High Quality Proteins, Antibodies, and ELISA Kits

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Molecular Innovations is a primary manufacturer of research antibodies, proteins and ELISA kits. We specialize in coagulation and thrombolysis reagents including PAI-1, tPA, urokinase (upa), clotting factors, fibronectin, vitronectin, renin, prorenin, kallikrein, prekallikrein, kininogen, fibrinogen, thrombin, prothrombin, antithrombin, albumin, anhydrotrypsin, trypsin, immunoglobulins, animal tissues, apolipoprotein, c-reactive protein (CRP), cardiac troponin T (CTNT), cathepsin, ceruloplasmin, chromogenic substrates, complement, ecotin, elastase, proteases, reagents, erythropoetin (EPO), factor IX, factor V, factor VII, factor VIII, factor X, factor XI, factor XII, factor XIII, immobilized proteins, interleukins, lipoproteins, receptor associated protein (RAP), neuropserpin, plasma proteins, plasminogen, plasmin, antiplasmin, plasminogen activator inhibitor 1 (PAI-1), platelet proteins, prolactin, prostate specific antigen (PSA), protease nexin 1, protein c, protein S, protein-z-dependent protease inhibitor (ZPI), snake venom proteases, thioester peptide cmk labeling reagents, thrombopoetin, tissue factor, tissue plasminogen activator (TPA), von willebrand factor.

Rabbit anti mouse antithrombin III antiserum

(ASMATIII)

Polyclonal antiserum (host rabbit) to mouse antithrombin.

Rabbit anti mouse antithrombin III IgG fraction

(ASMATIII-GF)

Polyclonal antibody (host rabbit) to mouse antithrombin. IgG fraction purified by immobilized Protein A.

Rabbit anti mouse antithrombin III IgG fraction, biotin labeled

(ASMATIII-GF-BIO)

Biotin labeled polyclonal antibody (host rabbit) to mouse antithrombin. IgG fraction purified by immobilized Protein A.

Rabbit anti mouse antithrombin III IgG fraction, FITC labeled

(ASMATIII-GF-FITC)

FITC labeled polyclonal antibody (host rabbit) to mouse antithrombin. IgG fraction purified by immobilized Protein A.

Rabbit anti mouse antithrombin III IgG fraction, HRP labeled

(ASMATIII-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host rabbit) to mouse antithrombin. IgG fraction purified by immobilized Protein A.

Rabbit anti mouse antithrombin III, affinity purified

(ASMATIII-GF-HT)

Polyclonal antibody (host rabbit). Affinity purified by immobilized mouse antithrombin.

Rabbit anti mouse antithrombin III, biotin labeled affinity purified

(ASMATIII-GF-HT-BIO)

Biotin labeled polyclonal antibody (host rabbit). Affinity purified by immobilized mouse antithrombin.

Sheep anti human CRP antiserum

(SASHCRP)

Polyclonal antiserum (host sheep).

Sheep anti human CRP IgG fraction

(SASHCRP-GF)

Polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti human CRP IgG fraction, biotin labeled

(SASHCRP-GF-BIO)

Biotin labeled polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti human CRP IgG fraction, FITC labeled

(SASHCRP-GF-FITC)

FITC labeled polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti human CRP IgG fraction, HRP labeled

(SASHCRP-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti-human CRP, affinity purified

(SASHCRP-GF-HT)

Polyclonal antibody (host sheep). Affinity purified by immobilized human CRP.

Sheep anti-human CRP, biotin labeled affinity purified

(SASHCRP-GF-HT-BIO)

Biotin labeled polyclonal antibody (host sheep). Affinity purified by immobilized human CRP.

Rat Brain Tissue Lysate

(RT-TL-BR)

A freshly-harvested Sprague Dawley rat brain is flash-frozen in liquid nitrogen within 30 seconds of removal and stored frozen until lysis. The tissue is ground and thoroughly homogenized at 4C with a freshly prepared modified Radio Immuno Precipitation Assay (RIPA) buffer containing strong protease and phosphatase inhibitor cocktails. The final homogenate is incubated with DNase then clarified by centrifugation at 10,000 g at 4C and stored at -80C. Each lot is quality controlled by determining the protein concentration using a BCA assay and analyzed with SDS-PAGE using Coomassie blue for visualization. Sharp, clear protein bands indicate a quality protein lysate.

Rat Heart Tissue Lysate

(RT-TL-HT)

A freshly-harvested Sprague Dawley rat heart is flash-frozen in liquid nitrogen within 30 seconds of removal and stored frozen until lysis. The tissue is ground and thoroughly homogenized at 4C with a freshly prepared modified Radio Immuno Precipitation Assay (RIPA) buffer containing strong protease and phosphatase inhibitor cocktails. The final homogenate is incubated with DNase then clarified by centrifugation at 10,000 g at 4C and stored at -80C. Each lot is quality controlled by determining the protein concentration using a BCA assay and analyzed with SDS-PAGE using Coomassie blue for visualization. Sharp, clear protein bands indicate a quality protein lysate.

Rat Kidney Tissue Lysate

(RT-TL-KD)

A freshly-harvested Sprague Dawley rat kidney is flash-frozen in liquid nitrogen within 30 seconds of removal and stored frozen until lysis. The tissue is ground and thoroughly homogenized at 4C with a freshly prepared modified Radio Immuno Precipitation Assay (RIPA) buffer containing strong protease and phosphatase inhibitor cocktails. The final homogenate is incubated with DNase then clarified by centrifugation at 10,000 g at 4C and stored at -80C. Each lot is quality controlled by determining the protein concentration using a BCA assay and analyzed with SDS-PAGE using Coomassie blue for visualization. Sharp, clear protein bands indicate a quality protein lysate.

Rat Lung Tissue Lysate

(RT-TL-LG)

A freshly-harvested Sprague Dawley rat lung is flash-frozen in liquid nitrogen within 30 seconds of removal and stored frozen until lysis. The tissue is ground and thoroughly homogenized at 4C with a freshly prepared modified Radio Immuno Precipitation Assay (RIPA) buffer containing strong protease and phosphatase inhibitor cocktails. The final homogenate is incubated with DNase then clarified by centrifugation at 10,000 g at 4C and stored at -80C. Each lot is quality controlled by determining the protein concentration using a BCA assay and analyzed with SDS-PAGE using Coomassie blue for visualization. Sharp, clear protein bands indicate a quality protein lysate.

Rat Liver Tissue Lysate

(RT-TL-LR)

A freshly-harvested Sprague Dawley rat liver is flash-frozen in liquid nitrogen within 30 seconds of removal and stored frozen until lysis. The tissue is ground and thoroughly homogenized at 4C with a freshly prepared modified Radio Immuno Precipitation Assay (RIPA) buffer containing strong protease and phosphatase inhibitor cocktails. The final homogenate is incubated with DNase then clarified by centrifugation at 10,000 g at 4C and stored at -80C. Each lot is quality controlled by determining the protein concentration using a BCA assay and analyzed with SDS-PAGE using Coomassie blue for visualization. Sharp, clear protein bands indicate a quality protein lysate.

Rat Brain Lyophilized Powder

(RT-LP-BR)

Sprague Dawley rat brains are frozen, lyophilized, ground and bottled.

Rat Kidney Lyophilized Powder

(RT-LP-KD)

Sprague Dawley rat kidneys are frozen, lyophilized, ground and bottled.

Rat Liver Lyophilized Powder

(RT-LP-LR)

Sprague Dawley rat livers are frozen, lyophilized, ground and bottled.

Sheep anti mouse ZPI antiserum

(SASMZPI)

Polyclonal antiserum (host sheep).

Sheep anti mouse ZPI IgG fraction

(SASMZPI-GF)

Polyclonal antibody (host sheep) to mouse Protein Z-Dependent Protease Inhibitor. IgG fraction purified by immobilized Protein G.

Sheep anti mouse ZPI IgG fraction, biotin labeled

(SASMZPI-GF-BIO)

Biotin labeled polyclonal antibody (host sheep) to mouse Protein Z-Dependent Protease Inhibitor. IgG fraction purified by immobilized Protein G.

Sheep anti mouse ZPI IgG fraction, FITC labeled

(SASMZPI-GF-FITC)

FITC labeled polyclonal antibody (host sheep) to mouse Protein Z-Dependent Protease Inhibitor. IgG fraction purified by immobilized Protein G.

Sheep anti mouse ZPI IgG fraction, HRP labeled

(SASMZPI-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host sheep) to mouse Protein Z-Dependent Protease Inhibitor. IgG fraction purified by immobilized Protein G.

Fluorescein Labeled Phe-Pro-Arg-Chloromethylketone

(PPACK-FITC)

Fluorescein labeled Phe-Pro-Arg-CMK (FPRCK). Extremely potent and selective irreversible inhibitor of thrombin (Kobs/[I] = 107M-1S-1). Reacts with thrombin in a 1:1 stoichiometry. Can also be used to inhibit tissue plasminogen activator, urokinase, Factor VIIa, and Factor XIa.

Fluorescein Labeled Glu-Gly-Arg-Chloromethylketone

(GGACK-FITC)

Fluorescein labeled Glu-Gly-Arg-Chloromethylketone (EGRCK). Potent and specific irreversible inhibitor of Factor Xa. Also known as EGR-chloromethylketone, EGR-CMK, EGRCK, and GGACK.

Sheep anti human tPA, affinity purified

(SASHTPA-GF-HT)

Polyclonal antibody (host sheep). Affinity purified by immobilized human tPA.

Glycosylated Mouse PAI-1 (stable mutant)

(GLYMPAI-I91L)

Glycosylated mouse PAI-1 produced in insect cells with a single conservative mutation (Isoleucine 91 to Leucine) to increase stability.

Albumin, Dog Plasma

(DSA)

Albumin is a water-soluble protein with considerable structural stability which makes up 60percent of the total protein of plasma. It functions as a carrier of hormones, enzymes, fatty acids, metal ions, and medicinal products. Prepared from US origin dog (canine) plasma by proprietary fractionation methods and lyophilized. >95percent pure by SDS-PAGE. Add deionized water or buffer to original or desired volume, aliquot and freeze unused portion.

Equine (horse) plasminogen

(EQPLG)

Prepared from fresh horse plasma by immobilized lysine chromatography. Please inquire about the availability of active horse plasmin.

Rat Cerebral Cortex Tissue Lysate

(RT-TL-CC)

A freshly-harvested Sprague Dawley rat brain is dissected and the cerebral cortex is flash-frozen in liquid nitrogen and stored frozen until lysis. The tissue is ground and thoroughly homogenized at 4C with a freshly prepared modified Radio Immuno Precipitation Assay (RIPA) buffer containing strong protease and phosphatase inhibitor cocktails. The final homogenate is incubated with DNase then clarified by centrifugation at 10,000 g at 4C and stored at -80C. Each lot is quality controlled by determining the protein concentration using a BCA assay and analyzed with SDS-PAGE using Coomassie blue for visualization. Sharp, clear protein bands indicate a quality protein lysate.

Dog plasminogen

(DPLG)

Prepared from fresh US origin dog (canine) plasma by immobilized lysine chromatography.

Cyno Monkey IgG Depleted Serum

(CY-SER-GF)

Prepared from frozen cyno monkey serum using immobilized Protein A.

Biotin-labeled Rabbit anti BSA, affinity purified

(ASBSA-GF-HT-BIO)

Polyclonal antibody (host rabbit). Affinity purified by immobilized BSA. Biotin-labeled.

Rabbit anti human antithrombin III antiserum

(ASHATIII)

Polyclonal antiserum (host rabbit) to human antithrombin.

Rabbit anti human antithrombin III IgG fraction

(ASHATIII-GF)

Polyclonal antibody (host rabbit) to human antithrombin. IgG fraction purified by immobilized Protein A.

Rabbit anti human antithrombin III IgG fraction, biotin labeled

(ASHATIII-GF-BIO)

Biotin labeled polyclonal antibody (host rabbit) to mouse antithrombin. IgG fraction purified by immobilized Protein A.

Rabbit anti human antithrombin III IgG fraction, FITC labeled

(ASHATIII-GF-FITC)

FITC labeled polyclonal antibody (host rabbit) to human antithrombin. IgG fraction purified by immobilized Protein A.

Rabbit anti human antithrombin III IgG fraction, HRP labeled

(ASHATIII-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host rabbit) to human antithrombin. IgG fraction purified by immobilized Protein A.

Rabbit anti human antithrombin III, affinity purified

(ASHATIII-GF-HT)

Polyclonal antibody (host rabbit). Affinity purified by immobilized human antithrombin.

Rabbit anti human prothrombin antiserum

(ASHPT)

Polyclonal antiserum (host rabbit) to human prothrombin and thrombin.

Rabbit anti human prothrombin IgG fraction

(ASHPT-GF)

Polyclonal antibody (host rabbit) to human prothrombin and thrombin. IgG fraction purified by immobilized Protein A.

Rabbit anti human prothrombin IgG fraction, biotin labeled

(ASHPT-GF-BIO)

Biotin labeled polyclonal antibody (host rabbit) to human prothrombin and thrombin. IgG fraction purified by immobilized Protein A.

Rabbit anti human prothrombin IgG fraction, FITC labeled

(ASHPT-GF-FITC)

FITC labeled polyclonal antibody (host rabbit) to human prothrombin and thrombin. IgG fraction purified by immobilized Protein A.

Rabbit anti human prothrombin IgG fraction, HRP labeled

(ASHPT-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host rabbit) to human prothrombin and thrombin. IgG fraction purified by immobilized Protein A.

Rabbit anti human prothrombin IgG fraction, affinity purified

(ASHPT-GF-HT)

Polyclonal antibody (host rabbit). Affinity purified by immobilized human prothrombin.

Rabbit anti human fibronectin IgG fraction

(ASHFBN-GF)

Polyclonal antibody (host rabbit) to human fibronectin. IgG fraction purified by immobilized Protein A.

Rabbit anti human fibronectin IgG fraction, biotin labeled

(ASHFBN-GF-BIO)

Biotin labeled polyclonal antibody (host rabbit) to human fibronectin. IgG fraction purified by immobilized Protein A.

Rabbit anti human fibronectin IgG fraction, FITC labeled

(ASHFBN-GF-FITC)

FITC labeled polyclonal antibody (host rabbit) to human fibronectin. IgG fraction purified by immobilized Protein A.

Rabbit anti human fibronectin IgG fraction, HRP labeled

(ASHFBN-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host rabbit) to human fibronectin. IgG fraction purified by immobilized Protein A.

Rabbit anti human fibronectin, affinity purified

(ASHFBN-GF-HT)

Polyclonal antibody (host rabbit). Affinity purified by immobilized human fibronectin.

Rabbit anti human fibronectin antiserum

(ASHFBN)

Polyclonal antiserum (host rabbit) to human fibronectin.

Rabbit anti mouse fibronectin antiserum

(ASMFBN)

Polyclonal antiserum (host rabbit) to mouse fibronectin.

Rabbit anti mouse fibronectin IgG fraction

(ASMFBN-GF)

Polyclonal antibody (host rabbit) to mouse fibronectin. IgG fraction purified by immobilized Protein A.

Rabbit anti mouse fibronectin IgG fraction, biotin labeled

(ASMFBN-GF-BIO)

Biotin labeled polyclonal antibody (host rabbit) to mouse fibronectin. IgG fraction purified by immobilized Protein A.

Rabbit anti mouse fibronectin IgG fraction, FITC labeled

(ASMFBN-GF-FITC)

FITC labeled polyclonal antibody (host rabbit) to mouse fibronectin. IgG fraction purified by immobilized Protein A.

Rabbit anti mouse fibronectin IgG fraction, HRP labeled

(ASMFBN-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host rabbit) to mouse fibronectin. IgG fraction purified by immobilized Protein A.

Rabbit anti mouse fibronectin, affinity purified

(ASMFBN-GF-HT)

Polyclonal antibody (host rabbit). Affinity purified by immobilized mouse fibronectin.

Rabbit anti mouse prorenin/renin antiserum

(ASMPREN)

Polyclonal antiserum (host rabbit) to mouse prorenin.

Rabbit anti mouse prorenin/renin IgG fraction

(ASMPREN-GF)

Polyclonal antibody (host rabbit) to mouse prorenin. IgG fraction purified by immobilized Protein A.

Rabbit anti mouse prorenin/renin IgG fraction, biotin labeled

(ASMPREN-GF-BIO)

Biotin labeled polyclonal antibody (host rabbit) to mouse prorenin. IgG fraction purified by immobilized Protein A.

Rabbit anti mouse prorenin/renin IgG fraction, FITC labeled

(ASMPREN-GF-FITC)

FITC labeled polyclonal antibody (host rabbit) to mouse prorenin. IgG fraction purified by immobilized Protein A.

Rabbit anti mouse prorenin/renin IgG fraction, HRP labeled

(ASMPREN-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host rabbit) to mouse prorenin. IgG fraction purified by immobilized Protein A.

Rabbit Immunoglobulin G ELISA Kit

(RBIGGKT)

Immunoglobulin G (IgG) is the most abundant immunoglobulin in serum and is predominately involved in the secondary immune response. The IgG subclasses are designated 1, 2, 3 and 4 based on their relative prevalence in serum. The sensitive quantitative measurement of total rabbit IgG antigen in serum, plasma, hybridoma cell supernatants, ascites or other biological fluid samples is easily performed with this 96 well strip format ELISA kit. The assay does not distinguish IgG subclasses. The kit does not cross react with human, pig, rat, dog, sheep or mouse. It cross reacts with guinea pig at 0.002 percent. The concentration of IgG in normal rabbit serum ranges from 5 to 12 mg/ml. The assay measures rabbit IgG in the 0.1-100 ng/ml range. Samples giving rabbit IgG levels above 100 ng/ml should be diluted in blocking buffer before use. A 1:1,000,000 to 1:10,000,000 dilution for serum is suggested for best results. Rabbit IgG will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, peroxidase labeled anti-rabbit IgG primary antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of rabbit IgG in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified rabbit IgG and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Cathepsin S, Human Spleen

(HCS)

Like Cathepsins B, H, and L, Cathepsin S belongs to the group of lysosomal cysteine proteases. Cathepsin S is found primarily in the spleen and in lung macrophages. Its level is elevated in the brain tissue of those with Alzheimer’s disease and Down syndrome. Cathepsin S may function in the processing of amyloid precursor protein to amyloid beta peptides. Cathepsin S activity is detected using Z-Val-Val-Arg-AMC (Bachem: I-1540). Prepared from tissue shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE.

Bovine IgG, Protein G Purified

(BV-GF)

Purified from normal serum by immobilized Protein G. >95 percent pure by SDS-PAGE and preservative free.

Anti Human Thrombin Antithrombin Complex

(TAT1F7F8)

Mouse monoclonal antibody to human thrombin antithrombin III complex. Reacts with thrombin antithrombin complex but not thrombin or antithrombin under non-reducing conditions. IgG fraction purified by immobilized Protein G.

Mouse Immunoglobulin G ELISA Kit

(MSIGGKT)

Immunoglobulin G (IgG) is the most abundant immunoglobulin in serum and is predominately involved in the secondary immune response. The IgG subclasses are designated 1, 2, 3 and 4 based on their relative prevalence in serum. The sensitive quantitative measurement of total mouse IgG antigen in serum, plasma, hybridoma cell supernatants, ascites or other biological fluid samples is easily performed with this 96 well strip format ELISA kit. The assay does not distinguish IgG subclasses. The kit does not cross react significantly with rat, human, guinea pig, or rabbit IgG. The concentration of IgG in normal mouse serum ranges from 5 to 12 mg/ml. The assay measures mouse IgG in the 0.1-100 ng/ml range. Samples giving mouse IgG levels above 100 ng/ml should be diluted in blocking buffer before use. A 1:1,000,000 to 1:20,000,000 dilution for serum is suggested for best results. Mouse IgG will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, peroxidase labeled anti-mouse IgG primary antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of mouse IgG in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified mouse IgG and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Human Factor IX total antigen assay ELISA kit

(HFIXKT-TOT)

Factor IX (aka Christmas Factor) is a single-chain, 415 amino acid glycoprotein zymogen. Factor IX is activated by either Factor XIa or the Factor VIIa complex. Factor IXa converts Factor X to Factor Xa during the intrinsic pathway of the coagulation cascade. Factor IX is used to treat patients with hemophilia B, an X-linked bleeding disorder. The sensitive quantitative measurement of total human Factor IX antigen in plasma samples is easily performed with this 96 well strip format ELISA kit. Factor IX and IXa will be detected by the assay. The concentration of Factor IX in normal human plasma is about 5 ug/ml. The assay measures total human Factor IX in the 0.1-100 ng/ml range. Samples giving human Factor IX levels above 100 ng/m should be diluted in blocking buffer before use. A 1:500 to 1:1,000 dilution for plasma is suggested for best results. Human Factor IX will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, anti-human Factor IX primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of Factor IX in the samples. A standard calibration curve is prepared using dilutions of purified Factor IX and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

The Human Factor IX standard provided in this kit is calibrated against the WHO International Standard for Human Plasma Factors II, VII, IX, X (NIBSC Code 09/172).

Mouse PAI-1 (Biotin labeled active fraction)

(MPAI-BIO)

This active fraction of recombinant wild type mouse PAI-1 is biotin labeled on lysine residues. Typical preparations are ~50 percent active.

Cyno Albumin

(CYSA)

Cyno albumin is prepared from cynomolgus (cyno) monkey plasma by proprietary fractionation methods and lyophilized. Albumin is a water-soluble protein with considerable structural stability which makes up 60percent of the total protein of plasma. It functions as a carrier of hormones, enzymes, fatty acids, metal ions, and medicinal products. >95percent pure by SDS-PAGE. Add deionized water or buffer to original or desired volume, aliquot and freeze unused portion.

Human Factor XII total antigen assay ELISA kit

(HFXIIKT-TOT)

Factor XII (aka Hageman Factor) is a single-chain, 615 amino acid glycoprotein zymogen. Factor XII is activated by kallikrein. Factor XIIa converts prekallikrein to kallikrein during the intrinsic pathway of the coagulation cascade. Although Factor XII is not thought to play an essential role in normal hemostasis, lack of Factor XII in a mouse model resulted in a severe defect in thrombus formation. The sensitive quantitative measurement of total human Factor XII antigen in plasma samples is easily performed with this 96 well strip format ELISA kit. Factor XII and XIIa will be detected by the assay. The concentration of Factor XII in normal human plasma ranges from 15-47 ug/ml. The assay measures total human Factor XII in the 0.1-100 ng/ml range. Samples giving human Factor XII levels above 100 ng/ml should be diluted in blocking buffer before use. A 1:1,000 to 1:5,000 dilution for plasma is suggested for best results. Human Factor XII will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, anti-human Factor XII primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of Factor XII in the samples. A standard calibration curve is prepared using dilutions of purified Factor XII and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Albumin, Rat Plasma

(RSA)

Albumin is a water-soluble protein with considerable structural stability which makes up 60percent of the total protein of plasma. It functions as a carrier of hormones, enzymes, fatty acids, metal ions, and medicinal products. Prepared from Sprague Dawley rat plasma by proprietary fractionation methods and lyophilized. >95percent pure by SDS-PAGE. Add deionized water or buffer to original or desired volume, aliquot and freeze unused portion.

Mouse Cerebral Cortex Tissue Lysate

(MS-TL-CC)

A freshly-harvested Balb C mouse brain is dissected and the cerebral cortex is flash-frozen in liquid nitrogen and stored frozen until lysis. The tissue is ground and thoroughly homogenized at 4C with a freshly prepared modified Radio Immuno Precipitation Assay (RIPA) buffer containing strong protease and phosphatase inhibitor cocktails. The final homogenate is incubated with DNase then clarified by centrifugation at 10,000 g at 4C and stored at -80C. Each lot is quality controlled by determining the protein concentration using a BCA assay and analyzed with SDS-PAGE using Coomassie blue for visualization. Sharp, clear protein bands indicate a quality protein lysate.

Mouse Cerebellum Tissue Lysate

(MS-TL-CB)

A freshly-harvested Balb C mouse brain is dissected and the cerebellum is flash-frozen in liquid nitrogen and stored frozen until lysis. The tissue is ground and thoroughly homogenized at 4C with a freshly prepared modified Radio Immuno Precipitation Assay (RIPA) buffer containing strong protease and phosphatase inhibitor cocktails. The final homogenate is incubated with DNase then clarified by centrifugation at 10,000 g at 4C and stored at -80C. Each lot is quality controlled by determining the protein concentration using a BCA assay and analyzed with SDS-PAGE using Coomassie blue for visualization. Sharp, clear protein bands indicate a quality protein lysate.

Mouse Kidney Tissue Lysate

(MS-TL-KD)

A freshly-harvested Balb C mouse kidney is flash-frozen in liquid nitrogen within 30 seconds of removal and stored frozen until lysis. The tissue is ground and thoroughly homogenized at 4C with a freshly prepared modified Radio Immuno Precipitation Assay (RIPA) buffer containing strong protease and phosphatase inhibitor cocktails. The final homogenate is incubated with DNase then clarified by centrifugation at 10,000 g at 4C and stored at -80C. Each lot is quality controlled by determining the protein concentration using a BCA assay and analyzed with SDS-PAGE using Coomassie blue for visualization. Sharp, clear protein bands indicate a quality protein lysate.

Human Prolactin ELISA Kit

(HPRLKT)

Prolactin (PRL) is a 199 aa, 23kD peptide hormone that is secreted primarily by the pituitary gland in both males and females, though its major roles are in pregnancy and lactation. Prolactin may have a role in breast cancer development, with higher prolactin levels correlating with postmenopausal breast cancer risk. The sensitive quantitative measurement of total human prolactin antigen in plasma, serum, or breast milk samples is easily performed with this 96 well strip format ELISA kit. The concentration of prolactin in normal human plasma ranges from 2-18 ng/ml in males, 2-29 ng/ml in nonpregnant females, and 10-209 ng/ml in pregnant females. The assay measures total human prolactin in the 0.1-100 ng/ml range. Samples with human prolactin levels above 100ng/ml should be diluted in blocking buffer before use. Normal plasma should not require dilution before use in this assay. A 1:2 to 1:4 dilution for breast milk is suggested for best results. Human prolactin will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-human prolactin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of prolactin in the samples. A standard calibration curve is prepared using dilutions of purified prolactin and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Mouse Prothrombin ELISA kit

(MPTKT)

Prothrombin is a single-chain vitamin K dependent 579 amino acid glycoprotein zymogen. Prothrombin levels are decreased by anticoagulant therapy, vitamin K deficiency and severe liver disease. Elevated plasma prothrombin is associated with a single nucleotide change at position 20210. The sensitive quantitative measurement of mouse prothrombin in plasma samples is easily performed with this 96 well strip format ELISA kit. Thrombin and thrombin-antithrombin complex will not be detected by the assay. Prothrombin in normal human plasma ranges from 110-212 ug/ml with an average concentration of 150 ug/ml. Normal values of prothrombin in mouse plasma have not been conclusively determined but are believed to be similar to human plasma. The assay measures mouse prothrombin in the 1-500 ng/ml range. Samples giving mouse prothrombin levels above 500 ng/ml should be diluted in blocking buffer before use. A 1:10,000 dilution for plasma is suggested for best results. Mouse prothrombin will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-mouse prothrombin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with streptavidin conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of prothrombin in the samples. A standard calibration curve is prepared using dilutions of purified prothrombin and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

This assay uses a unique antibody to only detect prothrombin. A total antigen kit that will detect prothrombin, thrombin and TAT complex is available under catalog number MPTKT-TOT.

Human Prolactin

(HPRL)

Prolactin (PRL, Mammotropin, Luterotropic hormone, Lutetropin) is a neuroendocrine peptide hormone that is secreted primarily by the pituitary gland. Expressed recombinantly and purified by chromatography. >95percent pure by SDS-PAGE and biologically active. Add deionized water to original volume, aliquot and freeze unused portion.

Mouse Prolactin

(MPRL)

Prolactin (PRL, Mammotropin, Luterotropic hormone, Lutetropin) is a neuroendocrine peptide hormone that is secreted primarily by the pituitary gland. Expressed recombinantly and purified by chromatography. >95percent pure by SDS-PAGE and biologically active. Add deionized water to original volume, aliquot and freeze unused portion.

Rat Prolactin

(RPRL)

Prolactin (PRL, Mammotropin, Luterotropic hormone, Lutetropin) is a neuroendocrine peptide hormone that is secreted primarily by the pituitary gland. Expressed recombinantly and purified by chromatography. >95percent pure by SDS-PAGE and biologically active. Add deionized water to original volume, aliquot and freeze unused portion.

Human Prolactin Receptor

(HPRLR)

Prolactin is a neuroendocrine peptide hormone that is secreted primarily by the pituitary gland. Prolactin receptor is a class 1 cytokine transmembrane receptor that varies in size with tissue source and species. It consists of an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region. The extracellular domain is expressed recombinantly and purified by chromatography. >95percent pure by SDS-PAGE and biologically active. Add deionized water to original volume, aliquot and freeze unused portion.

Rat Prolactin Receptor

(RPRLR)

Prolactin is a neuroendocrine peptide hormone that is secreted primarily by the pituitary gland. Prolactin receptor is a class 1 cytokine transmembrane receptor that varies in size with tissue source and species. It consists of an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region. The extracellular domain is expressed recombinantly and purified by chromatography. >95percent pure by SDS-PAGE and biologically active. Add deionized water to original volume, aliquot and freeze unused portion.

Rabbit fibrinogen total antigen assay ELISA kit

(RBFBGNKT)

Fibrinogen is a soluble glycoprotein that circulates in the blood and is converted to insoluble fibrin by thrombin in the final step of the coagulation cascade. Hepatic expression of fibrinogen increases two to four hundred fold during the acute phase response to infection or inflammation. Elevated fibrinogen levels are correlated with cardiovascular disease and atherosclerosis. The sensitive quantitative measurement of rabbit fibrinogen in plasma and serum samples is easily performed with this 96 well strip format ELISA kit. Fibrinogen is present in normal rabbit plasma at a concentration of 3.07 mg/ml. The assay measures total rabbit fibrinogen in the 0.5-500 ng/ml range. Samples giving rabbit fibrinogen levels above 500 ng/ml should be diluted in 1X diluent before use. A 1:500,000 to 1:1,000,000 dilution for plasma or serum is suggested for best results. Rabbit fibrinogen will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-rabbit fibrinogen primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of fibrinogen in the samples. A standard calibration curve is prepared using dilutions of purified fibrinogen and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate

Swine IgG, Protein G Purified

(SW-GF)

Purified from normal serum by immobilized Protein G. >95 percent pure by SDS-PAGE and preservative free.

Mouse Prolactin ELISA Kit

(MPRLKT)

Prolactin (PRL) is a 199 aa, 23kD peptide hormone that is secreted primarily by the pituitary gland in both males and females, though its major roles are in pregnancy and lactation. Prolactin may have a role in breast cancer development, with higher prolactin levels correlating with postmenopausal breast cancer risk. The sensitive quantitative measurement of total mouse prolactin antigen in plasma is easily performed with this 96 well strip format ELISA kit. The concentration of prolactin in pooled normal mouse plasma determined by in house testing was 4.7 ng/ml. Prolactin levels are elevated in pregnant mice and peak at day 8 of pregnancy. The assay measures total mouse prolactin in the 0.1-100 ng/ml range. Samples with mouse prolactin levels above 100 ng/ml should be diluted in blocking buffer before use. Normal plasma may be applied directly to the plate or diluted 1:1 with blocking buffer. Mouse prolactin will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-mouse prolactin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of prolactin in the samples. A standard calibration curve is prepared using dilutions of purified prolactin and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Rat Prolactin ELISA Kit

(RPRLKT)

Prolactin (PRL) is a 199 aa, 23kD peptide hormone that is secreted primarily by the pituitary gland in both males and females, though its major roles are in pregnancy and lactation. Prolactin may have a role in breast cancer development, with higher prolactin levels correlating with postmenopausal breast cancer risk. The sensitive quantitative measurement of total rat prolactin antigen in plasma is easily performed with this 96 well strip format ELISA kit. The concentration of prolactin in pooled normal rat plasma determined by in house testing was 6.8 ng/ml. Prolactin levels in pregnant rats are elevated immediately before birth. The assay measures total rat prolactin in the 0.1-100 ng/ml range. Samples with rat prolactin levels above 100 ng/ml should be diluted in blocking buffer before use. Normal plasma may be applied directly to the plate or diluted 1:1 with blocking buffer. Rat prolactin will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-rat prolactin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of prolactin in the samples. A standard calibration curve is prepared using dilutions of purified prolactin and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Anti Human Factor V, Clone 12C5

(FV12C5)

Mouse monoclonal antibody to human Factor V. IgG fraction purified by immobilized Protein G. Isotype IgG1Kappa.

Anti Human Factor V, Biotin Labeled Clone 12C5

(FV12C5-BIO)

Biotin labeled mouse monoclonal antibody to human Factor V. IgG fraction purified by immobilized Protein G. Clone 12C5, isotype IgG1Kappa.

Rabbit anti mouse prorenin/renin IgG fraction, High Titer

(ASMPREN-GF-HT)

Polyclonal antibody (host rabbit) to mouse prorenin. Affinity purified IgG fraction.

Biotin labeled Rabbit anti mouse prorenin/renin IgG fraction, High Titer

(ASMPREN-GF-HT-BIO)

Biotin labeled polyclonal antibody (host rabbit) to mouse prorenin. Affinity purified IgG fraction.

Human PAI-1 (wild type active fraction – N-terminal poly-histidine tag)

(6X-HISHPAI-A)

The N-terminus of this wild type human PAI-1 has been modified with the introduction of a 6-X histidine tag. The tag allows for the immobilization of functionally active PAI-1 onto surfaces such as metal chelate microtiter plates or Ni+2 resins. The purification conditions are gentle and result in an active fraction >99percent pure and >98percent active as determined by SDS PAGE.

Mouse IL-2, chiMAX Fc Fusion Protein

(MIL2-FC)

Interleukin-2 (IL-2) is a O-glycosylated four alpha-helix bundle cytokine that is produced by T-cells in response to antigenic or mitogenic stimulation. IL-2 can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells and is required for T-cell proliferation and other activities crucial to regulation of the immune response. Mouse IL-2 (Ala21-Gln169) is expressed recombinantly, purified by chromatography, sterile filtered and lyophilized. >95percent pure by SDS-PAGE and biologically active as measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T-cells. (Gearing and Bird (1987) Production and assay of interleukin 2. In Lymphokines and Interferons: A Practical Approach (Clemens et al. eds.) pp. 291-301). The ED50 for this effect is typically < 1.0 ng/ml. Add deionized water to original volume, aliquot and freeze unused portion.

This chiMAX Fc Fusion Protein is glycosylated and expressed as a chimera with the Fc region of mouse immunoglobulin (heavy chain hinge, CH2 and CH3). Fc fusion proteins are typically more stable and resistant to degradation and clearance than native cytokines. Fc fusion proteins appear as a dimer in SDS-PAGE under non-reducing conditions.

50% off your first order! Please reference promotion code MP2103. Offer expires April 30, 2013.

Anti Human Factor XI, Clone 2B11

(FXI2B11)

Mouse monoclonal antibody to Human Factor XI. IgG fraction purified by immobilized Protein G. Detects Factor XI and Factor XIa under non-reducing and reducing conditions. Epitope localized to the heavy chain. Clone 2B11-E5F6, isotype IgG1 Kappa.

Click here to compare all our monoclonal antibodies to human Factor XI

Anti Human Factor XI, Clone 9C9E10

(FXI9C9E10)

Mouse monoclonal antibody to Human Factor XI. IgG fraction purified by immobilized Protein G. Detects Factor XI and Factor XIa under non-reducing conditions. Epitope localized to the heavy chain. Clone 9C9-E10F7, isotype IgG1 Kappa.

Click here to compare all our monoclonal antibodies to human Factor XI

Anti Human Factor XI, Clone 14F8

(FXI14F8)

Mouse monoclonal antibody to Human Factor XI. IgG fraction purified by immobilized Protein G. Detects Factor XI and Factor XIa under non-reducing and reducing conditions. Epitope localized to the light chain. Clone 14F8-F10, isotype IgG1 Kappa.

Click here to compare all our monoclonal antibodies to human Factor XI

Anti Human Factor XII, Clone 20B2

(FXII20B2)

Mouse monoclonal antibody to Human Factor XII. IgG fraction purified by immobilized Protein G. Detects Factor XII and Factor XIIa under non-reducing and reducing conditions. Epitope localized to the heavy chain. Clone 20B2-5B5, isotype IgG1 Kappa.

Anti Human Factor XII, Clone 11B9

(FXII11B9)

Mouse monoclonal antibody to Human Factor XII. IgG fraction purified by immobilized Protein G. Detects Factor XII, Factor XIIa and Factor beta-XIIa under non-reducing conditions. Epitope localized to the light chain. Clone 11B12-4F8, isotype IgG1 Kappa.

Rat PAI-1 (Alexa Fluor 488 labeled wild type active form)

(RPAI-AF488)

Recombinant rat PAI-1 is labeled with Alexa Fluor 488 at primary amines (Abs: 495, Em: 519). This wild type fraction typically contains 70percent active PAI-1.

Human Prolactin Receptor, chiMAX Fc Fusion Protein

(HPRLR-FC)

Prolactin is a neuroendocrine peptide hormone that is secreted primarily by the pituitary gland. Prolactin receptor is a class 1 cytokine transmembrane receptor that varies in size with tissue source and species. It consists of an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region. The extracellular domain (Gln25-Asp234) is expressed recombinantly, purified by chromatography, sterile filtered and lyophilized. >95percent pure by SDS-PAGE and biologically active as measured by inhibitory activity toward prolactin-induced proliferation of Nb2-11 rat lymphoma cells (Gout et al. (1980) Cancer Res. 40:2433). ED50 for this effect is typically < 1.0 ug/ml in the presence of 0.5 ng/ml of recombinant human Prolactin. Add deionized water to original volume, aliquot and freeze unused portion.

This chiMAX Fc Fusion Protein is glycosylated and expressed as a chimera with the Fc region of mouse immunoglobulin (heavy chain hinge, CH2 and CH3). Fc fusion proteins are typically more stable and resistant to degradation and clearance than native cytokines. Fc fusion proteins appear as a dimer in SDS-PAGE under non-reducing conditions.

50% off your first order! Please reference promotion code MP2103. Offer expires April 30, 2013.

Anti Human Factor V, Clone 14G5

(FV14G5)

Mouse monoclonal antibody to human Factor V. IgG fraction purified by immobilized Protein G. Clone 14G5-G11, isotype IgG1Kappa.

Anti Human Prekallikrein, Clone 10G3

(PK10G3)

Mouse monoclonal antibody to human prekallikrein. Excellent reagent for affinity purification of prekallikrein from human plasma. IgG fraction purified by immobilized Protein G. Clone 10G3-42, isotype IgG1 kappa. Also available immobilized to agarose resin.

Anti Human Factor V, Biotin Labeled Clone 14G5

(FV14G5-BIO)

Biotin labeled mouse monoclonal antibody to human Factor V. IgG fraction purified by immobilized Protein G. Clone 14G5-G11, isotype IgG1Kappa.

Sheep anti human PSA antiserum

(SASHPSA)

Polyclonal antiserum (host rabbit) to human PSA.

Sheep anti human PSA IgG fraction

(SASHPSA-GF)

Polyclonal antibody (host sheep) to human PSA. IgG fraction purified by immobilized Protein G.

50% off your first order! Please reference promotion code MP2103. Offer expires April 30, 2013.

Sheep anti human PSA IgG fraction, biotin labeled

(SASHPSA-GF-BIO)

Biotin labeled polyclonal antibody (host sheep) to human PSA. IgG fraction purified by immobilized Protein G.

50% off your first order! Please reference promotion code MP2103. Offer expires April 30, 2013.

Sheep anti human PSA IgG fraction, FITC labeled

(SASHPSA-GF-FITC)

FITC labeled polyclonal antibody (host sheep) to human PSA. IgG fraction purified by immobilized Protein G.

50% off your first order! Please reference promotion code MP2103. Offer expires April 30, 2013.

Sheep anti human PSA IgG fraction, HRP labeled

(SASHPSA-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host sheep) to human PSA. IgG fraction purified by immobilized Protein G.

50% off your first order! Please reference promotion code MP2103. Offer expires April 30, 2013.

Sheep anti human cTnT antiserum

(SASHCTNT)

Polyclonal antiserum (host rabbit) to human Cardiac Troponin T (cTnT).

50% off your first order! Please reference promotion code MP2103. Offer expires April 30, 2013.

Sheep anti human cTnT IgG fraction

(SASHCTNT-GF)

Polyclonal antibody (host sheep) to human Cardiac Troponin T (cTnT). IgG fraction purified by immobilized Protein G.

50% off your first order! Please reference promotion code MP2103. Offer expires April 30, 2013.

Sheep anti human cTnT IgG fraction, biotin labeled

(SASHCTNT-GF-BIO)

Biotin labeled polyclonal antibody (host sheep) to human Cardiac Troponin T (cTnT). IgG fraction purified by immobilized Protein G.

50% off your first order! Please reference promotion code MP2103. Offer expires April 30, 2013.

Sheep anti human cTnT IgG fraction, FITC labeled

(SASHCTNT-GF-FITC)

FITC labeled polyclonal antibody (host sheep) to human Cardiac Troponin T (cTnT). IgG fraction purified by immobilized Protein G.

50% off your first order! Please reference promotion code MP2103. Offer expires April 30, 2013.

Sheep anti human cTnT IgG fraction, HRP labeled

(SASHCTNT-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host sheep) to human Cardiac Troponin T (cTnT). IgG fraction purified by immobilized Protein G.

50% off your first order! Please reference promotion code MP2103. Offer expires April 30, 2013.

Anti Human PSA, Clone 14E7

(PSA14E7)

Mouse monoclonal antibody to human PSA. IgG fraction purified by immobilized Protein G. Detects Prostate Specific Antigen under non-reducing conditions. Clone 14E7-E5, isotype IgG1 Kappa.

50% off your first order! Please reference promotion code MP2103. Offer expires April 30, 2013.

