Protocol for coating wells with vitronectin for cell adhesion
1. Dilute vitronectin to 10 ug/ml in 0.05 M Na Phosphate, 0.15 M NaCl, pH 7.4 (PBS).
2. Coat wells and incubate at 4C overnight.
3. Wash wells three times with PBS.
4. Plate cells.
Did You Find This Article Helpful?
No - 1 visitors found this post was not helpful