| Description: |
Hydrolyzes peptides, amides, and ester bonds involving the carboxyl groups of L-arginine or L-lysine. Increased serum values of this enzyme and/or its zymogen (trypsinogen) have been found in patients with cystic fibrosis. Trypsinogen has three isoforms: cationic (trypsinogen 1), anionic (trypsinogen 2, MW 22,500), and mesotrypsinogen (trypsinogen 3, MW, 25,000). This trypsin is the cationic form. Activity: 2.5 units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one umole of N-benzoyl-DL-arginine-pNA per minute at 25oC in 200 mM Tris-HCl, pH 7.8, with 20 mM CaCl2. Prepared from tissue shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. Add deionized water to original volume, aliquot and freeze unused portion. |