Anti Human PSA, Clone 16G3

(PSA16G3)

Mouse monoclonal antibody to human PSA. IgG fraction purified by immobilized Protein G. Detects Prostate Specific Antigen under non-reducing conditions. Clone 16G3-C7E10, isotype IgG1 Kappa.

50% off your first order! Please reference promotion code MP2103. Offer expires April 30, 2013.

Anti Human PSA, Clone 18D4

(PSA18D4)

Mouse monoclonal antibody to human PSA. IgG fraction purified by immobilized Protein G. Detects Prostate Specific Antigen under non-reducing conditions. Clone 18D4-B5D4, isotype IgG2b.

50% off your first order! Please reference promotion code MP2103. Offer expires April 30, 2013.

Anti Human PSA, Clone 18D11

(PSA18D11)

Mouse monoclonal antibody to human PSA. IgG fraction purified by immobilized Protein G. Detects Prostate Specific Antigen under non-reducing conditions. Clone 18D11-E9B4, isotype IgG2b.

50% off your first order! Please reference promotion code MP2103. Offer expires April 30, 2013.

Anti Human cTnT, Clone 3B10

(CTNT3B10)

Mouse monoclonal antibody to human Cardiac Troponin T (cTnT). IgG fraction purified by immobilized Protein G. Detects cTnT in western blot under non-reducing and reducing conditions. Cross reacts with human Skeletal Troponin T (sTNT, TNNT1). Clone 3B10-G11, isotype IgG1 Kappa.

50% off your first order! Please reference promotion code MP2103. Offer expires April 30, 2013.

Rat Renin Substrate Plasma, Male, Sodium EDTA

(RPLA-EDTA-ANG-M)

In the renin-angiotensin system (RAS), renin activates angiotensinogen to yield angiotensin I, which is further converted into angiotensin II by angiotensin-converting enzyme. Removal of the kidney generates renin/prorenin deficient plasma with high levels of angiotensinogen. This nephrectomized rat plasma can be used as a source of angiotensinogen or as a substrate for renin generation of Ang I. Plasma is collected from male rats at least 48 hours post nephrectomy. The substrate activity is determined by incubation with renin followed by Ang I radio-immune assay (RIA).

Also available:
Rat Prorenin/Renin Total Antigen Assay (RPRENKT-TOT)
Rat Prorenin (RPREN-HIS)
Polyclonal Antibodies ( Click here for list)
Rat Renin/Prorenin Double Depleted Plasma (RPLA-SC-PREN)
Rat Renin Substrate Plasma, Female, Sodium EDTA (RPLA-EDTA-ANG-F)

Rat Renin Substrate Plasma, Female, Sodium EDTA

(RPLA-EDTA-ANG-F)

In the renin-angiotensin system (RAS), renin activates angiotensinogen to yield angiotensin I, which is further converted into angiotensin II by angiotensin-converting enzyme. Removal of the kidney generates renin/prorenin deficient plasma with high levels of angiotensinogen. This nephrectomized rat plasma can be used as a source of angiotensinogen or as a substrate for renin generation of Ang I. Plasma is collected from female rats at least 48 hours post nephrectomy. The substrate activity is determined by incubation with renin followed by Ang I radio-immune assay (RIA).

Anti Human CRP, Clone 16B2

(CRP16B2)

Mouse monoclonal antibody to human C-Reactive Protein (CRP). IgG fraction purified by immobilized Protein G. Detects CRP under non-reducing and reducing conditions. Clone 16B2-3G5, isotype IgG1 Kappa.

Porcine fibrinogen total antigen assay ELISA kit

(PFBGNKT)

Fibrinogen is a soluble glycoprotein that circulates in the blood and is converted to insoluble fibrin by thrombin in the final step of the coagulation cascade. Hepatic expression of fibrinogen increases two to four hundred fold during the acute phase response to infection or inflammation. Elevated fibrinogen levels are correlated with cardiovascular disease and atherosclerosis. The sensitive quantitative measurement of porcine fibrinogen (pig fibrinogen) in plasma and serum samples is easily performed with this 96 well strip format ELISA kit. Fibrinogen is present in normal porcine plasma at a concentration of 2-4 mg/ml. The assay measures total porcine fibrinogen in the 1.56-800 ng/ml range. Samples giving porcine fibrinogen levels above 800 ng/ml should be diluted in 1X diluent before use. A 1:500,000 to 1:1,000,000 dilution for plasma or serum is suggested for best results. Porcine fibrinogen will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-porcine fibrinogen primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of fibrinogen in the samples. A standard calibration curve is prepared using dilutions of purified fibrinogen and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Human Factor V total antigen assay ELISA kit

(HFVKT-TOT)

Factor V (aka proaccelerin or labile factor) is a 2224 amino acid single chain glycoprotein. Factor V is activated to Factor Va by thrombin. Factor Va binds to Factor Xa and acts as a cofactor in accelerating the activation of prothrombin to thrombin. A genetic Factor V R506Q mutation has been shown to result in a resistance to activated protein C leading to venous thrombosis. The sensitive quantitative measurement of total human Factor V antigen in plasma samples is easily performed with this 96 well strip format ELISA kit. Factor V and Va will be detected by the assay. The concentration of Factor V in normal human plasma ranges from 6.6-8.25 ug/ml. The assay measures total human Factor V in the 0.938-60 ng/ml range. Samples giving human Factor V levels above 60 ng/ml should be diluted in blocking buffer before use. A 1:800 dilution for plasma is suggested for best results. Human Factor V will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-human Factor V primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of Factor V in the samples. A standard calibration curve is prepared using dilutions of purified Factor V and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Immobilized affinity purified sheep anti rat & mouse prorenin/renin

(SASRREN-GF-HT-I)

Affinity purified sheep polyclonal antibody to rat prorenin immobilized on agarose resin. This immobilized antibody is extremely useful for the affinity purification of mouse and rat prorenin/renin from kidney extracts or from cell culture media.

Immobilized affinity purified sheep anti human prorenin/renin

(SASHREN-GF-HT-I)

Affinity purified sheep polyclonal antibody to human renin immobilized on agarose resin. This immobilized antibody is extremely useful for the affinity purification of human prorenin/renin directly from plasma or from cell culture media.

Immobilized monoclonal antibody to human prekallikrein and kallikrein

(PK10G3-I)

Immobilized monoclonal antibody to human prekallikrein and kallikrein. This immobilized antibody is extremely useful for the affinity purification of prekallikrein and kallikrein from chromatography fractions or directly from human plasma.

Mouse Albumin ELISA Kit

(MSAKT)

Albumin is a water-soluble protein with considerable structural stability which makes up 60 percent of the total protein of plasma. It functions as a carrier of hormones, enzymes, fatty acids, metal ions, and medicinal products. The sensitive quantitative measurement of total mouse albumin (murine serum albumin, MSA) in plasma, serum, urine or other biological fluid samples is easily performed with this 96 well strip format ELISA kit. This kit has been validated for measurement of albumin in BALB/c mouse urine (33 ug/ml), nude mouse urine (19 ug/ml), and CD1 mouse plasma (29 mg/ml). Albumin is present in normal mouse plasma at a concentration of 20-30 mg/ml. The assay measures mouse albumin in the 1-1000 ng/ml range. Samples giving mouse albumin levels above 1000ng/ml should be diluted in 1X diluent before use. A 1:500,000 to 1:1,000,000 dilution for normal plasma or a 1:1,000 dilution for urine is suggested for best results. Mouse albumin will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, peroxidase labeled anti-mouse albumin primary antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of mouse albumin in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified mouse albumin and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Also available: High quality mouse albumin purified from US origin plasma

Anti Human cTnT, Clone 8C11

(CTNT8C11)

Mouse monoclonal antibody to human Cardiac Troponin T (cTnT). IgG fraction purified by immobilized Protein G. Detects cTnT in western blot under non-reducing and reducing conditions. Cross reacts in western blot with human Skeletal Troponin T (sTNT, TNNT1). Does not react with Skeletal Troponin T in ELISA. Clone 8C11-F7, isotype IgG1 Kappa.

50% off your first order! Please reference promotion code MP2103. Offer expires April 30, 2013.

Mouse Prolactin Receptor, chiMAX Fc Fusion Protein

(MPRLR-FC)

Prolactin is a neuroendocrine peptide hormone that is secreted primarily by the pituitary gland. Prolactin receptor is a class 1 cytokine transmembrane receptor that varies in size with tissue source and species. It consists of an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region. The extracellular domain (Ser21-Asp229) is expressed recombinantly, purified by chromatography, sterile filtered and lyophilized. >95percent pure by SDS-PAGE and biologically active as measured by inhibitory activity toward prolactin-induced proliferation of Nb2-11 rat lymphoma cells (Gout et al. (1980) Cancer Res. 40:2433). ED50 for this effect is typically < 10 ng/ml in the presence of 0.5 ng/ml of recombinant human Prolactin. Add deionized water to original volume, aliquot and freeze unused portion.

This chiMAX Fc Fusion Protein is glycosylated and expressed as a chimera with the Fc region of mouse immunoglobulin (heavy chain hinge, CH2 and CH3). Fc fusion proteins are typically more stable and resistant to degradation and clearance than native cytokines. Fc fusion proteins appear as a dimer in SDS-PAGE under non-reducing conditions.

50% off your first order! Please reference promotion code MP2103. Offer expires April 30, 2013.

Rat Prolactin Receptor, chiMAX Fc Fusion Protein

(RPRLR-FC)

Prolactin is a neuroendocrine peptide hormone that is secreted primarily by the pituitary gland. Prolactin receptor is a class 1 cytokine transmembrane receptor that varies in size with tissue source and species. It consists of an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region. The extracellular domain (Ser21-Asp229) is expressed recombinantly, purified by chromatography, sterile filtered and lyophilized. >95percent pure by SDS-PAGE and biologically active as measured by inhibitory activity toward prolactin-induced proliferation of Nb2-11 rat lymphoma cells (Gout et al. (1980) Cancer Res. 40:2433). ED50 for this effect is typically < 50 ng/ml in the presence of 0.5 ng/ml of recombinant human Prolactin. Add deionized water to original volume, aliquot and freeze unused portion.

This chiMAX Fc Fusion Protein is glycosylated and expressed as a chimera with the Fc region of mouse immunoglobulin (heavy chain hinge, CH2 and CH3). Fc fusion proteins are typically more stable and resistant to degradation and clearance than native cytokines. Fc fusion proteins appear as a dimer in SDS-PAGE under non-reducing conditions.

50% off your first order! Please reference promotion code MP2103. Offer expires April 30, 2013.

Human Prothrombin total antigen assay ELISA kit

(HPTKT-TOT)

Prothrombin is a single-chain vitamin K dependent 579 amino acid glycoprotein zymogen. Prothrombin levels are decreased by anticoagulant therapy, vitamin K deficiency and severe liver disease. Elevated plasma prothrombin is associated with a single nucleotide change at position 20210. The sensitive quantitative measurement of total human prothrombin antigen in plasma samples is easily performed with this 96 well strip format ELISA kit. Thrombin and thrombin-antithrombin complex will be detected by the assay. Prothrombin in normal human plasma ranges from 110-212 ug/ml with an average concentration of 150 ug/ml. The assay measures total human prothrombin in the 0.25-100 ng/ml range. Samples giving human prothrombin levels above 100 ng/ml should be diluted in blocking buffer before use. A 1:5,000 to 1:40,000 dilution for plasma is suggested for best results. Human prothrombin will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, anti-human prothrombin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of prothrombin in the samples. A standard calibration curve is prepared using dilutions of purified prothrombin and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Mouse Thrombopoietin, chiMAX Fc Fusion Protein

(MTPO-FC)

Thrombopoietin (TPO), also known as Thrombopoiesis Stimulating Factor (TSF), is a glycoprotein hormone and the major stimulator of megakaryopoiesis and platelet production. TPO is expressed in liver, kidney, spleen, lung, bone marrow, and brain. The TPO receptor is a product of the proto-oncogene c-mpl and displays homology with type I cytokine receptor superfamily members. The receptor is present mainly on hematopoietic stem cells, megakaryocytic progenitors, megakaryocytes, and platelets. TPO is the primary regulator of platelet production by megakaryocytes. It stimulates the proliferation of hematopoietic stem cells, primitive progenitors, megakaryocytes, and platelets. Analogous to the effect of erythropoietin (EPO), the primary mode of action of TPO is inhibition of apoptosis of its target cells. By contrast, TPO is strongly proapoptotic in the brain and acts as a counterpart of EPO which has neuroprotective properties.
Mouse TPO (Ser22-Thr356) is expressed recombinantly, purified by chromatography, sterile filtered and lyophilized. Mouse TPO can be used for a variety of applications, including megakaryocyte proliferation and differentiation in vitro, in vitro expansion of hematopoietic stem cells, in vitro platelet activation and apoptosis assays. >95percent pure by SDS-PAGE and biologically active as measured in a proliferation assay with human TF-1 erythroleukemia cells (Drexler et al. (1997) Leukemia 11:541-551). The ED50 for this activity is typically < 5 ng/ml. Add deionized water to original volume, aliquot and freeze unused portion.

This chiMAX Fc Fusion Protein is glycosylated and expressed as a chimera with the Fc region of mouse immunoglobulin (heavy chain hinge, CH2 and CH3). Fc fusion proteins are typically more stable and resistant to degradation and clearance than native cytokines. This protein appears in multiple glycoforms in SDS-PAGE under both non-reducing and reducing conditions.

50% off your first order! Please reference promotion code MP2103. Offer expires April 30, 2013.

Mouse Erythropoietin, chiMAX Fc Fusion Protein

(MEPO-FC)

Erythropoietin (EPO) is a glycoprotein hormone related to Thrombopoietin which stimulates erythrocyte formation by inhibiting apoptosis of early erythroid precursors. Mouse EPO (Ala27-Arg192) is expressed recombinantly, purified by chromatography, sterile filtered and lyophilized. >95percent pure by SDS-PAGE and biologically active as measured in a proliferation assay with human TF-1 erythroleukemia cells (Kitamura et al. (1989) J. Cell Physiol. 140:323). The ED50 for this activity is typically < 5 ng/ml. Add deionized water to original volume, aliquot and freeze unused portion.

This chiMAX Fc Fusion Protein is glycosylated and expressed as a chimera with the Fc region of mouse immunoglobulin (heavy chain hinge, CH2 and CH3). Fc fusion proteins are typically more stable and resistant to degradation and clearance than native cytokines. Fc fusion proteins appear as a dimer in SDS-PAGE under non-reducing conditions.

50% off your first order! Please reference promotion code MP2103. Offer expires April 30, 2013.

Human Complement C3 ELISA kit

(HC3KT)

Complement Component 3 (C3), the most abundant serum complement component, is a disulfide-linked 185kDa 1,637 amino acid glycoprotein which supports the classical, alternative, and lectin pathways of complement activation. C3 is proteolytically activated by C3-convertase to the anaphylatoxin C3a and the opsonizing agent C3b. Serum concentrations of C3 are increased during acute and chronic inflammation such as rheumatoid arthritis, and are decreased due to increased consumption or autoimmune disorders such as systemic lupus erythematosus. The sensitive quantitative measurement of total human C3 antigen in plasma, serum, urine, milk, saliva and cell culture samples is easily performed with this 96 well strip format ELISA kit. C3 in normal human plasma ranges from 0.9-1.9 mg/ml with an average concentration of 1.39 mg/ml. The assay measures total human C3 in the 0.02-10 ng/ml range. Samples giving human C3 levels above 10 ng/ml should be diluted in blocking buffer before use. For best results, dilute plasma and serum samples 1:100,000 to 1:1,000,000, saliva samples to 1:100, urine samples 1:2 to 1:10, and milk samples 1:1,000 to 1:10,000. Human C3 will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-human C3 primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with streptavidin conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of C3 in the samples. A standard calibration curve is prepared using dilutions of purified C3and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Sheep anti rat & mouse prorenin/renin IgG fraction, High Titer

(SASRREN-GF-HT)

Polyclonal antibody (host sheep) to rat & mouse prorenin. Affinity purified IgG fraction.

FITC labeled mouse monoclonal to human uPA, clone 3A108

(HUPA3A108-FITC)

FITC labeled monoclonal antibody directed to the N-terminal alpha (light) chain region of human uPA, which includes the Amino-terminal Fragment (ATF) region. IgG fraction purified by immobilized Protein G. Isotype IgG.

FITC labeled mouse monoclonal to human uPA, clone 3G65

(HUPA3G65-FITC)

FITC labeled monoclonal antibody directed to the C-terminal beta (heavy) chain region of human uPA, which includes the active site. IgG fraction purified by immobilized Protein G. Isotype IgG.

Human PAI-1 (stable mutant – N-terminal poly-histidine tag)

(6X-HISCPAI)

A patent pending mutant of human PAI-1 containing four mutations that stabilize the activity of PAI-1 has been made available. The N-terminus has been additionally modified with the introduction of a 6-X histidine tag. The tag allows for the immobilization of functionally active PAI-1 onto surfaces such as metal chelate microtiter plates or Ni+2 resins. Many new applications for PAI-1 can be envisioned with this unique reagent.

Armenian Hamster IgG, Protein G Purified

(AHT-GF)

Purified from normal serum by immobilized Protein G. >95 percent pure by SDS-PAGE and preservative free.

Anhydrotrypsin

(ANHT)

Treatment of trypsin with phenylmethylsulfonyl fluoride (PMSF) followed by a strong base leads to the conversion of Serine 195 to dehydroalanine at the active site (1). Alpha and beta forms of anhydrotrypsin are separated by immobilized soybean trypsin inhibitor. The activity of anhydrotrypsin is greatly reduced compared to native trypsin as quantified by chromogenic substrate (2). Anhydrotrypsin provided by our company is the single chain beta-form prepared from Type XIII bovine trypsin.
References
1. Ako, H. et al. (1972) Biochem Biophys Res Commun. 47:1402-1407.
2. Olson, S.T. et al. (1995) J. Biol. Chem. 270:30007-30017.

Immobilized Anhydrotrypsin

(ANHT-I)

Immobilized anhydrotrypsin selectively adsorbs peptides with Arg, Lys or AECys (S-aminoethyl cysteine) residues at the C-terminus under weak acidic conditions (1). Strongly acidic conditions are used to elute the bound peptides. Peptides with these amino acids at the N-terminus or internally, as well as free amino acids are not bound. Serine protease inhibitors such as ecotin and PAI-1 may also be purified on this resin (2). May be used repeatedly.
References
1. Kumazaki, T. et al. (1987) J. Biochem. (Tokyo) 102:1539-1546.
2. Blouse, G. E. et al. (2003) Biochemistry 42:12260-12272.

Human PAI -1 Elastase specific mutant

(APAI)

A single mutation at the P1 position of PAI-1 alters the target specificity of PAI-1 from the plasminogen activators tPA and uPA to elastase. PAI-1 is normally a substrate for pancreatic and neutrophil elastase becoming cleaved at the P3 position. This mutant contains a P1 Alanine in place of the wild type Arginine residue resulting in an inhibitor of elastase as potent as alpha-1PI (antitrypsin).

Rabbit anti BSA antiserum

(ASBSA)

Polyclonal antiserum (host rabbit).

Rabbit anti BSA IgG fraction

(ASBSA-GF)

Polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti BSA IgG fraction, biotin labeled

(ASBSA-GF-BIO)

Biotin labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti BSA IgG fraction, fluorescein labeled

(ASBSA-GF-FITC)

FITC labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti BSA IgG fraction, HRP labeled

(ASBSA-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti BSA, affinity purified

(ASBSA-GF-HT)

Polyclonal antibody (host rabbit). Affinity purified by immobilized BSA.

Anti Chicken Plasminogen IgG

(ASCKPG-GF)

Polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti human antiplasmin antiserum

(ASHA2AP)

Polyclonal antiserum (host rabbit).

Rabbit anti human antiplasmin IgG fraction

(ASHA2AP-GF)

Polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti human antiplasmin IgG fraction, biotin labeled

(ASHA2AP-GF-BIO)

Biotin labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti human antiplasmin IgG fraction, FITC labeled

(ASHA2AP-GF-FITC)

FITC labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti human antiplasmin IgG fraction, HRP labeled

(ASHA2AP-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti human amino terminal fragment of uPA, affinity purified

(ASHATF-GF-HT)

Polyclonal antibody (host rabbit). Affinity purified by immobilized human ATF.

Anti-Cathepsin B, Human Liver, Rabbit Standard Antisera

(ASHCB-AS)

Polyclonal antibody (host rabbit). Gamma globulin purified by ammonium sulfate fractionation. Reacts to human liver cathepsin B and does not react with human liver cathepsin D, H, and L.

Anti-Cathepsin D, Human Liver, Rabbit Standard Antisera

(ASHCD-AS)

Polyclonal antibody (host rabbit). Gamma globulin purified by ammonium sulfate fractionation. Reacts to human liver cathepsin D and does not react with human liver cathepsin B, H, and L.

Anti-Cathepsin G, Human Neutrophil, Rabbit Standard Antisera

(ASHCG-AS)

Polyclonal antibody (host rabbit). Gamma globulin purified by ammonium sulfate fractionation. Reacts to human neutrophil cathepsin G. Immunoelectrophoresis: Single arc vs purified human neutrophil cathepsin G.

Anti-Cathepsin H, Human Liver, Rabbit Standard Antisera

(ASHCH-AS)

Polyclonal antibody (host rabbit). Gamma globulin purified by ammonium sulfate fractionation. Reacts to human liver cathepsin H and does not react with human liver cathepsin B, D and L.

Anti-Catalase, Human Erythrocyte, Rabbit IgG Fraction

(ASHEC-GF)

Polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A. Reacts to human erythrocyte catalase.

Rabbit anti human fibrinogen antiserum

(ASHFBGN)

Polyclonal antiserum (host rabbit).

Rabbit anti human fibrinogen IgG fraction

(ASHFBGN-GF)

Polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti human fibrinogen IgG fraction, biotin labeled

(ASHFBGN-GF-BIO)

Biotin labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti human fibrinogen IgG fraction, FITC labeled

(ASHFBGN-GF-FITC)

FITC labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti human fibrinogen IgG fraction, HRP labeled

(ASHFBGN-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti human fibrinogen, affinity purified

(ASHFBGN-GF-HT)

Polyclonal antibody (host rabbit). Affinity purified by immobilized human fibrinogen.

Rabbit anti human Factor VII antiserum

(ASHFVII)

Polyclonal antiserum (host rabbit) to human Factor VII.

Rabbit anti human Factor VII IgG fraction

(ASHFVII-GF)

Polyclonal antibody (host rabbit) to human Factor VII. IgG fraction purified by immobilized Protein A.

Rabbit anti human Factor VII IgG fraction, biotin labeled

(ASHFVII-GF-BIO)

Biotin labeled polyclonal antibody (host rabbit) to human Factor VII. IgG fraction purified by immobilized Protein A.

Rabbit anti human Factor VII IgG fraction, FITC labeled

(ASHFVII-GF-FITC)

FITC labeled polyclonal antibody (host rabbit) to human Factor VII. IgG fraction purified by immobilized Protein A.

Rabbit anti human Factor VII IgG fraction, HRP labeled

(ASHFVII-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host rabbit) to human Factor VII. IgG fraction purified by immobilized Protein A.

Rabbit anti human Factor VII IgG fraction, affinity purified

(ASHFVII-GF-HT)

Polyclonal antibody (host rabbit). Affinity purified by immobilized human factor VII.

Rabbit anti human factor X antiserum

(ASHFX)

Polyclonal antiserum (host rabbit) to human factor X.

Rabbit anti human factor X IgG fraction

(ASHFX-GF)

Polyclonal antibody (host rabbit) to human factor X. IgG fraction purified by immobilized Protein A.

Rabbit anti human factor X IgG fraction, biotin labeled

(ASHFX-GF-BIO)

Biotin labeled polyclonal antibody (host rabbit) to human factor X. IgG fraction purified by immobilized Protein A.

Rabbit anti human factor X IgG fraction, FITC labeled

(ASHFX-GF-FITC)

FITC labeled polyclonal antibody (host rabbit) to human factor X. IgG fraction purified by immobilized Protein A.

Rabbit anti human factor X IgG fraction, HRP labeled

(ASHFX-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host rabbit) to human factor X. IgG fraction purified by immobilized Protein A.

Anti-Myeloperoxidase, Human Neutrophil, Rabbit IgG Fraction

(ASHMPO-GF)

Polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A. Reacts to human neutrophil myeloperoxidase.

Anti-Elastase, Human Neutrophil, Rabbit Standard Antisera

(ASHNE-AS)

Polyclonal antibody (host rabbit). Gamma globulin purified by ammonium sulfate fractionation. Reacts to human neutrophil elastase, and does not react to human neutrophil cathepsin G, myeloperoxidase, or proteinase 3.

Anti-Amylase, Human Pancreas, Rabbit Standard Antisera

(ASHPA-AS)

Polyclonal antibody (host rabbit). Gamma globulin purified by ammonium sulfate fractionation. Reacts to human pancreatic amylase.

Rabbit anti human PAI-1 antiserum

(ASHPAI)

Polyclonal antiserum (host rabbit).

Rabbit anti human PAI-1 IgG fraction

(ASHPAI-GF)

Polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti human PAI-1 IgG fraction, biotin labeled

(ASHPAI-GF-BIO)

Biotin labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti human PAI-1 IgG fraction, fluorescein labeled

(ASHPAI-GF-FITC)

FITC labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti human PAI-1 IgG fraction, HRP labeled

(ASHPAI-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti human PAI-1, affinity purified

(ASHPAI-GF-HT)

Polyclonal antibody (host rabbit). Affinity purified by immobilized human PAI-1.

Anti-Kallikrein, Human Plasma, Rabbit IgG Fraction

(ASHPK-GF)

Polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A. Reacts to human plasma kallikrein.

Rabbit anti human prorenin antiserum

(ASHPREN)

Polyclonal antiserum (host rabbit) to human prorenin.

Rabbit anti human prorenin IgG fraction

(ASHPREN-GF)

Polyclonal antibody (host rabbit) to human prorenin. IgG fraction purified by immobilized Protein A.

Rabbit anti human prorenin IgG fraction, biotin labeled

(ASHPREN-GF-BIO)

Biotin labeled polyclonal antibody (host rabbit) to human renin. IgG fraction purified by immobilized Protein A.

Rabbit anti human prorenin IgG fraction, FITC labeled

(ASHPREN-GF-FITC)

FITC labeled polyclonal antibody (host rabbit) to human prorenin. IgG fraction purified by immobilized Protein A.

Rabbit anti human prorenin IgG fraction, HRP labeled

(ASHPREN-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host rabbit) to human prorenin. IgG fraction purified by immobilized Protein A.

Rabbit anti human tPA antiserum

(ASHTPA)

Polyclonal antiserum (host rabbit).

Rabbit anti human tPA IgG fraction

(ASHTPA-GF)

Polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti human tPA IgG fraction, biotin labeled

(ASHTPA-GF-BIO)

Biotin labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti human tPA IgG fraction, fluorescein labeled

(ASHTPA-GF-FITC)

FITC labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti human tPA IgG fraction, HRP labeled

(ASHTPA-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti human tPA, affinity purified

(ASHTPA-GF-HT)

Polyclonal antibody (host rabbit). Affinity purified by immobilized human tPA.

Anti-Trypsin, Human Pancreas, Rabbit IgG Fraction

(ASHTRYP-GF)

Polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A. Reacts to human pancreatic trypsin.

Rabbit anti human uPA antiserum

(ASHUPA)

Polyclonal antiserum (host rabbit).

Rabbit anti human uPA IgG fraction

(ASHUPA-GF)

Polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti human uPA IgG fraction, biotin labeled

(ASHUPA-GF-BIO)

Biotin labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti human uPA IgG fraction, fluorescein labeled

(ASHUPA-GF-FITC)

FITC labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti human uPA IgG fraction, HRP labeled

(ASHUPA-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti human uPA, affinity purified

(ASHUPA-GF-HT)

Polyclonal antibody (host rabbit). Affinity purified by immobilized human uPA.

Rabbit anti human vitronectin antiserum

(ASHVN)

Polyclonal antiserum (host rabbit).

Rabbit anti human vitronectin IgG fraction

(ASHVN-GF)

Polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti human vitronectin IgG fraction, biotin labeled

(ASHVN-GF-BIO)

Biotin labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti human vitronectin IgG fraction, FITC labeled

(ASHVN-GF-FITC)

FITC labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti human vitronectin IgG fraction, HRP labeled

(ASHVN-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti human vitronectin IgG fraction, affinity purified

(ASHVN-GF-HT)

Polyclonal antibody (host rabbit). Affinity purified by immobilized human vitronectin.

Rabbit anti mouse antiplasmin antiserum

(ASMA2AP)

Polyclonal antiserum (host rabbit).

Rabbit anti mouse antiplasmin IgG fraction

(ASMA2AP-GF)

Polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti mouse antiplasmin IgG fraction, biotin labeled

(ASMA2AP-GF-BIO)

Biotin labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti mouse antiplasmin IgG fraction, FITC labeled

(ASMA2AP-GF-FITC)

FITC labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti mouse antiplasmin IgG fraction, HRP labeled

(ASMA2AP-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti mouse fibrinogen antiserum

(ASMFBGN)

Polyclonal antiserum (host rabbit). Cross reacts with rat fibrinogen.

Rabbit anti mouse fibrinogen IgG fraction

(ASMFBGN-GF)

Polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A. Cross reacts with rat fibrinogen.

Rabbit anti mouse fibrinogen IgG fraction, biotin labeled

(ASMFBGN-GF-BIO)

Biotin labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A. Cross reacts with rat fibrinogen.

Rabbit anti mouse fibrinogen IgG fraction, FITC labeled

(ASMFBGN-GF-FITC)

FITC labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A. Cross reacts with rat fibrinogen.

Rabbit anti mouse fibrinogen IgG fraction, HRP labeled

(ASMFBGN-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A. Cross reacts with rat fibrinogen.

Rabbit anti mouse fibrinogen, affinity purified

(ASMFBGN-GF-HT)

Polyclonal antibody (host rabbit). Affinity purified by immobilized mouse fibrinogen. Cross reacts with rat fibrinogen.

Rabbit anti mouse PAI-1 antiserum

(ASMPAI)

Polyclonal antiserum (host rabbit).

Rabbit anti mouse PAI-1 IgG fraction

(ASMPAI-GF)

Polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti mouse PAI-1 IgG fraction, biotin labeled

(ASMPAI-GF-BIO)

Biotin labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti mouse PAI-1 IgG fraction, fluorescein labeled

(ASMPAI-GF-FITC)

FITC labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti mouse PAI-1 IgG fraction, HRP labeled

(ASMPAI-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti mouse PAI-1, affinity purified

(ASMPAI-GF-HT)

Polyclonal antibody (host rabbit). Affinity purified by immobilized mouse PAI-1.

Rabbit anti mouse plasminogen antiserum

(ASMPLG)

Polyclonal antiserum (host rabbit).

Rabbit anti mouse plasminogen IgG fraction

(ASMPLG-GF)

Polyclonal antibody (host rabbit).

Rabbit anti mouse plasminogen IgG fraction, biotin labeled

(ASMPLG-GF-BIO)

Biotin labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti mouse plasminogen IgG fraction, fluorescein labeled

(ASMPLG-GF-FITC)

FITC labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti mouse plasminogen IgG fraction, HRP labeled

(ASMPLG-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti mouse plasminogen, affinity purified

(ASMPLG-GF-HT)

Polyclonal antibody (host rabbit). Affinity purified by immobilized mouse plasminogen.

Rabbit anti mouse tPA antiserum

(ASMTPA)

Polyclonal antiserum (host rabbit).

Rabbit anti mouse tPA IgG fraction

(ASMTPA-GF)

Polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti mouse tPA IgG fraction, biotin labeled

(ASMTPA-GF-BIO)

Biotin labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti mouse tPA IgG fraction, fluorescein labeled

(ASMTPA-GF-FITC)

Fluorescein labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti mouse tPA IgG fraction, HRP labeled

(ASMTPA-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti mouse tPA, affinity purified

(ASMTPA-GF-HT)

Polyclonal antibody (host rabbit). Affinity purified by immobilized mouse tPA.

Rabbit anti mouse uPA antiserum

(ASMUPA)

Polyclonal antiserum (host rabbit).

Rabbit anti mouse uPA IgG fraction

(ASMUPA-GF)

Polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti mouse uPA IgG fraction, biotin labeled

(ASMUPA-GF-BIO)

Biotin labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti mouse uPA IgG fraction, fluorescein labeled

(ASMUPA-GF-FITC)

FITC labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti mouse uPA IgG fraction, HRP labeled

(ASMUPA-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti mouse uPA, affinity purified

(ASMUPA-GF-HT)

Polyclonal antibody (host rabbit). Affinity purified by immobilized mouse uPA.

Rabbit anti mouse vitronectin antiserum

(ASMVN)

Polyclonal antiserum (host rabbit).

Rabbit anti mouse vitronectin IgG fraction

(ASMVN-GF)

Polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti mouse Vitronectin IgG fraction, biotin labeled

(ASMVN-GF-BIO)

Biotin labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti mouse Vitronectin IgG fraction, FITC labeled

(ASMVN-GF-FITC)

FITC labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti mouse Vitronectin IgG fraction, HRP labeled

(ASMVN-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti mouse Vitronectin IgG fraction, affinity purified

(ASMVN-GF-HT)

Polyclonal antibody (host rabbit). Affinity purified by immobilized mouse vitronectin.

Rabbit anti porcine fibrinogen antiserum

(ASPFBGN)

Polyclonal antiserum (host rabbit).

Rabbit anti porcine fibrinogen IgG fraction

(ASPFBGN-GF)

Polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti porcine fibrinogen IgG fraction, biotin labeled

(ASPFBGN-GF-BIO)

Biotin labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti porcine fibrinogen IgG fraction, FITC labeled

(ASPFBGN-GF-FITC)

FITC labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti porcine fibrinogen IgG fraction, HRP labeled

(ASPFBGN-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti porcine fibrinogen, affinity purified

(ASPFBGN-GF-HT)

Polyclonal antibody (host rabbit). Affinity purified by immobilized porcine fibrinogen.

Rabbit anti rat PAI-1 antiserum

(ASRPAI)

Polyclonal antiserum (host rabbit).

Rabbit anti rat PAI-1 IgG fraction

(ASRPAI-GF)

Polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti rat PAI-1 IgG fraction, biotin labeled

(ASRPAI-GF-BIO)

Biotin labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti rat PAI-1 IgG fraction, fluorescein labeled

(ASRPAI-GF-FITC)

FITC labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti rat PAI-1 IgG fraction, HRP labeled

(ASRPAI-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host rabbit). IgG fraction purified by immobilized Protein A.

Rabbit anti rat PAI-1, affinity purified

(ASRPAI-GF-HT)

Polyclonal antibody (host rabbit). Affinity purified by immobilized rat PAI-1.

Rabbit anti rat plasmin, affinity purified

(ASRPLM-GF-HT)

Polyclonal antibody (host rabbit). Affinity purified by immobilized rat plasmin.

Affinity Purified Rabbit Anti Rat uPA

(ASRUPA-GF-HT)

Polyclonal antibody (host rabbit). Affinity purified by immobilized rat uPA.

Anti-Trypsin Inhibitor, Soybean, Rabbit IgG Fraction

(ASSBTI-GF)

Polyclonal antibody (host rabbit). Monospecific to Trypsin Inhibitor (Soy Bean) by IEP. Some cross reactivity with Trypsin Inhibitor of other species and tissues may occur. Immunoglobulin purified by delipidation, salt fractionation and ion-exchange chromatography.

Avidin, HRP Labeled

(AVI-HRP)

Horseradish peroxidase conjugated purified avidin from chicken egg white. Specific for biotin, biotinylated proteins, or biotinylated antibodies. Recommended starting dilution for ELISA is 1:1,000 but proper working dilution must be optimized by the end user.
Please be aware that our minimum order is $50.

Avidin Coated Plate

(AVI-PLATE)

96 well plate with 8 removable strips coated with Avidin at 100 ul/well and blocked with 300 ul/well. Ideal for specific binding of biotin-conjugated proteins and antibodies. Binding performance is characterized with biotinylated rabbit IgG followed by detection with HRP labeled secondary antibody.

Advantages of Avidin Plates from Molecular Innovations:
Senstitive – detect down to 0.1 ng/ml of biotinylated IgG
Specific – low background with high signal to noise ratio
High Capacity – binds >100 ng/ml of biotinylated IgG
Robust – broad dynamic range
Convenient – Ready to use plates are supplied pre-blocked to save time
Accurate – inter-assay CV is less than or equal to 3.47percent up to 100 ng/ml of biotinylated IgG

Bovine Fibrinogen

(BFBGN)

Prepared from fresh bovine plasma using several chromatographic steps. Plasminogen depleted by lysine affinity chromatography. Thaw at 37C without agitation until completely liquid, then gently mix before use. Keep fibrinogen at 25-37C, aliquot and flash freeze unused portion.

FITC Labeled Bovine Fibrinogen

(BFBGN-FITC)

Bovine fibrinogen amino terminal labeled with fluorescein isothiocyanate (FITC). Prepared from fresh bovine plasma using several chromatographic steps. Plasminogen depleted by lysine affinity chromatography. Thaw at 37C without agitation until completely liquid, then gently mix before use. Keep fibrinogen at 25-37C, aliquot and flash freeze unused portion.

Bovine plasminogen

(BPLG)

Prepared from fresh bovine plasma by immobilized lysine chromatography.

Bovine plasmin

(BPLM)

Prepared from plasminogen by activation with immobilized human uPA. 100 percent functionally active plasmin is purified from the activation reaction by immobilized SBTI. Plasmin will undergo rapid auto proteolysis in the absence of benzamidine and should be used quickly once thawed. Aliquot to avoid freeze-thaw cycles.
Partial cleavage of bovine plasmin between kringles 3 & 4 occurs upon activation, producing a low molecular weight band. This midiplasmin is 100 percent active (Christensen et al. Biochem J. 305 (Pt 1):97-102, 1995).

Anti BSA, Clone 12C8D4

(BSA12C8D4)

Monoclonal antibody (host mouse). IgG fraction purified by immobilized Protein A. Isotype IgG1Kappa.

Anti BSA, Clone 9E2C2

(BSA9E2C2)

Monoclonal antibody (host mouse). IgG fraction purified by immobilized Protein A. Isotype IgG1Kappa.

Bovine multimeric vitronectin

(BVN)

Prepared from fresh bovine plasma using urea as a denaturant.

Chicken plasminogen

(CKPG)

Prepared from fresh chicken plasma by immobilized lysine chromatography.

Plasminogen Depleted Chicken Serum

(CKPLA-SER-PG)

Prepared from frozen chicken serum.

Chicken plasmin

(CKPLM)

Prepared from plasminogen by activation with immobilized human uPA. 100 percent functionally active plasmin is purified from the activation reaction by immobilized SBTI. Plasmin will undergo rapid auto proteolysis in the absence of benzamidine and should be used quickly once thawed. Aliquot to avoid freeze-thaw cycles.

Human PAI-1 (stable mutant form)

(CPAI)

The human form of PAI-1 contains the following four mutations: K154T, Q319L, M354I and N150H. These mutations combine to confer great stability to the otherwise labile molecule essentially locking in into the active conformation. It is an ideal choice for in vivo studies.

Human PAI-1 stable mutant NBD labeled at the scissile bond of the reactive loop

(CPAI-P1NBD)

Mutagenesis of the P1′ methionine residue (Met347) at the P1-P1′ scissile bond of human PAI-1 stable mutant (K154T, Q319L, M354I and N150H) to cysteine followed by incorporation of NBD has produced a reporter PAI-1 with extended half life. The properties of this interesting new reagent have not been fully characterized.

Human PAI-1 stable mutant NBD labeled at the reactive center loop

(CPAI-P9NBD)

Mutagenesis of the P9 serine residue (Ser338) on the reactive center loop of human PAI-1 stable mutant (K154T, Q319L, M354I and N150H) to cysteine followed by incorporation of NBD has produced a reporter PAI-1 with extended half life. The properties of this interesting new reagent have not been fully characterized.

Human PAI-1 (stable vitronectin reduced binding mutant)

(CPAI-Q123K)

The Q123K point mutation on the human PAI-1 stable mutant background (K154T, Q319L, M354I and N150H) results in PAI-1 with a greatly extended half life and reduced binding to the important ligand vitronectin.
References
Jensen, J.K. et al. (2004) FEBS Lett. 556: 175-179.

Human PAI-1 tPA complex

(CPAI-TPA)

Human PAI-1 stable mutant is reacted with an excess of human sc-tPA at physiological pH. The resulting 1:1 molar complex is purified from the remaining tPA by cobalt metal affinity chromatography resin.

Human PAI-1 uPA complex

(CPAI-UPA)

Human PAI-1 stable mutant is reacted with an excess of human urokinase at physiological pH. Excess uPA is removed from the resulting 1:1 molar complex by immobilized benzamidine resin.

Corn Trypsin Inhibitor

(CTI)

To begin the purification process, CTI is extracted from the kernels of fresh sweet corn into a physiologic buffer. The extract is then de-fatted using acetone, and the protein is further purified by employing gel filtration and ion-exchange chromatography. The final CTI preparation appears as a single band by SDS-PAGE analyses under both reducing and non-reducing conditions. Preparations of CTI are tested for the ability to prolong the aPTT assay without affecting the PT assay. The specific activity of each lot of CTI is determined, and one unit is defined as the amount of CTI required to double the aPTT of normal human plasma.

Cyno Monkey IgG, Protein A Purified

(CY-GF)

Purified from normal serum by immobilized Protein A. >95 percent pure by SDS-PAGE and preservative free.

Dog Fibrinogen

(DFBGN)

Prepared from fresh US origin dog (canine) plasma using several chromatographic steps. Plasminogen depleted by lysine affinity chromatography. Thaw at 37C without agitation until completely liquid, then gently mix before use. Keep fibrinogen at 25-37C, aliquot and flash freeze unused portion.

