Product Datasheet

 
Product Description
 
Product: TaqRobat Native Taq DNA Polymerase
Catalog Number: NTAQ
Product Page: https://www.mol-innov.com/products/taqrobat-native-taq-dna-polymerase
Description: TaqRobat Native Taq DNA Polymerase is a recombinant Taq that has identical characteristics to native Taq from Thermus aquaticus. This highly robust enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa, and will create products with dA overhangs at 3' ends. Suitable for Standard Endpoint PCR, Colony PCR, Genotyping, and TA Cloning of DNA templates up to 5kb. TaqRobat offers higher yields than some leading competitors with excellent lot-to-lot reproducibility. TaqRobat undergoes extensive quality control and is free of contaminating endonucleases.

Supplied with your choice of complimentary MgCl2 or MgCl2 free 10X PCR reaction buffer.

Thermal cycling conditions: The following general cycling conditions are recommended but can vary depending on the template and primers being used.
Cycling StepTemperatureHolding TimeCycles
Initial Denaturation94°C2min1
Denaturation94-96°C30sec-4min20-30
Annealing*55-65°C15-30sec
Extension70-72°C30sec-1min per kb
Final Extension70-72°C0-10min1
*Annealing temperature will depend on primer length and composition. Generally, begin 5°C below primer Tm.
Recommended Storage: -20 C
Shipping Conditions: Dry ice
Source: E. coli
Molecular Weight: 94,000
 
Sample Characteristics
 
Lot Number: NTAQ-SAMPLE
Aliquot: 1 x 500 units
Concentration: 5 U/ul
Volume: 0.1 ml
Buffer: 0.02M HEPES; 0.1M KCl; 0.1mM EDTA; 1mM DTT; 50% Glycerol; pH 7.9
Form: Liquid
 
Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet.
 
For in vitro laboratory use only
 
Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA
Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com