Product Datasheet
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| Product Description |
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| Product: |
TaqRobat Native Taq DNA Polymerase |
| Catalog Number: |
NTAQ |
| Product Page: |
https://www.mol-innov.com/products/ |
| Description: |
TaqRobat Native Taq DNA Polymerase is a recombinant Taq that has identical characteristics to native Taq from Thermus aquaticus. This highly robust enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa, and will create products with dA overhangs at 3' ends. Suitable for Standard Endpoint PCR, Colony PCR, Genotyping, and TA Cloning of DNA templates up to 5kb. TaqRobat offers higher yields than some leading competitors with excellent lot-to-lot reproducibility. TaqRobat undergoes extensive quality control and is free of contaminating endonucleases.
Supplied with your choice of complimentary MgCl2 or MgCl2 free 10X PCR reaction buffer.
Thermal cycling conditions: The following general cycling conditions are recommended but can vary depending on the template and primers being used.
| Cycling Step | Temperature | Holding Time | Cycles |
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| Initial Denaturation | 94°C | 2min | 1 |
| Denaturation | 94-96°C | 30sec-4min | 20-30 |
| Annealing* | 55-65°C | 15-30sec |
| Extension | 70-72°C | 30sec-1min per kb |
| Final Extension | 70-72°C | 0-10min | 1 |
*Annealing temperature will depend on primer length and composition. Generally, begin 5°C below primer Tm. |
| Recommended Storage: |
SAMPLE |
| Minimum Shelf Life: |
114.00 |
| Source: |
5 U/ul |
| Molecular Weight: |
Proteins |
| Extinction Coefficient: |
94,000 |
| Antigen: |
-20 C |
| Target: |
Liquid |
| Target Molecular Weight: |
MOLECULAR BIOLOGY REAGENTS |
| Host: |
199.5 |
| Clonality: |
5 |
| Isotype: |
E. coli |
| Applications: |
Dry ice |
| Concentration: |
120 |
| Volume: |
210 |
| Titer: |
0.02M HEPES; 0.1M KCl; 0.1mM EDTA; 1mM DTT; 50% Glycerol; pH 7.9 |
| Buffer: |
January, 2016 |
| Form: |
500 units |
| Preservative: |
0.1 ml |
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| Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet. |
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| For in vitro laboratory use only |
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Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA
Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com |