FITC Labeled Dog Fibrinogen

(DFBGN-FITC)

Canine fibrinogen amino terminal labeled with fluorescein isothiocyanate (FITC). Prepared from fresh dog plasma using several chromatographic steps. Plasminogen depleted by lysine affinity chromatography. Thaw at 37C without agitation until completely liquid, then gently mix before use. Keep fibrinogen at 25-37C, aliquot and flash freeze unused portion.

Donkey IgG, Protein G Purified

(DK-GF)

Purified from normal serum by immobilized Protein G. >95 percent pure by SDS-PAGE and preservative free.

Dog Prorenin, C-terminal 8x His tag

(DPREN-HIS)

Recombinantly produced in HEK cell culture and purified by chelated metal affinity chromatography. Contains a 8X-Histidine tag at C terminus for purification.

Dog Renin, C-terminal 8x His tag

(DREN-HIS)

Renin is an aspartyl protease that is specific for only one substrate, angiotensinogen. Molecular Innovations dog renin is produced from the proenzyme prorenin by proteolytic cleavage of a 43 amino acid N-terminal prosegment using limited enzymatic digestion by immobilized trypsin. Conversion to active renin is >98 percent.

Active Dog Urokinase

(DUPA)

Active two-chain canine urokinase (uPA), recombinantly produced in insect cells.

Chromogenic substrate for plasmin

(D-VLK-pNA)

The plasmin substrate provide by our company is chemically and functionally identical to Chromogenix S-2251. Each 25 mg of substrate is lyophilized with 60 mg mannitol as a bulking agent. For best results add 4.5 ml of 1mM HCl directly to vial to make a 10mM stock solution then aliquot and freeze. Dilute 1:50 in PBS or TBS to make an 0.2mM working solution. Add 0.1 ml test sample to 0.9 ml substrate and monitor color development at 405nm.

Formula: H-D-Val-Leu-Lys-pNA . 2HCl
Mr: 551.6 Da

Ecotin (E. coli serine protease inhibitor)

(ECOTIN)

Serine protease inhibitor purified from E. coli. At least 98percent pure as determined by SDS-PAGE. References: 1. Seymour, J.L. et al. (1994) Biochemistry 33:3949-3958.

E. Carinatus Viper Venom Prothrombin Activator

(ECV-II)

Purified from Echis Carinatus Viper (Indian saw-scaled viper) Venom. Metal dependant endopeptidase that cleaves the Arg332-Ile323 bond in prothrombin to form meizothrombin. This protease is inhibited by EDTA, DTT and o-phenanthroline. ECV-II is a single chain glycoprotein and is highly pure and homogenous by SDS-PAGE.

Immobilized porcine pancreatic elastase

(ELAS-I)

Cleaves PAI-1 between the P3 and P4 residues to create a loop-inserted species that is non-inhibitory towards the target enzymes uPA and tPA.

Human Fibrinogen

(FIB)

Prepared from fresh human plasma using several chromatographic steps. Plasminogen depleted by lysine affinity chromatography. Thaw at 37C without agitation until completely liquid, then gently mix before use. Keep fibrinogen at 25-37C, aliquot and flash freeze unused portion.

FITC Labeled Human Fibrinogen

(FIB-FITC)

Human fibrinogen amino terminal labeled with fluorescein isothiocyanate (FITC). Prepared from fresh human plasma using several chromatographic steps. Plasminogen depleted by lysine affinity chromatography. Thaw at 37C without agitation until completely liquid, then gently mix before use.

Anti Human Factor IX, Clone 13F42F6

(FIX13F42F6)

Mouse monoclonal antibody to human Factor IX. IgG fraction purified by immobilized Protein G. Isotype IgG1Kappa.

Fluorescein-Phe-Pro-Arg-CMK

(FL-INFPR)

Fluorescein labeled ATA-Phe-Pro-Arg-CMK.

Human PAI -1 Cathepsin G specific mutant

(FPAI)

A single mutation at the P1 position of PAI-1 alters the target specificity of PAI-1 from the plasminogen activators tPA and uPA to Cathepsin G. This mutant contains a P1 Phenylalanine in place of the wild type Arginine residue (R346F) resulting in an inhibitor with the specificity of antichymotrypsin.

Human sc-tPA, ATA-FPR-CMK inactivated

(FPRCK-TPA)

Prepared from human tPA by incubation with an excess of ATA-Phe-Pro-Arg-CMK. After labeling at the active site, remaining free peptide is removed by dialysis.

Anti Human Factor VII, Clone 11G42D8

(FVII11G42D8)

Mouse monoclonal antibody to Human Factor VII. IgG fraction purified by immobilized Protein G. Isotype IgG2bKappa.

Anti Human Factor X, Clone 3C8B9

(FX3C8B9)

Mouse monoclonal antibody to human Factor X. IgG fraction purified by immobilized Protein G. Isotype IgG1Kappa.

Anti Human Factor X, Clone 4E3F8

(FX4E3F8)

Mouse monoclonal antibody to human Factor X. IgG fraction purified by immobilized Protein G. Isotype IgG1.

Anti Mouse IgG, HRP Labeled

(GAM-HRP)

Horseradish peroxidase conjugated goat anti-mouse IgG, Heavy & Light chain specific. Affinity purified and adsorbed against bovine, horse, and human serum proteins. Monospecific for mouse IgG, heavy and light chains, as determined by immunoelectrophoresis against normal mouse serum. Cross-reactivity with normal bovine, horse, and human serum is Please be aware that our minimum order is $50.

Anti Rabbit IgG, HRP Labeled

(GAR-HRP)

Horseradish peroxidase conjugated goat anti-rabbit IgG, Heavy & Light chain specific. Affinity purified and adsorbed against bovine, horse, human, and mouse serum proteins. Monospecific for rabbit IgG, heavy and light chains, as determined by immunoelectrophoresis against normal rabbit serum. Cross-reactivity with normal bovine, horse, human, and mouse serum is Please be aware that our minimum order is $50.

Goat anti rabbit PAI-1 antiserum

(GASRbPAI)

Polyclonal antiserum (host goat).

Goat anti rabbit PAI-1 IgG fraction

(GASRbPAI-GF)

Polyclonal antibody (host goat). IgG fraction purified by immobilized Protein G.

Goat anti rabbit PAI-1 IgG fraction, biotin labeled

(GASRbPAI-GF-BIO)

Biotin labeled polyclonal antibody (host goat). IgG fraction purified by immobilized Protein G.

Goat anti rabbit PAI-1 IgG fraction, fluorescein labeled

(GASRbPAI-GF-FITC)

FITC labeled polyclonal antibody (host goat). IgG fraction purified by immobilized Protein G.

Goat anti rabbit PAI-1 IgG fraction, HRP labeled

(GASRbPAI-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host goat). IgG fraction purified by immobilized Protein G.

Goat anti rabbit PAI-1, affinity purified

(GASRbPAI-GF-HT)

Polyclonal antibody (host goat). Affinity purified by immobilized rabbit PAI-1.

Glu-Gly-Arg-Chloromethylketone

(GGACK)

Glu-Gly-Arg-Chloromethylketone. Also known as EGR-chloromethylketone, EGR-CMK, and GGACK.

Biotin Labeled Glu-Gly-Arg-Chloromethylketone

(GGACK-BIO)

Biotinylated Glu-Gly-Arg-Chloromethylketone (EGRCK). Potent and specific irreversible inhibitor of Factor Xa. Also known as EGR-chloromethylketone, EGR-CMK, EGRCK, and GGACK.

Human PAI-1 (Glycosylated – stable mutant)

(GLYCPAI)

Glycolyslated human PAI-1 produced in insect cells with a molecular weight of 46,000 Da. The stable mutant contains four mutations (K154T, Q319L, M354I and N150H) to confer additional stability to the otherwise labile molecule.

Glycosylated Human PAI-1 (active form)

(GLYHPAI-A)

Glycosylated wild type human PAI-1 produced in insect cells with molecular weight of ~46,000 Da. Active form.

Glycosylated Human PAI-1 (latent form)

(GLYHPAI-L)

Glycosylated wild type human PAI-1 produced in insect cells with molecular weight of ~46,000 Da. Latent form.

Human GPIIbIIIa

(GP2B3A)

Platelet membrane glycoproteins IIb and IIIa (GPIIb and GPIIIa) constitute the fibrinogen receptor and are required for platelet aggregation. Glycoprotein IIb consists of 2 disulfide-linked subunits GPIIb (MW = 125,000) and GPII (MW= 23,000) while GPIIIa has only one polypeptide chain (MW= 108,000). GPIIbIIIa migrates on gels as follows: GPIIb 136,000 non-reduced and 125,000 reduced. GPIIIa is 97,000 non-reduced and 108,000 reduced. Protein concentration is determined via the Bradford method.

Goat IgG, Protein G Purified

(GT-GF)

Purified from normal serum by immobilized Protein G. >95 percent pure by SDS-PAGE and preservative free.

Mouse monoclonal to mouse PAI-1

(H14H7)

Capture monoclonal antibody produced in mouse. IgG fraction purified by immobilized Protein A. Isotype IgG.

Mouse monoclonal to mouse PAI-1, biotin labeled

(H14H7-BIO)

Biotin labeled monoclonal antibody produced in mouse. IgG fraction purified by immobilized Protein A. Isotype IgG.

Mouse monoclonal to mouse tPA

(H27B6)

Capture monoclonal antibody produced in mouse. IgG fraction purified by immobilized Protein A. Isotype IgG.

Mouse monoclonal to mouse PAI-1

(H34G6)

Capture monoclonal antibody produced in mouse. IgG fraction purified by immobilized Protein A. Isotype IgG.

Inhibitory mouse monoclonal to mouse PAI-1

(H4B3)

Inhibitory monoclonal antibody produced in mouse. IgG fraction purified by immobilized Protein A. Isotype IgG.

Mouse monoclonal to mouse tPA

(H6C5)

Detection monoclonal antibody produced in mouse. IgG fraction purified by immobilized Protein A. Isotype IgG.

Mouse monoclonal to mouse uPA

(H77A10)

Capture monoclonal antibody produced in mouse. IgG fraction purified by immobilized Protein A. Isotype IgG.

Mouse monoclonal to mouse uPA

(H77B6)

Detection monoclonal antibody produced in mouse. IgG fraction purified by immobilized Protein A. Isotype IgG.

Alpha-1 Antichymotrypsin, Human Plasma

(HA1AC)

An acute-phase plasma protein found at 45 mg per 100 ml. ACT functions as a specific inhibitor of chymotrypsin-like serine proteases. Clinically, it is elevated in inflammatory conditions, some malignancies, Crohn’s disease, ulcerative colitis, and burn injuries. The PSA-ACT complex level is considered a biomarker for prostate cancer. Prepared from plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE. Add deionized water to original volume, aliquot and freeze unused portion.

Alpha-1-Antitrypsin, Human Plasma

(HA1AT)

An acute-phase plasma protein found at 290 mg per 100 ml that functions as a protease inhibitor. Clinically, its deficiency is associated with two major diseases: pulmonary emphysema and early onset/juvenile hepatic cirrhosis. It is elevated in inflammatory conditions, malignancies, liver disease and pregnancy and also after surgical trauma and use of oral contraceptives. The simultaneous quantitative determination of alpha-1-PI and ceruloplasmin permits differential diagnosis of liver afflictions. Prepared from plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE. Add deionized water to original volume, aliquot and freeze unused portion.

Alpha-1-Acid Glycoprotein, Human Plasma

(HA1GP)

Found in human plasma in concentrations of 55-140 mg per 100 ml. Classical standard glycoprotein for studies on the structure of the oligosaccharide units. Carbohydrate content is 40percent. Its biological significance is unknown although it can bind progesterone 15 times as strongly as albumin. Sialic-acid-deficient alpha-1-AG has an affinity for vitamin B-12. Clinically, alpha 1 acid glycoprotein is an acute-phase reactant that together with haptoglobin is an indicator of acute inflammation. The alpha 1 acid glycoprotein:haptolobin ratio is useful in studies of bone marrow disorders, hemolytic processes and metastases. Prepared from plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE. Add deionized water to original volume, aliquot and freeze unused portion.

Human alpha 2 antiplasmin

(HA2AP)

Efficiently inhibits the plasminogen-activator-induced lysis of fibrin clots. Found in plasma at about 70 ug per ml. Alpha-antiplasmin-deficiency is a rare coagulation disorder which allows unrestrained fibrinolytic activity. Individuals with this condition may receive therapeutic A2AP prior to surgery to prevent postoperative hemorrhaging. Prepared from plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE. Add deionized water to original volume, aliquot and freeze unused portion.

Human antiplasmin total antigen assay ELISA kit

(HA2APKT-TOT)

Alpha-2-antiplasmin is the major circulating inhibitor of plasmin. It plays a role in the regulation of intravascular fibrinolysis. Decreased levels of alpha-2-antiplasmin may play an important role in the increased capacity of the fibrinolytic function and may be beneficial in the treatment of thrombotic diseases, acute pulmonary embolism, and hepatic repair. The sensitive quantitative measurement of total human alpha-2-antiplasmin antigen in samples is easily performed with this 96 well strip format ELISA kit. The normal human concentration of antiplasmin is 70 ug/ml in plasma and 47.6 ug/ml in serum. The assay measures total antiplasmin in the 0.1-100 ng/ml range. Samples giving human antiplasmin levels above 100 ng/ml should be diluted in blocking buffer before use. A 1:10,000-20,000 dilution for plasma is suggested for best results. Human antiplasmin will bind to the capture antibody coated onto a micro titer plate. Free and complexed antiplasmin will bind to the plate and will be detected by the assay. After appropriate washing steps, biotin labeled anti human antiplasmin primary antibody binds to the antiplasmin. Excess antibody is washed away, and bound antibody is reacted with peroxidase conjugated streptavidin. TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of antiplasmin in the samples. A standard calibration curve is prepared using dilutions of purified antiplasmin and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Alpha-2-HS-Glycoprotein, Human Plasma

(HA2GP)

Found in human serum at 60 mg per 100 ml. This protein is a negative acute-phase reactant. Clinically, concentrations are reduced in cancer patients. AHSG levels are positively correlated with gestational diabetes and negatively correlated with neonatal skeletal development. Studies show AHSG to be present at high concentrations in the peritoneal fluids of patients with endometriosis. Prepared from plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE. Add deionized water to original volume, aliquot and freeze unused portion.

Alpha-2-Macroglobulin, Human Plasma

(HA2MG)

A major serum protein found at concentrations of 240 mg per 100 ml in men and 290 mg per 100 ml in women. Multifunctional, it promotes growth of mammalian cells in culture, stimulates the regeneration of lymphocytes in irradiated mice, possesses a transport function for zinc and is a proteinase inhibitor that controls the clotting and fibrinolytic system. Clinically levels are increased in liver cirrhosis, nephrotic syndrome, diabetes, and severe burn cases. Prepared from plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE. Add deionized water to original volume, aliquot and freeze unused portion.

Anti Human Antiplasmin

(HAP1E94)

For ELISA and western blot of human antiplasmin. Detects purified antiplasmin and antiplasmin in plasma, under both native and reducing conditions. Isotype IgG.

Human Activated Protein C

(HAPC)

Activated Protein C (APC) is a serine protease derived from the two chain vitamin K dependent zymogen Protein C. APC inhibits blood coagulation through the selective inactivation of the cofactors Va and VIIIa. APC is prepared from Protein C by activation with purified thrombin. This thrombin is removed after activation by ion exchange chromatography. Activated Protein C purity is determined by SDS-PAGE and shows complete reduction upon incubation with 2-mercaptoethanol.

Apolipoprotein A1, Human Plasma, HDL

(HAPO-A1)

75percent of Apo A in HDL is Apo AI. Levels of Apo AI are inversely related to the risk of coronary heart disease. Apo AI is also thought to activate LCAT (lecithin cholesterol acyl tranferase). In normal plasma, Apo AI levels range from 90-130 mg per 100 ml. Prepared from plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE.

Apolipoprotein A2, Human Plasma, HDL

(HAPO-A2)

25percent of Apo A in HDL is Apo AII. Levels of Apo AII in normal plasma range from 30-50 mg per 100 ml. The physiological role of the protein is unknown. Alcohol consumption, however, increases Apo AII levels. Prepared from plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE.

Apolipoprotein B, Human Plasma, LDL

(HAPO-B)

Apolipoprotein B is the dominant protein constituent of LDL. The concentration of Apo B in normal plasma is 90 mg per 100 ml. Apo B is thought to stabilize lipid emulsions, serve as a cofactor and modulator of enzymatic reactions, manage export of lipids out of cells and direct lipids to target organs. Apo B levels are positively correlated with the risk of coronary disease. Apo B levels may be a more sensitive predictor of cardiovascular risk than LDL levels and do not involve fasting for accurate measurement. Two forms of Apo B exist: Apo B-100 and Apo B-48. The first is found in VLDL and LDL and is produced by the liver. The second is found in chylomicrons and originates in the intestine. Prepared from fresh, non-frozen plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE. Add deionized water to original volume, aliquot and freeze unused portion.

Apolipoprotein C1, Human Plasma, VLDL

(HAPO-C1)

Apolipoprotein CI is one of the protein components of VLDL lipoprotein. The normal plasma concentration of Apo CI is 4-7 mg per 100 ml. Elevated levels of Apo CI may offer some protection from obesity and insulin-dependent diabetes. Some evidence exists that the Apo CI H2 gene is positively associated with late-onset Alzheimer’s disease. Prepared from fresh, non-frozen plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE. Add deionized water to original volume, aliquot and freeze unused portion.

Apolipoprotein C2, Human Plasma, VLDL

(HAPO-C2)

Apo CII activates lipoprotein lipase which then hydrolyzes VLDL triglyceride to form IDL (intermediate density lipoproteins). The concentration of Apo CII in normal plasma is 3-8 mg per 100 ml. Prepared from fresh, non-frozen plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE. Add deionized water to original volume, aliquot and freeze unused portion.

Apolipoprotein C3, Human Plasma, VLDL

(HAPO-C3)

Apo CIII inhibits the activation of lipoprotein lipase by Apo CII. Reduced levels of Apo CIII result in higher fatty acid uptake from plasma triglycerides into adipose tissue. Thus, Apo CIII is a potential target for treatment of obesity and insulin resistance. The normal plasma concentration of Apo CIII is 8-15 mg per 100 ml. Prepared from fresh, non-frozen plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE.

Apolipoprotein E, Human Plasma, VLDL

(HAPO-E)

Apolipoprotein E serves as a ligand for low density receptors and participates in the transport and redistribution of cholesterol and other lipids. Other functions include immunoregulation and cell growth modulation and differentiation. Apo E is thought to be involved in tissue repair as increased amounts of the protein are found at sites of peripheral nerve injury and regeneration. A mutant form is associated with familial type III hyperlipoproteinemia. The concentration of Apo E in normal plasma is 5 mg per 100 ml. Prepared from fresh, non-frozen plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE.

Apolipoprotein H, Human Plasma

(HAPO-H)

Apolipoprotein H, also known as beta2Glycoprotein-1 (B2GP-1) is a plasma glycoprotein that circulates at a concentration of 200 ug/mL (4mM). B2GP-1 has been identified as a constituent of chylomicrons, very low density lipoproteins and high density lipoproteins in Human plasma. It has also been demonstrated to bind phospholipids, heparin and platelets where it can modulate the activity of adenylate (19percent). Although the precise function(s) are as yet unknown, B2GP-1 has been shown to interfere with blood coagulation by competitively binding to phospholipids exposed during cell activation or damage. Recent evidence also implicates B2GP-1 as a cofactor recognized by some anti-phospholipid antibodies present in autoimmune disorders such as systemic lupus erythematosus (SLE).

Apotransferrin, Human Plasma

(HAPOTF)

Transferrin less the iron molecule. Like transferrin, apotransferrin has a physiological role in the transportation and distribution of iron among the body organs. It is also an important transport factor used in defined culture media. Purified to have less than 0.02 mg iron per gram of transferrin. Each human transferrin molecule has the ability to carry two iron ions in the ferric form (Fe3+). Dissolves in water at 10 mg per ml. Prepared from plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. Total protein determination by the Lowry method. >95percent pure and shows only one major band corresponding to the molecular weight of Transferrin by SDS-PAGE. Add deionized water to original volume, aliquot and freeze unused portion.

Horse anti-Human Factor V

(HASHFV-GF)

Polyclonal antibody (host horse). IgG fraction purified by immobilized Protein A.

Amino terminal fragment of human uPA

(HATF)

The amino terminal fragment (ATF) of uPA is purified from auto-catalytic digestion of recombinant human urokinase produced in insect cells. ATF contains the Kringle and Epidermal Growth Factor domains and is separated from LMW uPA by cleavage at the Lys135-Lys136 bond.

Human Antithrombin III

(HATIII)

Prepared from fresh human plasma using several chromatographic steps.

Immobilized human antithrombin

(HATIII-I)

May be used repeatedly. Protocol for purifying high affinity heparin with immobilized antithrombin:
1. Equilibrate immobilized antithrombin in TBS (0.1M Tris-HCl, 0.15M NaCl, pH 7.4) or PBS (0.05M Sodium Phosphate, 0.15M NaCl, pH 7.4).
2. Apply heparin in TBS or PBS. Binding capacity is ~0.1 mg high affinity heparin / ml resin and must be determined by the end user.
3. Wash and elute heparin with 3M NaCl in TBS or PBS.
4. Re-equilabrate resin in TBS or PBS.
5. Add 0.02percent Sodium Azide for storage.

Human Antithrombin III total antigen assay ELISA kit

(HATIIIKT-TOT)

Antithrombin III is a glycosylated plasma serine protease inhibitor that forms a stoichiometric complex with coagulation cascade enzymes. Antithrombin III inhibits alpha-Thrombin as well as Factor Xa, IXa, XIa and XIIa with heparin enhanced kinetics. Type 1 Antithrombin deficiency is characterized by decreased plasma antigen levels of Antithrombin III. The sensitive quantitative measurement of total human Antithrombin III antigen in plasma samples is easily performed with this 96 well strip format ELISA kit. An antithrombin concentration of 137 ug/ml in human reference plasma was determined by house testing at 1:100,000 and 1:200,000 dilutions. The assay measures total human Antithrombin IIII in the 0.01-10 ng/ml range. Samples giving human Antithrombin III levels above 10 ng/ml should be diluted in blocking buffer before use. A 1:100,000 to 1:400,000 dilution for plasma is suggested for best results. Human Antithrombin III will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, peroxidase labeled anti-human Antithrombin III primary antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of Antithrombin III in the samples. A standard calibration curve is prepared using dilutions of purified Antithrombin III and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

The Human Antithrombin III standard provided in this kit is calibrated against the WHO International Standard for Human Plasma Antithrombin (NIBSC Code 08/258).

Human beta-thrombin

(HBT)

Human beta-thrombin is prepared from purified a-thrombin by limited proteolysis with TPCK-treated trypsin. Beta-thrombin is generated by cleavage of the B-chain at the Arg106-Tyr107 bond. Purity is assessed by SDS-PAGE and activity is assessed using a fibrinogen clotting assay.

C-1 Esterase Inhibitor, Human Plasma

(HC1EIN)

A single chain glycoprotein which inhibits C1, C1r, C1s, plasma kallikrein, Factors XIa. XIIa, and plasmin. Present in plasma at 16-33 mg per 100 ml. C1 esterase inhibitor deficiency is a rare condition resulting in facial swelling and abdominal cramping. Usually the condition is hereditary, though it may also occur when the C1EI is non-functional. Prepared from plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE.

Cathepsin B, Human Liver

(HCB)

Cathepsin B, a cysteine proteinase, is located in lysosomes and is involved in tissue degradation and restructuring. Specifically, cathepsin B is believed to be involved in the intracellular digestion of extracellular proteins taken up by endocytosis. Activity: greater than 200 units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one umole of Z-Arg-Arg-beta-naphthylamide per minute at 40oC, using 100 mM Na/K phosphate, pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer. Prepared from tissue shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE.

Cathepsin D, Human Liver

(HCD)

Cathepsin D is an estrogen-regulated protein associated with tissue breakdown. Levels of cathepsin D have been positively correlated with recurring breast cancers of both node-negative and node-positive types. Additionally cathepsin D has been associated with amyloid formation in Alzheimer’s plaques. As cathepsin D activity is increased by cigarette smoke, the enzyme may contribute to lung tissue damage in smokers. Activity: >=300 units per mg protein. One unit is defined as the amount of enzyme that digests hemoglobin-releasing peptides which are soluble in 10percent TCA. The reaction is measured by an increase of an A 280 of 1.0 per 60 minutes at 37oC . Substrate: acid denatured hemoglobin, pH 1.8 (0.2percent in reaction mixture). Buffer: 100 mM formate, pH 3.3. Prepared from tissue shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE. Add deionized water to original volume, aliquot and freeze unused portion.

Human Cathepsin G

(HCG)

Cathepsin G is a serine protease purified from neutrophils that has multiple functions in the inflammatory process. Applications include substrate cleavage and inhibitor screening.

Cathepsin H, Human Liver

(HCH)

A more basic protein than Cathepsin B or L. Functions as both an aminopeptidase and endopeptidase. Cathepsin H, like cathepsin L and B, is involved in the catabolism of proteins in the lysosomal system. Activity: greater than 1 unit per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one umole of L-arginine-beta-napthylamide per minute at 40oC using 75 mM potasium phosphate, pH 6.8, with 1 mM EDTA and 3 mM cysteine as the activation buffer. Prepared from tissue shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE.

Cathepsin L, Human Liver

(HCL)

The most powerful of the lysosomal proteases. It has a higher specific activity than cathepsin B and H in the degradation of a variety of physiological protein substrates. The level of cathepsin L is a strong predictor of relapse and survival following treatment of a primary breast tumor. Activity: Greater than 1 unit per mg of protein. One unit is defined as the amount of enzyme that hydrolyzes one micromole of Z-Phe-Arg-AFC per minute at 25oC using 400 mM Na Acetate, pH 5.5 with 4 mM EDTA and 8 mM DTT as the activation buffer in the presence of Brij. Prepared from tissue shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE.

Ceruloplasmin, Human Plasma

(HCP)

An acute-phase reactant. Increased levels are associated with normal pregnancy, rheumatoid arthritis, and cirrhosis. Decreased levels are associated with hepatolenticular degeneration (Wilson’s Disease). An elevated level of Cp is found in patients with progressive tumors. Additionally, as Cp is a prooxidant, an elevated level is a sign of cardiovascular disease. Prepared from plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE. Add deionized water to original volume, aliquot and freeze unused portion.

Chymotrypsin, Human Pancreas

(HCT)

Increased values of this enzyme and/or its zymogen have been found in patients with cystic fibrosis. Activity: 40-70 units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one umole of Suc-Ala-Ala-Pro-Phe-pNA per minute at 25C, pH 8.0. Prepared from tissue shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE. Add deionized water to original volume, aliquot and freeze unused portion.

Catalase, Human Erythrocyte

(HEC)

Composed of four identical subunits each with a molecular weight of 58,000. Catalyzes the reaction 2H2O2 to H2O+O2. Catalase, along with the superoxides dismutase and glutathione peroxidase, controls the levels of oxygen-derived free radicals in mammalian cells, and together may function as a somatic oxidant defense. Prepared from whole blood shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE.

Human Fibronectin

(HFBN)

Prepared from fresh human plasma. Ideal reagent for tissue culture studies and protein-protein interactions. Thaw the fibronectin by placing the vial in a 37 C water bath and leaving it undisturbed until completely thawed. Do not disturb the vial at any time during the thawing process. If the vial is disturbed or removed prior to complete thawing, the fibronectin will form a gel and be unusable. Mix very gently with pipette after thawing. Vortexing, excessive agitation, repeated freezing and thawing of fibronectin are not recommended.

Human fibrinogen total antigen assay ELISA kit

(HFIBKT)

Fibrinogen is a soluble glycoprotein that circulates in the blood and is converted to insoluble fibrin by thrombin in the final step of the coagulation cascade. Hepatic expression of fibrinogen increases two to four hundred fold during the acute phase response to infection or inflammation. Elevated fibrinogen levels are correlated with cardiovascular disease and atherosclerosis. The sensitive quantitative measurement of total human fibrinogen antigen in plasma and serum samples is easily performed with this 96 well strip format ELISA kit. The concentration of fibrinogen in normal human plasma ranges from 1.5 to 4.5 mg/ml. The assay measures total human fibrinogen in the 3.125-800 ng/ml range. Samples giving human fibrinogen levels above 800 ng/ml should be diluted in 1X diluent before use. A 1:100,000 to 1:1,000,000 dilution for plasma or serum is suggested for best results. Human fibrinogen will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, peroxidase labeled anti-human fibrinogen primary antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of fibrinogen in the samples. A standard calibration curve is prepared using dilutions of purified fibrinogen and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, Human Fibrinogen Antigen Capture Plate, Avidin-HRP

Human Fibrinogen Antigen Capture Plate

(HFIB-TOT-PLATE)

96 well plate with 8 removable strips coated with affinity purified polyclonal antibody to human Fibrinogen at 100 ul/well and blocked with 300 ul/well. Ideal for specific binding of human fibrinogen antigen for sandwich style ELISA experiments.

Human Prothrombin

(HFII)

Human prothrombin is prepared from fresh frozen human plasma. Human Prothrombin is a glycoprotein of molecular weight 72,000, and consists of a single polypeptide chain. Activation of Prothrombin by Factor Va, Xa and phospholipids yields the serine protease Thrombin. Prothrombin purity is determined by SDS-PAGE and shows no reduction upon incubation with 2-mercaptoethanol.

Human Factor IX

(HFIX)

Human factor IX is prepared from fresh frozen plasma by a combination of conventional procedures and immunoaffinity chromatography. This protein purity is determined by SDS-PAGE and shows no reduction upon incubation with 2-mercaptoethanol. Activity is determined via clotting assay. Human Factor IX, activated by either the Contact or Tissue Factor Pathway, is responsible for the activation of Factor X to Xa.

Human Factor IXa-beta

(HFIXA)

Human Factor IXa is prepared from Human Factor IX by activation with Bovine Factor XIa. This Bovine Factor XIa is removed after activation. Complete activation is observed by SDS-PAGE. The Factor XIa activates Factor IX in a two-step reaction. In the first step, an internal Arg-Ala bond is cleaved, and in the second step, an Arg-Val bond is cleaved. The second cleavage leads to the liberation of an activation peptide from the NH2-terminal portion of the heavy chain to produce Factor IXa-beta. Factor IXa-alpha has only the second cleavage at the Arg-Val bond with about half the coagulant activity of Human Factor IXa-beta. Complete activation is observed by SDS-PAGE. This protein purity is determined by SDS-PAGE and shows total reduction upon incubation with 2-mercaptoethanol. Factor IXa-alpha has only the second cleavage at the Arg-Val bond with about half the coagulant activity of Human Factor IXa beta. Thaw rapidly in a 37C water bath without allowing protein to warm to 37C and immediately cool on ice.

Human Factor IXa, Active Site Labeled with Fluorescein

(HFIXA-FL-INFFR)

Purified Human Factor IXa is active site labeled by incubation with Fluorescein-Phe-Phe-Arg-CMK (FL-INFFR). Excess dye is removed by dialysis.

Human Factor V

(HFV)

Human factor V is prepared from fresh frozen human plasma using immunoaffinity chromatography. Purity is determined by SDS-PAGE analysis and activity is measured in a factor V clotting assay.

Human Factor Va

(HFVA)

Factor Va is prepared by activating purified factor V with thrombin and is subsequently purified by immunoaffinity chromatography. This process results in cofactor preparations which are free of both activation peptides and thrombin. Purity is determined by SDS-PAGE analysis and activity is measured in a factor Va clotting assay.

Human Factor VII

(HFVII)

Human factor VII is purified using a combination of conventional techniques and immunoaffinity chromatography. Human Factor VII is a single-chain vitamin K dependent glycoprotein found in trace quantities in plasma (0.5 mg/Liter). In the Tissue Factor Pathway of coagulation, Human Factor Vlla, in the presence of calcium ions and tissue factor, activates Factors IX and X to their enzymatically active forms, Factor IXa and Xa. Purity is determined by SDS-PAGE analysis and activity is measured in a factor VII clotting assay.

Human Factor VIIa

(HFVIIA)

Prepared from purified Human Factor VII using Human Factor XIIa. The Factor Xlla is removed using affinity chromatography. Purity is determined by SDS-PAGE. Human Factor VIIa reduces to 29,500 and 23,500 with the addition of 2-mercaptoethanol. Activity is determined via clotting assay. Factor Vlla, in the presence of calcium ions and Tissue factor, activates Factors IX and X to their enzymatically active forms, Factor IXa and Xa.

Human Factor VII total antigen assay ELISA kit

(HFVIIKT-TOT)

Factor VII is a single chain glycoprotein zymogen that initiates the extrinsic coagulation pathway by forming a complex with exposed Tissue Factor and Ca2+. Factor VII in complex is rapidly cleaved to VIIa which activates Factor X and IX. Congenital Factor VII deficiency is rare and caused by heterogeneous decreased activity or antigen levels. Elevated Factor VII is associated with increased risk of coronary heart disease. The sensitive quantitative measurement of total human Factor VII antigen in plasma samples is easily performed with this 96 well strip format ELISA kit. Factor VII, VIIa, and VII in complex with Tissue Factor will be detected by the assay. The concentration of Factor VII in normal human plasma is 470 ng/ml. The assay measures total human Factor VII in the 0.1-20 ng/ml range. Samples giving human Factor VII levels above 20 ng/ml should be diluted in blocking buffer before use. A 1:10 dilution for plasma is suggested for best results. Human Factor VII will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, anti-human Factor VII primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of Factor VII in the samples. A standard calibration curve is prepared using dilutions of purified Factor VII and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

The Human Factor VII standard provided in this kit is calibrated against the WHO International Standard for Human Plasma Factors II, VII, IX, X (NIBSC Code 09/172).

Human Factor X

(HFX)

Human factor X is isolated from fresh frozen human plasma by a combination of conventional techniques and immunoaffinity chromatography. In addition to the standard human factor X preparation, Gla-domainless human factor X is also available. Purity is determined by SDS-PAGE analysis and activity is measured in a factor X clotting assay.

Human Factor Xa

(HFXA)

Factor Xa is prepared by activating purified Factor X with the Factor X activator isolated from Russell’s viper venom. Cleavage occurs at the Arg51-Ile52 bond and releases an activation peptide. Factor Xa is purified from the activation mixture by chromatography over benzamidine-Sepharose. Purity is determined by SDS-PAGE analysis and activity is measured in a Factor Xa clotting assay and/or chromogenic substrate assay.

Human Factor Xa-beta

(HFXA-B)

Factor Xa-beta is prepared by autoproteolysis of purified Factor Xa in the presence of calcium and phospholipid followed by chromatography over immobilized benzamidine. Cleavage occurs at the Arg290-Gly291 peptide bond. Purity is determined by SDS-PAGE analysis and activity is measured in a Factor Xa clotting assay and/or chromogenic substrate assay.

Human coagulation Factor XI

(HFXI)

Human Factor XI is a two-chain glycoprotein prepared from fresh human plasma using several chromatographic steps. The two chains are identical disulfide bonded polypeptides with molecular weights of 80,000 daltons. Factor XI is activated to Factor XIa by Factor Xlla. Purity of Factor XI is >95percent by SDS-PAGE. Prepared from plasma found negative by FDA accepted methods for Anti-HIV1/2, Anti-HTLV I & II, HBsAg, Anti-HCV, Syphilis, HBC Ab, HIV-1 p24 Ag or HIV-1 RNA, HCV RNA and HBV RNA. Donors are screened for CJD (Creutzfeldt-Jakob Disease).

Human coagulation Factor XIa

(HFXIA)

Human Factor XIa is prepared from Human Factor XI using Human Factor Xlla. Factor Xlla is removed using a corn trypsin inhibitor column. Factor XI is activated to Factor XIa by Factor Xlla and High Molecular Weight Kininogen in the contact pathway of the coagulation cascade. FXI undergoes proteolytic cleavage in which the 80,000 chain reportedly is cleaved to a heavy and light chain with molecular weights of about 48,000 and 33,000. Factor XIa is responsible for the activation of Factor IX to Factor IXa. Unlike other examples of activation of Vitamin K-dependent blood-clotting proteins, Factor XIa proteolysis of Factor IX does not require membrane surfaces. Complete activation and >95percent purity is observed by SDS-PAGE. Prepared from plasma found negative by FDA accepted methods for Anti-HIV1/2, Anti-HTLV I & II, HBsAg, Anti-HCV, Syphilis, HBC Ab, HIV-1 p24 Ag or HIV-1 RNA, HCV RNA and HBV RNA. Donors are screened for CJD (Creutzfeldt-Jakob Disease).

Human coagulation Factor XII

(HFXII)

Human Factor XII is a single chain glycoprotein prepared from fresh human plasma by ion exchange chromatography. Factor XII is converted, principally from the action of kallikrein, into an active serine protease (Factor alpha-Xlla) that functions in the in vivo initiation of blood coagulation, fibrinolysis, and kinin formation. Purity of Factor XII is >95percent by SDS-PAGE and activity is determined via clotting assay. Prepared from plasma found negative by FDA accepted methods for Anti-HIV1/2, Anti-HTLV I & II, HBsAg, Anti-HCV, Syphilis, HBC Ab, HIV-1 p24 Ag or HIV-1 RNA, HCV RNA and HBV RNA. Donors are screened for CJD (Creutzfeldt-Jakob Disease).

Human coagulation Factor alpha-XIIa

(HFXIIA)

Human Factor alpha-XIIa is a serine protease responsible for the activation of Factor XI to XIa in the contact activation system. Human Factor XII and prekallikrein are thought to be involved in a reciprocal activation mechanism in which Factor XIIa activates prekallikrein to kallikrein, which in turn converts Factor XII to XIIa. Factor XIIa activates Factor XI to XIa thereby triggering the Contact Factor cascade. Human Factor alpha-XIIa is activated from homogeneous Human Factor XII by an autoactivation process with Dextran Sulfate followed by repurification to isolate Factor alpha-XIIa and Factor beta-XIIa (Cat # HFXIIAB). Complete activation is observed on SDS-PAGE. Factor XIIa is >95percent pure by SDS-PAGE and activity is determined via clotting assay. Prepared from plasma found negative by FDA accepted methods for Anti-HIV1/2, Anti-HTLV I & II, HBsAg, Anti-HCV, Syphilis, HBC Ab, HIV-1 p24 Ag or HIV-1 RNA, HCV RNA and HBV RNA. Donors are screened for CJD (Creutzfeldt-Jakob Disease).

Human coagulation Factor beta-XIIa

(HFXIIAB)

Human Factor beta-XIIa is generated in vivo by kallikrein cleavage of Factor alpha-XIIa and contains the catalytic light chain and the C-terminal peptide of the heavy chain of Factor alpha-XIIa. Human Factor beta-XIIa is activated from homogeneous Human Factor XII by an autoactivation process with Dextran Sulfate followed by repurification to isolate Factor alpha-XIIa (Cat # HFXIIA) and Factor beta-XIIa. Complete activation is observed on SDS-PAGE. Factor XIIa is >95percent pure by SDS-PAGE and activity is determined via clotting assay. Prepared from plasma found negative by FDA accepted methods for Anti-HIV1/2, Anti-HTLV I & II, HBsAg, Anti-HCV, Syphilis, HBC Ab, HIV-1 p24 Ag or HIV-1 RNA, HCV RNA and HBV RNA. Donors are screened for CJD (Creutzfeldt-Jakob Disease).

Human coagulation Factor XIII

(HFXIII)

Human Factor XIII is a tetramer composed of two pairs of chains held together by noncovalent bonds. After activation of the zymogen via Thrombin to its active enzyme form, Factor XIIIa is responsible for catalyzing the formation of covalent bridges between fibrin units to increase the elasticity of the clot network. The resulting cross-linked fibrin is very insoluble and resistant to lysis.

Human coagulation Factor XIIIa

(HFXIIIA)

Human Factor XIIIa is activated from Factor XIII by cleavage with human alpha thrombin. The thrombin is subsequently removed via chromatography. Factor XIIIa is responsible for catalyzing the formation of covalent bridges between fibrin units to increase the elasticity of the clot network. The resulting cross-linked fibrin is very insoluble and resistant to lysis.

Human Factor XI total antigen assay ELISA kit

(HFXIKT-TOT)

Factor XI is a disulfide linked two-chain glycoprotein zymogen and is the precursor of the coagulation enzyme Factor XIa. Factor XI consists of two identical monomers and circulates in plasma in complex with kininogen. Factor XI is activated by Factor XIIa and converts Factor IX to Factor IXa during the intrinsic pathway of the coagulation cascade. The sensitive quantitative measurement of total human Factor XI antigen in plasma samples is easily performed with this 96 well strip format ELISA kit. Factor XI and XIa will be detected by the assay. The concentration of Factor XI in normal human plasma ranges from 3-6 ug/ml. The assay measures total human Factor XI in the 0.2-100 ng/ml range. Samples giving human Factor XI levels above 100 ng/ml should be diluted in blocking buffer before use. A 1:1,000 dilution for plasma is suggested for best results. Human Factor XI will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, peroxidase labeled anti-human Factor XI primary antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of Factor XI in the samples. A standard calibration curve is prepared using dilutions of purified Factor XI and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

The Human Factor XI standard provided in this kit is calibrated against the WHO International Standard for Human Plasma Factor XI (NIBSC Code 04/102).

