Rat PAI-1 NBD labeled at the scissile bond of the reactive center loop
P1′-NBD rat PAI-1 was created by mutagenesis of the methionine residue (Met348) at the P1-P1′ scissile bond to cysteine. This provides a free thiol group for labeling with NBD, a fluorescent probe highly sensitive to changes in solvation and hydrophobic environment. The fluorescence emission of P1′-NBD PAI-1 is quenched upon cleavage of the reactive center loop by a target proteinase. This unique product is an ideal reagent for researchers studying PAI-1 behavior in rat models.