Product Datasheet
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| Product Description |
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| Product: |
Anhydrotrypsin |
| Catalog Number: |
ANHT |
| Product Page: |
https://www.mol-innov.com/products/IBOAHTRAP100UG |
| Description: |
Treatment of trypsin with phenylmethylsulfonyl fluoride (PMSF) followed
by a strong base leads to the conversion of Serine 195 to dehydroalanine
at the active site (1). Alpha and beta forms of anhydrotrypsin are
separated by immobilized soybean trypsin inhibitor. The activity of
anhydrotrypsin is greatly reduced compared to native trypsin as
quantified by chromogenic substrate (2). Anhydrotrypsin provided by
our company is the single chain beta-form prepared from Type
XIII bovine trypsin.
References
1. Ako, H. et al. (1972) Biochem Biophys Res Commun. 47:1402-1407.
2. Olson, S.T. et al. (1995) J. Biol. Chem. 270:30007-30017. |
| Recommended Storage: |
SAMPLE |
| Minimum Shelf Life: |
245.00 |
| Source: |
2.0 mg/ml |
| Molecular Weight: |
Proteins |
| Extinction Coefficient: |
23,300 |
| Antigen: |
-70 C |
| Target: |
Frozen liquid |
| Target Molecular Weight: |
ANHYDROTRYPSIN |
| Host: |
1953 |
| Clonality: |
30 |
| Isotype: |
Bovine pancreas |
| Applications: |
Dry ice |
| Cross-reactivity: |
3 years from delivery |
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| Sample Characteristics |
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| Lot Number: |
ANHT-IBOAHTRAP1MG |
| Aliquot: |
1 x Bovine Anhydrotrypsin Affinity Purified 1mg |
| Concentration: |
350 |
| Volume: |
2790 |
| Titer: |
1mM HCl; 10mM CaCl2 |
| Buffer: |
SAMPLE |
| Form: |
0.1 mg |
| Preservative: |
0.05 ml |
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| Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet. |
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| For in vitro laboratory use only |
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Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA
Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com |