Human Activated Protein C
Activated Protein C (APC) is a serine protease derived from the two chain vitamin K dependent zymogen Protein C. APC inhibits blood coagulation through the selective inactivation of the cofactors Va and VIIIa. APC is prepared from Protein C by activation with purified thrombin. This thrombin is removed after activation by ion exchange chromatography. Activated Protein C purity is determined by SDS-PAGE and shows complete reduction upon incubation with 2-mercaptoethanol.