Product Datasheet

 
Product Description
 
Product: Human PAI-1 (substrate form - P12 Arginine P14 Arginine double mutant)
Catalog Number: HPAI-RR
Product Page: https://www.mol-innov.com/products/IHUPAI1RP12P14SB500UG
Description: Two amino acid substitutions at positions P12 and P14 in the reactive center loop produces a PAI-1 that becomes a substrate for proteinases rather than an inhibitor. The reactive center loop does not insert following protease cleavage and the PAI-1 retains normal vitronectin binding. The double mutation also results in extended half-life compared to native PAI-1. This molecule is useful for mechanistic studies.
References
1. Huang, Y. et al. (2009) Am. J. Physiol. Renal Physiol. 297:F1045–F1054.
2. Courey, A.J. et al. (2011) Blood. 118:2313–2321.
3. Zhong, J. et al. (2014) Lab. Invest. 94:633–644.
Recommended Storage: SAMPLE
Minimum Shelf Life: 500.50
Source: 2.5 mg/ml
Molecular Weight: Proteins
Extinction Coefficient: 43,000
Antigen: -70 C
Target: Frozen liquid
Target Molecular Weight: PAI-1
Host: 819
Clonality: 30
Isotype: E. coli
Applications: Dry ice
Cross-reactivity: 3 years from delivery
 
Sample Characteristics
 
Lot Number: HPAI-RR-IHUPAI1RP12P14SB1MG
Aliquot: 1 x Human PAI-1 Recombinant P12/P14 Arginine Double Mutant Substrate 1 mg
Concentration: 715
Volume: 1170
Titer: 0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; pH 6.6
Buffer: SAMPLE
Form: 0.5 mg
Preservative: 0.2 ml
 
Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet.
 
For in vitro laboratory use only
 
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Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com