Human Factor X total antigen assay ELISA kit

(HFXKT-TOT)

Factor X is a disulfide linked two-chain glycoprotein zymogen and is the precursor of the coagulation enzyme Factor Xa. Factor X is activated by Factor IXa in complex with Factor VIII, calcium and phospholipids during the intrinsic pathway and by Factor VIIa in complex with Tissue Factor, calcium and phospholipids during the extrinsic pathway of the coagulation cascade. The sensitive quantitative measurement of total human Factor X antigen in plasma samples is easily performed with this 96 well strip format ELISA kit. Factor X, Xa, and Xa in complex with inhibitors or cofactors will be detected by the assay. The concentration of Factor X in normal human plasma is 7-8 ug/ml. The assay measures total human Factor X in the 0.1-50 ng/ml range. Samples giving human Factor X levels above 50 ng/ml should be diluted in blocking buffer before use. A 1:1,000 dilution for plasma is suggested for best results. Human Factor X will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, anti-human Factor X primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of Factor X in the samples. A standard calibration curve is prepared using dilutions of purified Factor X and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

GC Globulin, Human Plasma, Mixed Type

(HGCG)

An alpha 2 glycoprotein composed of a single polypeptide chain and present in plasma at levels of 20-55 mg/100 ml. It functions in the binding and transport of vitamin D and may also play an important role in actin homeostatis since it has been shown to bind monomeric actin with high affinity. GC-globulin also binds membranes of both circulating B and T lymphocytes. Clinically, concentrations are reduced in patients with severe liver diseases. GC-globulin may also play an important role in the mechanism of the osteomalacia that occurs with Itai-Itai disease. Prepared from plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE. Add deionized water to original volume, aliquot and freeze unused portion.

Human Glu plasminogen

(HGPG)

Prepared from fresh human plasma by immobilized lysine chromatography.

FITC Labeled Human Plasminogen

(HGPG-FITC)

FITC labeled human Glu plasminogen prepared from human plasma.

Human gamma-thrombin

(HGT)

A non-clotting derivative thrombin produced from Human alpha-Thrombin by controlled incubation with Trypsin-Sepharose. Gamma-thrombin is generated by cleavage of the B-chain at both the Arg106-Tyr107 and Lys190-Gly191 bonds. Gamma-thrombin is a noncoagulant form of thrombin that retains much of its platelet-activating capacity. Gamma-thrombin purity is determined by SDS-PAGE and protein concentration is determined by BCA assay.

Human Heparin Cofactor II

(HHCII)

Heparin Cofactor II is a single chain glycoprotein prepared from fresh frozen human plasma. HCII is a specific inhibitor of thrombin with increased activity in the presence of heparin. Heparin Cofactor II purity is determined by SDS-PAGE and the activity is measured by the ability to inhibit thrombin in the presence of saturating concentrations of heparin.

Lipoprotein, High Density, Human Plasma

(HHDL)

HDL is the vehicle for reversed cholesterol transport and estrification, serving as a scavenger for free cholesterol during intravascular catabolism of lipoproteins. HDL levels are inversely correlated with coronary heart disease. In a normal fasting individual, HDL concentrations range from 1.0-2.0 g/L. Purity: single arc by IEP against antisera to whole human serum. Essentially free of other plasma lipoproteins as determined by electrophoresis using Fat Red 7B stain for lipids and Coomassie Blue for proteins. >95percent of total lipoprotein content by electrophoresis. Prepared from fresh, non-frozen plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. Do NOT freeze this product.

Haptoglobin, Human Plasma

(HHP)

An acute-phase plasma protein found in human plasma at 100-300 mg per 100 ml. Binds hemoglobin, thus preventing loss of iron through the kidneys. Humans are polymorphic for haptoglobin, with three major phenotypes. Hp 1-1 is the most common, and the most effective in binding free hemoglobin. Hp 2-2 is the least effective. This functional difference may be associated with the frequency and severity of epilepsy attacks, as researchers have found a correlation between recurring seizures and the Hp 2-2 phenotype. Prepared from plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE. Add deionized water to original volume, aliquot and freeze unused portion. Molecular Weights by phenotype – Hp 1-1: 86,000; Hp 2-1: 200,000; Hp 2-2: 400,000.

Kininogen, Single Chain HMW, Human Plasma

(HK-HMW)

HMW kininogen, synthesized by hepatocytes, is a multifunctional protein. It is the precursor protein of bradykinin. In addition it is a nonenzymatic cofactor of the contact activation system of factor XI, XII, and prekallikrein and a major extra-cellular cysteine proteinase inhibitor. Kininogen links prekallikrein to a negatively charged surface thereby allowing activation to kallikrein by surface bound Factor alpha-Xlla. Kininogen also forms a complex with Factor XI and accelerates its activation to XIa by alpha-Xlla. Kininogens inhibit systemic proteases which are thought to have a part in various diseases such as cancer, muscular dystrophy and joint disease. >95percent pure by SDS-PAGE and >95percent single chain. Prepared from plasma found negative by FDA accepted methods for Anti-HIV1/2, Anti-HTLV I & II, HBsAg, Anti-HCV, Syphilis, HBC Ab, HIV-1 p24 Ag or HIV-1 RNA, HCV RNA and HBV RNA. Donors are screened for CJD (Creutzfeldt-Jakob Disease).

Kininogen, LMW, Human Plasma

(HK-LMW)

LMW Kininogn is involved in blood pressure regulation since it is an endogenous protein substrate for tissue kallikrein. Proteolytic cleavage by kallikrein releases vasoactive bradykinin from LMW kininogen. In addition, LMW kininogen, like HMW kininogen, is a major extracellular cysteine protease inhibitor. Prepared from plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE. Add deionized water to original volume, aliquot and freeze unused portion.

Kininogen, Two Chain HMW, Human Plasma

(HK-TC)

HMW kininogen, synthesized by hepatocytes, is a multifunctional protein. It is the precursor protein of bradykinin. In addition it is a nonenzymatic cofactor of the contact activation system of factor XI, XII, and prekallikrein and a major extra-cellular cysteine proteinase inhibitor. Kininogen links prekallikrein to a negatively charged surface thereby allowing activation to kallikrein by surface bound Factor alpha-Xlla. Kininogen also forms a complex with Factor XI and accelerates its activation to XIa by alpha-Xlla. Kininogens inhibit systemic proteases which are thought to have a part in various diseases such as cancer, muscular dystrophy and joint disease. The two chain form is prepared by kallikrein digestion of single chain kininogen. Two chain kininogen repurified to remove traces of kallikrein and is kinin free. >95percent pure by SDS-PAGE and shows complete reduction upon incubation with 2-mercaptoethanol. Prepared from plasma found negative by FDA accepted methods for Anti-HIV1/2, Anti-HTLV I & II, HBsAg, Anti-HCV, Syphilis, HBC Ab, HIV-1 p24 Ag or HIV-1 RNA, HCV RNA and HBV RNA. Donors are screened for CJD (Creutzfeldt-Jakob Disease).

Lipoprotein, Low Density, Human Plasma

(HLDL)

In a normal fasting individual, low density lipoprotein concentrations range from 2.0 – 3.5 g/L. LDL constitutes 50percent of the total lipoprotein mass in plasma and is the major carrier of cholesterol and cholesteryl esters. LDL levels strongly correlate with coronary heart disease. Purity: single arc by IEP against antisera to whole human serum. Essentially free of other plasma lipoproteins as determined by electrophoresis using Fat Red 7B stain for lipids and Coomassie Blue for proteins. >95percent of total lipoprotein content by electrophoresis. Prepared from fresh, non-frozen plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. Do NOT freeze this product.

Hamster Fibronectin

(HMFBN)

Prepared from fresh hamster plasma. Ideal reagent for tissue culture studies and protein-protein interactions. Thaw the fibronectin by placing the vial in a 37 C water bath and leaving it undisturbed until completely thawed. Do not disturb the vial at any time during the thawing process. If the vial is disturbed or removed prior to complete thawing, the fibronectin will form a gel and be unusable. Mix very gently with pipette after thawing. Vortexing, excessive agitation, repeated freezing and thawing of fibronectin are not recommended.

Human Neutrophil Elastase

(HNE)

Chromatographically purified from human leukocytes and sterile filtered. The neutrophil form of elastase is 218 amino acids long, with two asparagine-linked carbohydrate chains. No detectable amounts of Cathepsin G or MPO. Activity >10 u/ml @ 37 C. Stability > 1 year at 4 C. Aliquot and freeze at -70 C for extended storage.

Human Neutrophil Elastase, Lyophilized

(HNE-L)

Chromatographically purified from leucocytes of purulent human sputum and lyophilized. The lyophilized, salt-free, soluble powder is stable for over a year when stored at 5 C. The protein is greater than 95percent pure by SDS-PAGE and is free of cathepsin G, MPO and lysozyme. 800-900 units per mg of protein on the substrate Suc-Ala-Ala-Ala-pNA. 15000-19000 units per mg protein on the substrate MeO-Suc-Ala-Ala-Pro-Val-pNA. One unit will hydrolyze 1 umole of substrate per minute at pH 7.5 and 25 C. The following diluents and concentrations are recommended: 1) 1 mg/ml in 0.05 M Na Acetate pH 5.0 containing 0.1 M NaCl. Stable 7 days at 5 C (in ice). 2) 10 mg/ml in 50percent glycerol 50percent 0.02 M Na Acetate pH 5. Stable at least 60 days at -20 C with 20 cycles between -20 C and 5 C. 3) May be dissolved at 10 mg/ml in 2 mM HoAc and re-lyophilized in smaller quantities. Stability is better in acidic (pH 5.0) than basic buffers. Inactivation may occur at pH 8.0 due to autolysis and/or proteolysis by trypsin such as bovine pancreatic trypsin. Proteolysis can be inhibited by benzamidine. Autolysis and proteolysis can be inhibited by elastatinal. However, elastatinal is also an inhibitor of elastase. Low pH protects the elastase from both autolysis and proteolysis.

Human PAI-1 (substrate form – P12 mutant)

(HPAI-A335E)

From the laboratory of Professor Paul Declerck – Leuven BELGIUM. A single substitution at position P12 in the reactive center loop produces a PAI-1 that becomes a substrate for proteinases rather than an inhibitor. This molecule is useful for mechanistic studies.

Human PAI-1 (Neutrophil Elastase inhibitor)

(HPAI-AVI)

This human PAI-1 variant contains a mutation at the active site to make it specific for inhibition of neutrophil elastase (R346V). Additional mutations provide resistance to cleavage (V343A) and increased stability (I91L). This preparation has low endotoxin (

Human PAI-1 (point mutation stable form)

(HPAI-I91L)

This human PAI-1 mutant has a single point mutation (Isoleucine 91 to Leucine) that provides increased stability for use in long term binding experiments or in vivo studies. It is an excellent control for experiments involving the non-LRP binding stable mutant human PAI-1 (Catalog Number HPAI-R76E-I91L). The half life has not been quantified but is greater that wild type and less than the 14-1b stable mutant (Catalog Number CPAI).

Active human PAI-1 functional assay ELISA kit

(HPAIKT)

Plasminogen activator inhibitor type 1 (PAI-1) is involved in the regulation of the blood fibrinolytic system. Increased plasma levels of PAI-1 are implicated in the impairment of fibrinolytic function and may be associated with thrombotic diseases. Levels of PAI-1 increase with age and are elevated in conditions such as normal pregnancy and sepsis. The sensitive quantitative measurement of functionally active human PAI-1 in plasma samples is easily performed with this 96 well strip format ELISA kit. The PAI-1 concentration of normal platelet-free plasma is 21 ng/ml, platelet-rich plasma is 283 ng/ml and serum is 270 ng/ml. 1 PAI-1 unit = 1.34 ng. The assay measures active PAI-1 in the 0.125-100 U/ml range. Samples giving human PAI-1 levels above 100 U/ml should be diluted in PAI-1 depleted plasma before use. Normal plasma should be applied directly to the plate for best results. It is important to ensure a platelet free preparation of plasma as platelets can release PAI-1. Functionally active PAI-1 reacts with urokinase coated onto a micro titer plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected by the assay. Validated in citrate, EDTA, and heparin plasma. Vitronectin does not interfere with the detection of active PAI-1. This kit does not cross react with mouse PAI-1. After appropriate washing steps, anti human PAI-1 primary antibody binds to the PAI-1. Excess antibody is washed away, and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of PAI-1 in the samples. A standard calibration curve is prepared in PAI-1 depleted plasma using dilutions of purified PAI-1 and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

The Human PAI-1 standard provided in this kit is calibrated against the WHO International Standard for Human PAI-1 (NIBSC Code 92/654).
This assay uses an exclusive enzyme capture technology to only detect functionally active protein.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, uPA Plate, Secondary Antibody

Human PAI-1 NBD labeled at the scissile bond of the reactive loop

(HPAI-P1NBD)

P1′-NBD PAI-1 was created by mutagenesis of the methionine residue (Met347) at the P1-P1′ scissile bond to cysteine. This provides a free thiol group for labeling with NBD, a fluorescent probe highly sensitive to changes in solvation and hydrophobic environment. The fluorescence emission of P1′-NBD PAI-1 is quenched upon cleavage of the reactive center loop by a target proteinase.

Human PAI-1 NBD labeled at the reactive center loop

(HPAI-P9NBD)

P9-NBD PAI-1 was created by mutagenesis of the P9 serine residue (Ser338) on the reactive center loop to cysteine. This provides a free thiol group for incorporation of N,N’-dimethyl-N-(acetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) (NBD), a fluorescent probe highly sensitive to changes in solvation and hydrophobic environment. The fluorescence emission of P9-NBD PAI-1 is enhanced 6-7 fold upon insertion of the reactive center loop into beta-sheet A following complex formation with proteinases, formation of the latent species, or cleavage by elastase. The incorporated probe is excited at 480 nm and displays a broad emission spectrum with a peak centered 542 nm with a resultant blue-shift to 520 nm following reactive center loop insertion. The modified PAI-1 is nearly as active as wt PAI-1 and is more resistant to the spontaneous latency reaction making this an excellent tool for monitoring reaction rates of PAI-1 (1). P9-NBD PAI-1 has been utilized in a number of studies to determine the rates of loop insertion and SERPIN reaction mechanisms when reacted with various proteinases (1,2), inactivating antibodies (2) and conformational changes imposed by the binding of vitronectin (4).

References:
1. Shore JD, et al.(1995) J Biol Chem 270:5395-5398.
2. Lawrence D, et al.(2000) J Biol Chem 275:5839-5844.
3. Verhamme IM, et al.(1999) J Biol Chem 274:17511-17517.
4. Gibson A, et al.(1997) J Biol Chem 272:5112-5121.

Human PAI-1 (vitronectin reduced binding mutant)

(HPAI-Q123K)

A single mutation results in 10-fold loss of binding of the PAI-1 mutant to the important ligand vitronectin. All other aspects of PAI-1 biological activity such as anti-protease activity remain unaffected.
References
Jensen, J.K. et al. (2004) FEBS Lett. 556: 175-179.

PAI-1 mutant – stable, no LRP binding

(HPAI-R76E-I91L)

A substitution of Glutamic Acid for Arginine at position 76 greatly decreases the binding of this Human PAI-1 mutant to the low density lipoprotein receptor-related protein (LRP). The putative LRP binding exosite is thought to overlap with the heparin binding site on the PAI-1 molecule. Binding to LRP or similar receptors leads to the clearance of PAI-1 complexes. An additional mutation (Isoleucine 91 to Leucine) provides increased stability for use in long term binding experiments or in vivo studies. A human PAI-1 point mutation stable form (Catalog Number HPAI-I91L) is available as an LRP binding control PAI-1.

References:
Stefansson S. et al. (1998) J Biol Chem 273:6358-6366.

Human PAI-1 (substrate form – P12 Arginine P14 Arginine double mutant)

(HPAI-RR)

Two amino acid substitutions at positions P12 and P14 in the reactive center loop produces a PAI-1 that becomes a substrate for proteinases rather than an inhibitor. This molecule is useful for mechanistic studies.

Human PAI-1 (substrate form – P14 Arginine mutant)

(HPAI-T333R)

A single substitution at position P14 in the reactive center loop produces a PAI-1 that becomes a substrate for proteinases rather than an inhibitor. This molecule is useful for mechanistic studies.

Human PAI-1 Antigen Capture Plate

(HPAI-TOT-PLATE)

96 well plate with 8 removable strips coated with monoclonal antibody to human Plasminogen Activator Inhibitor (PAI-1) at 100 ul/well and blocked with 300 ul/well. Ideal for specific binding of human PAI-1 antigen for sandwich style ELISA experiments.

Human PAI-1 tPA complex antigen assay ELISA kit

(HPAITPAKT-COM)

Tissue-type plasminogen activator (tPA) is a serine protease that activates plasminogen to plasmin in the blood fibrinolytic system. Plasminogen activator inhibitor type 1 (PAI-1) is involved in the regulation of the blood fibrinolytic system and forms a 1:1 covalent complex with tPA and uPA. PAI-1 tPA complex increases during pregnancy, artherosclerosis, sepsis, and are associated with myocardial infarction reoccurrence. High levels of PAI-1 tPA complex in breast cancer cytosols are associated with poor survival. PAI-1 tPA complex levels may also be useful as a prognostic indicator for renal/bladder cancer, multiple organ failure, and stroke. The sensitive quantitative measurement of total human PAI-1 tPA complex antigen in plasma, serum, urine, cell culture media, or tissue extract samples is easily performed with this 96 well strip format ELISA kit. Concentration of PAI-1 tPA complex in normal human plasma was found to be 2.8 ng/ml. The assay measures PAI-1 tPA complex in the 0.5-200 ng/ml range. Samples with complex levels above 200 ng/ml should be diluted in plasma or similar fluid devoid of PAI-1 or tPA. Human tPA will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, anti-human PAI-1 primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of PAI-1 tPA complex in the samples. A standard calibration curve is prepared in PAI-1 depleted plasma using dilutions of purified PAI-1 tPA complex and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Human PAI-1 uPA complex antigen assay ELISA kit

(HPAIUPAKT-COM)

Urokinase plasminogen activator (uPA) is a serine protease that activates plasminogen to plasmin in the blood fibrinolytic system. It is also implicated in events related to cell invasion/migration. Plasminogen activator inhibitor type 1 (PAI-1) is involved in the regulation of the blood fibrinolytic system and forms a 1:1 covalent complex with tPA and uPA. The sensitive quantitative measurement of total human PAI-1 uPA complex antigen in plasma, serum, urine, cell culture media, or tissue extract samples is easily performed with this 96 well strip format ELISA kit. Concentrations of PAI-1 uPA complex in normal human plasma are low. The median concentration of complex in plasma samples from breast cancer patients was 0.068 ng/ml. Complex concentration in primary breast cancer tumor tissue extracts varied from 0.22-5.3 ng/mg total protein and were prognostic for decreased tumor size and grade and increased survival. Levels were associated with adverse grade and poor survival in a separate study. Values for lung cancer tumor tissue extracts were similar to breast cancer and varied from 0.11-5.74 ng/mg total protein. The assay measures PAI-1 uPA complex in the 0.1-100 ng/ml range. Samples with complex levels above 100 ng/ml should be diluted in plasma or similar fluid devoid of PAI-1 or uPA. Human uPA will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, anti-human PAI-1 primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of PAI-1 uPA complex in the samples. A standard calibration curve is prepared in PAI-1 depleted plasma using dilutions of purified PAI-1 uPA complex and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

tPA Specific Human PAI-1

(HPAI-YSY)

A triple mutation on the wild-type background makes this Human PAI-1 selectively inhibit tPA. Urokinase inhibition is limited to 1/25,000 of tPA inhibition by the P3Tyr-P2Ser-P1Tyr mutation.

References:
Sherman PM. et al. (1995) J Biol Chem 270:9301-9306.

Human Protein C

(HPC)

Human protein C is prepared from fresh frozen human plasma using a combination of salt precipitations and column chromatography. The protein purity is determined by SDS-PAGE and shows total reduction upon incubation with 2-mercaptoethanol. Protein C is activated to the serine protease Activated Protein C (APC) by alpha-thrombin or the complex of alpha-thrombin/thrombomodulin. APC is a potent anticoagulant through the selective inactivation of Factors Va and VIIIa.

Human Platelet Factor 4

(HPF4)

Human PF-4 is prepared from the supernatant of thrombin-activated platelets by heparin-agarose affinity chromatography. Purity is assessed by SDS-PAGE analysis and heparin-neutralizing activity is verified by clotting assay.

Human Platelet Factor 4, Recombinant

(HPF4-R)

Human PF-4 is expressed recombinantly and purified by chromatography. The purified protein in PBS is sterile filtered and lyophilized. Protein identity is confirmed by mass spectrometry (7.8 kDa monomer) and N-terminal sequencing (M-E-A-E-E). Purity is >95 percent by SDS-PAGE. Add deionized water to original volume, aliquot and freeze unused portion.

Prekallikrein, Human Plasma

(HPK)

Zymogen precursor of the plasma serine protease kallikrein. Human Prekallikrein is a single chain gamma globulin glycoprotein that participates in the early phase of contact activation, kinin formation and fibrinolysis. Prekallikrein purity is >95percent by SDS-PAGE and shows no reduction upon incubation with 2-mercaptoethanol. Prepared from plasma found negative by FDA accepted methods for Anti-HIV1/2, Anti-HTLV I & II, HBsAg, Anti-HCV, Syphilis, HBC Ab, HIV-1 p24 Ag or HIV-1 RNA, HCV RNA and HBV RNA. Donors are screened for CJD (Creutzfeldt-Jakob Disease).

Kallikrein, Human Plasma

(HPKA)

Activation of Prekallikrein with Factor alpha-XIIa produces the enzymatically active Kallikrein. Kallikrein is a serine protease which consists of a heavy chain (Mr 52kD) and light chains (Mr either 36 or 33 kD) linked by disulfide bridges. Kallikrein possesses enzymatic activity toward Factor XII, HK, Plasminogen, Factors XI, IX, and VII, prorenin and the complement system. After activation, the activating enzyme Factor XIIa is removed by affinity chromatography. Human Kallikrein purity is >95percent by SDS-PAGE and shows complete reduction upon incubation with 2-mercaptoethanol. Prepared from plasma found negative by FDA accepted methods for Anti-HIV1/2, Anti-HTLV I & II, HBsAg, Anti-HCV, Syphilis, HBC Ab, HIV-1 p24 Ag or HIV-1 RNA, HCV RNA and HBV RNA. Donors are screened for CJD (Creutzfeldt-Jakob Disease).

Human Prekallikrein total antigen assay ELISA kit

(HPKKT-TOT)

Prekallikrein is the glycosylated single chain zymogen precursor of the plasma serine protease kallikrein. Plasma prekallikrein circulates with kininogen and is activated by Factor XIIa in the intrinsic coagulation pathway. Kallikrein activates plasminogen in fibrinolysis and cleaves kininogen in the bradykinin system of vasodilation. Prekallikrein deficiency is rare and causes increased activated partial thromboplastin time. Elevated plasma prekallikrein is associated with diabetes and cardiovascular disease. The sensitive quantitative measurement of total human prekallikrein antigen in plasma samples is easily performed with this 96 well strip format ELISA kit. Kallikrein will be detected by the assay. The concentration of prekallirein in normal human plasma is 15-55 ug/ml. The assay measures human prekallikrein in the 0.02-10 ug/ml range. Samples giving human prekallikrein levels above 10 ug/ml should be diluted in blocking buffer before use. A 1:2 dilution for plasma is suggested for best results. Human prekallikrein will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, anti-human prekallikrein primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of prekallikrein in the samples. A standard calibration curve is prepared using dilutions of purified prekallikrein and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Human PAI-1 depleted plasma, sodium citrate

(HPLA-SC-PAI)

Prepared from frozen human plasma using immobilized anti-human PAI-1 IgG.

Human PAI-1/tPA double Depleted Plasma

(HPLA-SC-PAI-tPA)

Prepared from frozen human plasma using immobilized antibodies to human PAI-1 and human tPA.

Plasminogen Depleted Human Plasma (Na Citrate)

(HPLA-SC-PG)

Prepared from frozen human plasma.

Human Renin/Prorenin double Depleted Plasma

(HPLA-SC-PREN)

Prepared from frozen human plasma using immobilized anti-human Renin IgG.

Human tPA depleted plasma, sodium citrate

(HPLA-SC-tPA)

Prepared from frozen human plasma using immobilized anti-human tPA IgG.

Human IgG Depleted Serum

(HPLA-SER-GF)

Prepared from frozen human serum using immobilized Protein A.

Human plasminogen total antigen assay ELISA kit

(HPLGKT-TOT)

Plasminogen is a single chain glycoprotein zymogen and is the precursor of the fibrinolytic enzyme plasmin. Plasminogen deficiencies are classified as hypoplasminogenemia (Type I) or dysplasminogenemia (Type 2) and are associated with decreased extracellular fibrin clearance leading to mucous membrane lesions and ligneous conjunctivitis. The sensitive quantitative measurement of total human plasminogen antigen in plasma, serum, or cell culture media samples is easily performed with this 96 well strip format ELISA kit. Plasminogen, plasmin and plasmin antiplasmin complex will be detected by the assay. The concentration of plasminogen in normal human plasma is 195 ug/ml. The assay measures total human plasminogen in the 0.5-500 ng/ml range. Samples giving human plasminogen levels above 100 ng/ml should be diluted in a similar fluid depleted of plasminogen or blocking buffer before use. A 1:10,000 dilution for plasma is suggested for best results. Human plasminogen will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, anti-human plasminogen primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of plasminogen in the samples. A standard calibration curve is prepared using dilutions of purified plasminogen and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Immobilized human plasmin

(HPL-I)

We have immobilized human Lys plasmin on agarose resin by coupling of primary amines. Plasmin retains catalytic activity when coupled and may be used to efficiently activate single-chain tPA and uPA to the two-chain form (1). It may also be used to convert plasminogen from its native Glu form to the Lys form by removing the first 77 amino acids. References: 1. Wallen, P. et al. (1982) Biochim. Biophys. Acta 719:318-328.

Human Lys plasmin

(HPLM)

Prepared from Glu plasminogen by activation with immobilized human uPA. 100 percent functionally active plasmin is purified from the activation reaction by immobilized SBTI. Plasmin will undergo rapid auto proteolysis in the absence of benzamidine and should be used quickly once thawed. Aliquot to avoid freeze-thaw cycles.

Inactivated Human Plasmin

(HPLM-IN)

Prepared from Lys plasmin by active site-specific inactivation with Phe-Pro-Arg chloromethyl ketone.

Human Prorenin, Native

(HPREN)

Recombinantly produced in HEK cell culture as untagged native form prorenin and purified by affinity chromatography. Fully activatable to renin by catalytic amounts of trypsin. Prorenin is a glycosylated aspartic protease that consists of 2 homologous lobes and is the precursor of renin. Prorenin exhibits a low level of enzymatic activity relative to renin which is generated from prorenin by proteolytic cleavage of the first ~43 amino acids at the N-terminus. This so called prosegment appears to block the full enzymatic potential of the active site (1). Renin activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE, the angiotensin-converting enzyme primarily within the capillaries of the lungs. It has been reported that the levels of circulating prorenin (but not renin) are increased in diabetic subjects(2).

1) A.H. Jan Danser; Jaap Deinum ; Renin, Prorenin and the Putative (Pro)renin Receptor). Hypertension. 2005;46:1069.

2) Luetscher JA, Kraemer FB, Wilson DM, Schwartz HC, Bryer-Ash M. Increased plasma inactive renin in diabetes mellitus. A marker of microvascular complications. N Engl J Med. 1985;312:1412-1417.

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Human Prorenin, C-terminal 8x His tag

(HPREN-HIS)

Recombinantly produced in HEK cell culture and purified by chelated metal affinity chromatography. Contains a 8X-Histidine tag at C terminus for purification. Fully activatable to renin by catalytic amounts of trypsin. Prorenin is a glycosylated aspartic protease that consists of 2 homologous lobes and is the precursor of renin. Prorenin exhibits a low level of enzymatic activity relative to renin which is generated from prorenin by proteolytic cleavage of the first ~43 amino acids at the N-terminus. This so called prosegment appears to block the full enzymatic potential of the active site (1). Renin activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE, the angiotensin-converting enzyme primarily within the capillaries of the lungs. It has been reported that the levels of circulating prorenin (but not renin) are increased in diabetic subjects(2).

1) A.H. Jan Danser; Jaap Deinum ; Renin, Prorenin and the Putative (Pro)renin Receptor). Hypertension. 2005;46:1069.

2) Luetscher JA, Kraemer FB, Wilson DM, Schwartz HC, Bryer-Ash M. Increased plasma inactive renin in diabetes mellitus. A marker of microvascular complications. N Engl J Med. 1985;312:1412-1417.

Human Prorenin ELISA Kit

(HPRENKT)

The first and only commercial assay that directly measures human prorenin (Patent Pending). Prorenin is measured directly by ELISA without pretreatment of samples or conversion to renin.

Prorenin is a glycosylated aspartic protease that consists of 2 homologous lobes and is the precursor of renin. Renin activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE, the angiotensin-converting enzyme primarily within the capillaries of the lungs. It has been reported that the levels of circulating prorenin (but not renin) are increased in diabetic subjects. Plasma and serum concentrations increase in several conditions such as pregnancy, progressive diabetes mellitus, diabetes mellitus with microvascular disease, and diabetic retinopathy. The sensitive quantitative measurement of human prorenin in plasma or serum samples is easily performed with this 96 well strip format ELISA kit. Active renin will not be detected by the assay. This kit does not cross react with mouse or rat prorenin. The concentration of prorenin is 173 pg/ml in normal human plasma and 109 pg/ml in normal human serum as determined by indirect methods. The assay measures human prorenin in the 0.02-10 ng/ml range. Samples giving human prorenin levels above 10 ng/ml should be diluted in prorenin depleted plasma or blocking buffer before use. Samples of human plasma and serum may be applied directly to the plate. Human prorenin will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, anti-human prorenin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of prorenin in the samples. A standard calibration curve is prepared using dilutions of purified prorenin in depleted plasma and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

The standard curve for this kit is generated using included prorenin depleted human plasma and is best suited for measurement of prorenin in plasma or serum. This kit is also available with a standard curve in BSA/TBS for researchers measuring prorenin in culture media under catalog number HPRENKT-NP.

Human Prorenin ELISA Kit for Non Plasma Samples

(HPRENKT-NP)

The first and only commercial assay that directly measures human prorenin (Patent Pending). Prorenin is measured directly by ELISA without pretreatment of samples or conversion to renin.

Prorenin is a glycosylated aspartic protease that consists of 2 homologous lobes and is the precursor of renin. Renin activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE, the angiotensin-converting enzyme primarily within the capillaries of the lungs. It has been reported that the levels of circulating prorenin (but not renin) are increased in diabetic subjects. Plasma and serum concentrations increase in several conditions such as pregnancy, progressive diabetes mellitus, diabetes mellitus with microvascular disease, and diabetic retinopathy. The sensitive quantitative measurement of human prorenin in cell culture media or urine samples is easily performed with this 96 well strip format ELISA kit. Active renin will not be detected by the assay. This kit does not cross react with mouse or rat prorenin. The assay measures human prorenin in the 0.01-10 ng/ml range. Samples giving human prorenin levels above 10 ng/ml should be diluted in blocking buffer before use. Human prorenin will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, anti-human prorenin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of prorenin in the samples. A standard calibration curve is prepared using dilutions of purified prorenin in blocking buffer and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

The standard curve for this kit is generated using TBS/BSA and is best suited for measurement of prorenin in cell culture media or urine. This kit is also available with a standard curve in prorenin depleted human plasma for researchers measuring prorenin in plasma or serum under catalog number HPRENKT.

This assay uses a unique antibody to only detect prorenin.

Human Protein S

(HPS)

Human protein S is isolated from fresh frozen plasma by a combination of conventional methods and immunoaffinity chromatography. Protein S exists in two forms in human plasma, as the free protein and in complex with C4b-binding protein. This is the free protein from plasma which serves as a cofactor for the anticoagulant activity of activated protein C. Human protein S is a single-chain glycoprotein with >95percent purity as determined by SDS-PAGE.

Human Protein Z

(HPZ)

Human protein Z is isolated from fresh frozen plasma by a combination of precipitations and column chromatography. HPZ purity is determined by SDS-PAGE and shows no reduction upon incubation with 2-mercaptoethanol.

IgG4, Human Myeloma Plasma

(HU-IGG4M)

IgG4 antibodies will dominate the IgG response in schistosomiasis, lymphatic filariasis, and in patients after allergen immunotherapy. Unlike the other IgG subclasses, IgG4 does not activate complement. A combined IgA-IgG4 deficiency has been associated with recurrent pyogenic infections. Prepared from myeloma plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE.

IgG3, Human Plasma

(HU-IGG3)

Like IgG1, IgG3 activates complement and has an affinity for fc receptors; thus IgG1 and IgG3 are the more effective of the IgG subclasses. An IgG3 deficiency is associated with Wiscott-Aldrich disease, systemic lupus erythematosus, juvenile diabetes mellitus, and peridontal infections. IgG anti-viral antibodies are restricted to IgG subclasses 1 and 3, with IgG3 antibodies being the first to appear in response to infection. Prepared from plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE. Do NOT freeze this product.

IgG2, Human Plasma

(HU-IGG2)

IgG2 is the only IgG subclass which passes through the placenta at a level generally lower than that found in the mother. A deficiency of IgG2 indicates a poor antibody response to bacterial polysaccharides and can lead to increased susceptibility to infections caused by encapsulated bacteria. Single arc by IEP against antisera to whole human serum and human IgG. No reaction to antisera to human IgA, IgD, IgE, or IgM. Prepared from plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE.

IgG1, Human Plasma

(HU-IGG1)

In normal adult serum, the approximate percentage composition of IgG with respect to its subclasses is IgG1:65percent, Ig2:23percent, IgG3:6percent, and IgG4:6percent. Abnormal levels of one or more subclasses may be associated with autoimmune, anaphylatic and gut diseases, as well as with recurrent infection. Single arc by IEP against antisera to whole human serum and human IgG. No reaction to antisera to human IgA, IgD, IgE, or IgM. Prepared from plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE.

IgE, Human Plasma

(HU-IGE)

IgE is the least abundant immunoglobulin in plasma, found at a concentration of less that 0.6 micrograms/ml of normal plasma. Elevated IgE levels are found in patients experiencing severe allergic reactions and parasitic infections. The affinity purified IgE reacted only with anti IgE and not with anti IgG, IgA, IgM or IgD by immunodiffusion and IEP techniques. Prepared from plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE.

Albumin, Human Plasma

(HSA)

Molecular Innovations albumin is purified by proprietary chromatographic and cold ethanol fractionation methods. Heat shock albumin sold by our competitors is less pure and may contain denatured albumin.

Albumin is a water-soluble protein with considerable structural stability. It is the most abundant of the human proteins, making up 60percent of the total protein of plasma. It functions as a carrier of hormones, enzymes, fatty acids, metal ions, and medicinal products. For over fifty years, it has been used as a therapeutic agent for the restoration and maintenance of circulating blood volume for trauma, surgery and burn patients. Prepared from plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE. Add deionized water or buffer to desired volume, aliquot and freeze unused portion.

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Horse IgG, Protein G Purified

(HS-GF)

Purified from normal serum by immobilized Protein G. >95 percent pure by SDS-PAGE and preservative free.

Prealbumin, Human Plasma

(HSPA)

Serum protein found in plasma at 30 mg per 100 ml. Functions in the transport of thyroxine, vitamin A, and retinol-binding protein. Clinically, prealbumin is a sensitive indicator of impaired liver function; low levels are found in viral hepatitis, cirrhosis, malnutrition, inflammation, and surgical trauma. Prepared from plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE. Add deionized water to original volume, aliquot and freeze unused portion.

Transferrin, Human Plasma

(HTF)

Transferrin is a monomeric glycoprotein found in plasma at an average concentration of 250 mg/100 ml. The Holo form of transferrin is iron saturated and also called Siderophilin. The specific iron-binding protein in plasma, it has a role in the transportation and distribution of iron among the body organs, in iron metabolism and prevention of iron loss via the kidneys. Stored in bone marrow as Tf-bound iron, it also possesses bacteriostatic and fungistatic activity. Clinically, decreases in transferrin are observed in congenital disorders, newborns, inflammatory diseases, hypoproteinemias and nephrotic syndrome; increases are found in pregnancy, iron-deficiency anemias, and inoculation hepatitis. Transferrin is required by all types of cells in cultures for maximal growth. It is, therefore, an important transport factor used in defined culture media. Each human transferrin molecule has the ability to carry two iron ions in the ferric form (Fe3+). Dissolves in water at 10 mg per ml. Prepared from plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. Total protein determination by the Lowry method. >95percent pure and shows only one major band corresponding to the molecular weight of Transferrin by SDS-PAGE. Add deionized water to original volume, aliquot and freeze unused portion.

Hamster IgG, Protein G Purified

(HT-GF)

Purified from normal serum by immobilized Protein G. >95 percent pure by SDS-PAGE and preservative free.

Human alpha-thrombin

(HTHROM-A)

Human alpha-thrombin is prepared from purified prothrombin. Purity is determined by SDS-PAGE analysis and activity is measured in a thrombin specific clotting assay, and compared to standardized NIH thrombin.

Human tissue plasminogen activator, >85% single chain

(HTPA)

Human tissue-type plasminogen activator (tPA) is a 527 amino acid glycoprotein. Synthesized from cDNA from a human melanoma cell line. It is recombinantly produced in Chinese Hamster Ovary (CHO) cells. Fully active, >85percent single chain.

Mouse monoclonal to human tPA

(HTPA2A153)

Produces excellent western blots with free and complexed forms of tPA. Epitope localized to the heavy chain of human tPA. IgG fraction purified by immobilized Protein A. Isotype IgG.

Non-enzymatic Human tPA

(HTPA-ALA)

This human tPA has an active site serine to alanine mutation which renders it catalytically inactive. The mutation site is S478A on the mature protein and S513A on the complete mRNA sequence (UniProtKB: locus TPA_HUMAN, accession P00750). It is >90percent single chain and retains exosite binding as well as other properties of wild type tPA. Recombinantly produced in insect cells.

Reference:
Identification of a conformationally distinct form of plasminogen activator inhibitor-1, acting as a noninhibitory substrate for tissue- type plasminogen activator
PJ Declerck, M De Mol, DE Vaughan, and D Collen
J. Biol. Chem., Jun 1992; 267: 11693 – 11696.

Non-enzymatic & non-cleavable Human tPA

(HTPA-ALANC)

Human tPA with a double mutation that is permanently single chain and lacks catalytic activity. The non-catalytic mutation site is S478A on the mature protein and S513A on the complete mRNA sequence (UniProtKB: locus TPA_HUMAN, accession P00750). The non-cleavable mutation site is R275E on the mature protein and R310E on the complete mRNA sequence (UniProtKB: locus TPA_HUMAN, accession P00750). Recombinantly produced in insect cells.

Human tissue plasminogen activator, single chain, FITC labeled

(HTPA-FITC)

Fluorescein labeled tPA, >85percent single chain. 100percent complex formation with PAI-1.

Immobilized human tissue plasminogen activator, single chain

(HTPA-I)

May be used to immunopurify monoclonal and polyclonal antibodies directed against human tPA. May be used repeatedly.

Active human tPA functional assay ELISA kit

(HTPAKT)

Tissue plasminogen activator (tPA) is a serine protease that catalyzes the activation of plasminogen to plasmin. Clinical studies have indicated that high tPA levels may increase the risk for thrombosis, whereas decreased levels may cause neuronal plasticity and degeneration. The sensitive quantitative measurement of functionally active human tPA in plasma and other biological fluid samples is easily performed with this 96 well strip format ELISA kit. The basal level of tPA in healthy humans was found to be between 0.2-2 IU/ml. 1 tPA IU = 1.45 ng. The assay measures active tPA in the 0-1 IU/ml range. Samples giving human tPA levels above 1 IU/ml should be diluted in blocking buffer before use. Samples of human plasma in citrate or EDTA may be assayed with this kit. Plasma in heparin is not recommended. It is important to ensure a platelet free preparation as platelets can release PAI-1, which in turn could potentially form a complex with active tPA. If plasma samples were collected in citrate the pH should be brought up to neutral with the diluent provided in the kit. Serum and cell culture media at neutral pH may also be used. Functionally active tPA will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. Complexed tPA will not bind to the PAI-1 and will not be detected by the assay. Cross-reactivity: 3percent mouse tPA, 30percent rat tPA. After appropriate washing steps, anti-human tPA primary antibody binds to the captured enzyme. Excess antibody is washed away, and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of active tPA in the samples. A standard calibration curve is prepared using dilutions of purified tPA and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

This assay uses an exclusive enzyme capture technology to only detect functionally active protein.
The Human tPA standard provided in this kit is calibrated against the WHO International Standard for Human tPA (NIBSC Code 98/714).

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, Avidin Plate, Secondary Antibody

Human tPA total antigen assay ELISA kit

(HTPAKT-TOT)

Tissue plasminogen activator (tPA) is a serine protease that catalyzes the activation of plasminogen to plasmin. Clinical studies have indicated that high tPA levels may increase the risk for thrombosis, whereas decreased levels may cause neuronal plasticity and degeneration. The sensitive quantitative measurement of total human tPA antigen in plasma, serum, urine, cell culture media, or tissue extract samples is easily performed with this 96 well strip format ELISA kit. The basal level of tPA in healthy males and females, age 25-34 years, were found to be 5.5 ng/ml and 4.0 ng/ml respectively. The assay measures human tPA in the 0.02-10 ng/ml range. Samples giving human tPA levels above 10 ng/ml should be diluted in blocking buffer before use. Human tPA will bind to the capture antibody coated on the microtiter plate. Free and complexed tPA will be detected by the assay. This kit does not cross react with mouse or rat tPA. After appropriate washing steps, anti-human tPA primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of human tPA in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified human tPA and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, tPA Antigen Capture Plate, Secondary Antibody

Non-cleavable Human tPA

(HTPA-NC)

The addition of an arginine to glutamic acid mutation generates human tPA that is 100percent single chain and resistant to cleavage by plasmin. The mutation site is R275E on the mature protein and R310E on the complete mRNA sequence (UniProtKB: locus TPA_HUMAN, accession P00750). Recombinantly produced in insect cells.
References:
1. Functional role of proteolytic cleavage at arginine-275 of human tissue plasminogen activator as assessed by site-directed mutagenesis.
KM Tate, DL Higgins, WE Holmes, ME Winkler, HL Heyneker, and GA Vehar
Biochemistry, January 27, 1987; 26(2): 338-43.
2. Plasmin-Mediated Fibrinolysis by Variant Recombinant Tissue Plasminogen Activators
Shoko Urano, Alan R. Metzger, and Francis J. Castellino
PNAS, Apr 1989; 86: 2568 – 2571.
3. Plasminogen activation with single-chain urokinase-type plasminogen activator (scu-PA). Studies with active site mutagenized plasminogen (Ser740Ala) and plasmin-resistant scu-PA (Lys158Glu)
HR Lijnen, B Van Hoef, L Nelles, and D Collen
J. Biol. Chem., Mar 1990; 265: 5232 – 5236.

