Product Datasheet
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| Product Description |
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| Product: |
HB101 Competent Cells |
| Catalog Number: |
HB101 |
| Product Page: |
https://www.mol-innov.com/products/ |
| Description: |
HB101 strain chemically competent cells from Molecular Innovations are an all purpose reagent for general cloning, sub-cloning and plasmid isolation. For a transformation efficiency of at least 1x106 simply thaw a tube of cells on ice, add 1-5 ul of DNA, gently mix and incubate on ice for 2-5 minutes, then spread 50-100 ul onto a pre-warmed culture plate. For greater transformation efficiencies heat shock at 37C for 1 minute before plating, or heat shock at 42C for 30 seconds then add SOC media and shake at 225 rpm at 37C for 1 hour before plating. Conveniently supplied as a pack of 5 single use 0.2 ml aliquots.
Genotype: F- mcrB mrr hsdS20(rB- mB-) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20(SmR) glnV44 λ- |
| Recommended Storage: |
SAMPLE |
| Minimum Shelf Life: |
95.00 |
| Molecular Weight: |
Reagents |
| Antigen: |
-70 C |
| Target: |
Frozen liquid |
| Target Molecular Weight: |
MOLECULAR BIOLOGY REAGENTS |
| Host: |
180.5 |
| Clonality: |
5 |
| Isotype: |
E. coli |
| Applications: |
Dry ice |
| Concentration: |
100 |
| Volume: |
190 |
| Buffer: |
January, 2016 |
| Form: |
1.0 ml |
| Preservative: |
10 x 0.1 ml |
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| Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet. |
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| For in vitro laboratory use only |
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Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA
Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com |