Non-enzymatic Human tPA

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Product Description

Non-enzymatic Human tPA

This human tPA has an active site serine to alanine mutation which renders it catalytically inactive. The mutation site is S478A on the mature protein and S513A on the complete mRNA sequence (UniProtKB: locus TPA_HUMAN, accession P00750). It is >90percent single chain and retains exosite binding as well as other properties of wild type tPA. Recombinantly produced in insect cells.

Reference:
Identification of a conformationally distinct form of plasminogen
activator inhibitor-1, acting as a noninhibitory substrate for tissue-
type plasminogen activator
PJ Declerck, M De Mol, DE Vaughan, and D Collen
J. Biol. Chem., Jun 1992; 267: 11693 – 11696.

Gene ID: 5327
Swiss-Prot/UniProt ID: P00750

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1. Hurtado M, Lozano JJ, Castellanos E et-al. Activation of the epidermal growth factor signalling pathway by tissue plasminogen activator in pancreas cancer cells. Gut. 2007;56 (9): 1266-74. doi:10.1136/gut.2006.097188Free text at pubmedPubmed citation

2. Lin L, Wu C, Hu K. Tissue plasminogen activator activates NF-κB through a pathway involving annexin A2/CD11b and integrin-linked kinase. J. Am. Soc. Nephrol. 2012;23 (8): 1329-38. doi:10.1681/ASN.2011111123Free text at pubmedPubmed citation

3. Wang J, Zhang X, Mu L et-al. t-PA acts as a cytokine to regulate lymphocyte-endothelium adhesion in experimental autoimmune encephalomyelitis. Clin. Immunol. 2014;152 (1-2): 90-100. doi:10.1016/j.clim.2014.03.004Pubmed citation

4. Lin, Ling, Yang Jin, and Kebin Hu. ‘Tissue-Type Plasminogen Activator (Tpa) Promotes M1 Macrophage Survival Through P90 Ribosomal S6 Kinase (RSK) And P38 Mitogen-Activated Protein Kinase (MAPK) Pathway’. Journal of Biological Chemistry 290.12 (2015): 7910-7917. Web. 5 June 2015. Link to article

5. Bucci JC, Trelle MB, Mcclintock CS, Qureshi T, Jørgensen TJ, Peterson CB. Copper(II) Ions Increase Plasminogen Activator Inhibitor Type 1 Dynamics in Key Structural Regions That Govern Stability. Biochemistry. 2016;55(31):4386-98. LInk to article

6. Mantuano E, Azmoon P, Brifault C, et al. Tissue-type plasminogen activator regulates macrophage activation and innate immunity. Blood. 2017;130(11):1364-1374. Link to article