PAI-1 Knockout Mice

PAI-1 KO mouse model was created by targeted disruption of Serpine1 using programed nuclease. Mutations are confirmed by PCR genotyping and Sanger sequencing. Homozygotes display baseline active PAI-1 level after endotoxin injection. This PAI-1 KO mouse model will be useful for studying fibrinolysis, aging, diabetes and cardiovascular diseases [1-3].

Our mutant mice are from an ongoing colony made in-house at our state of the art facility in the U.S.A and available immediately. No waiting for cryo recovery or costly breeding.

DETAILS

Strain background: C57BL/6J
Chromosome: 5
Gene: Serpine1
Targeted Exon: Exon 2
Mutation: 4 bp deletion on Exon 2

Phenotype:  baseline active PAI-1 level upon endotoxin stimulation

 

 

Table 1 Typical PAI-1 activity in plasma after LPS injection (1.25 mg/kg)

 

 

References:

  1. Yarmolinsky J, Bordin Barbieri N, Weinmann T, Ziegelmann PK, Duncan BB, Schmidt MI. Plasminogen activator inhibitor-1 and type 2 diabetes: a systematic review and meta-analysis of observational studies. Sci Rep. 2016. 6(1):17714.
  2. Song C, Burgess S, Eicher JD, O’Donnell CJ, Johnson AD. Causal Effect of Plasminogen Activator Inhibitor Type 1 on Coronary Heart Disease. J Am Heart Assoc. 2017. 6(6):e004918.
  3. Khan SS, Shah SJ, Klyachko E, Baldridge AS, Eren M, Place AT, et al. A null mutation in SERPINE1 protects against biological aging in humans. Sci Adv. 2017. 3(11):eaao1617.

Click here to order strain C57BL/6J PAI-1 KO mice.

We also provide custom Knockout Mouse Model projects and offer FREE consulting services from our expert scientific staff along with very competitive pricing.

Click here to inquire.

Click here to read our recent research model report.