Human PAI-1 NBD labeled at the scissile bond of the reactive loop

$695$1,300

Clear
SKU: HPAI-P1NBD Categories: ,

Product Description

Human PAI-1 NBD labeled at the scissile bond of the reactive loop

P1′-NBD PAI-1 was created by mutagenesis of the methionine residue (Met347) at the P1-P1′ scissile bond to cysteine. This then provides a free thiol group for NBD labeling, a fluorescent probe highly sensitive to changes in solvation and hydrophobic environment. The fluorescence emission of P1′-NBD PAI-1 is quenched upon cleavage of the reactive center loop by a target proteinase.

Gene ID: 5054
Swiss-Prot/UniProt ID: P05121

View sample datasheet
View sample certificate of analysis
View material safety data sheet
Ask a question about this product

Additional Information

Applications
Purity

Source

Molecular Weight

Extinction Coefficient

Species

Storage

Shipping Conditions

Expiration

Form

Buffer

Sample Size

Sample Concentration

Sample Volume

Gene Name

Gene ID

UniProt ID

1. Florova G, Karandashova S, Declerck PJ et-al. Remarkable stabilization of plasminogen activator inhibitor 1 in a “molecular sandwich” complex. Biochemistry. 2013;52 (27): 4697-709. doi:10.1021/bi400470sFree text at pubmedPubmed citation