Human tPA total antigen assay ELISA kit

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Human tPA total antigen assay ELISA kit

Tissue plasminogen activator (tPA) is a serine protease that catalyzes the activation of plasminogen to plasmin. Clinical studies have indicated that high tPA levels may increase the risk for thrombosis, whereas decreased levels may cause neuronal plasticity and degeneration. The sensitive quantitative measurement of total human tPA antigen in plasma, serum, urine, cell culture media, or tissue extract samples is easily performed with this 96 well strip format ELISA kit. The basal level of tPA in healthy males and females, age 25-34 years, were found to be 5.5 ng/ml and 4.0 ng/ml respectively. The assay measures human tPA in the 0.02-10 ng/ml range. Samples giving human tPA levels above 10 ng/ml should be diluted in blocking buffer before use. Human tPA will bind to the affinity purified capture antibody coated on the microtiter plate. Free and complexed tPA will be detected by the assay. This kit cross reacts with mouse, rat, and pig tPA and does not cross react with rabbit, cyno monkey, rhesus monkey, guinea pig, dog, and sheep tPA. After appropriate washing steps, monoclonal anti-human tPA primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of human tPA in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified human tPA and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, tPA Antigen Capture Plate, Secondary Antibody

Gene ID: 5327
Swiss-Prot/UniProt ID: P00750

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1. Sereda MJ, Bradding P, Vial C. Adenosine potentiates human lung mast cell tissue plasminogen activator activity. J. Immunol. 2011;186 (2): 1209-17. doi:10.4049/jimmunol.1001563Pubmed citation

2. Aloni, Prutha D. et al. ‘Effect Of Fagonia Arabica On Thrombin Induced Release Of T-PA And Complex Of PAI-1 Tpa In Cultured HUVE Cells’. Journal of Traditional and Complementary Medicine (2015): n. pag. Web. 5 June 2015. Link to article

3. Balcı ekmekçi Ö, Ekmekçi H, Güngör Z, et al. Evaluation of Lp-PLA2 mass, vitronectin and PAI-1 activity levels in patients with preeclampsia. Arch Gynecol Obstet. 2015;292(1):53-8. Link to article

4. Fabbro S, Seeds NW. Plasminogen activator activity is inhibited while neuroserpin is up-regulated in the Alzheimer disease brain. J Neurochem. 2009;109(2):303-15. Link to article

5. Chang ML, Lin YS, Pao LH, Huang HC, Chiu CT. Link between plasminogen activator inhibitor-1 and cardiovascular risk in chronic hepatitis C after viral clearance. Sci Rep. 2017;7:42503. Link to article