Mix and Go Competent Cells Strain BL21(DE3)
BL21(DE3) strain chemically competent cells from Molecular Innovations are an all purpose reagent for T7 promoter driven IPTG inducible E. coli protein expression. For a transformation efficiency of at least 1×106 simply thaw a tube of cells on ice, add 1-5 ul of DNA, gently mix and incubate on ice for 2-5 minutes, then spread 50-100 ul onto a pre-warmed culture plate. For greater transformation efficiencies heat shock at 37C for 1 minute before plating, or heat shock at 42C for 30 seconds then add SOC media and shake at 225 rpm at 37C for 1 hour before plating. Conveniently supplied as a pack of 5 single use 0.2 ml aliquots.
Genotype: E. coli str. B F– ompT gal dcm lon hsdS20(rB– mB–) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS)
Other strains of competent cells available upon request Click here to request a strain.