Rat Prorenin, C-terminal 8x His tag

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Rat Prorenin, C-terminal 8x His tag

Recombinantly produced in HEK cell culture and purified by chelated metal affinity chromatography. Contains a 8X-Histidine tag at C terminus for purification. Fully activatable to renin by catalytic amounts of trypsin. Prorenin is a glycosylated aspartic protease that consists of 2 homologous lobes and is the precursor of renin. Prorenin exhibits a low level of enzymatic activity relative to renin which is generated from prorenin by proteolytic cleavage of the first ~43 amino acids at the N-terminus. This so called prosegment appears to block the full enzymatic potential of the active site (1). Renin activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE, the angiotensin-converting enzyme primarily within the capillaries of the lungs. It has been reported that the levels of circulating prorenin (but not renin) are increased in diabetic subjects(2).

1) A.H. Jan Danser; Jaap Deinum ; Renin, Prorenin and the Putative (Pro)renin Receptor). Hypertension. 2005;46:1069.

2) Luetscher JA, Kraemer FB, Wilson DM, Schwartz HC, Bryer-Ash M.
Increased plasma inactive renin in diabetes mellitus. A marker of microvascular
complications. N Engl J Med. 1985;312:1412-1417.

Gene ID: 24715
Swiss-Prot/UniProt ID: P08424

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1. Batenburg WW, Lu X, Leijten F et-al. Renin- and prorenin-induced effects in rat vascular smooth muscle cells overexpressing the human (pro)renin receptor: does (pro)renin-(pro)renin receptor interaction actually occur? Hypertension. 2011;58 (6): 1111-9. doi:10.1161/HYPERTENSIONAHA.111.180737Pubmed citation

2. Zhu, Tong et al. ‘Prorenin Stimulates A Pro-Angiogenic And Pro-Inflammatory Response In Retinal Endothelial Cells And An M1 Phenotype In Retinal Microglia’. Clin Exp Pharmacol Physiol 42.5 (2015): 537-548. Web. 5 June 2015. Link to article