Human tissue plasminogen activator, >95% two chain

(HTPA-TC)

Activated from single-chain form with immobilized plasmin. 100percent complex formation with human PAI-1.

Human tPA Antigen Capture Plate

(HTPA-TOT-PLATE)

96 well plate with 8 removable strips coated with monoclonal antibody to human Tissue-type Plasminogen Activator (tPA) at 100 ul/well and blocked with 300 ul/well. Ideal for specific binding of human tPA antigen for sandwich style ELISA experiments.

Human tissue plasminogen activator, single chain, Texas Red

(HTPA-TR)

Texas red labeled tPA, >85percent single chain. 100percent complex formation with PAI-1.

Trypsin, Human Pancreas

(HTRYP)

Hydrolyzes peptides, amides, and ester bonds involving the carboxyl groups of L-arginine or L-lysine. Increased serum values of this enzyme and/or its zymogen (trypsinogen) have been found in patients with cystic fibrosis. Trypsinogen has three isoforms: cationic (trypsinogen 1), anionic (trypsinogen 2, MW 22,500), and mesotrypsinogen (trypsinogen 3, MW, 25,000). This trypsin is the cationic form. Activity: 2.5 units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one umole of N-benzoyl-DL-arginine-pNA per minute at 25oC in 200 mM Tris-HCl, pH 7.8, with 20 mM CaCl2. Prepared from tissue shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. Add deionized water to original volume, aliquot and freeze unused portion.

Human Thrombospondin

(HTSP)

A cellular adhesion protein. Thrombospondin is a component of the coagulation mechanism and is believed to have potential as a marker for platelet activation and renal failure. Aliquoted in siliconized tubes. Prepared from platelets shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE. Add deionized water to original volume, aliquot and freeze unused portion.

IgG, Fab Fragment, Human Plasma

(HU-FAB)

Isolated fab fragments can be used to bind antigen to antibody in solution or on the cell surface without causing the undesired complications of cross-linking and precipitation, or patching and capping, respectively. No reaction by IEP to antisera to human IgG-fc. Prepared from plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE.

IgG, Fc Fragment, Human Plasma

(HU-FC)

The IgG fc fragment does not bind antigen; however, it does contain the classic antigenic determinants and biological activity with respect to cytotrophic reactions, binding of complement and catabolic rate control. No reaction by IEP to antisera to human IgG-fab. Prepared from plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE.

IgG, Human Plasma

(HU-GF)

Purified from normal serum by immobilized Protein A. IgG is the most abundant immunoglobulin in plasma, found at a concentration of 8 to 18 mg/ml. This immunoglobulin plays a very important role in the defense against infection for newborns because of its transfer through the placenta during pregnancy. It readily diffuses into the extravascular body spaces where it plays a major role in neutralizing bacterial toxins and in enhancing the phagocytosis of microorganisms. IgG binds to bacteria and these complexes adhere to phagocytic cells which have surface receptors specific for IgG. Prepared from plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE.

IgG, Human Plasma, Low Endotoxin

(HU-GF-ED)

Purified from normal serum by immobilized Protein A using low endotoxin methodology. The purified protein is sterile filtered and lyophilized from azide fee PBS. >95 percent pure by SDS-PAGE and

IgA, Human Plasma

(HU-IGA)

IgA is the most abundant immunoglobulin in body fluids and the second most abundant immunoglobulin in plasma, found at a concentration of 0.4 to 2.2 mg/ml. It plays a very important role in the first specific defense against natural infection. Secretory IgA differs from serum IgA in that it contains two additional peptides: the secretory component and the J chain. Prepared from plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE.

IgA1, Human Myeloma Plasma

(HU-IGA1M)

In normal adult serum, the approximate percentage composition of IgA with respect to is subclasses is IgA1:90percent and IgA2:10percent. In secretory IgA, the subclass proportions may approach 50:50. The clinical significance of the subclasses has yet to be determined. No reaction by IEP to IgA2 antiserum. Prepared from myeloma plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE.

IgA2, Human Myeloma Plasma

(HU-IGA2M)

In normal adult serum, the approximate percentage composition of IgA with respect to is subclasses is IgA1:90percent and IgA2:10percent. In secretory IgA, the subclass proportions may approach 50:50. The clinical significance of the subclasses has yet to be determined. Single arc by IEP against antisera to whole human serum, human IgA2, and human kappa. Not immunoreactive to antisera against human IgA1, IgG, IgD, IgE, IgM, and lambda by IEP. Prepared from myeloma plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE.

IgD, Human Plasma

(HU-IGD)

IgD was first identified in 1965, and like other immunoglobulins, exists as both secreted and membrane forms. Its level in plasma is low with a mean concentration of 30 ug/ml. IgD is present in large quantities on the surface membrane of a majority of human circulating B lymphocytes. IgD enhances humoral immune responses through its induction of IgD-receptor expression on T lymphocytes. Ref.: Coico RF, Siskind GW, Thorbecke GJ 1988. Immunol. Rev. 105:45. Prepared from plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE.

IgD, Human Myeloma Plasma

(HU-IGDM)

IgD was first identified in 1965, and like other immunoglobulins, exists as both secreted and membrane forms. Its level in plasma is low with a mean concentration of 30 ug/ml. IgD is present in large quantities on the surface membrane of a majority of human circulating B lymphocytes. IgD enhances humoral immune responses through its induction of IgD-receptor expression on T lymphocytes. Ref.: Coico RF, Siskind GW, Thorbecke GJ 1988. Immunol. Rev. 105:45. Prepared from myeloma plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE.

Human Immunoglobulin E ELISA Kit

(HUIGEKT)

Human Immunoglobulin E (IgE) is the least abundant immunoglobulin in serum and is predominately involved in the allergy response. IgE binds to allergens and triggers histamine release from mast cells and basophils. Elevated IgE levels are found in patients experiencing severe allergic reactions and parasitic infections. The sensitive quantitative measurement of total human IgE antigen in serum, plasma, hybridoma cell supernatants, ascites or other biological fluid samples is easily performed with this 96 well strip format ELISA kit. Concentrations of 52 ng/ml in single donor plasma and 170 ng/ml in pooled plasma were found by in-house testing. 1 IU = 3.4 ng. The assay measures human IgE in the 1-500 ng/ml range. Samples giving human IgE levels above 500 ng/ml should be diluted in blocking buffer before use. A 1:10 dilution for plasma is suggested for best results. Human IgE will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, peroxidase labeled anti-human IgE primary antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of human IgE in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified human IgE and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

The human IgE standard provided in this kit is calibrated against the WHO Reference Reagent for Human Serum Immunoglobulin E (NIBSC Code 75/502).

IgG1, Human Myeloma Plasma

(HU-IGG1M)

In normal adult serum, the approximate percentage composition of IgG with respect to its subclasses is IgG1:65percent, Ig2:23percent, IgG3:6percent, and IgG4:6percent. Abnormal levels of one or more subclasses may be associated with autoimmune, anaphylatic and gut diseases, as well as with recurrent infection. Prepared from myeloma plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE.

IgG2, Human Myeloma Plasma

(HU-IGG2M)

IgG2 is the only IgG subclass which passes through the placenta at a level generally lower than that found in the mother. A deficiency of IgG2 indicates a poor antibody response to bacterial polysaccharides and can lead to increased susceptibility to infections caused by encapsulated bacteria. Prepared from myeloma plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE.

Human Immunoglobulin G ELISA Kit

(HUIGGKT)

Human Immunoglobulin G (IgG) is the most abundant immunoglobulin in serum and is predominately involved in the secondary immune response. The IgG subclasses are designated 1, 2, 3 and 4 based on their relative prevalence in human serum. The sensitive quantitative measurement of total human IgG antigen in serum, plasma, hybridoma cell supernatants, ascites or other biological fluid samples is easily performed with this 96 well strip format ELISA kit. The assay does not distinguish IgG subclasses. The concentration of IgG in normal human serum ranges from 5 to 12 mg/ml. The assay measures human IgG in the 1-500 ng/ml range. Samples giving human IgG levels above 500 ng/ml should be diluted in blocking buffer before use. A 1:500,000 dilution for serum, serially generated by 1:100, 1:100, and 1:50 dilutions, is suggested for best results. Human IgG will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-human IgG primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of human IgG in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified human IgG and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

The human IgG standard provided in this kit is calibrated against the WHO International Standard for Human Serum Immunoglobulins G, A and M (NIBSC Code 67/086).

IgM, Human Plasma

(HU-IGM)

IgM is found in normal plasma at a concentration of 1.2 to 4.0 mg/ml. It is the first immunoglobulin produced during the immune response, the first antibody in neonates, and is involved in pathogenesis of autoimmune diseases. A diminished level of IgM is associated with Wiskott-Aldrich syndrome. Prepared from plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE.

IgM, Human Myeloma Plasma

(HU-IGMM)

IgM is found in normal plasma at a concentration of 1.2 to 4.0 mg/ml. It is the first immunoglobulin produced during the immune response, the first antibody in neonates, and is involved in pathogenesis of autoimmune diseases. A diminished level of IgM is associated with Wiskott-Aldrich syndrome. Prepared from myeloma plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE.

Mouse monoclonal to human uPA, clone 3A108

(HUPA3A108)

Monoclonal antibody directed to the N-terminal alpha (light) chain region of human uPA, which includes the Amino-terminal Fragment (ATF) region. IgG fraction purified by immobilized Protein G. Isotype IgG.

Mouse monoclonal to human uPA, clone 3G65

(HUPA3G65)

Capture monoclonal antibody directed to the C-terminal beta (heavy) chain region of human uPA, which includes the active site. IgG fraction purified by immobilized Protein G. Isotype IgG.

Immobilized human urokinase

(HUPA-I)

Immobilized human two-chain HMW urokinase is ideal for the controlled activation of plasminogen to plasmin. After the activation is complete, the resin is simply removed and the reaction is quenched. May be used to immunopurify monoclonal and polyclonal antibodies directed against human urokinase. May be used repeatedly.

Active human uPA functional assay ELISA kit

(HUPAKT)

Urokinase plasminogen activator (uPA), along with its receptor uPAR, is a serine protease that activates plasminogen to plasmin in the blood fibrinolytic system. It is also implicated in events related to cell invasion and migration. Clinical studies have indicated that high uPA levels may elevate the risk for tumor invasion and metastasis. Increased expression and activity can exert potent arthritogenic properties in rheumatoid arthritis patients. Increased uPA activity may be an implication for the pathophysiology of endometriosis. The sensitive quantitative measurement of functionally active human uPA in plasma and other biological fluids is easily performed with this 96 well strip format ELISA kit. The mean value of uPA in healthy donors was found to be 1.1 ng/ml. The assay measures active uPA in the 0.1-50 ng/ml range. Samples giving human uPA levels above 50 ng/ml should be diluted in blocking buffer before use. Samples of human plasma in citrate or EDTA may be assayed with this kit. Plasma in heparin is not recommended. It is important to ensure a platelet free preparation as platelets can release PAI-1, which in turn could potentially form a complex with active uPA. Serum and cell culture media at neutral pH may also be used. Functionally active uPA will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. Inactive or complexed uPA will not bind to the PAI-1 and will not be detected by the assay. This kit does not cross react with mouse uPA. After appropriate washing steps, anti-human uPA primary antibody binds to the captured enzyme. Excess antibody is washed away, and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of active uPA in the samples. A standard calibration curve is prepared using dilutions of purified uPA and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

This assay uses an exclusive enzyme capture technology to only detect functionally active protein.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, Avidin Plate, Secondary Antibody

Human uPA total antigen assay ELISA kit

(HUPAKT-TOT)

Urokinase plasminogen activator (uPA), along with its receptor uPAR, is a serine protease that activates plasminogen to plasmin in the blood fibrinolytic system. It is also implicated in events related to cell invasion and migration. Clinical studies have indicated that high uPA levels may elevate the risk for tumor invasion and metastasis. Increased expression and activity can exert potent arthritogenic properties in rheumatoid arthritis patients. Increased uPA activity may be an implication for the pathophysiology of endometriosis. The sensitive quantitative measurement of total human uPA antigen in plasma, serum, culture media or tissue extract samples is easily performed with this 96 well strip format ELISA kit. The concentration of uPA antigen in human plasma has been reported to be 3.5 ng/ml. The assay measures total uPA in the 0.1-50 ng/ml range. Samples giving human uPA levels above 50 ng/ml should be diluted in blocking buffer before use. Human uPA will bind to the capture antibody coated on the microtiter plate. Free and complexed uPA will be detected by the assay. After appropriate washing steps, anti-human uPA primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of human uPA in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified human uPA and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Lipoprotein, Very Low Density, Human Plasma

(HVLDL)

In a normal fasting individual, VLDL concentrations range from 0.5 – 2.0 g/L. VLDL transports triglycerides synthesized by the liver to sites of energy storage and utilization. Purity: single arc by IEP against antisera to whole human serum. Essentially free of other plasma lipoproteins as determined by electrophoresis using Fat Red 7B stain for lipids and Coomassie Blue for proteins. >95percent of total lipoprotein content by electrophoresis. Prepared from fresh, non-frozen plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. Do NOT freeze this product.

Human monomeric vitronectin

(HVN)

Prepared from fresh human plasma using non-denaturing chromatography.

Inhibitory mouse monoclonal to human vitronectin

(HVN1D144)

Inhibitory monoclonal antibody produced in mouse. This antibody blocks smooth muscle and endothelial cell adhesion to vitronectin. Does not cross-react with mouse. IgG fraction purified by immobilized Protein A.

Mouse monoclonal to human vitronectin

(HVN1E934)

ELISA: Monomeric and multimeric human vitronectin
WB: Non-reduced monomeric vitronectin
Monoclonal antibody produced in mouse. Does not cross-react with mouse. IgG fraction purified by immobilized Protein A.

Mouse monoclonal to human vitronectin

(HVN1H820)

ELISA: Monomeric and multimeric human vitronectin
WB: Non-reduced monomeric vitronectin
Monoclonal antibody produced in mouse. Does not cross-react with mouse. IgG fraction purified by immobilized Protein A.

Mouse monoclonal to human vitronectin

(HVN2C323)

WB: Reduced and non-reduced monomeric vitronectin
ELISA: Monomeric vitronectin only
Monoclonal antibody produced in mouse. Does not cross-react with mouse. IgG fraction purified by immobilized Protein A.

Mouse monoclonal to human vitronectin

(HVN4A132)

WB: Reduced and non-reduced monomeric vitronectin
ELISA: Not suitable
Monoclonal antibody produced in mouse. Does not cross-react with mouse. IgG fraction purified by immobilized Protein A.

Biotin labeled human monomeric vitronectin

(HVN-BIO)

Prepared from fresh human plasma using non-denaturing chromatography, then biotin labeled at primary amines.

Human vitronectin total antigen assay ELISA kit

(HVNKT-TOT)

Vitronectin is an abundant plasma glycoprotein that helps regulate coagulation, fibrinolysis, complement activation, and cell adhesion. Vitronectin binds to glycosaminoglycans, collagen, plasminogen and urokinase receptors. It also may control the clearance of vascular thrombi by binding and stablilizing PAI-1. In binding PAI-1, it extends the lifetime of active PAI-1. Vitronectin may also be involved in the regulation of bone metabolism. The sensitive quantitative measurement of total human vitronectin antigen in plasma, serum, culture media or tissue extract samples is easily performed with this 96 well strip format ELISA kit. Using this assay, values for plasma vitronectin in normal individuals were determined to be 127.5 +/- 13.1 ug/ml. Vascular samples from the saphenous vein, mammary artery, and human adipose tissue were also assayed for vitronectin, with values ranging from 5-40 ug/mg. The assay measures total vitronectin in the 0.05-100 ng/ml range. Samples giving human vitronectin levels above 100 ng/ml should be diluted in blocking buffer before use. Human vitronectin will bind to the capture antibody coated on the microtiter plate. This kit does not cross react with mouse vitronectin.After appropriate washing steps, anti-human vitronectin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of human vitronectin in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified human vitronectin and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, Human Vitronectin Antigen Capture Plate, Secondary Antibody

Human Vitronectin Antigen Capture Plate

(HVN-TOT-PLATE)

96 well plate with 8 removable strips coated with monoclonal antibody to human Vitronectin at 100 ul/well and blocked with 300 ul/well. Ideal for specific binding of human vitronectin antigen for sandwich style ELISA experiments.

Human multimeric vitronectin

(HVN-U)

Prepared from fresh human plasma using urea as a denaturant.

Biotin labeled human multimeric vitronectin

(HVN-U-BIO)

Prepared from fresh human plasma using urea as a denaturant, then biotin labeled at primary amines.

Human von Willebrand Factor

(HVWF)

vWF is prepared from citrated human plasma using a combination of chromotographic procedures. Although the starting material was tested prior to initiation of the manufacturing process, and was found negative or nonreactive for anti-HIV-1/2, HIV-1 antigen(s), HBsAg, STS, anti-HCV, anti-HBcore and anti-HTLV I & II, extreme caution should be used when handling this material as there is a margin of error in all tests. This preparation is >95percent pure as judged by SDS-PAGE under reducing conditions, and consists of large multimers as determined by electrophoresis in SDS/agarose gels. Molecular weight under native conditions ranges from 520 kDa (dimer) to >10,000 kDa (multimers). Protein concentration determined by total protein assay. Required thawing procedure: Thaw in a 37 degree celsius water bath for 2-3 minutes without agitation.

ATA-Phe-Phe-Arg-CMK

(INFFRCK)

ATA-FFRCK (Na-[(acetylthio) acetyl]-D-Phe-Phe-Arg-CH2Cl) and ATA-FPRCK (Na-[(acetylthio)acetyl]-D-Phe-Pro-Arg-CHCl2) are active-site specific labeling reagents for serine proteases. They are derivatized from irreversible peptide chloromethyl ketones and facilitate incorporation of a thioester moiety via alkylation of the catalytic site Histidine residue. Liberation of the free thiol group is accomplished by gentle treatment with hydroxylamine (NH2OH) following irreversible incorporation into the enzyme catalytic site. The free thiol then becomes a site for specific modifications with thiol-reactive probes such as iodoacetamide fluorescent probes. Many serine proteases in which free thiols are lacking may be specifically labeled at the active site by these reagents. Both ATA-FPRCK and ATA-FFRCK have been used to label thrombin with 5-(iodoacetamido) fluorescein (5-IAF). The probe was then effectively utilized to follow conformational changes in the catalytic domain of alpha-thrombin upon binding to the fragment 2 domain of prothrombin. In addition, quantitative equilibrium binding studies and investigations into the kinetics underlying the non-proteolytic activation of the zymogen plasminogen by streptokinase were characterized with 2-((4′-iodoacetamido) anilino) naphthalene-6-sulfonic acid (IAANS) labeled plasminogen by using the ATA-FFRCK reagent. References: 1. Bock, P. (1993) Method Enzymol. 222:478-503. 2. Bock, P. et al. (1996) J Biol. Chem. 271:1072-1080.

ATA-Phe-Pro-Arg-CMK

(INFPRCK)

ATA-FFRCK (Na-[(acetylthio) acetyl]-D-Phe-Phe-Arg-CH2Cl) and ATA-FPRCK (Na-[(acetylthio)acetyl]-D-Phe-Pro-Arg-CHCl2) are active-site specific labeling reagents for serine proteases. They are derivatized from irreversible peptide chloromethyl ketones and facilitate incorporation of a thioester moiety via alkylation of the catalytic site Histidine residue. Liberation of the free thiol group is accomplished by gentle treatment with hydroxylamine (NH2OH) following irreversible incorporation into the enzyme catalytic site. The free thiol then becomes a site for specific modifications with thiol-reactive probes such as iodoacetamide fluorescent probes. Many serine proteases in which free thiols are lacking may be specifically labeled at the active site by these reagents. Both ATA-FPRCK and ATA-FFRCK have been used to label thrombin with 5-(iodoacetamido) fluorescein (5-IAF). The probe was then effectively utilized to follow conformational changes in the catalytic domain of alpha-thrombin upon binding to the fragment 2 domain of prothrombin. In addition, quantitative equilibrium binding studies and investigations into the kinetics underlying the non-proteolytic activation of the zymogen plasminogen by streptokinase were characterized with 2-((4′-iodoacetamido) anilino) naphthalene-6-sulfonic acid (IAANS) labeled plasminogen by using the ATA-FFRCK reagent. References: 1. Bock, P. (1993) Method Enzymol. 222:478-503. 2. Bock, P. et al. (1996) J Biol. Chem. 271:1072-1080.

Inhibitory mouse monoclonal to rat PAI-1

(MA-124K1)

Inhibitory monoclonal antibody to rat PAI-1. It has recently been reported that binding of this antibody to rat PAI-1 inhibits rat PAI-1 activity and simultaneously increases the binding of inactive PAI-1 to vitronectin. Can be used as a capture antibody in ELISA assays. Purified by immobilized Protein A. IgG1k class.

Mouse monoclonal to human PAI-1

(MA-14D5)

Monoclonal antibody binds to an epitope (neoantigenic) that forms after PAI-1 has formed a complex with target proteinases. Purified by immobilized Protein A. IgG1k class.

Anti Human VLDL receptor, clone 1H10

(MA-1H10)

Monoclonal antibody produced in mouse. Useful for ELISA and western blot under non-reducing conditions. Binds to an epitope on the C-terminus of VLDL receptor ligand binding domain (amino acids 191-355, UniProtKB: locus VLDLR_HUMAN, accession P98155) and blocks apoE4 binding. Purified by immobilized Protein A. IgG1 class.
References:
Functional domains of the very low density lipoprotein receptor: molecular analysis of ligand binding and acid-dependent ligand dissociation mechanisms. Mikhailenko I et al. Journal of Cell Science 112, 3269-3281 (1999).
The apoE isoform binding properties of the VLDL receptor reveal marked differences from LRP and the LDL receptor. Ruiz J et al. Journal of Lipid Research 46, 1721-1731 (2005).

Anti Human VLDL receptor, clone 1H5

(MA-1H5)

Monoclonal antibody produced in mouse. Useful for ELISA. Binds to an epitope on the C-terminus of VLDL receptor ligand binding domain (amino acids 191-355, UniProtKB: locus VLDLR_HUMAN, accession P98155) and blocks apoE4 binding. Purified by immobilized Protein A. IgG1 class.
References:
Functional domains of the very low density lipoprotein receptor: molecular analysis of ligand binding and acid-dependent ligand dissociation mechanisms. Mikhailenko I et al. Journal of Cell Science 112, 3269-3281 (1999).
The apoE isoform binding properties of the VLDL receptor reveal marked differences from LRP and the LDL receptor. Ruiz J et al. Journal of Lipid Research 46, 1721-1731 (2005).

Mouse alpha 2 antiplasmin, Flag tag

(MA2AP-FLAG)

Recombinantly produced in insect cells and purified by FLAG affinity. Contains a Flag tag (DYKDDDDK) at N terminus for purification.

Mouse alpha 2 antiplasmin, His tag

(MA2AP-HIS)

Recombinantly produced in insect cells and purified by chelated metal affinity chromatography. Contains a 6X-Histidine tag at N terminus for purification.

Mouse antiplasmin total antigen assay ELISA kit

(MA2APKT-TOT)

Alpha-2-antiplasmin is the major circulating inhibitor of plasmin. It plays a role in the regulation of intravascular fibrinolysis. Decreased levels of alpha-2-antiplasmin may play an important role in the increased capacity of the fibrinolytic function and may be beneficial in the treatment of thrombotic diseases, acute pulmonary embolism, and hepatic repair. The sensitive quantitative measurement of total mouse alpha-2-antiplasmin antigen in plasma or serum samples is easily performed with this 96 well strip format ELISA kit. The normal mouse concentration of antiplasmin is 86 ug/ml in plasma. The assay measures total antiplasmin in the 0.1-100 ng/ml range. Samples giving mouse antiplasmin levels above 100 ng/ml should be diluted in blocking buffer before use. A 1:10,000-20,000 dilution for plasma is suggested for best results. Mouse antiplasmin will bind to the capture antibody coated onto a micro titer plate. Free and complexed antiplasmin will bind to the plate and will be detected by the assay. After appropriate washing steps, biotin labeled anti-mouse antiplasmin primary antibody binds to the antiplasmin. Excess antibody is washed away, and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of antiplasmin in the samples. A standard calibration curve is prepared using dilutions of purified antiplasmin and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Mouse monoclonal to human PAI-1

(MA-31C9)

Non-inhibitory monoclonal antibody to human PAI-1 which does not affect binding with vitronectin. Can be used as a capture antibody in ELISA assays. Purified by immobilized Protein A. IgG1k class.

Mouse monoclonal to rat PAI-1

(MA-32K3)

Non-inhibitory monoclonal antibody to rat PAI-1. Purified by immobilized Protein A. IgG1k class.

Inhibitory mouse monoclonal to human PAI-1 clone 33B8

(MA-33B8)

Inhibitory monoclonal antibody to human and rabbit PAI-1. It binds to a conformational epitope on PAI-1 converting it into the latent form. Currently being studied as a potential therapeutic agent in animal studies. Purified by immobilized Protein A. IgG1k class.

Inhibitory mouse monoclonal to human PAI-1

(MA-33H1F7)

Inhibitory monoclonal antibody to human and mouse PAI-1. It binds to an epitope localized to the F-Helix of PAI-1. The PAI-1/mab complex becomes a substrate rather than an inhibitor of its target proteinases tPA and urokinase. Currently being studied as a potential therapeutic agent in animal models. Purified by immobilized Protein A. IgG1 class.

Mouse monoclonal to human 85 kDa LRP, Clone 5A6

(MA-5A6)

Monoclonal antibody to human 85 kDa light chain of LRP. Cross-reacts with rabbit and mouse LRP. Stimulates cellular-mediated uptake of alpha 2 macroglobulin trypsin complex by LRP1. Use WI-38 fibroblast as positive control and human umbilical vein endothelial cells (HUVEC) as negative control. Purified by immobilized Protein A. IgG2b kappa class.

Biotin labeled Anti Human 85 kDa LRP, Clone 5A6

(MA-5A6-BIO)

Biotin labeled monoclonal antibody to human 85 kDa light chain of LRP. Purified by immobilized Protein A. IgG2b kappa class.

FITC labeled Anti Human 85 kDa LRP, Clone 5A6

(MA-5A6-FITC)

Fluorescein labeled monoclonal antibody to human 85 kDa light chain of LRP. Purified by immobilized Protein A. IgG2b kappa class.

Anti Human VLDL receptor, clone 5F3

(MA-5F3)

Monoclonal antibody produced in mouse. Useful for ELISA and western blot under reducing and non-reducing conditions. Binds to an epitope on the VLDL receptor ligand binding domain (amino acids 31-355, UniProtKB: locus VLDLR_HUMAN, accession P98155). Purified by immobilized Protein A. IgG1 class.
References:
Functional domains of the very low density lipoprotein receptor: molecular analysis of ligand binding and acid-dependent ligand dissociation mechanisms. Mikhailenko I et al. Journal of Cell Science 112, 3269-3281 (1999).
The apoE isoform binding properties of the VLDL receptor reveal marked differences from LRP and the LDL receptor. Ruiz J et al. Journal of Lipid Research 46, 1721-1731 (2005).

Mouse monoclonal to human RAP

(MA-7F1)

Monoclonal antibody to human RAP. No cross-reaction with rabbit or mouse RAP. IgG fraction purified by immobilized Protein A. Isotype IgG1.

Biotin labeled Anti Human RAP

(MA-7F1-BIO)

Biotin labeled monoclonal antibody to human RAP. Purified by immobilized Protein A. IgG1 class.

FITC labeled Anti Human RAP

(MA-7F1-FITC)

Fluorescein labeled monoclonal antibody to human RAP. Purified by immobilized Protein A. IgG1 class.

Mouse monoclonal to human 85 kDa LRP, Clone 8B8

(MA-8B8)

Monoclonal antibody to human 85 kDa light chain of LRP. Cross-reacts with rabbit LRP. Use WI-38 fibroblast as positive control and human umbilical vein endothelial cells (HUVEC) as negative control. Purified by immobilized Protein A. IgG1 class.

Biotin labeled Anti Human 85 kDa LRP, Clone 8B8

(MA-8B8-BIO)

Biotin labeled monoclonal antibody to human 85 kDa light chain of LRP. Purified by immobilized Protein A. IgG1 class.

FITC labeled Anti Human 85 kDa LRP, Clone 8B8

(MA-8B8-FITC)

Fluorescein labeled monoclonal antibody to human 85 kDa light chain of LRP. Purified by immobilized Protein A. IgG1 class.

Mouse monoclonal to human 515 kDa LRP

(MA-8G1)

Monoclonal antibody to human 515 kDa heavy chain of LRP. Useful marker for the characterization of a subset of myelo-monocytic subtypes of chronic and acute leukemia (CD91). Blocks cellular-mediated uptake of alpha 2 macroglobulin trypsin complex by LRP1. Use WI-38 fibroblast as positive control and human umbilical vein endothelial cells (HUVEC) as negative control. Purified by immobilized Protein A. IgG1kappa class.

Biotin labeled Anti Human 515 kDa LRP

(MA-8G1-BIO)

Biotin labeled monoclonal antibody to human 515 kDa heavy chain of LRP. Purified by immobilized Protein A. IgG1k class.

FITC labeled Anti Human 515 kDa LRP

(MA-8G1-FITC)

Fluorescein labeled monoclonal antibody to human 515 kDa heavy chain of LRP. Purified by immobilized Protein A. IgG1k class.

Anti-Human Prothrombin

(MA-HFII)

Clone number AHP-5013. Reactive to: Human prothrombin, prethrombin-1, fragment 2, meizothrombin. Does not recognize mouse thrombin by western blot. Inhibits clotting and prothrombin activation. Clotting inhibition was determined by incubating the antibody with human normal pooled plasma followed by a PT assay. Extension of the clot time reflects inhibition of the clotting. The inhibition of the prothrombin activation was determined in prothrombinase reactions using purified clotting factors and DAPA as a substrate for thrombin. Mouse monoclonal of isotype IgG2a.

Anti-Human Protein C, Clone 1

(MA-HPC-1)

Clone number AHPC-5071. Recognizes human protein C and activated protein C. Partial calcium dependence. Mouse monoclonal of isotype IgG1.

Anti-Human Protein C, Clone 2

(MA-HPC-2)

Clone number AHPC-5072. Recognizes human protein C. Mouse monoclonal of isotype IgG2b.

Anti-Human Protein S

(MA-HPS-2)

Clone number AHPS-5092. Reactive to: Human protein S. Mouse monoclonal of isotype IgG1.

Anti-Human TAFI, Clone 1

(MA-HTAFI-1)

Clone number AHTAFI-5024. Reactive to: Human TAFI, activated TAFI. Mouse monoclonal of isotype IgG1.

Anti-Human TAFI, CLone 2

(MA-HTAFI-2)

Clone number AHTAFI-5026. Reactive to: Human TAFI, activated TAFI. Mouse monoclonal of isotype IgG1.

Anti-Human TAFI, Clone 3

(MA-HTAFI-3)

Clone number AHTAFI-5065. Reactive to: Human TAFI, activated TAFI. Mouse monoclonal of isotype IgG3.

Anti-Human TAFI, Clone 4

(MA-HTAFI-4)

Clone number AHTAFI-5081. Reactive to: Human TAFI. Mouse monoclonal of isotype IgG2b.

Anti-Human Tissue Factor

(MA-HTF)

Clone number AHTF-5264. Reactive to: Human Tissue Factor. Does not react with mouse Tissue Factor. Mouse monoclonal of isotype IgG.

Anti-Human Tissue Factor Pathway Inhibitor

(MA-HTFPI)

Clone number AHTFPI-5138. Reactive to: Human Tissue Factor Pathway Inhibitor. Mouse monoclonal of isotype IgG.

Anti-Human Thrombin

(MA-HTHROM)

Clone number AHT-5020. Reactive to: Human thrombin, thrombin-ATIII complex, thrombin-PPACK. Mouse monoclonal of isotype IgG1.

Anti-Mouse Factor IX, Clone 1

(MA-MFIX-1)

Clone number AMIXA-9041. Detects Factor IX and Factor IXa in ELISA, Factor IX and Factor IXa heavy chain reduced and non-reduced in western blot, does not cross react with human Factor IX and Factor IXa. Host rat.

Anti-Mouse Factor IX, Clone 2

(MA-MFIX-2)

Clone number AMIXA-9042. Detects Factor IX and Factor IXa in ELISA, Factor IX and Factor IXa non-reduced in western blot, does not cross react with human Factor IX and Factor IXa. Host rat.

Anti-Mouse Factor VII

(MA-MFVII)

Clone number AMVII-9031. Detects recombinant mouse Factor VII and Factor VIIa in ELISA, recombinant mouse Factor VII and Factor VIIa non-reduced in western blot, does not cross react with human Factor VII and Factor VIIa. Host rat.

Anti-Mouse Factor VIII

(MA-MFVIII)

Clone number AMVIII-9035. Detects recombinant mouse Factor VIII in ELISA and recombinant mouse Factor VIII non-reduced in western blot. Native mouse and human Factor VIII have not been tested. Host rat.

Anti-Mouse Factor X, Clone 1

(MA-MFX-1)

Clone number AMX-9050. Detects Factor X and Factor Xa in ELISA, Factor X and Factor Xa heavy chain reduced and non-reduced in western blot, cross reacts with human Factor X and Factor Xa. Host rat.

Anti-Mouse Factor X, Clone 2

(MA-MFX-2)

Clone number AMX-9051. Detects Factor X in ELISA, Factor X heavy chain non-reduced in western blot. Host rat.

Anti-Mouse Protein C, Clone 1

(MA-MPC-1)

Clone number AMPC-9071. Detects Protein C in ELISA, Protein C reduced and non-reduced in western blot, inhibits Protein C activation. Host rat.

Anti-Mouse Protein C, Clone 2

(MA-MPC-2)

Clone number AMPC-9072. Detects Protein C and APC in ELISA, Protein C reduced and non-reduced in western blot. Host rat.

Anti-Mouse Plasminogen

(MA-MPG)

Clone number AMPG-9130. Detects plasminogen and plasmin in ELISA, plasmin non-reduced and plasminogen reduced and non-reduced in western blot. Host rat.

Anti-Mouse Prothrombin

(MA-MPT)

Clone number AMP-9013. Detects prothrombin in ELISA, prothrombin non-reduced in western blot, does not cross react with thrombin. Host rat.

Mouse monclonal to mouse antiplasmin

(MAP25C3)

Non-inhibitory monoclonal antibody produced in mouse. IgG fraction purified by immobilized Protein A. Isotype IgG.

Inhibitory mouse monclonal to mouse antiplasmin clone 27C9

(MAP27C9)

Inhibitory monoclonal antibody produced in mouse. IgG fraction purified by immobilized Protein A. Isotype IgG.

Inhibitory mouse monclonal to mouse antiplasmin clone 4H9

(MAP4H9)

Inhibitory monoclonal antibody produced in mouse. IgG3K fraction purified by immobilized Protein A. Isotype IgG3 kappa.

Amino terminal fragment of Mouse uPA

(MATF)

Amino terminal fragment of mouse urokinase.

Amino terminal fragment of Mouse uPA, Alexa Fluor 750 Labeled

(MATF-AF750)

Amino terminal fragment of mouse urokinase. Labeled with Alexa Fluor 750 at primary amines (Abs: 753, Em: 782).

Mouse Antithrombin III

(MATIII)

Prepared from fresh mouse plasma using several chromatographic steps.

Mouse Fibrinogen

(MFBGN)

Prepared from fresh mouse plasma using several chromatographic steps. Plasminogen depleted by lysine affinity chromatography. Thaw protein in a 37C water bath. Aliquot and freeze the unused portion. Keep fibrinogen at 25-37C, aliquot and flash freeze unused portion.

FITC Labeled Mouse Fibrinogen

(MFBGN-FITC)

Mouse fibrinogen amino terminal labeled with fluorescein isothiocyanate (FITC). Prepared from fresh mouse plasma using several chromatographic steps. Plasminogen depleted by lysine affinity chromatography. Thaw at 37C without agitation until completely liquid, then gently mix before use. Keep fibrinogen at 25-37C, aliquot and flash freeze unused portion.

Mouse fibrinogen total antigen assay ELISA kit

(MFBGNKT)

Fibrinogen is a soluble glycoprotein that circulates in the blood and is converted to insoluble fibrin by thrombin in the final step of the coagulation cascade. Hepatic expression of fibrinogen increases two to four hundred fold during the acute phase response to infection or inflammation. Elevated fibrinogen levels are correlated with cardiovascular disease and atherosclerosis. The sensitive quantitative measurement of total mouse fibrinogen antigen in plasma and serum samples is easily performed with this 96 well strip format ELISA kit. The concentration of fibrinogen in normal mouse plasma ranges from 1.4-2.1 mg/ml and varies by strain and diet. The assay measures total mouse fibrinogen in the 3.125-800 ng/ml range. Samples giving mouse fibrinogen levels above 800 ng/ml should be diluted in 1X diluent before use. A 1:100,000 to 1:1,000,000 dilution for plasma or serum is suggested for best results. Mouse fibrinogen will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-mouse fibrinogen primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of fibrinogen in the samples. A standard calibration curve is prepared using dilutions of purified fibrinogen and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, Mouse Fibrinogen Antigen Capture Plate, Avidin-HRP

Mouse Fibrinogen Antigen Capture Plate

(MFBGN-TOT-PLATE)

96 well plate with 8 removable strips coated with affinity purified polyclonal antibody to mouse Fibrinogen at 100 ul/well and blocked with 300 ul/well. Ideal for specific binding of mouse fibrinogen antigen for sandwich style ELISA experiments. Cross reacts with rat fibrinogen.

Mouse Fibronectin

(MFBN)

Prepared from fresh mouse plasma. Ideal reagent for tissue culture studies and protein-protein interactions. Thaw the fibronectin by placing the vial in a 37 C water bath and leaving it undisturbed until completely thawed. Do not disturb the vial at any time during the thawing process. If the vial is disturbed or removed prior to complete thawing, the fibronectin will form a gel and be unusable. Mix very gently with pipette after thawing. Vortexing, excessive agitation, repeated freezing and thawing of fibronectin are not recommended.

Mouse Prothrombin

(MFII)

Mouse prothrombin is prepared from fresh frozen mouse plasma. Purity is determined by SDS-PAGE analysis.

Mouse Factor IX

(MFIX)

Factor IX is prepared from fresh frozen plasma by a combination of conventional procedures and immunoaffinity chromatography. Purity is determined by SDS-PAGE analysis and activity is measured using a factor IX clotting assay.

Mouse Factor IXa

(MFIXA)

Factor IXa is prepared from highly purified factor IX by activation with factor XIa. The factor IXa is further purified by gel filtration, followed by immunoaffinity purification. Purity is assessed by SDS-PAGE analysis. Activity is determined in a one-stage clotting assay.

Mouse Factor X

(MFX)

Factor X is isolated from fresh frozen plasma by a combination of conventional techniques and immunoaffinity chromatography. Purity is determined by SDS-PAGE analysis and activity is measured in a factor X clotting assay.

Mouse Factor Xa

(MFXA)

Factor Xa is prepared by activating purified factor X with the factor X activator isolated from Russell’s viper venom. Factor Xa is purified from the activation mixture by chromatography over benzamidine-Sepharose. Purity is determined by SDS-PAGE analysis and activity is measured in a factor Xa clotting assay and/or chromogenic substrate assay.

Mouse Factor X, C-terminal 8x His tag

(MFX-HIS)

Recombinantly produced in HEK cell culture and purified by conventional and chelated metal affinity chromatography. Contains a 8X-Histidine tag at C terminus for purification. Fully activatable to FXa by Russell’s Viper Venom. Complements for Factor X in human deficient plasma clotting assays. Concentration is determined by absorbance at 280 nm using an extinction coefficient of 0.953 mg-1 ml-1.

Mouse Factor X total antigen assay ELISA kit

(MFXKT-TOT)

Factor X is a disulfide linked two-chain glycoprotein zymogen and is the precursor of the coagulation enzyme Factor Xa. Factor X is activated by Factor IXa in complex with Factor VIII, calcium and phospholipids during the intrinsic pathway and by Factor VIIa in complex with Tissue Factor, calcium and phospholipids during the extrinsic pathway of the coagulation cascade. The sensitive quantitative measurement of total mouse Factor X antigen in citrated plasma, serum, cell culture media, or tissue extract samples is easily performed with this 96 well strip format ELISA kit. Factor X, Xa, and Xa in complex with inhibitors or cofactors will be detected by the assay. The concentration of Factor X in normal plasma is 10 ug/ml. The assay measures total mouse Factor X in the 2.5-1000 ng/ml range. Samples giving mouse Factor X levels above 1000 ng/ml should be diluted in blocking buffer before use. A 1:100 dilution for plasma is suggested for best results. Mouse Factor X will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-mouse Factor X primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of Factor X in the samples. A standard calibration curve is prepared using dilutions of purified Factor X and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Phe-Pro-Arg-Chloromethylketone

(PPACK)

Phe-Pro-Arg-CMK (FPRCK). Extremely potent and selective irreversible inhibitor of thrombin (Kobs/[I] = 107M-1S-1). Reacts with thrombin in a 1:1 stoichiometry. Can also be used to inhibit tissue plasminogen activator, urokinase, Factor VIIa, and Factor XIa.

Mouse PAI-1 (wild type active fraction)

(MPAI)

Recombinant mouse PAI-1 is the ideal choice for studies involving this animal model of fibrinolysis and cancer studies. The active fraction typically contains 30-70percent active PAI-1.

