Product Datasheet

 
Product Description
 
Product: Rat PAI-1 total antigen assay ELISA kit
Catalog Number: RPAIKT-TOT
Product Page: https://www.mol-innov.com/products/IRTPAI1KTT1KIT
Description: Plasminogen activator inhibitor type 1 (PAI-1) is involved in the regulation of the blood fibrinolytic system. Increased plasma levels of PAI-1 are implicated in the impairment of fibrinolytic function and may be associated with thrombotic diseases. Levels of PAI-1 increase with age and are elevated in conditions such as normal pregnancy and sepsis. The sensitive quantitative measurement of total rat PAI-1 antigen in plasma samples is easily performed with this 96 well strip format ELISA kit. The concentration level of PAI-1 antigen in rat plasma was found to be 1.8 ng/ml. The assay measures rat PAI-1 in the 0.05-50 ng/ml range. Samples giving rat PAI-1 levels above 50 ng/ml should be diluted in blocking buffer before use. Normal plasma should be applied directly to the plate for best results. It is important to ensure a platelet free preparation of plasma as platelets can release PAI-1. Rat PAI-1 will bind to the capture antibody coated on the microtiter plate. Free, latent, and complexed PAI-1 will be detected by the assay. After appropriate washing steps, biotin labeled anti-rat PAI-1 primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of rat PAI-1 in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified rat PAI-1 and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, Rat PAI-1 Antigen Capture Plate, Secondary Antibody
Recommended Storage: SAMPLE
Minimum Shelf Life: 332.50
 
Technical Information
 
Purity: Rat
Molecular Weight: ELISA kits
Antigen: 4 C
Target: Complete kit
Target Molecular Weight: PAI-1 ELISA KITS
Host: 1410.5
Clonality: 30
Isotype: Multiple
Clone: s
Applications: Cold pack
Cross-reactivity: 12 months from manufacture, see label for expiration date
Concentration: 475
Volume: 2015
Buffer: SAMPLE
Form: 1 kit
 
Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet.
 
For in vitro laboratory use only
 
Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA
Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com