Product Datasheet

 
Product Description
 
Product: Human CCL2
Catalog Number: CCL2
Product Page: https://www.mol-innov.com/products/IHUCCL2LY25UG
Description: C-C motif chemokine 2 (CCL2) is a 76 amino acid Cys-Cys chemokine that has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It is also known as monocyte chemoattractant protein 1 (MCP1), monocyte chemotactic and activating factor (MCAF), and small inducible cytokine A2. CCL2 is a ligand for the G protein coupled receptors CCR2 and CCR4, and induces chemotactic migration of monocytes, activated T cells, basophils, NK cells and dendritic cells, but not neutrophils or eosinophils. CCL2 can augment monocyte anti-tumor activity and may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC chemokines are chemotactic cytokines characterized by two adjacent cysteines that induce directed chemotaxis in nearby responsive cells. Recombinant human CCL2 from Molecular Innovations is provided as a native untagged protein. This chemokine is expressed in E. coli then processed, refolded and purified to yield the native, secreted form of the mature chemokine. >98percent pure by SDS-PAGE, HPLC, MALDI-MS and NMR analysis. CCR2 activity verified by intracellular calcium flux using THP1 cells as described by Veldkamp et al, Science Signaling 2008. Add deionized water to desired volume, aliquot and freeze unused portion.
Recommended Storage: SAMPLE
Minimum Shelf Life: 216.00
Source: Lyophilized
Molecular Weight: Proteins
Extinction Coefficient: 8,681
Antigen: -20 C
Target: Lyophilized powder
Target Molecular Weight: CHEMOKINES
Host: 568
Clonality: 20
Isotype: E. coli
Applications: Cold pack
Cross-reactivity: 3 years from delivery
Concentration: 270
Volume: 710
Titer: Salt free
Buffer: June, 2016
Form: 0.025 mg
 
Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet.
 
For in vitro laboratory use only
 
Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA
Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com