Mouse PAI-1 (stable mutant)

(MPAI-I91L)

Using information obtained from the studies which produced stabilized human PAI-1 (CPAI) we have available a form of mouse PAI-1 that has a single conservative mutation (Isoleucine 91 to Leucine).

Mouse PAI-1 genetically deficient brain

(MPAI-KO-BR)

This organ is an ideal negative control for western blotting, immunoprecipitation, immunohistochemistry and other experiments involving PAI-1. Collected and flash frozen from homozygous PAI-1 knockout mice of strain B6.129S2-Serpine1 tm1Mlg /J. The targeted PAI-1 knockout mutation was made on the C57BL/6J background strain. Mice homozygous for this mutation develop normally and are viable and fertile. Compared to wild type mice, pulmonary clot lysis is increased in the heterozygote and further increased in the homozygote. Endotoxin induced venous thrombosis is decreased compared to wild type mice. Thus, disruption of the Serpine1 gene induces a mild hyperfibrinolytic state. Hemostasis is normal in homozygous mutants.

References:
Carmeliet P; Kieckens L; Schoonjans L; Ream B; van Nuffelen A; Prendergast G; Cole M; Bronson R; Collen D; Mulligan RC. 1993. Plasminogen activator inhibitor-1 gene-deficient mice. I. Generation by homologous recombination and characterization. J Clin Invest 92(6):2746-55.

Mouse PAI-1 genetically deficient heart

(MPAI-KO-HT)

This organ is an ideal negative control for western blotting, immunoprecipitation, immunohistochemistry and other experiments involving PAI-1. Collected and flash frozen from homozygous PAI-1 knockout mice of strain B6.129S2-Serpine1 tm1Mlg /J. The targeted PAI-1 knockout mutation was made on the C57BL/6J background strain. Mice homozygous for this mutation develop normally and are viable and fertile. Compared to wild type mice, pulmonary clot lysis is increased in the heterozygote and further increased in the homozygote. Endotoxin induced venous thrombosis is decreased compared to wild type mice. Thus, disruption of the Serpine1 gene induces a mild hyperfibrinolytic state. Hemostasis is normal in homozygous mutants.

References:
Carmeliet P; Kieckens L; Schoonjans L; Ream B; van Nuffelen A; Prendergast G; Cole M; Bronson R; Collen D; Mulligan RC. 1993. Plasminogen activator inhibitor-1 gene-deficient mice. I. Generation by homologous recombination and characterization. J Clin Invest 92(6):2746-55.

Mouse PAI-1 genetically deficient kidney

(MPAI-KO-KD)

This organ is an ideal negative control for western blotting, immunoprecipitation, immunohistochemistry and other experiments involving PAI-1. Collected and flash frozen from homozygous PAI-1 knockout mice of strain B6.129S2-Serpine1 tm1Mlg /J. The targeted PAI-1 knockout mutation was made on the C57BL/6J background strain. Mice homozygous for this mutation develop normally and are viable and fertile. Compared to wild type mice, pulmonary clot lysis is increased in the heterozygote and further increased in the homozygote. Endotoxin induced venous thrombosis is decreased compared to wild type mice. Thus, disruption of the Serpine1 gene induces a mild hyperfibrinolytic state. Hemostasis is normal in homozygous mutants.

References:
Carmeliet P; Kieckens L; Schoonjans L; Ream B; van Nuffelen A; Prendergast G; Cole M; Bronson R; Collen D; Mulligan RC. 1993. Plasminogen activator inhibitor-1 gene-deficient mice. I. Generation by homologous recombination and characterization. J Clin Invest 92(6):2746-55.

Mouse PAI-1 genetically deficient lung

(MPAI-KO-LG)

This organ is an ideal negative control for western blotting, immunoprecipitation, immunohistochemistry and other experiments involving PAI-1. Collected and flash frozen from homozygous PAI-1 knockout mice of strain B6.129S2-Serpine1 tm1Mlg /J. Each mouse lung consists of 5 lobes. The targeted PAI-1 knockout mutation was made on the C57BL/6J background strain. Mice homozygous for this mutation develop normally and are viable and fertile. Compared to wild type mice, pulmonary clot lysis is increased in the heterozygote and further increased in the homozygote. Endotoxin induced venous thrombosis is decreased compared to wild type mice. Thus, disruption of the Serpine1 gene induces a mild hyperfibrinolytic state. Hemostasis is normal in homozygous mutants.

References:
Carmeliet P; Kieckens L; Schoonjans L; Ream B; van Nuffelen A; Prendergast G; Cole M; Bronson R; Collen D; Mulligan RC. 1993. Plasminogen activator inhibitor-1 gene-deficient mice. I. Generation by homologous recombination and characterization. J Clin Invest 92(6):2746-55.

Mouse PAI-1 genetically deficient liver

(MPAI-KO-LR)

This organ is an ideal negative control for western blotting, immunoprecipitation, immunohistochemistry and other experiments involving PAI-1. Collected and flash frozen from homozygous PAI-1 knockout mice of strain B6.129S2-Serpine1 tm1Mlg /J. The targeted PAI-1 knockout mutation was made on the C57BL/6J background strain. Mice homozygous for this mutation develop normally and are viable and fertile. Compared to wild type mice, pulmonary clot lysis is increased in the heterozygote and further increased in the homozygote. Endotoxin induced venous thrombosis is decreased compared to wild type mice. Thus, disruption of the Serpine1 gene induces a mild hyperfibrinolytic state. Hemostasis is normal in homozygous mutants.

References:
Carmeliet P; Kieckens L; Schoonjans L; Ream B; van Nuffelen A; Prendergast G; Cole M; Bronson R; Collen D; Mulligan RC. 1993. Plasminogen activator inhibitor-1 gene-deficient mice. I. Generation by homologous recombination and characterization. J Clin Invest 92(6):2746-55.

Mouse PAI-1 genetically deficient plasma, sodium citrate

(MPAI-KO-SC)

This plasma is an ideal negative control for ELISA based assays and other experiments involving PAI-1. Collected from homozygous PAI-1 knockout mice of strain B6.129S2-Serpine1 tm1Mlg /J. Anticoagulated with sodium citrate and flash frozen. Collected from male mice. Female mouse plasma is also available.
The targeted PAI-1 knockout mutation was made on the C57BL/6J background strain. Mice homozygous for this mutation develop normally and are viable and fertile. Compared to wild type mice, pulmonary clot lysis is increased in the heterozygote and further increased in the homozygote. Endotoxin induced venous thrombosis is decreased compared to wild type mice. Thus, disruption of the Serpine1 gene induces a mild hyperfibrinolytic state. Hemostasis is normal in homozygous mutants.

References:
Carmeliet P; Kieckens L; Schoonjans L; Ream B; van Nuffelen A; Prendergast G; Cole M; Bronson R; Collen D; Mulligan RC. 1993. Plasminogen activator inhibitor-1 gene-deficient mice. I. Generation by homologous recombination and characterization. J Clin Invest 92(6):2746-55.

Mouse PAI-1 genetically deficient spleen

(MPAI-KO-SP)

This organ is an ideal negative control for western blotting, immunoprecipitation, immunohistochemistry and other experiments involving PAI-1. Collected and flash frozen from homozygous PAI-1 knockout mice of strain B6.129S2-Serpine1 tm1Mlg /J. The targeted PAI-1 knockout mutation was made on the C57BL/6J background strain. Mice homozygous for this mutation develop normally and are viable and fertile. Compared to wild type mice, pulmonary clot lysis is increased in the heterozygote and further increased in the homozygote. Endotoxin induced venous thrombosis is decreased compared to wild type mice. Thus, disruption of the Serpine1 gene induces a mild hyperfibrinolytic state. Hemostasis is normal in homozygous mutants.

References:
Carmeliet P; Kieckens L; Schoonjans L; Ream B; van Nuffelen A; Prendergast G; Cole M; Bronson R; Collen D; Mulligan RC. 1993. Plasminogen activator inhibitor-1 gene-deficient mice. I. Generation by homologous recombination and characterization. J Clin Invest 92(6):2746-55.

Active mouse PAI-1 functional assay ELISA kit

(MPAIKT)

Plasminogen activator inhibitor type 1 (PAI-1) is involved in the regulation of the blood fibrinolytic system. Increased plasma levels of PAI-1 are implicated in the impairment of fibrinolytic function and may be associated with thrombotic diseases. Levels of PAI-1 increase with age and are elevated in conditions such as normal pregnancy and sepsis. The sensitive quantitative measurement of functionally active mouse PAI-1 in plasma samples is easily performed with this 96 well strip format ELISA kit. The concentration level of PAI-1 antigen in mouse plasma was found to be 1.9 ng/ml. The assay measures active PAI-1 in the 0.05-50 ng/ml range. Samples giving mouse PAI-1 levels above 50 ng/ml should be diluted in blocking buffer before use. Normal plasma should be applied directly to the plate for best results. It is important to ensure a platelet free preparation of plasma as platelets can release PAI-1. Functionally active PAI-1 reacts with urokinase coated onto a micro titer plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected by the assay. Cross-reactivity: 29percent rat PAI-1, 18percent rabbit PAI-1, 0.6percent porcine PAI-1, 76percent human PAI-1. After appropriate washing steps, anti-mouse PAI-1 primary antibody binds to the captured enzyme. Excess antibody is washed away, and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of PAI-1 in the samples. A standard calibration curve is prepared using dilutions of purified PAI-1 and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Negative control: PAI-1 genetically deficient mouse plasma
Suggested additional reagents: 10X Wash Buffer, TMB Substrate, uPA Plate, Secondary Antibody

Mouse PAI-1 total antigen assay ELISA kit

(MPAIKT-TOT)

Plasminogen activator inhibitor type 1 (PAI-1) is involved in the regulation of the blood fibrinolytic system. Increased plasma levels of PAI-1 are implicated in the impairment of fibrinolytic function and may be associated with thrombotic diseases. Levels of PAI-1 increase with age and are elevated in conditions such as normal pregnancy and sepsis. The sensitive quantitative measurement of total mouse PAI-1 antigen in plasma samples is easily performed with this 96 well strip format ELISA kit. The concentration level of PAI-1 antigen in mouse plasma was found to be 1.9 ng/ml. The assay measures mouse PAI-1 in the 0.05-50 ng/ml range. Samples giving mouse PAI-1 levels above 50 ng/ml should be diluted in blocking buffer before use. Normal plasma should be applied directly to the plate for best results. It is important to ensure a platelet free preparation of plasma as platelets can release PAI-1. Mouse PAI-1 will bind to the capture antibody coated on the microtiter plate. Free, latent, and complexed PAI-1 will be detected by the assay. Cross-reactivity: 2percent porcine PAI-1, 1.7percent human PAI-1, 0.5percent rabbit PAI-1, 0.3percent rat PAI-1. After appropriate washing steps, anti-mouse PAI-1 primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of mouse PAI-1 in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified mouse PAI-1 and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Negative control: PAI-1 genetically deficient mouse plasma
Suggested additional reagents: 10X Wash Buffer, TMB Substrate, Mouse PAI-1 Antigen Capture Plate, Secondary Antibody

Mouse PAI-1 (wild type latent form)

(MPAI-L)

Latent mouse PAI-1 is a useful control for experiments when used in conjunction with the active form.

Mouse PAI-1 (Biotin labeled latent fraction)

(MPAI-L-BIO)

This latent fraction of recombinant mouse PAI-1 is biotin labeled on lysine residues. Biotin labeled latent mouse PAI-1 is a useful control for experiments involving active proteins.

Mouse PAI-1 Antigen Capture Plate

(MPAI-TOT-PLATE)

96 well plate with 8 removable strips coated with monoclonal antibody to mouse Plasminogen Activator Inhibitor (PAI-1) at 100 ul/well and blocked with 300 ul/well. Ideal for specific binding of mouse PAI-1 antigen for sandwich style ELISA experiments.

Mouse PAI-1 tPA complex

(MPAI-TPA)

Mouse sc-tPA is reacted with an excess of mouse PAI-1 stable mutant at physiological pH. The resulting 1:1 molar complex is purified from the remaining PAI-1 by affinity chromatography with immobilized antibodies to mouse tPA.

Mouse pancreatic elastase

(MPE)

Chromatographically prepared from the euglobin fraction of pancreas by the method of Lewis et al. (1) then twice crystallized. The protein is stable 9-12 months when stored at 5 C and does not contain trypsin or chymotrypsin. 95percent pure by SDS-PAGE. Reconstitute with 0.05M Na Acetate 0.1M NaCl pH 5.0 to 1 mg/ml or desired concentration, aliquot and freeze remaining portion. Assay: 5-10 ug in 0.1M Tris pH 8.8 at 37 C containing 0.2mM Suc-(Al)3-pNA.
References:
1. Lewis, U.J. et al. (1956) J. Biol. Chem. 22:705

Mouse Platelet Factor 4, Recombinant

(MPF4-R)

Mouse PF-4 is expressed recombinantly and purified by chromatography. The purified protein in PBS is sterile filtered and lyophilized. Protein identity is confirmed by mass spectrometry (8.2 kDa monomer) and N-terminal sequencing (M-G-P-E-E). Purity is >95 percent by SDS-PAGE. Add deionized water to original volume, aliquot and freeze unused portion.

Mouse PAI-1 depleted plasma, sodium citrate

(MPLA-SC-PAI)

Prepared from frozen mouse plasma using immobilized anti-mouse PAI-1 IgG.

Plasminogen Depleted Mouse Serum

(MPLA-SER-PG)

Prepared from frozen mouse serum.

Mouse plasminogen

(MPLG)

Prepared from fresh mouse plasma by immobilized lysine chromatography.

Mouse plasminogen total antigen assay ELISA kit

(MPLGKT-TOT)

Plasminogen is a single chain glycoprotein zymogen and is the precursor of the fibrinolytic enzyme plasmin. Plasminogen deficiencies are classified as hypoplasminogenemia (Type I) or dysplasminogenemia (Type 2) and are associated with decreased extracellular fibrin clearance leading to mucous membrane lesions and ligneous conjunctivitis. The sensitive quantitative measurement of total mouse plasminogen antigen in plasma, serum, or cell culture media samples is easily performed with this 96 well strip format ELISA kit. Plasminogen, plasmin and plasmin antiplasmin complex will be detected by the assay. Plasminogen antigen was found to be 84 ug/ml in a small sample of normal mice. The assay measures total mouse plasminogen in the 2.5-500 ng/ml range. Samples giving mouse plasminogen levels above 500 ng/ml should be diluted in a similar fluid depleted of plasminogen or blocking buffer before use. A 1:1,000-1:10,000 dilution for plasma is suggested for best results. Mouse plasminogen will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, anti-mouse plasminogen primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of plasminogen in the samples. A standard calibration curve is prepared using dilutions of purified plasminogen and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Mouse plasmin

(MPLM)

Prepared from plasminogen by activation with immobilized human uPA. 100 percent functionally active plasmin is purified from the activation reaction by immobilized SBTI. Plasmin will undergo rapid auto proteolysis in the absence of benzamidine and should be used quickly once thawed. Aliquot to avoid freeze-thaw cycles.

Mouse Protease Nexin 1, native recombinant

(MPN1)

Mouse Protease Nexin 1 (PN-1), recombinantly produced in insect cells. Active and fully complexable with tissue-type plasminogen activator. This SERPIN inhibits serine proteases such as thrombin, tPA and uPA, and is evolutionarily related to antithrombin and heparin cofactor II.

Myeloperoxidase

(MPO)

Myeloperoxidase purified from leucocytes of purulent human sputum which is >98percent pure. It is a lysosomal protein stored in azurophilic granules of the neutrophil which produces hypochlorous acid from hydrogen peroxide and chloride anion during the neutrophil respiratory burst. Supplied as a lyophilized, salt-free, green soluble powder which is stable for over a year when stored at 5 C. 150-160 units per mg of protein. 1 unit will decompose 1 umole of H2O2 per minute at pH 7.0 and 25 C using 4-amino-antipyrine as hydrogen donor. Recommended assay buffer: 0.1 M KPO4; pH 7.0

Mouse Prorenin, C-terminal 8x His tag

(MPREN-HIS)

Recombinantly produced in HEK cell culture and purified by chelated metal affinity chromatography. Contains a 8X-Histidine tag at C terminus for purification. Resistant to activation to renin by trypsin digestion. Prorenin is a glycosylated aspartic protease that consists of 2 homologous lobes and is the precursor of renin. Prorenin exhibits a low level of enzymatic activity relative to renin which is generated from prorenin by proteolytic cleavage of the first ~43 amino acids at the N-terminus. This so called prosegment appears to block the full enzymatic potential of the active site (1). Renin activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE, the angiotensin-converting enzyme primarily within the capillaries of the lungs. It has been reported that the levels of circulating prorenin (but not renin) are increased in diabetic subjects(2).

1) A.H. Jan Danser; Jaap Deinum ; Renin, Prorenin and the Putative (Pro)renin Receptor). Hypertension. 2005;46:1069.

2) Luetscher JA, Kraemer FB, Wilson DM, Schwartz HC, Bryer-Ash M. Increased plasma inactive renin in diabetes mellitus. A marker of microvascular complications. N Engl J Med. 1985;312:1412-1417.

Balb C Mouse IgG, Protein A Purified

(MSBC-GF)

Purified from normal serum by immobilized Protein A. >95 percent pure by SDS-PAGE and preservative free.

C57BL6 Mouse IgG, Protein A Purified

(MSC57BL6-GF)

Purified from normal serum by immobilized Protein A. >95 percent pure by SDS-PAGE and preservative free.

CD1 Mouse IgG, Protein A Purified

(MSCD1-GF)

Purified from normal serum by immobilized Protein A. >95 percent pure by SDS-PAGE and preservative free.

Mouse IgG, Protein A Purified

(MS-GF)

Purified from normal serum by immobilized Protein A. >95 percent pure by SDS-PAGE and preservative free.

Mouse IgG, Protein A Purified, Low Endotoxin

(MS-GF-ED)

Purified from normal serum by immobilized Protein A using low endotoxin methodology. The purified protein is sterile filtered and lyophilized from azide fee PBS. >95percent pure by SDS-PAGE and

Mouse Thrombin

(MTHROM)

Mouse thrombin is prepared from purified mouse prothrombin by activation with Saw Scaled Viper Venom. The venom was removed after activation. Complete activation is observed on SDS-PAGE.

Active mouse tPA, recombinant

(MTPA)

Recombinantly produced in insect cells. >95percent active.

Non-enzymatic & non-cleavable Mouse tPA

(MTPA-ALANC)

Mouse tPA with a double mutation that is permanently single chain and lacks catalytic activity. The non-catalytic mutation site is S481A on the mature protein and S510A on the complete mRNA sequence (UniProtKB: locus TPA_MOUSE, accession P11214). The non-cleavable mutation site is 279E on the mature protein and R308E on the complete mRNA sequence (UniProtKB: locus TPA_MOUSE, accession P11214). Recombinantly produced in insect cells.

Biotin labeled Mouse tPA

(MTPA-BIO)

Recombinantly produced in insect cells and biotin labeled.

Fluorescein labeled Mouse tPA

(MTPA-FITC)

Recombinantly produced in insect cells and fluorescein labeled.

Active mouse tPA functional assay ELISA kit

(MTPAKT)

Tissue plasminogen activator (tPA) is a serine protease that converts plasminogen to plasmin in the blood fibrinolytic system. It also plays an important role in the nervous system, including the processes of neuronal migration, neurite outgrowth, and neuronal plasticity. tPA has been suggested to have a role in several neuropathological conditions such as cerebral ischemia, seizures, and demyelinating diseases. The sensitive quantitative measurement of functionally active mouse tPA in plasma and other biological fluid samples is easily performed with this 96 well strip format ELISA kit. The level of active tPA in normal plasma was found to be 9.9 ng/ml in NSA/CF-1, 1.4 ng/ml in C57BL6, and 0.4 ng/ml in CD-1 mouse strains. The assay measures active tPA in the 0.05-50 ng/ml range. Samples giving mouse tPA levels above 50 ng/ml should be diluted in blocking buffer before use. Samples of plasma in citrate or EDTA may be assayed with this kit. Plasma in heparin is not recommended. It is important to ensure a platelet free preparation of plasma as platelets can release PAI-1, which in turn could potentially form a complex with active tPA. If plasma samples were collected in citrate the pH should be brought up to neutral with the 10X TBS provided in the kit. Serum and cell culture media at neutral pH may also be used. Functionally active tPA will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. Complexed tPA will not bind to the PAI-1 and will not be detected by the assay. After appropriate washing steps, anti-mouse tPA primary antibody binds to the captured enzyme. Excess antibody is washed away, and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of active tPA in the samples. A standard calibration curve is prepared using dilutions of purified tPA and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

This assay uses an exclusive enzyme capture technology to only detect functionally active protein.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, Avidin Plate, Secondary Antibody

Mouse tPA total antigen assay ELISA kit

(MTPAKT-TOT)

Tissue plasminogen activator (tPA) is a serine protease that converts plasminogen to plasmin in the blood fibrinolytic system. It also plays an important role in the nervous system, including the processes of neuronal migration, neurite outgrowth, and neuronal plasticity. tPA has been suggested to have a role in several neuropathological conditions such as cerebral ischemia, seizures, and demyelinating diseases. The sensitive quantitative measurement of total mouse tPA antigen in plasma and other biological fluid samples is easily performed with this 96 well strip format ELISA kit. The level of tPA antigen in normal plasma was found to be 9.4 ng/ml in NSA/CF-1, 2.4 ng/ml in C57BL6, and 0.4 ng/ml in CD-1 mouse strains. The assay measures total tPA in the 0.1-50 ng/ml range. Samples giving mouse tPA levels above 50 ng/ml should be diluted in blocking buffer before use. Mouse tPA will bind to the capture antibody coated on the microtiter plate. Free and complexed tPA will be detected by the assay. After appropriate washing steps, anti-mouse tPA primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of mouse tPA in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified mouse tPA and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
Suggested additional reagents: 10X Wash Buffer, TMB Substrate, tPA Antigen Capture Plate, Secondary Antibody

Non-cleavable Mouse tPA

(MTPA-NC)

The addition of an arginine to glutamic acid mutation generates mouse tPA that is 100percent single chain and resistant to cleavage by plasmin. The mutation site is R279E on the mature protein and R308E on the complete mRNA sequence (UniProtKB: locus TPA_MOUSE, accession P11214). Recombinantly produced in insect cells.

Non-enzymatic Mouse tPA

(MTPA-S481A)

This single chain mouse tPA has the active site serine mutated to alanine rendering the enzyme catalytically inactive. The mutation site is S481A on the mature protein and S510A on the complete mRNA sequence (UniProtKB: locus TPA_MOUSE, accession P11214). The tPA still retains exosite binding as well as other biological properties of tPA including but not limited to surface binding to fibrin. This protein is not sterile and should be passed through a 0.22 micron filter before use in cell culture.

Active mouse tPA, two chain recombinant

(MTPA-TC)

Recombinantly produced in insect cells. Activated from single-chain form with immobilized plasmin. >95percent two chain, >95percent active.

Mouse tPA Antigen Capture Plate

(MTPA-TOT-PLATE)

96 well plate with 8 removable strips coated with monoclonal antibody to mouse Tissue-type Plasminogen Activator (tPA) at 100 ul/well and blocked with 300 ul/well. Ideal for specific binding of mouse tPA antigen for sandwich style ELISA experiments. Cross reacts with rat tPA.

Active Mouse Urokinase, HMW

(MUPA)

Active two-chain HMW mouse urokinase, recombinantly produced in insect cells.

Active Mouse Urokinase, Alexa Fluor 700 Labeled

(MUPA-AF700)

Active two-chain HMW mouse urokinase, recombinantly produced in insect cells. Labeled with Alexa Fluor 700 at primary amines (Abs: 702, Em: 723).

Mouse HMW tc-uPA, biotin conjugate

(MUPA-BIO)

Biotin labeled Mouse Urokinase.

Mouse HMW tc-uPA, fluorescein conjugate

(MUPA-FITC)

Fluorescein labeled Mouse Urokinase.

Active mouse uPA functional assay ELISA kit

(MUPAKT)

Urokinase plasminogen activator (uPA) is a serine protease that activates plasminogen to plasmin in the blood fibrinolytic system. It is also implicated in events related to cell invasion/migration. The sensitive quantitative measurement of functionally active mouse uPA in plasma and other biological fluid samples is easily performed with this 96 well strip format ELISA kit. The concentration level of uPA antigen in mouse urine has been reported to be 1.8 ug/ml. The assay measures active uPA in the 0.025-10 ng/ml range. Samples giving mouse uPA levels above 10 ng/ml should be diluted in blocking buffer before use. It is important to ensure a platelet free preparation of plasma as platelets can release PAI-1, which in turn could potentially form a complex with active uPA. If plasma samples were collected in citrate the pH should be brought up to neutral with the 10X TBS provided in the kit. Serum and cell culture media at neutral pH may also be used. If using kidney extracts that have been extracted using triton X, dialyze to remove the triton X before using in the assay. Detergents such as triton X may interfere with the assay. Functionally active uPA will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. Inactive or complexed uPA will not bind to the PAI-1 and will not be detected by the assay. Cross-reactivity: 1percent Human uPA. After appropriate washing steps, anti-mouse uPA primary antibody binds to the captured enzyme. Excess antibody is washed away, and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of active uPA in the samples. A standard calibration curve is prepared using dilutions of purified uPA and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

This assay uses an exclusive enzyme capture technology to only detect functionally active protein.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, Avidin Plate, Secondary Antibody

Mouse uPA total antigen assay ELISA kit

(MUPAKT-TOT)

Urokinase plasminogen activator (uPA) is a serine protease that activates plasminogen to plasmin in the blood fibrinolytic system. It is also implicated in events related to cell invasion/migration. The sensitive quantitative measurement of total mouse uPA antigen in plasma and other biological fluid samples is easily performed with this 96 well strip format ELISA kit. The concentration level of uPA antigen in mouse urine has been reported to be 1.8 ug/ml. The assay measures total uPA in the 0.01-10 ng/ml range. Samples giving mouse uPA levels above 10 ng/ml should be diluted in blocking buffer before use. Mouse uPA will bind to the capture antibody coated on the microtiter plate. Free and complexed uPA will be detected by the assay. This kit does not cross react with human uPA. After appropriate washing steps, anti-mouse uPA primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of mouse uPA in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified mouse uPA and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, uPA Antigen Capture Plate, Secondary Antibody

Active Mouse Urokinase, LMW

(MUPA-LMW)

Low molecular weight active mouse urokinase, recombinantly produced in insect cells.

Mouse multimeric vitronectin

(MVN)

Prepared from fresh mouse plasma using urea as a denaturant.

Mouse vitronectin total antigen assay ELISA kit

(MVNKT-TOT)

Vitronectin is an abundant plasma glycoprotein that helps regulate coagulation, fibrinolysis, complement activation, and cell adhesion. Vitronectin binds to glycosaminoglycans, collagen, plasminogen and urokinase receptors. It also may control the clearance of vascular thrombi by binding and stablilizing PAI-1. In binding PAI-1, it extends the lifetime of active PAI-1. Vitronectin may also be involved in the regulation of bone metabolism. The sensitive quantitative measurement of total mouse vitronectin antigen in plasma, serum, culture media or tissue extract samples is easily performed with this 96 well strip format ELISA kit. The vitronectin concentration in mouse serum has been estimated at 300 ug/ml by semi-quantitative immunoblotting. The assay measures total vitronectin in the 5-1000 ng/ml range. Samples giving mouse vitronectin levels above 1000 ng/ml should be diluted in blocking buffer before use. A 1:100 to 1:1,000 dilution for mouse plasma or serum is suggested for best results. Mouse vitronectin will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-mouse vitronectin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with avidin conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of mouse vitronectin in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified mouse vitronectin and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
Suggested additional reagents: 10X Wash Buffer, TMB Substrate, Mouse Vitronectin Antigen Capture Plate, Avidin-HRP

Mouse Vitronectin Antigen Capture Plate

(MVN-TOT-PLATE)

96 well plate with 8 removable strips coated with affinity purified polyclonal antibody to mouse Vitronectin at 100 ul/well and blocked with 300 ul/well. Ideal for specific binding of mouse vitronectin antigen for sandwich style ELISA experiments.

Mouse Protein Z-Dependent Protease Inhibitor, native recombinant

(MZPI)

Mouse Protein Z-Dependent Protease Inhibitor (ZPI), recombinantly produced in insect cells. Active and complexable with Factor XIa. This Serine Protease Inhibitor (SERPIN) requires the cofactors Protein Z, phospholipids and calcium to inhibit endogenous Factor Xa. ZPI contains a tyrosine residue at the P1 position of the reactive center loop, rather than arginine which is present in most SERPINs.
References
1. Han, X. et al. (1998) PNAS 95:9250?9255.
2. Huang, X. et al. (2008) J. Biol. Chem. 283:29770?29783.

Human PAI-1 (N-terminal Alexa Fluor 488 labeled stable mutant)

(NTAF488CPAI)

Stable mutant human PAI-1 labeled with Alexa Fluor 488 by iodoacetamide substitution at the N-terminal cysteine (patent pending).

Human PAI-1 (N-terminal Alexa Fluor 488 labeled, active fraction)

(NTAF488PAI-A)

Active human PAI-1 labeled with Alexa Fluor 488 by iodoacetamide substitution at the N-terminal cysteine (patent pending).

Human PAI-1 (N-terminal Alexa Fluor 488 labeled, latent fraction)

(NTAF488PAI-L)

Latent human PAI-1 labeled with Alexa Fluor 488 by iodoacetamide substitution at the N-terminal cysteine (patent pending).

Human PAI-1 (N-terminal Alexa Fluor 594 labeled stable mutant)

(NTAF594CPAI)

Stable mutant human PAI-1 labeled with Alexa Fluor 594 by iodoacetamide substitution at the N-terminal cysteine (patent pending).

Human PAI-1 (N-terminal Alexa Fluor 594 labeled, active fraction)

(NTAF594PAI-A)

Active human PAI-1 labeled with Alexa Fluor 594 by iodoacetamide substitution at the N-terminal cysteine (patent pending).

Human PAI-1 (N-terminal Alexa Fluor 594 labeled, latent fraction)

(NTAF594PAI-L)

Latent human PAI-1 labeled with Alexa Fluor 594 by iodoacetamide substitution at the N-terminal cysteine (patent pending).

Human PAI-1 (N-terminal biotin labeled stable mutant)

(NTBIOCPAI)

Stable mutant human PAI-1 biotin labeled by iodoacetamide substitution at the N-terminal cysteine (patent pending).

Human PAI-1 (N-terminal biotin labeled, active fraction)

(NTBIOPAI-A)

Active human PAI-1 biotin labeled by iodoacetamide substitution at the N-terminal cysteine (patent pending).

Human PAI-1 (N-terminal biotin labeled, latent fraction)

(NTBIOPAI-L)

Latent human PAI-1 biotin labeled by iodoacetamide substitution at the N-terminal cysteine (patent pending).

Human PAI-1 (N-terminal cysteine stable mutant)

(NTCYSCPAI)

This stable form of PAI-1 contains an N-terminal cysteine mutation in addition to the four stabilization mutations (patent pending). The protein is supplied in buffer with 1mM DTT to prevent dimerization. To label with thiol reactive dye, buffer exchange into degassed 0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; pH 6.6 by desalting column. Collect into 10 fold molar excess of maleimide or iodoacetamide dye and incubate for 1 hour at room temperature. To preserve PAI-1 activity minimize the percentage of DMSO/DMF in the reaction. Remove free dye by desalting, ammonium sulfate precipitation, or dialysis.

Human PAI-1 (N-terminal cysteine, active fraction)

(NTCYSPAI-A)

Wild type PAI-1 cloned with a cysteine residue at the N-terminus (patent pending). The active fraction is >95percent pure and >95percent active as determined by titration with HWM tc-urokinase and SDS PAGE. The protein is supplied in buffer with 1 mM DTT to prevent dimerization. To label with thiol reactive dye, buffer exchange into degassed 0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; pH 6.6 by desalting column. Collect into 10 fold molar excess of maleimide or iodoacetamide dye and incubate for 1 hour at room temperature. To preserve PAI-1 activity minimize the percentage of DMSO/DMF in the reaction. Remove free dye by desalting, ammonium sulfate precipitation, or dialysis.

Human PAI-1 (N-terminal cysteine mutant, latent fraction)

(NTCYSPAI-L)

Wild type PAI-1 cloned with a cysteine residue at the N-terminus (patent pending). The latent fraction is

Human PAI-1 (N-terminal fluorescein labeled stable mutant)

(NTFLCPAI)

Stable mutant human PAI-1 fluorescein labeled by iodoacetamide substitution at the N-terminal cysteine (patent pending).

Human PAI-1 (N-terminal fluorescein labeled, active fraction)

(NTFLPAI-A)

Active human PAI-1 fluorescein labeled by iodoacetamide substitution at the N-terminal cysteine (patent pending).

Human PAI-1 (N-terminal fluorescein labeled, latent fraction)

(NTFLPAI-L)

Latent human PAI-1 fluorescein labeled by iodoacetamide substitution at the N-terminal cysteine (patent pending).

Mouse monoclonal to human PAI-1

(PAI3A120)

Non-inhibitory monoclonal antibody to human PAI-1. Strongly reactive with human PAI-1 free and complexed with tPA. IgG fraction purified by immobilized Protein A. Isotype IgG.

Mouse monoclonal to human PAI-1

(PAI3C311)

Non-inhibitory monoclonal antibody to human PAI-1. Produces excellent western blots with both free and complexed forms of PAI-1. Strongly reactive with human, rat, rabbit, and porcine PAI-1. IgG fraction purified by immobilized Protein A. Isotype IgG.

Mouse monoclonal to human PAI-1

(PAI3E529)

Non-inhibitory monoclonal antibody to human PAI-1. Recommended for detection of human PAI-1 in ELISA. Purified by immobilized Protein G. IgG1k class.

Human PAI-1 (wild type active fraction)

(PAI-A)

Human wild type PAI-1 is produced as an active and latent form in E. Coli. The purification conditions are gentle and result in an active fraction >99percent pure and >98percent active as determined by titration with HMW tc-urokinase and SDS PAGE.

Peptide annealed human PAI-1

(PAI-A-AN)

Active human wild-type (wt) PAI-1 is also available pre-annealed with the Octapeptide Ac-TVASSSTA and ready for immediate use. Please inquire regarding other species of annealed PAI-1.

Human PAI-1 (wild type – elastase cleaved)

(PAI-C)

Human PAI-1 is provided cleaved at P3-P4 residues via immobilized elastase:

Human PAI-1 (wild type latent fraction)

(PAI-L)

Ac-TVASSSTA Octapeptide

(PEP-1)

Ac-TVASSSTA is an Octapeptide mimic of the N-terminal residues of the reactive center loop of PAI-1 (cf. P14-P7 residues). The Octapeptide inactivates PAI-1 by substituting for strand 4 in beta-sheet A in a manner that competes with formation of the latent species. Insertion of the peptide into beta-sheet A effectively forms a stable complex that converts PAI-1 into a substrate for tissue-type plasminogen activator (tPA) and other target proteinases. The resulting species of PAI-1 is thermodynamically stable and is useful for investigating the role of reactive center loop insertion. Supplied as a lyophilized powder. Add deionized water to original volume, aliquot and freeze unused portion.

Porcine Fibrinogen

(PFBGN)

Prepared from fresh porcine plasma using several chromatographic steps. Plasminogen depleted by lysine affinity chromatography. Thaw protein in a 37C water bath. Aliquot and freeze the unused portion. Keep fibrinogen at 25-37C, aliquot and flash freeze unused portion.

FITC Labeled Porcine Fibrinogen

(PFBGN-FITC)

Porcine fibrinogen amino terminal labeled with fluorescein isothiocyanate (FITC). Prepared from fresh porcine plasma using several chromatographic steps. Plasminogen depleted by lysine affinity chromatography. Thaw at 37C without agitation until completely liquid, then gently mix before use. Keep fibrinogen at 25-37C, aliquot and flash freeze unused portion.

Anti Human Prekallikrein, Clone 20E7

(PK20E7)

Mouse monoclonal antibody to human prekallikrein. Strongly blots prekallikrein and kallikrein under non-reducing and reducing conditions. Binding epitope is on the light chain of kallikrein. IgG fraction purified by immobilized Protein G. Clone 20E7-B7, isotype IgG2b.

Anti Human Plasminogen, Clone 11B5-B2

(PLG11B5B2)

Mouse monoclonal antibody to human plasminogen. IgG fraction purified by immobilized Protein G. Detects plasminogen and plasmin under non-reducing and reducing conditions. Epitope localized to the heavy chain. Clone 11B5-B2, isotype IgG1kappa.

Click here to compare all our monoclonal antibodies to human plasminogen

Anti Human Plasminogen, Clone 13C3-B10

(PLG13C3B10)

Mouse monoclonal antibody to human plasminogen. IgG fraction purified by immobilized Protein G. Detects plasminogen and plasmin under non-reducing and reducing conditions. Epitope localized to the light chain. Clone 13C3-B10, isotype IgG1kappa.

Click here to compare all our monoclonal antibodies to human plasminogen

Anti Human Plasminogen, Clone 1C10-F2

(PLG1C10F2)

Mouse monoclonal antibody to human plasminogen. IgG fraction purified by immobilized Protein G. Detects plasminogen and plasmin under non-reducing and reducing conditions. Epitope localized to the light chain. Clone 1C10-F2, isotype IgG1kappa.

Click here to compare all our monoclonal antibodies to human plasminogen

Anti Human Plasminogen, Clone 2F6-C6

(PLG2F6C6)

Mouse monoclonal antibody to human plasminogen. IgG fraction purified by immobilized Protein A. Detects plasminogen and plasmin under non-reducing and reducing conditions. Epitope localized to the light chain. Clone 2F6-C6, isotype IgG2bkappa.

Click here to compare all our monoclonal antibodies to human plasminogen

Anti Human Plasminogen, Clone 4G6-B11

(PLG4G6B11)

Mouse monoclonal antibody to human plasminogen. IgG fraction purified by immobilized Protein G. Detects plasminogen and plasmin under non-reducing and reducing conditions. Epitope localized to the light chain. Clone 4G6-B11, isotype IgG1kappa.

Click here to compare all our monoclonal antibodies to human plasminogen

Anti Human Plasminogen, Clone 9F9-C4

(PLG9F9C4)

Mouse monoclonal antibody to human plasminogen. IgG fraction purified by immobilized Protein G. Detects plasminogen and plasmin under non-reducing conditions. Epitope localized to Kringle 1 on the heavy chain. Clone 9F9-C4, isotype IgG1kappa.

Click here to compare all our monoclonal antibodies to human plasminogen

Anti Human Plasminogen MAb Sample Pack

(PLG-MABPACK)

100 ug of each of PLG949C4, PLG11B5B2, PLG2F6C6, PLG1C10F2, PLG13C3B10, and PLG4G6B11.

Porcine PAI-1 (wild type active form)

(POPAI)

Porcine (pig) PAI-1 displays a close homology to bovine and human PAI-1 with 90 and 86percent amino acid homology, respectively.

Active porcine PAI-1 functional assay ELISA kit

(POPAIKT)

Plasminogen activator inhibitor type 1 (PAI-1) is involved in the regulation of the blood fibrinolytic system. Increased plasma levels of PAI-1 are implicated in the impairment of fibrinolytic function and may be associated with thrombotic diseases. Levels of PAI-1 increase with age and are elevated in conditions such as normal pregnancy and sepsis. The sensitive quantitative measurement of functionally active porcine PAI-1 (pig PAI-1) in plasma samples is easily performed with this 96 well strip format ELISA kit. The concentration level of PAI-1 activity in porcine plasma was reported to be 34 ng/ml in male and 42 ng/ml in female. The assay measures active PAI-1 in the 0.05-50 ng/ml range. Samples giving porcine PAI-1 levels above 50 ng/ml should be diluted in blocking buffer before use. It is important to ensure a platelet free preparation of plasma as platelets can release PAI-1. Functionally active PAI-1 reacts with urokinase coated onto a micro titer plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected by the assay. Cross-reactivity: 11.8percent rat PAI-1, 63.6percent rabbit PAI-1, 0percent mouse PAI-1, 100percent human PAI-1. After appropriate washing steps, anti-porcine PAI-1 primary antibody binds to the captured enzyme. Excess antibody is washed away, and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of PAI-1 in the samples. A standard calibration curve is prepared using dilutions of purified PAI-1 and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

This assay uses an exclusive enzyme capture technology to only detect functionally active protein.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, uPA Plate, Secondary Antibody

Phe-Phe-Arg-Chloromethylketone

(PPACK2)

Phe-Phe-Arg-CMK (FFRCK). Potent and specific irreversible inhibitor of plasma and glandular kallikreins. Inhibits the cleavage of atrial naturiuretic peptide (ANP 1-126) by kallikreins. Also inhibits adrenal kininogenase, a kallikrein-like enzyme.

Biotin Labeled Phe-Pro-Arg-Chloromethylketone

(PPACK-BIO)

Biotinylated Phe-Pro-Arg-CMK (FPRCK). Extremely potent and selective irreversible inhibitor of thrombin (Kobs/[I] = 107M-1S-1). Reacts with thrombin in a 1:1 stoichiometry. Can also be used to inhibit tissue plasminogen activator, urokinase, Factor VIIa, and Factor XIa.

Porcine pancreatic elastase

(PPE)

Chromatographically prepared from the euglobin fraction of porcine pancreas by the method of Lewis et al. (1) then twice crystallized. Supplied as a salt free lyophilized powder that is free of trypsin and chymotrypsin. 95percent pure by SDS-PAGE. Reconstitute with 0.05M Na Acetate 0.1M NaCl pH 5.0 to 1 mg/ml or desired concentration, aliquot and freeze remaining portion. Assay: 5-10 ug in 0.1M Tris pH 8.8 at 37 C containing 0.2mM Suc-(Al)3-pNA.
References
1. Lewis, U.J. et al. (1956) J. Biol. Chem. 22:705

Inhibitory monoclonal antibody to human prorenin, clone 4B5-E3

(PREN4B5E3)

Inhibitory mouse monoclonal antibody directed to human prorenin (Patent Pending). This antibody recognizes and binds only to prorenin. It does not bind or otherwise interact with active renin. Blocks proteolytic conversion of prorenin to renin by catalytic amounts of trypsin (unpublished data). Binding epitope is within amino acid residues 30-43 (MARLGPEWSQPMKR) of prorenin. Strongly blots prorenin under non-reducing and reducing conditions. No cross reactivity with mouse or rat prorenin. Clone 4B5-E3. IgG2b class.

