Product Datasheet
|
|
|
Product Description |
|
Product: |
Human PAI-1 (substrate form - P12 Arginine P14 Arginine double mutant) |
Catalog Number: |
HPAI-RR |
Product Page: |
https://www.mol-innov.com/products/IHUPAI1RP12P14SB500UG |
Description: |
Two amino acid substitutions at positions P12 and P14 in the reactive center loop produces a PAI-1 that becomes a substrate for proteinases rather than an inhibitor. The reactive center loop does not insert following protease cleavage and the PAI-1 retains normal vitronectin binding. The double mutation also results in extended half-life compared to native PAI-1. This molecule is useful for mechanistic studies.
References
1. Huang, Y. et al. (2009) Am. J. Physiol. Renal Physiol. 297:F1045–F1054.
2. Courey, A.J. et al. (2011) Blood. 118:2313–2321.
3. Zhong, J. et al. (2014) Lab. Invest. 94:633–644. |
Recommended Storage: |
SAMPLE |
Minimum Shelf Life: |
500.50 |
Source: |
2.5 mg/ml |
Molecular Weight: |
Proteins |
Extinction Coefficient: |
43,000 |
Antigen: |
-70 C |
Target: |
Frozen liquid |
Target Molecular Weight: |
PAI-1 |
Host: |
819 |
Clonality: |
30 |
Isotype: |
E. coli |
Applications: |
Dry ice |
Cross-reactivity: |
3 years from delivery |
|
Sample Characteristics |
|
Lot Number: |
HPAI-RR-IHUPAI1RP12P14SB1MG |
Aliquot: |
1 x Human PAI-1 Recombinant P12/P14 Arginine Double Mutant Substrate 1 mg |
Concentration: |
715 |
Volume: |
1170 |
Titer: |
0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; pH 6.6 |
Buffer: |
SAMPLE |
Form: |
0.5 mg |
Preservative: |
0.2 ml |
|
Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet. |
|
For in vitro laboratory use only |
|
Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com |