New Format Test Product Human Immunoglobulin G ELISA Kit


SKU: HUIGGKT-1 Categories: ,

Human Immunoglobulin G ELISA Kit

Format: 96 well 8 removable strip plate
Assay Time: 105 minutes
Sample Types: Serum, Plasma, Hybridoma Cell Supernatants, Ascites
Sample Values: 10.6 mg/ml (Human Citrate Plasma 1:1,000,000 Dilution), 9.2 mg/ml (Human Citrate Plasma 1:2,000,000 Dilution)
Recommended Dilution: 1:1,000,000-1:5,000,000 for normal human plasma
Standard: Purified native human IgG calibrated against the WHO International Standard for Immunoglobulins G, A and M, Human Serum distributed by NIBSC (67/086), South Mimms, Potters Bar, Hertfordshire, UK
Specificity: Total natural IgG. The assay does not distinguish IgG subclasses.
Sensitivity: 0.071 ng/ml
Range: 0.2-200 ng/ml
Species Reactivity: Human, Cyno Monkey (minor)
Negative Reactivity: Mouse, Rat, Rabbit
Intra-assay CV: 4.5%
Inter-assay CV: 5.8%
Recovery: 104%
Linearity: 101%
Minimum Shelf Life: 6 months from manufacture, see label for expiration date
Description: Human Immunoglobulin G (IgG) is the most abundant immunoglobulin in serum and is predominately involved in the secondary immune response. The IgG subclasses are designated 1, 2, 3 and 4 based on their relative prevalence in human serum. Human IgG will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-human IgG primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of human IgG in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified human IgG and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Sample kit instruction sheet
Sample certificate of analysis
Safety Data Sheet

1. Sanyal M, Morimoto M, Baradaran-heravi A, et al. Lack of IL7Rα expression in T cells is a hallmark of T-cell immunodeficiency in Schimke immuno-osseous dysplasia (SIOD). Clin Immunol. 2015; Link to article.

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Human Immunoglobulin G ELISA Kit

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