Monoclonal antibody to human prorenin, biotin labeled

(PREN4B5E3-BIO)

Biotin labeled inhibitory mouse monoclonal antibody directed to human prorenin (Patent Pending). This antibody recognizes and binds only to prorenin. It does not bind or otherwise interact with active renin. Blocks proteolytic conversion of prorenin to renin by catalytic amounts of trypsin (unpublished data). Binding epitope is within amino acid residues 30-43 (MARLGPEWSQPMKR) of prorenin. Strongly blots prorenin under non-reducing and reducing conditions. No cross reactivity with mouse or rat prorenin. Clone 4B5-E3. IgG2b class.

Monoclonal antibody to human prorenin, FITC labeled

(PREN4B5E3-FITC)

FITC labeled inhibitory mouse monoclonal antibody directed to human prorenin (Patent Pending). This antibody recognizes and binds only to prorenin. It does not bind or otherwise interact with active renin. Blocks proteolytic conversion of prorenin to renin by catalytic amounts of trypsin (unpublished data). Binding epitope is within amino acid residues 30-43 (MARLGPEWSQPMKR) of prorenin. Strongly blots prorenin under non-reducing and reducing conditions. No cross reactivity with mouse or rat prorenin. Clone 4B5-E3. IgG2b class.

Monoclonal antibody to human prorenin, clone 6E2-B7

(PREN6E2B7)

Mouse monoclonal antibody directed to human prorenin. This antibody recognizes and binds to prorenin only. Binding epitope is within amino acid residues 16-29 (MPSIRESLKERGVD) of prorenin. Strongly blots prorenin under non-reducing conditions only. Cross reacts with canine prorenin due to a common epitope. No cross reactivity with mouse or rat prorenin. Clone 6E2-B7. IgG1 class.

Monoclonal antibody to human renin and prorenin, clone 7D3-E3

(PREN7D3E3)

Mouse monoclonal antibody directed to human prorenin. This antibody recognizes and binds to prorenin and renin. Binding epitope has not yet been determined but resides within the renin sequence. Clone 7D3-E3. IgG1 class.

Active porcine tPA, recombinant

(PTPA)

Recombinantly produced in insect cells. >95percent active. For ELISA and Western Blotting of pig tPA, please use our Rabbit Polyclonal Antibodies to Human tPA or our Mouse Monoclonal to Human tPA which cross react.

Active porcine tPA functional assay ELISA kit

(PTPAKT)

Tissue plasminogen activator (tPA) is a serine protease that converts plasminogen to plasmin in the blood fibrinolytic system. It also plays an important role in the nervous system, including the processes of neuronal migration, neurite outgrowth, and neuronal plasticity. tPA has been suggested to have a role in several neuropathological conditions such as cerebral ischemia, seizures, and demyelinating diseases. The sensitive quantitative measurement of functionally active porcine tPA (pig tPA) in plasma and other biological fluid samples is easily performed with this 96 well strip format ELISA kit. The basal level of tPA activity in a small sample of pigs was found to be 2 IU/ml. 1 tPA IU = 1.64 ng. The normal concentration of tPA antigen in a porcine model of cardiopulmonary bypass was 1.69 ng/ml. The concentration of tPA antigen in a porcine model of sepsis was 8.9-15.9 ng/ml depending on collection site. The assay measures active tPA in the 0.02-10 ng/ml range. Samples giving porcine tPA levels above 10 ng/ml should be diluted in blocking buffer before use. Samples of plasma in citrate or EDTA may be assayed with this kit. Plasma in heparin is not recommended. It is important to ensure a platelet free preparation of plasma as platelets can release PAI-1, which in turn could potentially form a complex with active tPA. If plasma samples were collected in citrate the pH should be brought up to neutral with the diluent provided in the kit. Serum and cell culture media at neutral pH may also be used. Functionally active tPA will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. Complexed tPA will not bind to the PAI-1 and will not be detected by the assay. After appropriate washing steps, anti-porcine tPA primary antibody binds to the captured enzyme. Excess antibody is washed away, and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of active tPA in the samples. A standard calibration curve is prepared using dilutions of purified tPA and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

This assay uses an exclusive enzyme capture technology to only detect functionally active protein.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, Avidin Plate, Secondary Antibody

Porcine multimeric vitronectin

(PVN)

Prepared from fresh porcine (pig) plasma using urea as a denaturant.

Human RAP

(RAP)

Produced recombinantly in E. Coli. Suitable to block receptor function in uptake assays. Contains endotoxin which might trigger signaling events.

FITC Labeled Human RAP

(RAP-FITC)

Produced recombinantly in E. Coli and labeled with FITC at primary amines.

Low Endotoxin Human RAP

(RAP-LE)

This preparation of Receptor Associated Protein is purified to lower levels of endotoxin. Low endotoxin RAP is suitable for experiments involving signaling events and phosphorylation studies, which may be triggered by endotoxin.

Rat Antithrombin III

(RATIII)

Prepared from fresh rat plasma using several chromatographic steps.

Rabbit Antithrombin

(RBATIII)

Prepared from fresh rabbit plasma using several chromatographic steps.

Rabbit Fibrinogen

(RbFBGN)

Prepared from fresh rabbit plasma using several chromatographic steps. Plasminogen depleted by lysine affinity chromatography. Thaw at 37C without agitation until completely liquid, then gently mix before use. Keep fibrinogen at 25-37C, aliquot and flash freeze unused portion.

FITC Labeled Rabbit Fibrinogen

(RbFBGN-FITC)

Rabbit fibrinogen amino terminal labeled with fluorescein isothiocyanate (FITC). Prepared from fresh rabbit plasma using several chromatographic steps. Plasminogen depleted by lysine affinity chromatography. Thaw at 37C without agitation until completely liquid, then gently mix before use. Keep fibrinogen at 25-37C, aliquot and flash freeze unused portion.

Rabbit IgG, Protein A Purified

(RB-GF)

Purified from normal serum by immobilized Protein A. >95 percent pure by SDS-PAGE and preservative free.

Biotin labeled rabbit IgG

(RB-GF-BIO)

Rabbit IgG purified from normal serum by immobilized Protein A then biotin labeled. >95 percent pure by SDS-PAGE and preservative free.

Low Endotoxin Rabbit IgG, Protein A Purified

(RB-GF-ED)

Purified from normal serum by immobilized Protein A using low endotoxin methodology and sterile filtered. >95 percent pure by SDS-PAGE and preservative free. Add deionized water to original volume, aliquot and freeze unused portion.

Rabbit PAI-1 (wild type active)

(RbPAI)

Rabbit PAI-1 is now available and is preferred for animal studies that utilize rabbit models. Wild type rabbit PAI-1 is less than 20percent active and may not be suitable for most experiments. As a replacement please consider rabbit PAI-1 stable mutant (Catalog Number RbPAI-I91L) which is 70-80percent active.

Rabbit PAI-1 (stable mutant)

(RbPAI-I91L)

This stable mutant of rabbit PAI-1 contains a single conservative mutation (Isoleucine 91 to Leucine) that yields PAI-1 with an enhanced half-life as compared to the wild type.

Active rabbit PAI-1 functional assay ELISA kit

(RbPAIKT)

Plasminogen activator inhibitor type 1 (PAI-1) is involved in the regulation of the blood fibrinolytic system. Increased plasma levels of PAI-1 are implicated in the impairment of fibrinolytic function and may be associated with thrombotic diseases. Levels of PAI-1 increase with age and are elevated in conditions such as normal pregnancy and sepsis. The sensitive quantitative measurement of functionally active rabbit PAI-1 in plasma samples is easily performed with this 96 well strip format ELISA kit. The concentration of PAI-1 activity was reported to be 20.5 ng/ml in rabbit plasma and 2.9 ng/ml in rabbit serum. The assay measures active PAI-1 in the 0.05-50 ng/ml range. Samples giving rabbit PAI-1 levels above 50 ng/ml should be diluted in blocking buffer before use. It is important to ensure a platelet free preparation of plasma as platelets can release PAI-1. Functionally active PAI-1 reacts with urokinase coated onto a micro titer plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected by the assay. After appropriate washing steps, anti-rabbit PAI-1 primary antibody binds to the captured enzyme. Excess antibody is washed away, and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of PAI-1 in the samples. A standard calibration curve is prepared using dilutions of purified PAI-1 and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

This assay uses an exclusive enzyme capture technology to only detect functionally active protein.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, uPA Plate, Secondary Antibody

Rabbit PAI-1 (wild type latent)

(RbPAI-L)

Latent rabbit PAI-1 is a useful control for experiments when used in conjunction with the active form.

Rabbit PAI-1 (Biotin labeled latent fraction)

(RbPAI-L-BIO)

This latent fraction of recombinant rabbit PAI-1 is biotin labeled on lysine residues.

Rabbit PAI-1 depleted plasma, sodium citrate

(RbPLA-SC-PAI)

Prepared from frozen rabbit plasma using immobilized anti-rabbit PAI-1 IgG.

Rabbit plasminogen

(RbPLG)

Prepared from fresh rabbit plasma by immobilized lysine chromatography.

Rabbit plasmin

(RbPLM)

Prepared from plasminogen by activation with immobilized human uPA. 100 percent functionally active plasmin is purified from the activation reaction by immobilized SBTI. Plasmin will undergo rapid auto proteolysis in the absence of benzamidine and should be used quickly once thawed. Aliquot to avoid freeze-thaw cycles.

Active rabbit tPA, recombinant

(RbTPA)

Rabbit tPA recombinantly produced in insect cells which is >95percent active.

Active rabbit tPA functional assay ELISA kit

(RbTPAKT)

Tissue plasminogen activator (tPA) is a serine protease that converts plasminogen to plasmin in the blood fibrinolytic system. It also plays an important role in the nervous system, including the processes of neuronal migration, neurite outgrowth, and neuronal plasticity. tPA has been suggested to have a role in several neuropathological conditions such as cerebral ischemia, seizures, and demyelinating diseases. The sensitive quantitative measurement of functionally active rabbit tPA in plasma and other biological fluid samples is easily performed with this 96 well strip format ELISA kit. In-house testing of pooled normal rabbit plasma in citrate indicates a tPA concentration of 0.5-1 ng/ml. The assay measures active tPA in the 0.05-20 ng/ml range. Samples giving porcine tPA levels above 20 ng/ml should be diluted in blocking buffer before use. Samples of plasma in citrate or EDTA may be assayed with this kit. Plasma in heparin is not recommended. It is important to ensure a platelet free preparation of plasma as platelets can release PAI-1, which in turn could potentially form a complex with active tPA. If plasma samples were collected in citrate the pH should be brought up to neutral with the 10X TBS provided in the kit. Serum and cell culture media at neutral pH may also be used. Functionally active tPA will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. Complexed tPA will not bind to the PAI-1 and will not be detected by the assay. After appropriate washing steps, anti-rabbit tPA primary antibody binds to the captured enzyme. Excess antibody is washed away, and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of active tPA in the samples. A standard calibration curve is prepared using dilutions of purified tPA and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

This assay uses an exclusive enzyme capture technology to only detect functionally active protein.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, Avidin Plate

Active Rabbit Urokinase

(RbUPA)

Active two-chain rabbit urokinase (uPA), recombinantly produced in insect cells.

Rabbit multimeric vitronectin

(RbVN)

Prepared from fresh rabbit plasma using urea as a denaturant.

Rat Fibrinogen

(RFBGN)

Prepared from fresh rat plasma using several chromatographic steps. Plasminogen depleted by lysine affinity chromatography. Thaw protein in a 37C water bath. Aliquot and freeze the unused portion. Keep fibrinogen at 25-37C, aliquot and flash freeze unused portion.

FITC Labeled Rat Fibrinogen

(RFBGN-FITC)

Rat fibrinogen amino terminal labeled with fluorescein isothiocyanate (FITC). Prepared from fresh rat plasma using several chromatographic steps. Plasminogen depleted by lysine affinity chromatography. Thaw protein in a 37C water bath. Aliquot and freeze the unused portion. Keep fibrinogen at 25-37C, aliquot and flash freeze unused portion.

Rat fibrinogen total antigen assay ELISA kit

(RFBGNKT)

Fibrinogen is a soluble glycoprotein that circulates in the blood and is converted to insoluble fibrin by thrombin in the final step of the coagulation cascade. Hepatic expression of fibrinogen increases two to four hundred fold during the acute phase response to infection or inflammation. Elevated fibrinogen levels are correlated with cardiovascular disease and atherosclerosis. The sensitive quantitative measurement of rat fibrinogen in plasma and serum samples is easily performed with this 96 well strip format ELISA kit. Fibrinogen is present in normal rat plasma at a concentration of 3.1 mg/ml and varies by age and diet. The assay measures total rat fibrinogen in the 3.125-800 ng/ml range. Samples giving rat fibrinogen levels above 800 ng/ml should be diluted in 1X diluent before use. A 1:10,000 to 1:50,000 dilution for plasma or serum is suggested for best results. Rat fibrinogen will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-rat fibrinogen primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated avidin. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of fibrinogen in the samples. A standard calibration curve is prepared using dilutions of purified fibrinogen and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, Rat Fibrinogen Antigen Capture Plate, Avidin-HRP

Rat Prothrombin

(RFII)

Rat prothrombin is prepared from fresh frozen rat plasma. Purity is determined by SDS-PAGE analysis.

Rat Factor IX

(RFIX)

Rat factor IX is prepared from fresh frozen plasma by a combination of conventional procedures and immunoaffinity chromatography. Purity is determined by SDS-PAGE analysis and activity is measured using a factor IX clotting assay.

Rat Factor IXa

(RFIXA)

Factor IXa is prepared from highly purified factor IX by activation with factor XIa. The factor IXa is further purified by gel filtration, followed by immunoaffinity purification. Purity is assessed by SDS-PAGE analysis. Activity is determined in a one-stage clotting assay.

Rat Factor X

(RFX)

Rat factor X is prepared from fresh frozen plasma by a combination of conventional procedures and immunoaffinity chromatography. Purity is determined by SDS-PAGE analysis and activity is measured using a factor X clotting assay.

Rat Factor Xa

(RFXA)

Factor Xa is prepared from homogenous Factor X by activation with Russell’s viper venom. The venom is removed after activation. Purity is determined by SDS-PAGE analysis and activity is measured in a factor Xa clotting assay and/or chromogenic substrate assay. Thaw rapidly in a 37C water bath without allowing protein to warm to 37C and immediately cool on ice.

Rat PAI-1 (wild type active form)

(RPAI)

Recombinant rat PAI-1 is the ideal choice for studies involving this animal model of fibrinolysis and cancer studies. The active fraction typically contains 95percent active PAI-1.

Rat PAI-1 (stable mutant)

(RPAI-I91L)

This recombinant rat PAI-1 has a single conservative mutation (Isoleucine 91 to Leucine) that yields a rat PAI-1 with an enhanced half-life as compared to the wild type.

Rat PAI-1 (Biotin labeled stable mutant)

(RPAI-I91L-BIO)

This recombinant rat PAI-1 has a single conservative mutation (Isoleucine 91 to Leucine) for increased half-life and is biotin labeled on lysine residues.

Active rat PAI-1 functional assay ELISA kit

(RPAIKT)

Plasminogen activator inhibitor type 1 (PAI-1) is involved in the regulation of the blood fibrinolytic system. Increased plasma levels of PAI-1 are implicated in the impairment of fibrinolytic function and may be associated with thrombotic diseases. Levels of PAI-1 increase with age and are elevated in conditions such as normal pregnancy and sepsis. The sensitive quantitative measurement of functionally active rat PAI-1 in plasma samples is easily performed with this 96 well strip format ELISA kit. The concentration level of PAI-1 activity in rat plasma was found to be 1.0 ng/ml. The assay measures active PAI-1 in the 0.05-50 ng/ml range. Samples giving rat PAI-1 levels above 50 ng/ml should be diluted in blocking buffer before use. It is important to ensure a platelet free preparation of plasma as platelets can release PAI-1. Functionally active PAI-1 reacts with urokinase coated onto a micro titer plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected by the assay. Cross-reactivity: 12percent porcine PAI-1, 100percent mouse PAI-1. After appropriate washing steps, anti-rat PAI-1 primary antibody binds to the captured enzyme. Excess antibody is washed away, and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of PAI-1 in the samples. A standard calibration curve is prepared using dilutions of purified PAI-1 and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

This assay uses an exclusive enzyme capture technology to only detect functionally active protein.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, uPA Plate, Secondary Antibody

Rat PAI-1 total antigen assay ELISA kit

(RPAIKT-TOT)

Plasminogen activator inhibitor type 1 (PAI-1) is involved in the regulation of the blood fibrinolytic system. Increased plasma levels of PAI-1 are implicated in the impairment of fibrinolytic function and may be associated with thrombotic diseases. Levels of PAI-1 increase with age and are elevated in conditions such as normal pregnancy and sepsis. The sensitive quantitative measurement of total rat PAI-1 antigen in plasma samples is easily performed with this 96 well strip format ELISA kit. The concentration level of PAI-1 antigen in rat plasma was found to be 1.8 ng/ml. The assay measures rat PAI-1 in the 0.05-50 ng/ml range. Samples giving rat PAI-1 levels above 50 ng/ml should be diluted in blocking buffer before use. Normal plasma should be applied directly to the plate for best results. It is important to ensure a platelet free preparation of plasma as platelets can release PAI-1. Rat PAI-1 will bind to the capture antibody coated on the microtiter plate. Free, latent, and complexed PAI-1 will be detected by the assay. After appropriate washing steps, anti-rat PAI-1 primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of rat PAI-1 in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified rat PAI-1 and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, Rat PAI-1 Antigen Capture Plate, Secondary Antibody

Rat PAI-1 (wild type latent form)

(RPAI-L)

Latent rat PAI-1 is a useful control for experiments when used in conjunction with the active form or stable mutant.

Rat PAI-1 (Biotin labeled latent fraction)

(RPAI-L-BIO)

Biotin labeled latent rat PAI-1 is a useful control for experiments involving the biotinylated stable mutant.

Rat PAI-1 Antigen Capture Plate

(RPAI-TOT-PLATE)

96 well plate with 8 removable strips coated with monoclonal antibody to rat Plasminogen Activator Inhibitor (PAI-1) at 100 ul/well and blocked with 300 ul/well. Ideal for specific binding of rat PAI-1 antigen for sandwich style ELISA experiments.

Rat pancreatic elastase

(RPE)

Chromatographically prepared from the euglobin fraction of pancreas by the method of Lewis et al. (1) then twice crystallized. Supplied as salt free lyophilized powder. The protein is stable 9-12 months when stored at 5 C and does not contain trypsin or chymotrypsin. 95percent pure by SDS-PAGE. Reconstitute with 0.05M Na Acetate 0.2M NaCl pH 5.0 to 1 mg/ml or desired concentration, aliquot and freeze remaining portion.
Assay: 5-10 ug in 0.1M Tris pH 8.8 at 37 C containing 0.2mM Suc-(Al)3-pNA.
References: 1. Lewis, U.J. et al. (1956) J. Biol. Chem. 22:705

Rat Latent PAI-1 depleted plasma, sodium citrate

(RPLA-SC-L)

Prepared from frozen rat plasma using immobilized anti-rat PAI-1 IgG.

Rat PAI-1 depleted plasma, sodium citrate

(RPLA-SC-PAI)

Prepared from frozen rat plasma using immobilized anti-rat PAI-1 IgG.

Rat Renin/Prorenin Double Depleted Plasma

(RPLA-SC-PREN)

Prepared from frozen rat plasma using immobilized anti-human Renin IgG.

Rat plasminogen

(RPLG)

Prepared from fresh rat plasma by immobilized lysine chromatography.

Rat plasmin

(RPLM)

Prepared from plasminogen by activation with immobilized human uPA. 100 percent functionally active plasmin is purified from the activation reaction by immobilized SBTI. Plasmin will undergo rapid auto proteolysis in the absence of benzamidine and should be used quickly once thawed. Aliquot to avoid freeze-thaw cycles.

Rat Prorenin, C-terminal 8x His tag

(RPREN-HIS)

Recombinantly produced in HEK cell culture and purified by chelated metal affinity chromatography. Contains a 8X-Histidine tag at C terminus for purification. Fully activatable to renin by catalytic amounts of trypsin. Prorenin is a glycosylated aspartic protease that consists of 2 homologous lobes and is the precursor of renin. Prorenin exhibits a low level of enzymatic activity relative to renin which is generated from prorenin by proteolytic cleavage of the first ~43 amino acids at the N-terminus. This so called prosegment appears to block the full enzymatic potential of the active site (1). Renin activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE, the angiotensin-converting enzyme primarily within the capillaries of the lungs. It has been reported that the levels of circulating prorenin (but not renin) are increased in diabetic subjects(2).

1) A.H. Jan Danser; Jaap Deinum ; Renin, Prorenin and the Putative (Pro)renin Receptor). Hypertension. 2005;46:1069.

2) Luetscher JA, Kraemer FB, Wilson DM, Schwartz HC, Bryer-Ash M. Increased plasma inactive renin in diabetes mellitus. A marker of microvascular complications. N Engl J Med. 1985;312:1412-1417.

Rat Prorenin/Renin Total Antigen ELISA Kit

(RPRENKT-TOT)

Prorenin is a glycosylated aspartic protease that consists of 2 homologous lobes and is the precursor of renin. Renin activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE, the angiotensin-converting enzyme primarily within the capillaries of the lungs. It has been reported that the levels of circulating prorenin (but not renin) are increased in diabetic subjects. Plasma and serum concentrations increase in several conditions such as pregnancy, progressive diabetes mellitus, diabetes mellitus with microvascular disease, and diabetic retinopathy. The sensitive quantitative measurement of total rat prorenin and renin antigen in plasma, serum or cell culture supernatant samples is easily performed with this 96 well strip format ELISA kit. Renin and prorenin will be detected by the assay. Rat prorenin levels range from 0-400 ng/ml depending on assay methodology. The assay measures total rat prorenin in the 0.1-100 ng/ml range. Samples giving rat prorenin levels above 100 ng/ml should be diluted in blocking buffer before use. Rat prorenin will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-rat prorenin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with avidin conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of prorenin in the samples. A standard calibration curve is prepared using dilutions of purified prorenin and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Rhesus Monkey IgG, Protein A Purified

(RS-GF)

Purified from normal serum by immobilized Protein A. >95 percent pure by SDS-PAGE and preservative free.

Rat Thrombin

(RTHROM)

Rat thrombin is prepared from purified rat prothrombin by activation with Russells Viper Venom. This venom is removed after activation. Purity is >90percent as determined by SDS-PAGE analysis. Activity is measured in a thrombin specific clotting assay, and compared to standardized NIH thrombin.

Active rat tPA, recombinant

(RTPA)

Recombinantly produced in insect cells. >85percent single chain, >95percent active.

Active rat tPA functional assay ELISA kit

(RTPAKT)

Tissue plasminogen activator (tPA) is a serine protease that converts plasminogen to plasmin in the blood fibrinolytic system. It also plays an important role in the nervous system, including the processes of neuronal migration, neurite outgrowth, and neuronal plasticity. tPA has been suggested to have a role in several neuropathological conditions such as cerebral ischemia, seizures, and demyelinating diseases. The sensitive quantitative measurement of functionally active mouse tPA in plasma and other biological fluid samples is easily performed with this 96 well strip format ELISA kit. The level of active tPA in normal rat plasma was found to be 1.6 ng/ml. The assay measures active tPA in the 0.1-50 ng/ml range. Samples giving rat tPA levels above 50 ng/ml should be diluted in blocking buffer before use. Samples of plasma in citrate or EDTA may be assayed with this kit. Plasma in heparin is not recommended. It is important to ensure a platelet free preparation of plasma as platelets can release PAI-1, which in turn could potentially form a complex with active tPA. If plasma samples were collected in citrate the pH should be brought up to neutral with the 10X TBS provided in the kit. Serum and cell culture media at neutral pH may also be used. Functionally active tPA will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. Complexed tPA will not bind to the PAI-1 and will not be detected by the assay. After appropriate washing steps, anti-rat tPA primary antibody binds to the captured enzyme. Excess antibody is washed away, and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of active tPA in the samples. A standard calibration curve is prepared using dilutions of purified tPA and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

This assay uses an exclusive enzyme capture technology to only detect functionally active protein.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, Avidin Plate, Secondary Antibody

Rat tPA total antigen assay ELISA kit

(RTPAKT-TOT)

Tissue plasminogen activator (tPA) is a serine protease that converts plasminogen to plasmin in the blood fibrinolytic system. It also plays an important role in the nervous system, including the processes of neuronal migration, neurite outgrowth, and neuronal plasticity. tPA has been suggested to have a role in several neuropathological conditions such as cerebral ischemia, seizures, and demyelinating diseases. The sensitive quantitative measurement of total rat tPA antigen in plasma and other biological fluid samples is easily performed with this 96 well strip format ELISA kit. The level of active tPA in normal rat plasma was found to be 2.5 ng/ml. The assay measures total tPA in the 0.1-50 ng/ml range. Samples giving rat tPA levels above 50 ng/ml should be diluted in blocking buffer before use. Rat tPA will bind to the capture antibody coated on the microtiter plate. Free and complexed tPA will be detected by the assay. After appropriate washing steps, anti-rat tPA primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of rat tPA in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified rat tPA and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, tPA Antigen Capture Plate, Secondary Antibody

Active rat tPA, two chain recombinant

(RTPA-TC)

Recombinantly produced in insect cells. Activated from single-chain form with immobilized plasmin. >95percent two chain, >95percent active.

Active Rat Urokinase, HMW

(RUPA)

Active two-chain HMW rat urokinase, recombinantly produced in insect cells.

Active rat uPA functional assay ELISA kit

(RUPAKT)

Urokinase plasminogen activator (uPA) is a serine protease that activates plasminogen to plasmin in the blood fibrinolytic system. It is also implicated in events related to cell invasion/migrations. The sensitive quantitative measurement of functionally active rat uPA in plasma and other biological fluid samples is easily performed with this 96 well strip format ELISA kit. The assay measures active uPA in the 0.05-10 ng/ml range. Samples giving rat uPA levels above 10 ng/ml should be diluted in blocking buffer before use. It is important to ensure a platelet free preparation of plasma as platelets can release PAI-1, which in turn could potentially form a complex with active uPA. If plasma samples were collected in citrate the pH should be brought up to neutral with 10X TBS. Serum and cell culture media at neutral pH may also be used. If using kidney extracts that have been extracted using triton X, dialyze to remove the triton X before using in the assay. Detergents such as triton X may interfere with the assay. Functionally active uPA will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. Inactive or complexed uPA will not bind to the PAI-1 and will not be detected by the assay. After appropriate washing steps, anti-rat uPA primary antibody binds to the captured enzyme. Excess antibody is washed away, and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of active uPA in the samples. A standard calibration curve is prepared using dilutions of purified uPA and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, Avidin Plate, Secondary Antibody

Rat uPA total antigen assay ELISA kit

(RUPAKT-TOT)

Urokinase plasminogen activator (uPA) is a serine protease that activates plasminogen to plasmin in the blood fibrinolytic system. It is also implicated in events related to cell invasion/migrations. The sensitive quantitative measurement of total rat uPA antigen in plasma and other biological fluid samples is easily performed with this 96 well strip format ELISA kit. The assay measures total uPA in the 0.01-10 ng/ml range. Samples giving rat uPA levels above 10 ng/ml should be diluted in blocking buffer before use. Rat uPA will bind to the capture antibody coated on the microtiter plate. Free and complexed uPA will be detected by the assay. After appropriate washing steps, anti-rat uPA primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of rat uPA in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified rat uPA and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
Suggested additional reagents: 10X Wash Buffer, TMB Substrate, uPA Antigen Capture Plate, Secondary Antibody

Rat multimeric vitronectin

(RVN)

Prepared from fresh rat plasma using urea as a denaturant.

Russells Viper Venom Factor V Activator

(RVV-V)

Purified from Russells Viper Venom. Serine protease that activates single chain Factor V to two chain active Va. This protease is inhibited by diisopropylfluorophosphate (DFP). RVV-V is a single chain glycoprotein and is highly pure and homogenous by SDS-PAGE.

Russells Viper Venom Factor X Activator

(RVV-X)

Purified from Russells Viper Venom. Zinc dependant endopeptidase that activates Factor X to Xa and Factor IX to IXa-alpha. This protease is inhibited by EDTA and o-phenanthroline. RVV-X is a glycoprotein that consists of two disulfide linked subunits of molecular weights 67kD and 26kD and is highly pure and homogenous by SDS-PAGE.

Human PAI-1 (NBD labeled vitronectin reporter mutant latent fraction)

(S119C-L-NBD)

This mutant contains a Ser to Cys replacement around the vitronectin binding site. Incorporation of an NBD labels allows for the reporting of binding to vitronectin. This latent fraction is an ideal control for experiments with S119C-NBD.

Human PAI-1 (NBD labeled vitronectin reporter mutant)

(S119C-NBD)

This mutant contains a Ser to Cys replacement around the vitronectin binding site. Incorporation of an NBD labels allows for the reporting of binding to vitronectin.

Sheep anti dog uPA antiserum

(SASDUPA)

Polyclonal antiserum (host sheep).

Sheep anti dog uPA IgG fraction

(SASDUPA-GF)

Polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti dog uPA IgG fraction, biotin labeled

(SASDUPA-GF-BIO)

Biotin labeled polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti dog uPA IgG fraction, FITC labeled

(SASDUPA-GF-FITC)

FITC labeled polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti dog uPA IgG fraction, HRP labeled

(SASDUPA-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti-Heparin Cofactor II

(SASHCII-GF)

Polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti-Human Prothrombin

(SASHFII-GF)

Polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti-Human Prothrombin – Affinity Purified

(SASHFII-GF-HT)

Polyclonal antibody (host sheep). Affinity purified by immobilized human prothrombin.

Sheep anti human Factor IX antiserum

(SASHFIX)

Polyclonal antiserum (host sheep).

Sheep anti-Human Factor IX IgG Fraction

(SASHFIX-GF)

Polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti-Human Factor V

(SASHFV-GF)

Polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti-Human Factor VII

(SASHFVII-GF)

Polyclonal antibody to Human Factor VII. Recognizes Human Factor VII and VIIa by ELISA and Western Blot.

Sheep anti-Human Factor VIII

(SASHFVIII-GF)

Polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti-Human Factor X

(SASHFX-GF)

Polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti human factor XII antiserum

(SASHFXII)

Polyclonal antiserum (host sheep) to human factor XII.

Sheep anti human factor XII IgG fraction

(SASHFXII-GF)

Polyclonal antibody (host sheep) to human factor XII. IgG fraction purified by immobilized Protein G.

Sheep anti human factor XII IgG fraction, biotin labeled

(SASHFXII-GF-BIO)

Biotin labeled polyclonal antibody (host sheep) to human factor XII. IgG fraction purified by immobilized Protein G.

Sheep anti human factor XII IgG fraction, FITC labeled

(SASHFXII-GF-FITC)

FITC labeled polyclonal antibody (host sheep) to human factor XII. IgG fraction purified by immobilized Protein G.

Sheep anti human factor XII IgG fraction, HRP labeled

(SASHFXII-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host sheep) to human factor XII. IgG fraction purified by immobilized Protein G.

Sheep anti-Human Factor XIII

(SASHFXIII-GF)

Polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti human PAI-1 antiserum

(SASHPAI)

Polyclonal antiserum (host sheep).

Sheep anti human PAI-1 IgG fraction

(SASHPAI-GF)

Polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Biotin labeled sheep anti human PAI-1 IgG

(SASHPAI-GF-BIO)

Biotin labeled polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti human PAI-1 IgG fraction, FITC labeled

(SASHPAI-GF-FITC)

FITC labeled polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti human PAI-1 IgG fraction, HRP labeled

(SASHPAI-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti-human PAI-1, affinity purified

(SASHPAI-GF-HT)

Polyclonal antibody (host sheep). Affinity purified by immobilized human PAI-1.

Sheep anti-Human Protein C

(SASHPC-GF)

Polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti human plasminogen antiserum

(SASHPLG)

Polyclonal antiserum (host sheep).

Sheep anti human plasminogen IgG fraction

(SASHPLG-GF)

Polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti human plasminogen IgG fraction, biotin labeled

(SASHPLG-GF-BIO)

Biotin labeled polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti human plasminogen IgG fraction, FITC labeled

(SASHPLG-GF-FITC)

FITC labeled polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti human plasminogen IgG fraction, HRP labeled

(SASHPLG-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti-human plasminogen, affinity purified

(SASHPLG-GF-HT)

Polyclonal antibody (host sheep). Affinity purified by immobilized human plasminogen.

Sheep anti-human plasmin, affinity purified

(SASHPLM-GF-HT)

Polyclonal antibody (host sheep). Affinity purified by immobilized human plasmin coupled by the active site.

Sheep anti-Human Protein S

(SASHPS-GF)

Polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti-Human Protein Z

(SASHPZ-GF)

Polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti human RAP antiserum

(SASHRAP)

Polyclonal antiserum (host sheep).

Sheep anti human RAP IgG fraction

(SASHRAP-GF)

Polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti human RAP IgG fraction, biotin labeled

(SASHRAP-GF-BIO)

Biotin labeled polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti human RAP IgG fraction, FITC labeled

(SASHRAP-GF-FITC)

FITC labeled polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti human RAP IgG fraction, HRP labeled

(SASHRAP-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti human renin antiserum

(SASHREN)

Polyclonal antiserum (host sheep) to human renin.

Sheep anti human renin IgG fraction

(SASHREN-GF)

Polyclonal antibody (host sheep) to human renin. IgG fraction purified by immobilized Protein G.

Sheep anti human renin IgG fraction, biotin labeled

(SASHREN-GF-BIO)

Biotin labeled polyclonal antibody (host sheep) to human renin. IgG fraction purified by immobilized Protein G.

Sheep anti human renin IgG fraction, FITC labeled

(SASHREN-GF-FITC)

FITC labeled polyclonal antibody (host sheep) to human renin. IgG fraction purified by immobilized Protein G.

Sheep anti human renin IgG fraction, HRP labeled

(SASHREN-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host sheep) to human renin. IgG fraction purified by immobilized Protein G.

Sheep anti human renin IgG fraction, affinity purified

(SASHREN-GF-HT)

Polyclonal antibody (host sheep) to human renin. Affinity purified by immobilized human prorenin. Also available immobilized to agarose resin.

Biotin labeled affinity purified sheep anti human renin

(SASHREN-GF-HT-BIO)

Biotin labeled polyclonal antibody (host sheep) to human renin. Affinity purified by immobilized human prorenin.

Sheep anti-Human TAFI

(SASHTAFI-GF)

Polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti-Human Tissue Factor

(SASHTF-GF)

Polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti-Human Tissue Factor Pathway Inhibitor

(SASHTFPI-GF)

Polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti-Human Thrombin

(SASHTHROM-GF)

Polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti human tPA antiserum

(SASHTPA)

Polyclonal antiserum (host sheep).

Sheep anti human tPA IgG fraction

(SASHTPA-GF)

Polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti human tPA IgG fraction, biotin labeled

(SASHTPA-GF-BIO)

Biotin labeled polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti human tPA IgG fraction, FITC labeled

(SASHTPA-GF-FITC)

FITC labeled polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti human tPA IgG fraction, HRP labeled

(SASHTPA-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti human uPA antiserum

(SASHUPA)

Polyclonal antiserum (host sheep).

Sheep anti human uPA IgG fraction

(SASHUPA-GF)

Polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti human uPA IgG fraction, biotin labeled

(SASHUPA-GF-BIO)

Biotin labeled polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti human uPA IgG fraction, FITC labeled

(SASHUPA-GF-FITC)

FITC labeled polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti human uPA IgG fraction, HRP labeled

(SASHUPA-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti-Human vWF

(SASHVWF-GF)

Polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti human ZPI antiserum

(SASHZPI)

Polyclonal antiserum (host sheep).

Sheep anti human ZPI IgG fraction

(SASHZPI-GF)

Polyclonal antibody (host sheep) to human Protein Z-Dependent Protease Inhibitor. IgG fraction purified by immobilized Protein G.

Sheep anti human ZPI IgG fraction, biotin labeled

(SASHZPI-GF-BIO)

Biotin labeled polyclonal antibody (host sheep) to human Protein Z-Dependent Protease Inhibitor. IgG fraction purified by immobilized Protein G.

Sheep anti human ZPI IgG fraction, FITC labeled

(SASHZPI-GF-FITC)

FITC labeled polyclonal antibody (host sheep) to human Protein Z-Dependent Protease Inhibitor. IgG fraction purified by immobilized Protein G.

Sheep anti human ZPI IgG fraction, HRP labeled

(SASHZPI-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host sheep) to human Protein Z-Dependent Protease Inhibitor. IgG fraction purified by immobilized Protein G.

Sheep anti mouse factor IX antiserum

(SASMFIX)

Polyclonal antiserum (host sheep) to mouse factor IX.

Sheep anti mouse factor IX IgG fraction

(SASMFIX-GF)

Polyclonal antibody (host sheep) to mouse factor IX. IgG fraction purified by immobilized Protein G.

Biotin Labeled Anti mouse factor IX

(SASMFIX-GF-BIO)

Biotin labeled polyclonal antibody (host sheep) to mouse factor IX. IgG fraction purified by immobilized Protein G.

FITC Labeled Anti mouse factor IX

(SASMFIX-GF-FITC)

FITC labeled polyclonal antibody (host sheep) to mouse factor IX. IgG fraction purified by immobilized Protein G.

Sheep anti mouse Factor IX IgG fraction, HRP labeled

(SASMFIX-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host sheep) to mouse factor IX. IgG fraction purified by immobilized Protein G.

Sheep anti mouse factor X antiserum

(SASMFX)

Polyclonal antiserum (host sheep) to mouse factor X.

Sheep anti mouse factor X IgG fraction

(SASMFX-GF)

Polyclonal antibody (host sheep) to mouse factor X. IgG fraction purified by immobilized Protein G.

Biotin Labeled Anti mouse factor X

(SASMFX-GF-BIO)

Biotin labeled polyclonal antibody (host sheep) to mouse factor X. IgG fraction purified by immobilized Protein G.

FITC Labeled Anti mouse factor X

(SASMFX-GF-FITC)

FITC labeled polyclonal antibody (host sheep) to mouse factor X. IgG fraction purified by immobilized Protein G.

HRP Labeled Anti mouse factor X

(SASMFX-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host sheep) to mouse factor X. IgG fraction purified by immobilized Protein G.

Affinity purified sheep anti mouse factor X

(SASMFX-GF-HT)

Polyclonal antibody (host sheep). Affinity purified by immobilized mouse factor X.

Immuno absorbed sheep anti mouse Factor X

(SASMFX-GF-IA)

Polyclonal antibody to mouse Factor X (Immuno absorbed against human Factor X).

Sheep anti mouse neuroserpin antiserum

(SASMNSP)

Polyclonal antiserum (host sheep) to mouse neuroserpin (His Tag).

Sheep anti mouse neuroserpin IgG fraction

(SASMNSP-GF)

Polyclonal antibody (host sheep) to mouse neuroserpin (His Tag). IgG fraction purified by immobilized Protein G.

Biotin labeled sheep anti mouse neuroserpin

(SASMNSP-GF-BIO)

Biotin labeled polyclonal antibody (host sheep) to mouse neuroserpin (His Tag). IgG fraction purified by immobilized Protein G.

FITC labeled sheep anti mouse neuroserpin

(SASMNSP-GF-FITC)

FITC labeled polyclonal antibody (host sheep) to mouse neuroserpin (His Tag). IgG fraction purified by immobilized Protein G.

Sheep anti mouse neuroserpin IgG fraction, HRP labeled

(SASMNSP-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host sheep) to mouse neuroserpin (His Tag). IgG fraction purified by immobilized Protein G.

Affinity purified sheep anti mouse neuroserpin

(SASMNSP-GF-HT)

Polyclonal antibody (host sheep) to mouse neuroserpin (His Tag). Affinity purified by immobilized mouse neuroserpin.

Biotin labeled affinity purified sheep anti mouse neuroserpin

(SASMNSP-GF-HT-BIO)

Biotin labeled affinity purified polyclonal antibody (host sheep) to mouse neuroserpin (His Tag). Affinity purified by immobilized mouse neuroserpin followed by biotin conjugation.

Sheep anti mouse Protein C antiserum

(SASMPC)

Polyclonal antiserum (host sheep) to mouse protein C and APC.

Sheep anti mouse Protein C IgG fraction

(SASMPC-GF)

Polyclonal antibody (host sheep) to mouse protein C and APC. IgG fraction purified by immobilized Protein G.

Sheep anti mouse Protein C IgG fraction, biotin labeled

(SASMPC-GF-BIO)

Biotin labeled polyclonal antibody (host sheep) to mouse protein C and APC. IgG fraction purified by immobilized Protein G.

Sheep anti mouse Protein C IgG fraction, FITC labeled

(SASMPC-GF-FITC)

FITC labeled polyclonal antibody (host sheep) to mouse protein C and APC. IgG fraction purified by immobilized Protein G.

Sheep anti mouse Protein C IgG fraction, HRP labeled

(SASMPC-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host sheep) to mouse protein C and APC. IgG fraction purified by immobilized Protein G.

Sheep anti mouse Protease Nexin 1 antiserum

(SASMPN1)

Polyclonal antiserum (host sheep).

Sheep anti mouse Protease Nexin 1 IgG fraction

(SASMPN1-GF)

Polyclonal antibody (host sheep) to mouse protease nexin 1. IgG fraction purified by immobilized Protein G.

