Product Datasheet

 
Product Description
 
Product: Rat PAI-1 NBD labeled at the reactive center loop
Catalog Number: RPAI-P9NBD
Product Page: https://www.mol-innov.com/products/IRTPAI1RP9NBD500UG
Description: P9-NBD Rat PAI-1 was created by mutagenesis of the P9 serine residue (Ser339) on the reactive center loop to cysteine. This provides a free thiol group for incorporation of N,N'-dimethyl-N-(acetyl)-N'-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) (NBD), a fluorescent probe highly sensitive to changes in solvation and hydrophobic environment. The fluorescence emission of P9-NBD PAI-1 is enhanced 6-7 fold upon insertion of the reactive center loop into beta-sheet A following complex formation with proteinases, formation of the latent species, or cleavage by elastase. The incorporated probe is excited at 480 nm and displays a broad emission spectrum with a peak centered 542 nm with a resultant blue-shift to 520 nm following reactive center loop insertion. This unique product is an ideal reagent for researchers studying PAI-1 behavior in rat models.
Recommended Storage: SAMPLE
Minimum Shelf Life: 644.00
Source: 1.0 mg/ml
Molecular Weight: Proteins
Extinction Coefficient: 43,000
Antigen: -70 C; Protect from light
Target: Frozen liquid
Target Molecular Weight: PAI-1
Host: 1218
Clonality: 30
Isotype: E. coli
Applications: Dry ice
Cross-reactivity: 3 years from delivery
 
Sample Characteristics
 
Lot Number: RPAI-P9NBD-IRTPAI1RP9NBD1MG
Aliquot: 1 x Rat PAI-1 Recombinant P9-NBD Labeled 1 mg
Concentration: 920
Volume: 1740
Titer: 0.05M Sodium Acetate; 0.1M NaCl; 1mM EDTA; pH 5.0
Buffer: SAMPLE
Form: 0.5 mg
Preservative: 0.5 ml
 
Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet.
 
For in vitro laboratory use only
 
Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA
Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com