Product Datasheet

 
Product Description
 
Product: TaqRobat Native Taq MantisGreen 2X Master Mix
Catalog Number: NTAQGREEN
Product Page: https://www.mol-innov.com/products/
Description: TaqRobat Native Taq MantisGreen 2X Master Mix is an optimized, ready-to-use mix that contains dNTPs, buffers, stabilizers, MantisGreen loading dyes and our TaqRobat recombinant Taq DNA polymerase. Reactions can go directly from PCR tube to gel. The mix is a 2X formulation that requires only the addition of primers, template and water. MantisGreen dual-color loading dyes do not inhibit PCR and make monitoring progress easy during electrophoresis. The dyes run outside the range of typical PCR products and therefore do not obscure visualization. The blue dye runs at 4kb and the yellow dye runs under 25bp. TaqRobat 2X Master Mix provides robust amplification over a wide range of templates up to 5kb. Suitable for direct-to-gel Standard Endpoint PCR, Colony PCR, Low Copy PCR, Genotyping, and TA Cloning. Also available without loading dyes.

Reaction setup: The following setup is recommended for a 50µl reaction but can vary depending on the template and primers being used.
ComponentVolumeFinal Concentration
Master Mix25µl1X
5' Primer, 10µM0.5-5µl0.1-1µM
3' Primer, 10µM0.5-5µl0.1-1µM
DNA Template0.1-5µl>1ng
Nuclease Free WaterQS to 50µl


Thermal cycling conditions: The following general cycling conditions are recommended but can vary depending on the template and primers being used.
Cycling StepTemperatureHolding TimeCycles
Initial Denaturation94°C30sec-2min1
Denaturation94-96°C15-30sec20-30
Annealing*55-65°C15-60sec
Extension70-72°C1min per kb
Final Extension70-72°C0-10min1
*Annealing temperature will depend on primer length and composition. Generally, begin 5°C below primer Tm.
Recommended Storage: SAMPLE
Minimum Shelf Life: 213.75
Molecular Weight: Proteins
Antigen: -20 C
Target: Liquid
Target Molecular Weight: MOLECULAR BIOLOGY REAGENTS
Host: 413.25
Clonality: 5
Isotype: E. coli
Applications: Dry ice
Concentration: 225
Volume: 435
Buffer: January, 2016
Form: 500 reactions
Preservative: 12.5 ml/vial
 
Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet.
 
For in vitro laboratory use only
 
Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA
Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com