Product Datasheet

 
Product Description
 
Product: Rat uPA total antigen assay ELISA kit
Catalog Number: RUPAKT-TOT
Product Page: https://www.mol-innov.com/products/IRTUPAKTT1KIT
Description: Urokinase plasminogen activator (uPA) is a serine protease that activates plasminogen to plasmin in the blood fibrinolytic system. It is also implicated in events related to cell invasion/migrations. The sensitive quantitative measurement of total rat uPA antigen in plasma and other biological fluid samples is easily performed with this 96 well strip format ELISA kit. The assay measures total uPA in the 0.01-10 ng/ml range. Samples giving rat uPA levels above 10 ng/ml should be diluted in blocking buffer before use. Rat uPA will bind to the capture antibody coated on the microtiter plate. Free and complexed uPA will be detected by the assay. After appropriate washing steps, anti-rat uPA primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of rat uPA in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified rat uPA and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
Suggested additional reagents: 10X Wash Buffer, TMB Substrate, uPA Antigen Capture Plate, Secondary Antibody
Recommended Storage: SAMPLE
Minimum Shelf Life: 346.50
 
Technical Information
 
Purity: Rat
Molecular Weight: ELISA kits
Antigen: 4 C
Target: Complete kit
Target Molecular Weight: uPA ELISA KITS
Host: 1428
Clonality: 30
Isotype: Multiple
Clone: s
Applications: Cold pack
Cross-reactivity: 12 months from manufacture, see label for expiration date
Concentration: 495
Volume: 2040
Buffer: SAMPLE
Form: 1 kit
 
Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet.
 
For in vitro laboratory use only
 
Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA
Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com