Product Datasheet

 
Product Description
 
Product: Mouse Immunoglobulin M ELISA Kit
Catalog Number: MSIGMKT
Product Page: https://www.mol-innov.com/products/IMSIGMKT1KIT
Description: Immunoglobulin M (IgM) is the first immunoglobulin produced in the immune response and is the third most abundant immunoglobulin in serum. IgM is a disulfide-linked 970kDa pentamer that activates complement and is responsible for red blood cell agglutination. Each monomer consists of two mu heavy chains and two kappa or lambda light chains. The sensitive quantitative measurement of total mouse IgM antigen in serum, plasma, hybridoma cell supernatants, ascites or other biological fluid samples is easily performed with this 96 well strip format ELISA kit. The concentration of IgM in normal mouse serum ranges from 0.5 to 2.0 mg/ml. The assay measures mouse IgM in the 0.5-500 ng/ml range. Samples giving mouse IgM levels above 500 ng/ml should be diluted in blocking buffer before use. A 1:5,000 to 1:10,000 dilution for normal mouse serum or plasma is suggested for best results. Mouse IgM will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, peroxidase labeled anti-mouse IgM primary antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of mouse IgM in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified mouse IgM and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
Recommended Storage: SAMPLE
Minimum Shelf Life: 252.00
 
Technical Information
 
Purity: Mouse
Molecular Weight: ELISA kits
Antigen: 4 C
Target: Complete kit
Target Molecular Weight: IMMUNOGLOBULIN ELISA KITS
Host: 1211
Clonality: 30
Isotype: Multiple
Applications: Cold pack
Cross-reactivity: 12 months from manufacture, see label for expiration date
Concentration: 360
Volume: 1730
Buffer: June, 2015
Form: 1 kit
 
Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet.
 
For in vitro laboratory use only
 
Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA
Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com