Human PAI-1 (substrate form – P14 Arginine mutant)
A single substitution at position P14 in the reactive center loop produces a PAI-1 that becomes a substrate for proteinases rather than an inhibitor. Reactive center loop insertion follows protease cleavage and the PAI-1 loses vitronectin binding properties. This molecule is useful for mechanistic studies.
1. Lawrence, D.A. et al. (2000) J. Biol. Chem. 275:5839-5844.
2. Palmieri, D. et al. (2002) J. Biol. Chem. 277:40950-40957.