Human PAI-1 (substrate form – P12 Arginine P14 Arginine double mutant)
Two amino acid substitutions at positions P12 and P14 in the reactive center loop produces a PAI-1 that becomes a substrate for proteinases rather than an inhibitor. The reactive center loop does not insert following protease cleavage and the PAI-1 retains normal vitronectin binding. The double mutation also results in extended half-life compared to native PAI-1. This molecule is useful for mechanistic studies.
1. Huang, Y. et al. (2009) Am. J. Physiol. Renal Physiol. 297:F1045–F1054.
2. Courey, A.J. et al. (2011) Blood. 118:2313–2321.
3. Zhong, J. et al. (2014) Lab. Invest. 94:633–644.