Non-enzymatic Mouse tPA

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Product Description

Non-enzymatic Mouse tPA

This single chain mouse tPA has the active site serine mutated to alanine rendering the enzyme catalytically inactive. The mutation site is S481A on the mature protein and S510A on the complete mRNA sequence (UniProtKB: locus TPA_MOUSE, accession P11214). The tPA still retains exosite binding as well as other biological properties of tPA including but not limited to surface binding to fibrin. This protein is not sterile and should be passed through a 0.22 micron filter before use in cell culture.

Gene ID: 18791
Swiss-Prot/UniProt ID: P11214

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1. Hu K, Yang J, Tanaka S et-al. Tissue-type plasminogen activator acts as a cytokine that triggers intracellular signal transduction and induces matrix metalloproteinase-9 gene expression. J. Biol. Chem. 2006;281 (4): 2120-7. doi:10.1074/jbc.M504988200Pubmed citation

2. Parathath SR, Parathath S, Tsirka SE. Nitric oxide mediates neurodegeneration and breakdown of the blood-brain barrier in tPA-dependent excitotoxic injury in mice. J. Cell. Sci. 2006;119 (Pt 2): 339-49. doi:10.1242/jcs.02734Pubmed citation

3. Hu K, Lin L, Tan X et-al. tPA protects renal interstitial fibroblasts and myofibroblasts from apoptosis. J. Am. Soc. Nephrol. 2008;19 (3): 503-14. doi:10.1681/ASN.2007030300Free text at pubmedPubmed citation

4. Zhang C, An J, Strickland DK et-al. The low-density lipoprotein receptor-related protein 1 mediates tissue-type plasminogen activator-induced microglial activation in the ischemic brain. Am. J. Pathol. 2009;174 (2): 586-94. doi:10.2353/ajpath.2009.080661Free text at pubmedPubmed citation

5. Shi Y, Mantuano E, Inoue G et-al. Ligand binding to LRP1 transactivates Trk receptors by a Src family kinase-dependent pathway. Sci Signal. 2009;2 (68): ra18. doi:10.1126/scisignal.2000188Free text at pubmedPubmed citation

6. Wu F, Wu J, Nicholson AD et-al. Tissue-type plasminogen activator regulates the neuronal uptake of glucose in the ischemic brain. J. Neurosci. 2012;32 (29): 9848-58. doi:10.1523/JNEUROSCI.1241-12.2012Free text at pubmedPubmed citation

7. Lin, Ling, Yang Jin, and Kebin Hu. ‘Tissue-Type Plasminogen Activator (Tpa) Promotes M1 Macrophage Survival Through P90 Ribosomal S6 Kinase (RSK) And P38 Mitogen-Activated Protein Kinase (MAPK) Pathway’. Journal of Biological Chemistry 290.12 (2015): 7910-7917. Web. 5 June 2015. Link to article

8. Lin L, Jin Y, Mars WM, Reeves WB, Hu K. Myeloid-derived tissue-type plasminogen activator promotes macrophage motility through FAK, Rac1, and NF-κB pathways. Am J Pathol. 2014;184(10):2757-67. Link to article

9. Wu F, Torre E, Cuellar-giraldo D, et al. Tissue-type plasminogen activator triggers the synaptic vesicle cycle in cerebral cortical neurons. J Cereb Blood Flow Metab. 2015. Link to article

10. Su EJ, Fredriksson L, Geyer M, et al. Activation of PDGF-CC by tissue plasminogen activator impairs blood-brain barrier integrity during ischemic stroke. Nat Med. 2008;14(7):731-7. Link to article

11. Jeanneret V, Wu F, Merino P, et al. Tissue-type Plasminogen Activator (tPA) Modulates the Postsynaptic Response of Cerebral Cortical Neurons to the Presynaptic Release of Glutamate. Front Mol Neurosci. 2016;9:121. Link to article.

12. Cooper JM, Rastogi A, Krizo JA, Mintz EM, Prosser RA. Urokinase-type plasminogen activator modulates mammalian circadian clock phase regulation in tissue-type plasminogen activator knockout mice. Eur J Neurosci. 2016; Link to article

13. Lin L, Hu K. Tissue-type plasminogen activator modulates macrophage M2 to M1 phenotypic change through annexin A2-mediated NF-κB pathway. Oncotarget. 2017;8(50):88094-88103. Link to article

14. Mantuano E, Azmoon P, Brifault C, et al. Tissue-type plasminogen activator regulates macrophage activation and innate immunity. Blood. 2017;130(11):1364-1374. Link to article