Prepared from plasminogen by activation with immobilized human uPA. 100 percent functionally active plasmin is purified from the activation reaction by immobilized SBTI. Plasmin will undergo rapid auto proteolysis in the absence of benzamidine and should be used quickly once thawed. Aliquot to avoid freeze-thaw cycles.
Partial cleavage of bovine plasmin between kringles 3 & 4 occurs upon activation, producing a low molecular weight band. This midiplasmin is 100 percent active (Christensen et al. Biochem J. 305 (Pt 1):97-102, 1995).