Product Datasheet
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Product Description |
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Product: |
BL21(DE3) Competent Cells |
Catalog Number: |
BL21 |
Product Page: |
https://www.mol-innov.com/products/IECBL21CC1ML |
Description: |
BL21(DE3) strain chemically competent cells from Molecular Innovations are an all purpose reagent for T7 promoter driven IPTG inducible E. coli protein expression. For greater transformation efficiencies heat shock at 37C for 1 minute before plating, or heat shock at 42C for 30 seconds then add SOC media and shake at 225 rpm at 37C for 1 hour before plating. Conveniently supplied as a pack of 10 single use 0.1 ml aliquots.
Genotype: E. coli str. B F- ompT gal dcm lon hsdS20(rB- mB-) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) |
Recommended Storage: |
SAMPLE |
Minimum Shelf Life: |
95.00 |
Molecular Weight: |
Reagents |
Antigen: |
-70 C |
Target: |
Frozen liquid |
Target Molecular Weight: |
MOLECULAR BIOLOGY REAGENTS |
Host: |
180.5 |
Clonality: |
5 |
Isotype: |
E. coli |
Applications: |
Dry ice |
Concentration: |
100 |
Volume: |
190 |
Buffer: |
29-Sep-17 |
Form: |
1.0 ml |
Preservative: |
10 x 0.1 ml |
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Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet. |
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For in vitro laboratory use only |
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Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com |