Product Datasheet
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| Product Description |
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| Product: |
Human RAP Stable Mutant |
| Catalog Number: |
RAP-SM |
| Product Page: |
https://www.mol-innov.com/products/IHURAPRSM500UG |
| Description: |
This stable mutant form of human Receptor Associated Protein (RAP) is resistant to both pH and heat induced denaturation. The molecule binds to lipoprotein receptor-related protein 1 (LRP1) with high affinity and inhibits LRP1 function both in vitro and in vivo. The D3 domain of RAP, which unfolds at low pH causing dissociation from LRP1, has been stabilized with six mutations (1). Y260 and T297 are changed to cysteines to generate a novel disulfide bond between helices 2 and 3 while H257, H259, H268, and H290 are changed to phenylalanines. Human RAP Stable Mutant does not unfold at pH 5.5 and has a melting temperature of 73°C, more than 30 degrees above that of wild type RAP. It is also more resistant to trypsin and chymotrypsin mediated proteolysis. Human RAP Stable Mutant may be useful in multiple pathological settings where LRP1 blockade has shown to be effective. Produced recombinantly in E. coli. Endotoxin reduced by reverse-phase chromatography for cell based experiments and animal studies.
References
1. Joni M. Prasad, Mary Migliorini, Rebeca Galisteo, and Dudley K. Strickland. Generation of a Potent Low Density Lipoprotein Receptor-related Protein 1 (LRP1) Antagonist by Engineering a Stable Form of the Receptor-associated Protein (RAP) D3 Domain. J. Biol. Chem. 2015 290: 17262-17268. |
| Recommended Storage: |
SAMPLE |
| Minimum Shelf Life: |
500.50 |
| Source: |
2.5 mg/ml |
| Molecular Weight: |
Proteins |
| Extinction Coefficient: |
39,000 |
| Antigen: |
-70 C |
| Target: |
Frozen liquid |
| Target Molecular Weight: |
RAP (RECEPTOR ASSOCIATED PROTEIN) |
| Host: |
906.5 |
| Clonality: |
30 |
| Isotype: |
E. coli |
| Applications: |
Dry ice |
| Cross-reactivity: |
3 years from delivery |
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| Sample Characteristics |
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| Lot Number: |
RAP-SM-IHURAPRSM1MG |
| Aliquot: |
1 x Human Receptor-Associated Protein Recombinant Stable Mutant 1mg |
| Concentration: |
715 |
| Volume: |
1295 |
| Titer: |
0.02M HEPES; 0.15M NaCl; 0.001M CaCl2; pH 7.5 |
| Buffer: |
February, 2016 |
| Form: |
0.5 mg |
| Preservative: |
0.2 ml |
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| Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet. |
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| For in vitro laboratory use only |
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Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA
Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com |