with specificity for a number of serine proteases. SBTI binds free trypsin tighter with increasing pH from 4.5-8.0 that allows for a simple gradient to low pH (~2.5-3.0) for elution (1). References: 1. Birk, Y. (1976) Methods Enzymol. 55:700-706. 2. Kassell, B. (1970) Methods Enzymol. 19:853-862. |
Recommended Storage: |
SAMPLE |
Minimum Shelf Life: |
283.50 |
Source: |
5.0 mg/ml coupled |
Molecular Weight: |
Proteins |
Extinction Coefficient: |
21,500 |
Antigen: |
4 C |
Target: |
Resin |
Target Molecular Weight: |
IMMOBILIZED PROTEINS |
Host: |
1256.5 |
Clonality: |
30 |
Isotype: |
Soybean |
Applications: |
Cold pack |
Cross-reactivity: |
12 months from delivery |
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Sample Characteristics |
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Lot Number: |
SBTI-I-IPLSTIIMM50ML |
Aliquot: |
1 x Plant-Derived Soybean Trypsin Inhibitor Purified Immobilized 50ml |
Concentration: |
405 |
Volume: |
1795 |
Specific activity: |
SodiumAzide |
Titer: |
0.1M Tris-HCl; 0.15M NaCl; 0.02% Na Azide; pH 7.4 |
Buffer: |
SAMPLE |
Form: |
10 ml |
Preservative: |
20 ml of 1:1 slurry |
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Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet. |
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For in vitro laboratory use only |
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Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com |