Product Datasheet
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Product Description |
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Product: |
Rat Liver Tissue Lysate |
Catalog Number: |
RT-TL-LR |
Product Page: |
https://www.mol-innov.com/products/IRTSDLRTL200UG |
Description: |
A freshly-harvested Sprague Dawley rat liver is flash-frozen in liquid nitrogen within 30 seconds of removal and stored frozen until lysis. The tissue is ground and thoroughly homogenized at 4C with a freshly prepared modified Radio Immuno Precipitation Assay (RIPA) buffer containing strong protease and phosphatase inhibitor cocktails. The final homogenate is incubated with DNase then clarified by centrifugation at 10,000 g at 4C and stored at -80C. Each lot is quality controlled by determining the protein concentration using a BCA assay and analyzed with SDS-PAGE using Coomassie blue for visualization. Sharp, clear protein bands indicate a quality protein lysate. |
Recommended Storage: |
SAMPLE |
Minimum Shelf Life: |
99.75 |
Source: |
2.5 mg/ml |
Molecular Weight: |
Proteins |
Antigen: |
-70 C |
Target: |
Frozen liquid |
Target Molecular Weight: |
ANIMAL TISSUES |
Host: |
156.75 |
Clonality: |
5 |
Isotype: |
Rat liver |
Clone: |
s |
Applications: |
Dry ice |
Cross-reactivity: |
5 years from delivery |
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Sample Characteristics |
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Lot Number: |
RT-TL-LR-IRTSDLRTL400UG |
Aliquot: |
1 x Innovative Grade US Origin Rat Sprague Dawley Liver Tissue Lysate 400ug |
Concentration: |
105 |
Volume: |
165 |
Titer: |
0.1M Tris-HCl; 0.15M NaCl; 0.1% SDS; 0.5% Na Deoxycholate; 1% Triton X-100; pH 7.4 |
Buffer: |
SAMPLE |
Form: |
0.2 mg |
Preservative: |
0.08 ml |
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Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet. |
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For in vitro laboratory use only |
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Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com |