Product Datasheet
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Product Description |
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Product: |
Rat PAI-1 NBD labeled at the reactive center loop |
Catalog Number: |
RPAI-P9NBD |
Product Page: |
https://www.mol-innov.com/products/IRTPAI1RP9NBD500UG |
Description: |
P9-NBD Rat PAI-1 was created by mutagenesis of the P9 serine residue (Ser339) on the reactive center loop to cysteine. This provides a free thiol group for incorporation of N,N'-dimethyl-N-(acetyl)-N'-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) (NBD), a fluorescent probe highly sensitive to changes in solvation and hydrophobic environment. The fluorescence emission of P9-NBD PAI-1 is enhanced 6-7 fold upon insertion of the reactive center loop into beta-sheet A following complex formation with proteinases, formation of the latent species, or cleavage by elastase. The incorporated probe is excited at 480 nm and displays a broad emission spectrum with a peak centered 542 nm with a resultant blue-shift to 520 nm following reactive center loop insertion. This unique product is an ideal reagent for researchers studying PAI-1 behavior in rat models. |
Recommended Storage: |
SAMPLE |
Minimum Shelf Life: |
644.00 |
Source: |
1.0 mg/ml |
Molecular Weight: |
Proteins |
Extinction Coefficient: |
43,000 |
Antigen: |
-70 C; Protect from light |
Target: |
Frozen liquid |
Target Molecular Weight: |
PAI-1 |
Host: |
1218 |
Clonality: |
30 |
Isotype: |
E. coli |
Applications: |
Dry ice |
Cross-reactivity: |
3 years from delivery |
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Sample Characteristics |
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Lot Number: |
RPAI-P9NBD-IRTPAI1RP9NBD1MG |
Aliquot: |
1 x Rat PAI-1 Recombinant P9-NBD Labeled 1 mg |
Concentration: |
920 |
Volume: |
1740 |
Titer: |
0.05M Sodium Acetate; 0.1M NaCl; 1mM EDTA; pH 5.0 |
Buffer: |
SAMPLE |
Form: |
0.5 mg |
Preservative: |
0.5 ml |
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Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet. |
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For in vitro laboratory use only |
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Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com |