Product Datasheet

Product Description
Product: Mouse Cerebellum Tissue Lysate
Catalog Number: MS-TL-CB
Product Page:
Description: A freshly-harvested Balb C mouse brain is dissected and the cerebellum is flash-frozen in liquid nitrogen and stored frozen until lysis. The tissue is ground and thoroughly homogenized at 4C with a freshly prepared modified Radio Immuno Precipitation Assay (RIPA) buffer containing strong protease and phosphatase inhibitor cocktails. The final homogenate is incubated with DNase then clarified by centrifugation at 10,000 g at 4C and stored at -80C. Each lot is quality controlled by determining the protein concentration using a BCA assay and analyzed with SDS-PAGE using Coomassie blue for visualization. Sharp, clear protein bands indicate a quality protein lysate.
Recommended Storage: -70 C
Shipping Conditions: Dry ice
Minimum Shelf Life: 5 years from delivery
Source: Mouse brain
Sample Characteristics
Aliquot: 1 x 0.2 mg
Concentration: 2.5 mg/ml
Volume: 0.08 ml
Buffer: 0.1M Tris-HCl; 0.15M NaCl; 0.1% SDS; 0.5% Na Deoxycholate; 1% Triton X-100; pH 7.4
Form: Frozen liquid
Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet.
For in vitro laboratory use only
Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA
Tel: (888) 557-5055 - Fax: (248) 896-0148 -