Product Datasheet

Product Description
Product: Rat Prorenin/Renin Total Antigen ELISA Kit
Catalog Number: RPRENKT-TOT
Product Page:
Description: Prorenin is a glycosylated aspartic protease that consists of 2 homologous lobes and is the precursor of renin. Renin activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE, the angiotensin-converting enzyme primarily within the capillaries of the lungs. It has been reported that the levels of circulating prorenin (but not renin) are increased in diabetic subjects. Plasma and serum concentrations increase in several conditions such as pregnancy, progressive diabetes mellitus, diabetes mellitus with microvascular disease, and diabetic retinopathy. The sensitive quantitative measurement of total rat prorenin and renin antigen in plasma, serum or cell culture supernatant samples is easily performed with this 96 well strip format ELISA kit. Renin and prorenin will be detected by the assay. Rat prorenin levels range from 0-400 ng/ml depending on assay methodology. The assay measures total rat prorenin in the 0.1-100 ng/ml range. Samples giving rat prorenin levels above 100 ng/ml should be diluted in blocking buffer before use. Rat prorenin will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-rat prorenin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with avidin conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of prorenin in the samples. A standard calibration curve is prepared using dilutions of purified prorenin and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
Recommended Storage: SAMPLE
Minimum Shelf Life: 332.50
Technical Information
Purity: Rat
Molecular Weight: ELISA kits
Antigen: 4 C
Target: Complete kit
Target Molecular Weight: RENIN/PRORENIN ELISA KITS
Host: 1410.5
Clonality: 30
Isotype: Multiple
Clone: s
Applications: Cold pack
Cross-reactivity: 12 months from manufacture, see label for expiration date
Concentration: 475
Volume: 2015
Buffer: SAMPLE
Form: 1 kit
Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet.
For in vitro laboratory use only
Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA
Tel: (888) 557-5055 - Fax: (248) 896-0148 -