Immobilized human plasmin

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We have immobilized human Lys plasmin on agarose resin by coupling of primary amines. Plasmin retains catalytic activity when coupled and may be used to efficiently activate single-chain tPA and uPA to the two-chain form (1). It may also be used to convert plasminogen from its native Glu form to the Lys form by removing the first 77 amino acids. References: 1. Wallen, P. et al. (1982) Biochim. Biophys. Acta 719:318-328.

Gene ID: 5340
Swiss-Prot/UniProt ID: P00747

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1. Florova G, Karandashova S, Declerck PJ et-al. Remarkable stabilization of plasminogen activator inhibitor 1 in a “molecular sandwich” complex. Biochemistry. 2013;52 (27): 4697-709. doi:10.1021/bi400470sFree text at pubmedPubmed citation