Product Datasheet

 
Product Description
 
Product: DH5-alpha Competent Cells
Catalog Number: DH5A
Product Page: https://www.mol-innov.com/products/IECDH5ACC1ML
Description: DH5-alpha strain chemically competent cells are an all purpose reagent for general cloning, sub-cloning and plasmid isolation. For a transformation efficiency of at least 1x106 simply thaw a tube of cells on ice, add 1-5 ul of DNA, gently mix and incubate on ice for 2-5 minutes, then spread 50-100 ul onto a pre-warmed culture plate. For greater transformation efficiencies heat shock at 37C for 1 minute before plating, or heat shock at 42C for 30 seconds then add SOC media and shake at 225 rpm at 37C for 1 hour before plating. Conveniently supplied as a pack of 10 single use 0.1 ml aliquots.

Genotype: F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 λ-
Recommended Storage: SAMPLE
Minimum Shelf Life: 95.00
Molecular Weight: Reagents
Antigen: -70 C
Target: Frozen liquid
Target Molecular Weight: MOLECULAR BIOLOGY REAGENTS
Host: 180.5
Clonality: 5
Isotype: E. coli
Applications: Dry ice
Concentration: 100
Volume: 190
Buffer: 9-Apr-19
Form: 1.0 ml
Preservative: 10 x 0.1 ml
 
Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet.
 
For in vitro laboratory use only
 
Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA
Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com