Mouse uPA total antigen assay ELISA kit

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Mouse uPA total antigen assay ELISA kit

Urokinase plasminogen activator (uPA) is a serine protease that activates plasminogen to plasmin in the blood fibrinolytic system. It is also implicated in events related to cell invasion/migration. The sensitive quantitative measurement of total mouse uPA antigen in plasma and other biological fluid samples is easily performed with this 96 well strip format ELISA kit. The concentration level of uPA antigen in mouse urine has been reported to be 1.8 ug/ml. The assay measures total uPA in the 0.01-10 ng/ml range. Samples giving mouse uPA levels above 10 ng/ml should be diluted in blocking buffer before use. Mouse uPA will bind to the capture antibody coated on the microtiter plate. Free and complexed uPA will be detected by the assay. This kit does not cross react with human uPA. After appropriate washing steps, anti-mouse uPA primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of mouse uPA in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified mouse uPA and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, uPA Antigen Capture Plate, Secondary Antibody

Gene ID: 18792
Swiss-Prot/UniProt ID: P06869

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1. Merchan JR, Tang J, Hu G et-al. Protease activity of urokinase and tumor progression in a syngeneic mammary cancer model. J. Natl. Cancer Inst. 2006;98 (11): 756-64. doi:10.1093/jnci/djj208Pubmed citation

2. Ilkovitch D, Handel-fernandez ME, Herbert LM et-al. Antitumor effects of Mucin 1/sec involves the modulation of urokinase-type plasminogen activator and signal transducer and activator of transcription 1 expression in tumor cells. Cancer Res. 2008;68 (7): 2427-35. doi:10.1158/0008-5472.CAN-07-5651Pubmed citation

3. Ilkovitch D, Lopez DM. Urokinase-mediated recruitment of myeloid-derived suppressor cells and their suppressive mechanisms are blocked by MUC1/sec. Blood. 2009;113 (19): 4729-39. doi:10.1182/blood-2008-08-176438Free text at pubmedPubmed citation

4. Ilkovitch D, Carrio R, Lopez DM. uPA and uPA-receptor are involved in cancer-associated myeloid-derived suppressor cell accumulation. Anticancer Res. 2012;32 (10): 4263-70. Anticancer Res. (link)Pubmed citation

5. Tamura Y, Kawao N, Yano M et-al. Plasminogen activator inhibitor-1 deficiency ameliorates insulin resistance and hyperlipidemia but not bone loss in obese female mice. Endocrinology. 2014;155 (5): 1708-17. doi:10.1210/en.2013-1888Pubmed citation

6. Siefert SA, Chabasse C, Mukhopadhyay S, et al. Enhanced venous thrombus resolution in plasminogen activator inhibitor type-2 deficient mice. J Thromb Haemost. 2014;12(10):1706-16. Link to article