Product Datasheet

 
Product Description
 
Product: Immobilized soybean trypsin inhibitor
Catalog Number: SBTI-I
Product Page: https://www.mol-innov.com/products/IPLSTIIMM10ML
Description: Soybean trypsin inhibitor (SBTI) is a reversible competitive inhibitor of trypsin and other trypsin-like proteases such as chymotrypsin, plasmin and plasma kallikrein. It has a MW of 22 kDa and a pI of 4.5 (1,2). We have immobilized SBTI on an agarose resin via coupling to primary amines to create an affinity resin with specificity for a number of serine proteases. SBTI binds free trypsin tighter with increasing pH from 4.5-8.0 that allows for a simple gradient to low pH (~2.5-3.0) for elution (1). References: 1. Birk, Y. (1976) Methods Enzymol. 55:700-706. 2. Kassell, B. (1970) Methods Enzymol. 19:853-862.
Recommended Storage: SAMPLE
Minimum Shelf Life: 283.50
Source: 5.0 mg/ml coupled
Molecular Weight: Proteins
Extinction Coefficient: 21,500
Antigen: 4 C
Target: Resin
Target Molecular Weight: IMMOBILIZED PROTEINS
Host: 1256.5
Clonality: 30
Isotype: Soybean
Applications: Cold pack
Cross-reactivity: 12 months from delivery
 
Sample Characteristics
 
Lot Number: SBTI-I-IPLSTIIMM50ML
Aliquot: 1 x Plant-Derived Soybean Trypsin Inhibitor Purified Immobilized 50ml
Concentration: 405
Volume: 1795
Specific activity: SodiumAzide
Titer: 0.1M Tris-HCl; 0.15M NaCl; 0.02% Na Azide; pH 7.4
Buffer: SAMPLE
Form: 10 ml
Preservative: 20 ml of 1:1 slurry
 
Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet.
 
For in vitro laboratory use only
 
Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA
Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com