Product Datasheet

Product Description
Product: Mouse Cerebral Cortex Tissue Lysate
Catalog Number: MS-TL-CC
Product Page:
Description: A freshly-harvested Balb C mouse brain is dissected and the cerebral cortex is flash-frozen in liquid nitrogen and stored frozen until lysis. The tissue is ground and thoroughly homogenized at 4C with a freshly prepared modified Radio Immuno Precipitation Assay (RIPA) buffer containing strong protease and phosphatase inhibitor cocktails. The final homogenate is incubated with DNase then clarified by centrifugation at 10,000 g at 4C and stored at -80C. Each lot is quality controlled by determining the protein concentration using a BCA assay and analyzed with SDS-PAGE using Coomassie blue for visualization. Sharp, clear protein bands indicate a quality protein lysate.
Recommended Storage: SAMPLE
Minimum Shelf Life: 99.75
Source: 2.5 mg/ml
Molecular Weight: Proteins
Antigen: -70 C
Target: Frozen liquid
Target Molecular Weight: ANIMAL TISSUES
Host: 156.75
Clonality: 5
Isotype: Mouse brain
Clone: s
Applications: Dry ice
Cross-reactivity: 5 years from delivery
Sample Characteristics
Aliquot: 1 x Innovative Grade US Origin Mouse Cerebral Cortex Tissue Lysate 400ug
Concentration: 105
Volume: 165
Titer: 0.1M Tris-HCl; 0.15M NaCl; 0.1% SDS; 0.5% Na Deoxycholate; 1% Triton X-100; pH 7.4
Buffer: SAMPLE
Form: 0.2 mg
Preservative: 0.08 ml
Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet.
For in vitro laboratory use only
Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA
Tel: (888) 557-5055 - Fax: (248) 896-0148 -