Product Datasheet
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| Product Description |
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| Product: |
Rat Prorenin, C-terminal 8x His tag |
| Catalog Number: |
RPREN-HIS |
| Product Page: |
https://www.mol-innov.com/products/IRTPRENRHIS1MG |
| Description: |
Recombinantly produced in HEK cell culture and purified by chelated metal affinity chromatography. Contains a 8X-Histidine tag at C terminus for purification. Fully activatable to renin by catalytic amounts of trypsin. Prorenin is a glycosylated aspartic protease that consists of 2 homologous lobes and is the precursor of renin. Prorenin exhibits a low level of enzymatic activity relative to renin which is generated from prorenin by proteolytic cleavage of the first ~43 amino acids at the N-terminus. This so called prosegment appears to block the full enzymatic potential of the active site (1). Renin activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE, the angiotensin-converting enzyme primarily within the capillaries of the lungs. It has been reported that the levels of circulating prorenin (but not renin) are increased in diabetic subjects(2).
1) A.H. Jan Danser; Jaap Deinum ; Renin, Prorenin and the Putative (Pro)renin Receptor). Hypertension. 2005;46:1069.
2) Luetscher JA, Kraemer FB, Wilson DM, Schwartz HC, Bryer-Ash M.
Increased plasma inactive renin in diabetes mellitus. A marker of microvascular
complications. N Engl J Med. 1985;312:1412-1417.
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| Recommended Storage: |
SAMPLE |
| Minimum Shelf Life: |
308.00 |
| Source: |
1.0 mg/ml |
| Molecular Weight: |
Proteins |
| Extinction Coefficient: |
41,849 |
| Antigen: |
-70 C |
| Target: |
Frozen liquid |
| Target Molecular Weight: |
RENIN/PRORENIN |
| Host: |
2439.5 |
| Clonality: |
30 |
| Isotype: |
Human Embryonic Kidney cells |
| Applications: |
Dry ice |
| Cross-reactivity: |
3 years from delivery |
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| Sample Characteristics |
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| Lot Number: |
RPREN-HIS-IRTPRENRHIS1MG |
| Aliquot: |
1 x Rat Prorenin Recombinant HIS Labeled 1mg |
| Concentration: |
440 |
| Volume: |
3485 |
| Titer: |
50 mM Tris; pH 8.0 |
| Buffer: |
SAMPLE |
| Form: |
1.0 mg |
| Preservative: |
1.0 ml |
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| Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet. |
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| For in vitro laboratory use only |
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Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA
Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com |