Product Datasheet
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| Product Description |
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| Product: |
TaqTivate Hot Start Taq MantisGreen Master Mix |
| Catalog Number: |
HTAQGREEN |
| Product Page: |
https://www.mol-innov.com/products/ |
| Description: |
TaqTivate Hot Start Taq MantisGreen Master Mix is an optimized, ready-to-use mix that contains dNTPs, MgCl2, optimized buffers, MantisGreen loading dyes and our TaqTivate recombinant hot start Taq DNA polymerase. Reactions can go directly from PCR tube to gel. The mix is a 2X formulation that requires only the addition of primers, template and water. MantisGreen dual-color loading dyes do not inhibit PCR and make monitoring progress easy during electrophoresis. The dyes run outside the range of typical PCR products and therefore do not obscure visualization. The blue dye runs at 4kb and the yellow dye runs under 25bp. TaqTivate requires a 95C activation time of only two minutes for faster cycling reactions. Chemical modification keeps TaqTivate free of contaminating DNA from biological modifying agents such as antibodies. TaqTivate 2X Master Mix provides robust amplification of templates up to 5kb and reduces non-specific DNA amplification due to primer-dimer formation. Suitable for direct-to-gel Standard Endpoint PCR, Colony PCR, Low Copy PCR, Genotyping, and TA Cloning. Also available without loading dyes.
Reaction setup: The following setup is recommended for a 50µl reaction but can vary depending on the template and primers being used.
| Component | Volume | Final Concentration |
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| Master Mix | 25µl | 1X | | 5' Primer, 10µM | 0.5-5µl | 0.1-1µM | | 3' Primer, 10µM | 0.5-5µl | 0.1-1µM | | DNA Template | 0.1-5µl | >1ng | | Nuclease Free Water | QS to 50µl |
Thermal cycling conditions: The following general cycling conditions are recommended but can vary depending on the template and primers being used.
| Cycling Step | Temperature | Holding Time | Cycles |
|---|
Initial Denaturation
and Hot Start Activation | 94°C | 30sec-2min | 1 | | Denaturation | 94-96°C | 15-30sec | 20-30 | | Annealing* | 55-65°C | 15-60sec | | Extension | 70-72°C | 1min per kb | | Final Extension | 70-72°C | 0-10min | 1 | *Annealing temperature will depend on primer length and composition. Generally, begin 5°C below primer Tm. |
| Recommended Storage: |
SAMPLE |
| Minimum Shelf Life: |
114.00 |
| Molecular Weight: |
Proteins |
| Antigen: |
-20 C |
| Target: |
Liquid |
| Target Molecular Weight: |
MOLECULAR BIOLOGY REAGENTS |
| Host: |
394.25 |
| Clonality: |
5 |
| Isotype: |
E. coli |
| Applications: |
Dry ice |
| Concentration: |
120 |
| Volume: |
415 |
| Buffer: |
January, 2016 |
| Form: |
100 reactions |
| Preservative: |
2.5 ml/vial |
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| Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet. |
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| For in vitro laboratory use only |
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Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA
Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com |