Human Protein C
Human protein C is prepared from fresh frozen human plasma using a combination of salt precipitations and column chromatography. The protein purity is determined by SDS-PAGE and shows total reduction upon incubation with 2-mercaptoethanol. Protein C is activated to the serine protease Activated Protein C (APC) by alpha-thrombin or the complex of alpha-thrombin/thrombomodulin. APC is a potent anticoagulant through the selective inactivation of Factors Va and VIIIa.