Product Datasheet
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| Product Description |
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| Product: |
Mouse IL-2, chiMAX Fc Fusion Protein |
| Catalog Number: |
MIL2-FC |
| Product Page: |
https://www.mol-innov.com/products/IMSIL2CFC50UG |
| Description: |
Interleukin-2 (IL-2) is a O-glycosylated
four alpha-helix bundle cytokine that is produced by T-cells in response to antigenic or mitogenic stimulation. IL-2 can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells and is required for T-cell proliferation and other activities crucial to regulation of the immune response. Mouse IL-2 (Ala21-Gln169) is expressed recombinantly, purified by chromatography, sterile filtered and lyophilized. >95percent pure by SDS-PAGE and biologically active as measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T-cells. (Gearing and Bird (1987) Production and assay of interleukin 2. In Lymphokines and Interferons: A Practical Approach (Clemens et al. eds.) pp. 291-301). The ED50 for this effect is typically < 1.0 ng/ml. Add deionized water to original volume, aliquot and freeze unused portion.
This chiMAX Fc Fusion Protein is glycosylated and expressed as a chimera with the Fc region of mouse immunoglobulin (heavy chain hinge, CH2 and CH3). Fc fusion proteins are typically more stable and resistant to degradation and clearance than native cytokines. Fc fusion proteins appear as a dimer in SDS-PAGE under non-reducing conditions.
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| Recommended Storage: |
SAMPLE |
| Shipping Conditions: |
ED50 < 5 ng/ml as determined in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. |
| Minimum Shelf Life: |
91.00 |
| Source: |
Lyophilized |
| Molecular Weight: |
Proteins |
| Extinction Coefficient: |
43,000 |
| Antigen: |
-70 C |
| Target: |
Lyophilized powder |
| Target Molecular Weight: |
INTERLEUKINS AND INTERLEUKIN RECEPTORS |
| Host: |
1288 |
| Clonality: |
30 |
| Isotype: |
Insect cell culture |
| Clone: |
s |
| Applications: |
Cold pack |
| Cross-reactivity: |
12 months from delivery |
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| Sample Characteristics |
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| Lot Number: |
MIL2-FC-IMSIL2CFC1MG |
| Aliquot: |
1 x Mouse Interleukin-2 (IL-2) Purified chiMAX Fc Fusion 1mg |
| Concentration: |
130 |
| Volume: |
1840 |
| Titer: |
PBS, 0.1% BSA |
| Buffer: |
SAMPLE |
| Form: |
0.05 mg |
| Preservative: |
0.025 ml before lyophilization |
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| Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet. |
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| For in vitro laboratory use only |
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Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA
Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com |