Sheep anti mouse Protease Nexin 1 IgG fraction, biotin labeled

(SASMPN1-GF-BIO)

Biotin labeled polyclonal antibody (host sheep) to mouse protease nexin 1. IgG fraction purified by immobilized Protein G.

Sheep anti mouse Protease Nexin 1 IgG fraction, FITC labeled

(SASMPN1-GF-FITC)

FITC labeled polyclonal antibody (host sheep) to mouse protease nexin 1. IgG fraction purified by immobilized Protein G.

Sheep anti mouse Protease Nexin 1 IgG fraction, HRP labeled

(SASMPN1-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host sheep) to mouse protease nexin 1. IgG fraction purified by immobilized Protein G.

Sheep anti mouse prothrombin antiserum

(SASMPT)

Polyclonal antiserum (host sheep) to mouse prothrombin and thrombin.

Sheep anti mouse prothrombin IgG fraction

(SASMPT-GF)

Polyclonal antibody (host sheep) to mouse prothrombin and thrombin. IgG fraction purified by immobilized Protein G.

Sheep anti mouse prothrombin IgG fraction, biotin labeled

(SASMPT-GF-BIO)

Biotin labeled polyclonal antibody (host sheep) to mouse prothrombin and thrombin. IgG fraction purified by immobilized Protein G.

Sheep anti mouse prothrombin IgG fraction, FITC labeled

(SASMPT-GF-FITC)

FITC labeled polyclonal antibody (host sheep) to mouse prothrombin and thrombin. IgG fraction purified by immobilized Protein G.

Sheep anti mouse prothrombin IgG fraction, HRP labeled

(SASMPT-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host sheep) to mouse prothrombin and thrombin. IgG fraction purified by immobilized Protein G.

Sheep anti mouse prothrombin IgG fraction, affinity purified

(SASMPT-GF-HT)

Polyclonal antibody (host sheep). Affinity purified by immobilized mouse prothrombin.

Sheep anti mouse prothrombin, biotin labeled affinity purified

(SASMPT-GF-HT-BIO)

Biotin labeled polyclonal antibody (host sheep). Affinity purified by immobilized mouse prothrombin.

Sheep Anti Mouse and Rat tPA antiserum

(SASMTPA)

Polyclonal antiserum (host sheep).

Sheep Anti Mouse and Rat tPA IgG fraction

(SASMTPA-GF)

Polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep Anti Mouse and Rat tPA IgG fraction, biotin labeled

(SASMTPA-GF-BIO)

Biotin labeled polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep Anti Mouse and Rat tPA IgG fraction, FITC labeled

(SASMTPA-GF-FITC)

FITC labeled polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep Anti Mouse and Rat tPA IgG fraction, HRP labeled

(SASMTPA-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Affinity purified sheep anti mouse tPA

(SASMTPA-GF-HT)

Polyclonal antibody (host sheep). Affinity purified by immobilized Mouse tPA.

Affinity purified sheep anti mouse tPA, biotin labeled

(SASMTPA-GF-HT-BIO)

Biotin labeled polyclonal antibody (host sheep). Affinity purified by immobilized Mouse tPA.

Sheep anti mouse thrombopoietin antiserum

(SASMTPO)

Polyclonal antiserum (host sheep) to mouse thrombopoietin.

50% off your first order! Please reference promotion code MP2103. Offer expires April 30, 2013.

Sheep anti mouse thrombopoietin IgG fraction

(SASMTPO-GF)

Polyclonal antibody (host sheep) to mouse thrombopoietin. IgG fraction purified by immobilized Protein G.

50% off your first order! Please reference promotion code MP2103. Offer expires April 30, 2013.

Sheep anti mouse thrombopoietin IgG fraction, biotin labeled

(SASMTPO-GF-BIO)

Biotin labeled polyclonal antibody (host sheep) to mouse thrombopoietin. IgG fraction purified by immobilized Protein G.

50% off your first order! Please reference promotion code MP2103. Offer expires April 30, 2013.

Sheep anti mouse thrombopoietin IgG fraction, FITC labeled

(SASMTPO-GF-FITC)

FITC labeled polyclonal antibody (host sheep) to mouse thrombopoietin. IgG fraction purified by immobilized Protein G.

50% off your first order! Please reference promotion code MP2103. Offer expires April 30, 2013.

Sheep anti mouse thrombopoietin IgG fraction, HRP labeled

(SASMTPO-GF-HRP)

Peroxidase labeled polyclonal antibody (host sheep) to mouse thrombopoietin. IgG fraction purified by immobilized Protein G.

50% off your first order! Please reference promotion code MP2103. Offer expires April 30, 2013.

Sheep anti mouse uPA antiserum

(SASMUPA)

Polyclonal antiserum (host sheep).

Sheep anti mouse uPA IgG fraction

(SASMUPA-GF)

Polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti mouse uPA IgG fraction, biotin labeled

(SASMUPA-GF-BIO)

Biotin labeled polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti mouse uPA IgG fraction, FITC labeled

(SASMUPA-GF-FITC)

FITC labeled polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti mouse uPA IgG fraction, HRP labeled

(SASMUPA-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep anti mouse uPAR antiserum

(SASMUPAR)

Polyclonal antiserum (host sheep) to soluble mouse uPAR, His tagged. Licensed under United States Patent 5,519,120.

Sheep anti mouse uPAR IgG fraction

(SASMUPAR-GF)

Polyclonal antibody (host sheep) to soluble mouse uPAR, His tagged. IgG fraction purified by immobilized Protein G. Licensed under United States Patent 5,519,120.

Sheep anti mouse uPAR IgG fraction, biotin labeled

(SASMUPAR-GF-BIO)

Biotin labeled polyclonal antibody (host sheep) to soluble mouse uPAR, His tagged. IgG fraction purified by immobilized Protein G. Licensed under United States Patent 5,519,120.

Sheep anti mouse uPAR IgG fraction, FITC labeled

(SASMUPAR-GF-FITC)

FITC labeled polyclonal antibody (host sheep) to soluble mouse uPAR, His tagged. IgG fraction purified by immobilized Protein G. Licensed under United States Patent 5,519,120.

Sheep anti mouse uPAR IgG fraction, HRP labeled

(SASMUPAR-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host sheep) to soluble mouse uPAR, His tagged. IgG fraction purified by immobilized Protein G. Licensed under United States Patent 5,519,120.

Sheep Anti Rabbit Fibrinogen IgG fraction

(SASRbFBGN-GF)

Polyclonal antibody (host sheep). IgG fraction purified by immobilized Protein G.

Sheep Anti Rabbit tPA antiserum

(SASRbTPA)

Polyclonal antiserum (host sheep).

Sheep Anti Rabbit tPA IgG fraction

(SASRbTPA-GF)

Polyclonal antibody (host sheep). IgG fraction purified by Protein G sepharose.

Sheep Anti Rabbit tPA IgG fraction, biotin labeled

(SASRbTPA-GF-BIO)

Biotin labeled polyclonal antibody (host sheep). IgG fraction purified by Protein G sepharose.

Sheep Anti Rabbit tPA IgG fraction, FITC labeled

(SASRbTPA-GF-FITC)

FITC labeled polyclonal antibody (host sheep). IgG fraction purified by Protein G sepharose.

Sheep Anti Rabbit tPA IgG fraction, HRP labeled

(SASRbTPA-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host sheep). IgG fraction purified by Protein G sepharose.

Sheep Anti Rabbit uPA antiserum

(SASRbUPA)

Polyclonal antiserum (host sheep).

Sheep Anti Rabbit uPA IgG fraction

(SASRbUPA-GF)

Polyclonal antibody (host sheep). IgG fraction purified by Protein G sepharose.

Sheep Anti Rabbit uPA IgG fraction, biotin labeled

(SASRbUPA-GF-BIO)

Biotin labeled polyclonal antibody (host sheep). IgG fraction purified by Protein G sepharose.

Sheep Anti Rabbit uPA IgG fraction, FITC labeled

(SASRbUPA-GF-FITC)

FITC labeled polyclonal antibody (host sheep). IgG fraction purified by Protein G sepharose.

Sheep Anti Rabbit uPA IgG fraction, HRP labeled

(SASRbUPA-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host sheep). IgG fraction purified by Protein G sepharose.

Sheep anti rat & mouse prorenin/renin antiserum

(SASRREN)

Polyclonal antiserum (host sheep) to rat prorenin. Strongly cross reacts with mouse renin.

Sheep anti rat & mouse prorenin/renin IgG fraction

(SASRREN-GF)

Polyclonal antibody (host sheep) to rat prorenin. Strongly cross reacts with mouse renin. IgG fraction purified by immobilized Protein G.

Sheep anti rat & mouse prorenin/renin IgG fraction, biotin labeled

(SASRREN-GF-BIO)

Biotin labeled polyclonal antibody (host sheep) to rat prorenin. IgG fraction purified by immobilized Protein G.

Sheep anti rat & mouse prorenin/renin IgG fraction, FITC labeled

(SASRREN-GF-FITC)

FITC labeled polyclonal antibody (host sheep) to rat prorenin. IgG fraction purified by immobilized Protein G.

Sheep anti rat & mouse prorenin/renin IgG fraction, HRP labeled

(SASRREN-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host sheep) to rat prorenin. IgG fraction purified by immobilized Protein G.

Sheep anti rat prorenin prosegment, affinity purified

(SASRRENPS-GF-HT)

Polyclonal antibody (host sheep) to prosegment of rat prorenin. Affinity purified by immobilized prosegment peptide.

Immobilized soybean trypsin inhibitor

(SBTI-I)

Soybean trypsin inhibitor (SBTI) is a reversible competitive inhibitor of trypsin and other trypsin-like proteases such as chymotrypsin, plasmin and plasma kallikrein. It has a MW of 22 kDa and a pI of 4.5 (1,2). We have immobilized SBTI on an agarose resin via coupling to primary amines to create an affinity resin with specificity for a number of serine proteases. SBTI binds free trypsin tighter with increasing pH from 4.5-8.0 that allows for a simple gradient to low pH (~2.5-3.0) for elution (1). References: 1. Birk, Y. (1976) Methods Enzymol. 55:700-706. 2. Kassell, B. (1970) Methods Enzymol. 19:853-862.

Sheep Fibrinogen

(SHFBGN)

Prepared from fresh sheep plasma using several chromatographic steps. Plasminogen depleted by lysine affinity chromatography. Add deionized water to original volume then incubate at 37C without agitation until completely liquid. Gently mix before use. Keep fibrinogen at 25-37C, aliquot and flash freeze unused portion.

FITC Labeled Sheep Fibrinogen

(SHFBGN-FITC)

Sheep fibrinogen amino terminal labeled with fluorescein isothiocyanate (FITC). Prepared from fresh sheep plasma using several chromatographic steps. Plasminogen depleted by lysine affinity chromatography. Thaw protein in a 37C water bath. Aliquot and freeze the unused portion. Keep fibrinogen at 25-37C, aliquot and flash freeze unused portion.

Sheep IgG, Protein G Purified

(SH-GF)

Purified from normal serum by immobilized Protein G. >95 percent pure by SDS-PAGE and preservative free.

Soluble Mouse uPAR, His-tagged

(SMUPAR-HIS)

Mouse Urokinase Receptor, Soluble form. Recombinantly produced in insect cells. Contains a 6X-Histidine tag at N terminus for purification. Demonstrates preferential binding to mouse uPA over human uPA coated on an ELISA plate.

Human Thrombin Activatable Fibrinolysis Inhibitor

(TAFI)

Thrombin Activatable Fibrinolysis Inhibitor (TAFI), also known as plasma procarboxypeptidase-B and carboxypeptidase-U, is a single chain glycoprotein that is cleaved by thrombin, plasmin and trypsin. TAFI is purified from normal human plasma using immunoaffinity and ion-exchange chromatography. Activated TAFI cleaves C-terminal lysines on fibrin and cell surfaces resulting in down-regulation of fibrinolysis by reducing the number of plasminogen and tPA binding sites on fibrin.

TMB Substrate for ELISA

(TMB)

This one-component water based tetramethylbenzidine (TMB) chromogenic substrate is ideal for color development of horseradish peroxide (HRP) based ELISA systems. It is identical to the TMB substrate supplied in our ELISA kits. For best results, add 100 ul to each well and shake at 300 rpm at room temperature for 2-15 minutes. Quench reaction with 50 ul of 1N (0.5M) H2SO4, mix gently, and read plate at 450 nm. Store at 4C and bring to room temperature before use.

Human HMW Urokinase

(UPA-HTC)

Prepared from human urine as the two-chain form. For plasminogen activation and receptor binding studies.

Human HMW urokinase, fluorescein labeled

(UPA-HTC-FITC)

Fluorescein labeled two-chain urokinase.

Human HMW urokinase, HRP conjugate

(UPA-HTC-HRP)

Horseradish peroxidase conjugated two-chain urokinase.

Recombinant Human HMW Urokinase, Insect Cell

(UPA-HTC-INS)

Produced in insect cell culture as the two-chain form. For plasminogen activation and receptor binding studies.

Human LMW urokinase

(UPA-LMW)

The low molecular weight form of uPA is purified from auto-catalytic digestion of recombinant human urokinase produced in insect cells. LMW uPA contains the proteinase domain and is separated from the amino terminal fragment by cleavage at the Lys135-Lys136 bond. For plasminogen activation and receptor binding studies.

Human LMW urokinase, fluorescein labeled

(UPA-LMW-FITC)

FITC labeled LMW urokinase purified from auto-catalytic digestion of HMW human urokinase. LMW uPA contains the proteinase domain and is separated from the amino terminal fragment by cleavage at the Lys135-Lys136 bond.

Human LMW urokinase, HRP conjugate

(UPA-LMW-HRP)

Horseradish peroxidase conjugated LMW urokinase purified from auto-catalytic digestion of HMW human urokinase. LMW uPA contains the proteinase domain and is separated from the amino terminal fragment by cleavage at the Lys135-Lys136 bond.

Urokinase Coated Plate

(UPA-PLATE)

96 well plate with 8 removable strips coated with recombinant rabbit Urokinase (uPA) at 100 ul/well and blocked with 300 ul/well. Ideal for irreversible binding of active Plasminogen Activator Inhibitor (PAI-1) for functional ELISA experirments. Rabbit uPA forms a covalent complex with PAI-1 of all species including human, mouse, rat, porcine and rabbit.

Wash Buffer, 10X Concentrate

(WASH)

This wash buffer is ideal for washing ELISA plates between reagent addition steps. It is identical to the wash buffer concentrate supplied in our ELISA kits. Dilute concentrated wash buffer 1:10 with deionized water and mix well before use. May be applied with multichannel pipetter or by automatic plate washer. For best results, wash each well three times by adding 300 ul and aspirating completely. Store at 4C and bring to room temperature before use.

Albumin, Mouse Plasma

(MSA)

Albumin is a water-soluble protein with considerable structural stability which makes up 60percent of the total protein of plasma. It functions as a carrier of hormones, enzymes, fatty acids, metal ions, and medicinal products. Prepared from US origin mouse plasma by proprietary fractionation methods and lyophilized. >95percent pure by SDS-PAGE. Add deionized water or buffer to original or desired volume, aliquot and freeze unused portion.

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Now available: Mouse Albumin ELISA Kit for plasma, serum and urine

Albumin, Rabbit Plasma

(RbSA)

Albumin is a water-soluble protein with considerable structural stability which makes up 60percent of the total protein of plasma. It functions as a carrier of hormones, enzymes, fatty acids, metal ions, and medicinal products. Prepared from rabbit plasma by proprietary fractionation methods and lyophilized. >95percent pure by SDS-PAGE. Add deionized water or buffer to original or desired volume, aliquot and freeze unused portion.

Albumin, Horse Plasma

(EQSA)

Albumin is a water-soluble protein with considerable structural stability which makes up 60percent of the total protein of plasma. It functions as a carrier of hormones, enzymes, fatty acids, metal ions, and medicinal products. Prepared from US origin equine (horse) plasma by proprietary fractionation methods and lyophilized. >95percent pure by SDS-PAGE. Add deionized water or buffer to original or desired volume, aliquot and freeze unused portion.

FITC labeled rabbit IgG

(RB-GF-FITC)

Rabbit IgG purified from normal serum by immobilized Protein A then FITC labeled. >95 percent pure by SDS-PAGE and preservative free.

Rat IgG, Protein A Purified

(RT-GF)

Purified from normal serum by immobilized Protein A. >95 percent pure by SDS-PAGE and preservative free.

Cyno Monkey PAI-1 (wild type active fraction)

(CYPAI)

Recombinant cynomolgus (cyno) monkey PAI-1 is the ideal choice for studies involving this animal model of fibrinolysis and cancer studies.

Cyno Monkey PAI-1 (wild type latent fraction)

(CYPAI-L)

Recombinant cynomolgus (cyno) monkey PAI-1 is the ideal choice for studies involving this animal model of fibrinolysis and cancer studies.

tPA Coated Plate

(TPA-PLATE)

96 well plate with 8 removable strips coated with recombinant human tissue-type Plasminogen Activator (tPA) at 100 ul/well and blocked with 300 ul/well. Ideal for irreversible binding of active Plasminogen Activator Inhibitor (PAI-1) for functional ELISA experirments. Human tPA forms a covalent complex with PAI-1 of all species including human, mouse, rat, porcine and rabbit.

Secretory IgA, Human Colostrum

(HU-SIGA)

Secretory Immunoglobulin A is the primary immunoglobulin on most mucosal surfaces. SigA is a polypetide complex made up of two IgA monomers, a connecting J chain, and the secretory component. Located on mucosal surfaces, SigA provides protection by preventing invasion of pathogens. No reaction by IEP with antiserum to human Albumin, IgD, IgE, IgG, IgM and Lactoferrin. Prepared from colostrum shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE.

Mouse uPA Antigen Capture Plate

(MUPA-TOT-PLATE)

96 well plate with 8 removable strips coated with monoclonal antibody to mouse Urokinase Plasminogen Activator (uPA) at 100 ul/well and blocked with 300 ul/well. Ideal for specific binding of mouse uPA antigen for sandwich style ELISA experiments. Cross reacts with rat uPA.

Anti Human Kininogen, Clone 15C7

(KNG15C7)

Mouse monoclonal antibody to human kininogen. Detects kininogen under non-reducing conditions. IgG fraction purified by immobilized Protein G. Clone 15C7-4F7, isotype IgG1.

Anti Human Kininogen, Clone 15G5

(KNG15G5)

Mouse monoclonal antibody to human kininogen. Detects kininogen under non-reducing conditions. IgG fraction purified by immobilized Protein A. Clone 15G5-A11, isotype IgG2b.

Anti Human Kininogen, Clone 17A12

(KNG17A12)

Mouse monoclonal antibody to human kininogen. Strongly blots kininogen under non-reducing and reducing conditions. Binding epitope is on the light chain of kininogen. IgG fraction purified by immobilized Protein G. Clone 17A12-F12, isotype IgG1.

Mouse Prorenin/Renin Total Antigen ELISA Kit

(MPRENKT-TOT)

Prorenin is a glycosylated aspartic protease that consists of 2 homologous lobes and is the precursor of renin. Renin activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE, the angiotensin-converting enzyme primarily within the capillaries of the lungs. It has been reported that the levels of circulating prorenin (but not renin) are increased in diabetic subjects. Plasma and serum concentrations increase in several conditions such as pregnancy, progressive diabetes mellitus, diabetes mellitus with microvascular disease, and diabetic retinopathy. The sensitive quantitative measurement of total mouse prorenin and renin antigen in cell culture supernatant samples is easily performed with this 96 well strip format ELISA kit. Renin and prorenin will be detected by the assay. Mouse prorenin levels range from 20-30 ng/ml. The assay measures total mouse prorenin in the 0.2-100 ng/ml range. Mouse prorenin will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-mouse prorenin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with avidin conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of prorenin in the samples. A standard calibration curve is prepared using dilutions of purified prorenin and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Rabbit anti human prorenin IgG fraction, high titer

(ASHPREN-GF-HT)

Affinity purified polyclonal antibody (host rabbit) to human renin. IgG fraction purified by immobilized Prorenin.

Biotin Labeled Anti Human Factor VII, Clone 11G42D8

(FVII11G42D8-BIO)

Biotin labeled mouse monoclonal antibody to Human Factor VII. IgG fraction purified by immobilized Protein G. Isotype IgG2bKappa.

Biotin-labeled sheep anti rat & mouse prorenin/renin IgG fraction, High Titer

(SASRREN-GF-HT-BIO)

Polyclonal antibody (host sheep) to rat & mouse prorenin. Affinity purified IgG fraction and biotin-labeled.

Mouse Prothrombin total antigen assay ELISA kit

(MPTKT-TOT)

Prothrombin is a single-chain vitamin K dependent 579 amino acid glycoprotein zymogen. Prothrombin levels are decreased by anticoagulant therapy, vitamin K deficiency and severe liver disease. Elevated plasma prothrombin is associated with a single nucleotide change at position 20210. The sensitive quantitative measurement of total mouse prothrombin antigen in plasma samples is easily performed with this 96 well strip format ELISA kit. Thrombin and thrombin-antithrombin complex will be detected by the assay. Prothrombin in normal human plasma ranges from 110-212 ug/ml with an average concentration of 150 ug/ml. Normal values of prothrombin in mouse plasma have not been conclusively determined but are believed to be similar to human plasma. The assay measures mouse prothrombin in the 1-500 ng/ml range. Samples giving mouse prothrombin levels above 500 ng/ml should be diluted in blocking buffer before use. A 1:10,000 dilution for plasma is suggested for best results. Mouse prothrombin will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-mouse prothrombin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with streptavidin conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of prothrombin in the samples. A standard calibration curve is prepared using dilutions of purified prothrombin and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

We also offer a kit that is specific for prothrombin and will not detect thrombin or TAT complex under catalog number MPTKT.

Rat PAI-1 (Biotin labeled active fraction)

(RPAI-BIO)

Recombinant rat PAI-1 is labeled with biotin at primary amines. This wild type fraction typically contains 50percent active PAI-1.

Mouse IL-17A total antigen assay ELISA kit

(MIL17AKT)

Mouse Interleukin-17A (aka IL-17, IL-17A or CTLA-8) is a 133 amino acid disulfide-linked homodimeric glycoprotein that is the founding member of the IL-17 family of proteins. IL-17A is a proinflammatory cytokine that participates in neutrophil recruitment and is primarily expressed in CD4+ T cells. IL-17A has been shown in a mouse knockout model to play a vital role in allergen-specific immune responses via T cell activation. The sensitive quantitative measurement of total mouse IL-17A antigen in cell culture media samples is easily performed with this 96 well strip format ELISA kit. The assay measures total mouse IL-17A in the 0.01-10 ng/ml range. Samples giving mouse IL-17A levels above 10 ng/ml should be diluted in culture media or blocking buffer before use. Mouse IL-17A will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotinylated anti-mouse IL-17A primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with streptavidin conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of IL-17A in the samples. A standard calibration curve is prepared using dilutions of purified IL-17A and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Monoclonal antibody to rat renin and prorenin, clone 1G5

(RPREN1G5)

Mouse monoclonal antibody directed to rat prorenin. This antibody recognizes and binds to prorenin and renin. Binding epitope has not yet been determined but resides within the renin sequence. IgG fraction purified by immobilized Protein A. Clone 1G5-B8. IgG3 class.

Monoclonal antibody to rat prorenin, clone 2C6

(RPREN2C6)

Mouse monoclonal antibody directed to rat prorenin. This antibody recognizes and binds to prorenin only. Binding epitope has not yet been determined but resides within the prosegment sequence of prorenin. IgG fraction purified by immobilized Protein G. Clone 2C6-F4. IgG1 Kappa class.

Sheep anti human PSA IgG fraction, high titer

(SASHPSA-GF-HT)

Affinity purfied polyclonal antibody (host sheep) to human PSA. IgG fraction purified by immobilized PSA column.

50% off your first order! Please reference promotion code MP2103. Offer expires April 30, 2013.

Anti Human cTnT, Clone 1D7

(CTNT1D7)

Mouse monoclonal antibody to human Cardiac Troponin T (cTnT). IgG fraction purified by immobilized Protein A. Clone 1D7-C11-D7, isotype IgG3.

Bovine Factor Xa

(BFXA)

Factor Xa is prepared by activating purified Factor X with the Factor X activator isolated from Russell’s viper venom. Cleavage occurs at the Arg51-Ile52 bond and releases an activation peptide. Factor Xa is purified from the activation mixture by chromatography over benzamidine-Sepharose. Purity is determined by SDS-PAGE analysis and activity is measured in a Factor Xa clotting assay and/or chromogenic substrate assay.

Biotin-labeled Anti Human PSA, Clone 14E7

(PSA14E7-BIO)

Biotin labeled mouse monoclonal antibody to human PSA. IgG fraction purified by immobilized Protein G. Detects Prostate Specific Antigen under non-reducing conditions. Clone 14E7-E5, isotype IgG1 Kappa.

50% off your first order! Please reference promotion code MP2103. Offer expires April 30, 2013.

Biotin-labeled Anti Human PSA, Clone 18D4

(PSA18D4-BIO)

Biotin labeled mouse monoclonal antibody to human PSA. IgG fraction purified by immobilized Protein G. Detects Prostate Specific Antigen under non-reducing conditions. Clone 18D4-B5, isotype IgG1 Kappa.

50% off your first order! Please reference promotion code MP2103. Offer expires April 30, 2013.

Rat PAI-1 (wild type active fraction – N-terminal poly-histidine tag)

(6X-HISRPAI-A)

The N-terminus of this wild type rat PAI-1 has been modified with the introduction of a 6-X histidine tag. The tag allows for the immobilization of functionally active PAI-1 onto surfaces such as metal chelate microtiter plates or Ni+2 resins. The purification conditions are gentle and result in an active fraction >99percent pure and >60percent active as determined by SDS PAGE.

IgM, Mu Chain, Human Plasma

(HU-MU)

IgM heavy chain (mu chain) consists of 576 amino acids; there are five mu chains in each IgM molecule. IgM heavy chain is purified by reducing the disulfide bond connecting it to the IgM kappa and lambda light chains. IgM mu chain is more highly conserved across different species than is the heavy chain of IgG or IgA. No reaction by IEP to antisera to kappa or lambda. Prepared from plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE. Add deionized water to original volume, aliquot and freeze unused portion. Dilutions must be made in 1M Acetic Acid.

Human Renin, C-terminal 8x His tag

(HREN-HIS)

Recombinantly produced in HEK cell culture and purified by chelated metal affinity chromatography. Contains a 8X-Histidine tag at C terminus for purification. Molecular Innovations human renin is produced from the proenzyme prorenin by proteolytic cleavage of a 43 amino acid N-terminal prosegment using limited enzymatic digestion by immobilized trypsin. Conversion to active renin is >99 percent. Prorenin is a glycosylated aspartic protease that consists of 2 homologous lobes and is the precursor of renin. Prorenin exhibits a low level of enzymatic activity relative to renin which is generated from prorenin by proteolytic cleavage of the first ~43 amino acids at the N-terminus. This so called prosegment appears to block the full enzymatic potential of the active site (1). Renin activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE, the angiotensin-converting enzyme primarily within the capillaries of the lungs. It has been reported that the levels of circulating prorenin (but not renin) are increased in diabetic subjects(2).

1) A.H. Jan Danser; Jaap Deinum ; Renin, Prorenin and the Putative (Pro)renin Receptor). Hypertension. 2005;46:1069.

2) Luetscher JA, Kraemer FB, Wilson DM, Schwartz HC, Bryer-Ash M. Increased plasma inactive renin in diabetes mellitus. A marker of microvascular complications. N Engl J Med. 1985;312:1412-1417.

Recombinant Mouse Coagulation Factor XIIa

(MFXIIA)

Mouse Factor α-XIIa is a serine protease responsible for the activation of Factor XI to XIa in the contact activation system of blood coagulation. Recombinant insect cell derived mouse Factor α-XIIa is manufactured by autoactivation of recombinant mouse Factor XII with Dextran Sulfate and re-purified to remove the activator. >95percent activation is observed on SDS-PAGE. The protein purity is determined by SDS-PAGE and activity is determined via complementation in an APTT clotting assay using Factor XII immuno-deficient human plasma. Custom mutants of Factor XII are also available, please contact us for more details.

Recombinant Mouse Coagulation Factor XII

(MFXII)

Recombinant insect cell derived mouse Factor XII is a single chain glycoprotein purified by ion exchange chromatography. Factor XII is converted, principally from the action of kallikrein, into an active serine protease (Factor α-XIIa) that functions in the in vivo initiation of blood coagulation, fibrinolysis, and kinin formation. The protein purity is determined by SDS-PAGE and activity is determined via complementation in an APTT clotting assay using Factor XII immuno-deficient human plasma. Custom mutants of Factor XII are also available, please contact us for more details.

Rabbit anti-human antiplasmin, affinity purified

(ASHA2AP-GF-HT)

Polyclonal antibody (host rabbit). Affinity purified by immobilized human antiplasmin coupled by the active site.

Biotin-labeled rabbit anti-human antiplasmin, affinity purified

(ASHA2AP-GF-HT-BIO)

Biotin-labeled polyclonal antibody (host rabbit). Affinity purified by immobilized human antiplasmin coupled by the active site.

Rat PAI-1 (Alexa Fluor 488 labeled wild type latent form)

(RPAI-L-AF488)

Recombinant rat PAI-1 is labeled with Alexa Fluor 488 at primary amines (Abs: 495, Em: 519). This latent wild type fraction contains

Human Factor Xa, Active Site Labeled with Biotin

(HFXA-BIO)

Purified Human Factor Xa is active site labeled by incubation with biotinylated-Glu-Gly-Arg-CMK (GGACK-BIO). Excess inhibitor is removed by dialysis.

Anti Human Kininogen, Clone 17F8

(KNG17F8)

Mouse monoclonal antibody to human kininogen. Detects kininogen under non-reducing conditions. IgG fraction purified by immobilized Protein A. Clone 17F8-F10, isotype IgG1.

Anti Human Kininogen, Clone 17E10

(KNG17E10)

Mouse monoclonal antibody to human kininogen. Strongly blots kininogen under non-reducing and reducing conditions. Binding epitope is on the light chain of kininogen. IgG fraction purified by immobilized Protein G. Clone 17E10-G9, isotype IgG2b.

Anti Human Kininogen, Clone 16E4

(KNG16E4)

Mouse monoclonal antibody to human kininogen. Strongly blots kininogen under non-reducing and reducing conditions. Binding epitope is on the light chain of kininogen. IgG fraction purified by immobilized Protein G. Clone 16E4-H1, isotype IgG1.

Mouse Complement C3 ELISA kit

(MC3KT)

Complement Component 3 (C3), the most abundant serum complement component, is a disulfide-linked 185kDa 1,637 amino acid glycoprotein which supports the classical, alternative, and lectin pathways of complement activation. C3 is proteolytically activated by C3-convertase to the anaphylatoxin C3a and the opsonizing agent C3b. Serum concentrations of C3 are increased during acute and chronic inflammation such as rheumatoid arthritis, and are decreased due to increased consumption or autoimmune disorders such as systemic lupus erythematosus. The sensitive quantitative measurement of total mouse C3 antigen in plasma and serum is easily performed with this 96 well strip format ELISA kit. C3 is present in normal mouse plasma at average concentrations of 0.54-1.0 mg/ml. The assay measures total mouse C3 in the 0.1-100 ng/ml range. Samples giving mouse C3 levels above 100 ng/ml should be diluted in blocking buffer before use. For best results, dilute plasma and serum samples 1:100,000 to 1:1,000,000. Mouse C3 will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, HRP labeled anti-mouse C3 primary antibody binds to the captured protein. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of C3 in the samples. A standard calibration curve is prepared using dilutions of purified C3 and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Rabbit anti mouse antiplasmin IgG fraction, affinity purified

(ASMA2AP-GF-HT)

Polyclonal antibody (host rabbit). Affinity purified by immoblized mouse antiplasmin.

Glycosylated Human PAI-1 (Alexa Fluor 488 labeled active form)

(GLYHPAI-A-AF488)

Glycosylated wild type human PAI-1 produced in insect cells with molecular weight of ~46,000 Da. Active form labeled with Alexa Fluor 488 at primary amines (Abs: 495, Em: 519).

Lipoprotein-Associated Phospholipase A2, Human Recombinant

(HLPLA2)

Lipoprotein-associated phospholipase A2, also known as platelet-activating factor acetylhydrolase, is a secreted enzyme which catalyzes the degradation of platelet-activating factor to biologically inactive products. This enzyme is produced by inflammatory cells and hydrolyzes oxidised phospholipids in LDL. In the blood it is mainly associated with LDL and less than 20% is coupled to HDL. Expressed recombinantly with an N-terminal 8X-His tag and purified by chromatography. >95percent pure by SDS-PAGE.

Human Albumin ELISA Kit

(HSAKT)

Albumin is a water-soluble protein with considerable structural stability which makes up 60 percent of the total protein of plasma. It functions as a carrier of hormones, enzymes, fatty acids, metal ions, and medicinal products. The sensitive quantitative measurement of total human albumin (human serum albumin, HSA) in plasma, serum, urine or other biological fluid samples is easily performed with this 96 well strip format ELISA kit. Albumin is present in normal human plasma at a concentration of 35-50 mg/ml. The assay measures human albumin in the 1-500 ng/ml range. Samples giving human albumin levels above 500ng/ml should be diluted in 1X diluent before use. A 1:1,000,000 to 1:2,000,000 dilution for normal plasma or a 1:500 to 1:1,000 dilution for urine is suggested for best results. Human albumin will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, peroxidase labeled anti-human albumin primary antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of human albumin in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified human albumin and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Also available: High quality human albumin and human prealbumin.

Mouse CRP ELISA Kit

(MCRPKT)

C-reactive protein (CRP) is an acute phase reactant which is elevated in plasma in response to increased interleukin-6 induced by inflammation, infection and tissue injury. CRP is expressed mainly in the liver and activates the complement pathway following calcium-dependent binding to phosphocholine on apoptotic, necrotic and microbial cells. The sensitive quantitative measurement of total mouse CRP antigen in plasma and other biological fluids is easily performed with this 96 well strip format ELISA kit. This kit has been validated for measurement of CRP in C57 mouse plasma (20 ug/ml), Balb/C mouse plasma (3.5 ug/ml), and CD1 mouse plasma (7.2 mg/ml). The assay measures CRP antigen in the 0.02-2 ng/ml range. Samples giving mouse CRP levels above 2 ng/ml should be diluted in blocking buffer before use. For best results, dilute plasma samples 1:25,000 to 1:50,000. Mouse CRP will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-mouse CRP primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with streptavidin conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of CRP in the samples. A standard calibration curve is prepared using dilutions of purified CRP and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Human PAI-1 total antigen assay ELISA kit

(HPAIKT-TOT)

Plasminogen activator inhibitor type 1 (PAI-1) is involved in the regulation of the blood fibrinolytic system. Increased plasma levels of PAI-1 are implicated in the impairment of fibrinolytic function and may be associated with thrombotic diseases. Levels of PAI-1 increase with age and are elevated in conditions such as normal pregnancy and sepsis. The sensitive quantitative measurement of total human PAI-1 antigen in plasma and other biological samples is easily performed with this 96 well strip format ELISA kit. The PAI-1 concentration of normal platelet-free plasma is 21 ng/ml, platelet-rich plasma is 283 ng/ml and serum is 270 ng/ml. The assay measures human PAI-1 in the 0.1-100 ng/ml range. Samples giving human PAI-1 levels above 100 ng/ml should be diluted in PAI-1 depleted plasma before use. It is important to ensure a platelet free preparation of plasma as platelets can release PAI-1. Human PAI-1 will bind to the capture antibody coated on the microtiter plate. Free, latent, and complexed PAI-1 will be detected by the assay. After appropriate washing steps, anti human PAI-1 primary antibody binds to the PAI-1. Excess antibody is washed away, and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of PAI-1 in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified PAI-1 and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
Suggested additional reagents: Human PAI-1 Depleted Plasma

Human Alpha-2-Macroglobulin ELISA Kit

(HA2MGKT)

Alpha-2-macroglobulin (A2M) is a circulating 720 kDa homotetramer expressed in the liver which captures a wide range of plasma proteinases including plasmin and thrombin. Each monomer contains an internal thiol ester, a transglutaminase reactive site, zinc and receptor binding sites, and a bait region which when cleaved induces a conformational change trapping the proteinase. Serum A2M levels are decreased in acute pancreatitis and increased in chronic liver disease and nephrotic syndrome. The sensitive quantitative measurement of total human alpha-2-macroglobulin in samples is easily performed with this 96 well strip format ELISA kit. The normal human concentration of macroglobulin is 1.2 mg/ml in plasma. The assay measures total macroglobulin in the 1-250 ng/ml range. Samples giving human macroglobulin levels above 250 ng/ml should be diluted in blocking buffer before use. A 1:100,000 dilution for plasma is suggested for best results. Human macroglobulin will bind to the capture antibody coated onto a micro titer plate. After appropriate washing steps, anti human antiplasmin primary antibody binds to the antiplasmin. Excess antibody is washed away, and bound antibody is reacted with peroxidase conjugated secondary antibody. TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of macroglobulin in the samples. A standard calibration curve is prepared using dilutions of purified macroglobulin and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Rat PAI-1 NBD labeled at the reactive center loop

(RPAI-P9NBD)

P9-NBD Rat PAI-1 was created by mutagenesis of the P9 serine residue (Ser339) on the reactive center loop to cysteine. This provides a free thiol group for incorporation of N,N’-dimethyl-N-(acetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) (NBD), a fluorescent probe highly sensitive to changes in solvation and hydrophobic environment. The fluorescence emission of P9-NBD PAI-1 is enhanced 6-7 fold upon insertion of the reactive center loop into beta-sheet A following complex formation with proteinases, formation of the latent species, or cleavage by elastase. The incorporated probe is excited at 480 nm and displays a broad emission spectrum with a peak centered 542 nm with a resultant blue-shift to 520 nm following reactive center loop insertion. This unique product is an ideal reagent for researchers studying PAI-1 behavior in rat models.

Mouse PAI-1 (N-terminal biotin labeled stable mutant)

(NTBIOMPAI-I91L)

Mouse PAI-1 stable mutant biotin labeled by iodoacetamide substitution at the N-terminal cysteine. This mouse PAI-1 has a single conservative mutation (Isoleucine 91 to Leucine) that conveys extended stability and half life at pH 7.4.

Anti Human Factor XII, Clone 20C8

(FXII20C8)

Mouse monoclonal antibody to Human Factor XII. IgG fraction purified by immobilized Protein G. Detects Factor XII and Factor XIIa under non-reducing and reducing conditions. Epitope localized to the heavy chain. Clone 20C8-6F9F1, isotype IgG1 Kappa.

Anti Human Factor VIII, Clone 1C2

(FVIII1C2)

Mouse monoclonal antibody to Human Factor VIII. IgG fraction purified by immobilized Protein G. Detects Factor VIII under non-reducing conditions. Epitope localized to the heavy chain. Clone 1C2-G10F10, isotype IgG1 kappa.

Anti Human Factor VIII, Clone 9C8

(FVIII9C8)

Mouse monoclonal antibody to Human Factor VIII. IgG fraction purified by immobilized Protein G. Detects Factor VIII under non-reducing conditions. Epitope localized to the heavy chain. Clone 9C8-E10D8, isotype IgG1 kappa.

Anti Human Factor VIII, Clone 12H12

(FVIII12H12)

Mouse monoclonal antibody to Human Factor VIII. IgG fraction purified by immobilized Protein G. Detects Factor VIII under non-reducing conditions. Epitope localized to the heavy chain. Clone 12H12-H5E5, isotype IgG1 kappa.

Anti Human Factor VIII, Clone 14E8

(FVIII14E8)

Mouse monoclonal antibody to Human Factor VIII. IgG fraction purified by immobilized Protein G. Detects Factor VIII under non-reducing conditions. Epitope localized to the heavy chain. Clone 14E8-F7, isotype IgG1 kappa.

Anti Human Factor VIII, Clone 15D9

(FVIII15D9)

Mouse monoclonal antibody to Human Factor VIII. IgG fraction purified by immobilized Protein G. Detects Factor VIII under non-reducing conditions. Epitope localized to the heavy chain. Clone 15D9-F9A11, isotype IgG1 kappa.

Human PAI-1 (Vitronectin binding negative mutant, wild type active fraction)

(HPAI-QR)

A double mutation (Q123K and R101A) results in greatly reduced binding of the PAI-1 mutant to the important ligand vitronectin. All other aspects of PAI-1 biological activity such as anti-protease activity remain unaffected.
References
Conservation of Critical Functional Domains in Murine Plasminogen Activator Inhibitor-1
Zhi Xu, Rashna D. Balsara, Natalia V. Gorlatova, Daniel A. Lawrence, Francis J. Castellino, and Victoria A. Ploplis
J. Biol. Chem., Apr 2004; 279: 17914 – 17920.

Human Factor beta-XIIa, Biotin Labeled

(HFXIIAB-BIO)

Human Factor beta-XIIa is activated from homogeneous Human Factor XII by an autoactivation process with Dextran Sulfate followed by repurification and biotinylation on lysine residues. Prepared from plasma found negative by FDA accepted methods for Anti-HIV1/2, Anti-HTLV I & II, HBsAg, Anti-HCV, Syphilis, HBC Ab, HIV-1 p24 Ag or HIV-1 RNA, HCV RNA and HBV RNA. Donors are screened for CJD (Creutzfeldt-Jakob Disease).

Recombinant Mouse Prekallikrein

(MPK)

Recombinant insect cell derived mouse prekallikrein is a single chain gamma globulin glycoprotein that participates in the early phase of contact activation, kinin formation and fibrinolysis. Prekallikrein is the zymogen precursor of the plasma serine protease kallikrein. Mouse Prekallikrein is >95percent pure by SDS-PAGE and shows no reduction upon incubation with 2-mercaptoethanol. Specific activity is determined via complementation in an APTT clotting assay using prekallikrein immuno-deficient human plasma. Custom mutants of recombinant mouse prekallikrein are also available, please contact us for more details.

Recombinant Mouse Kallikrein

(MPKA)

Recombinant insect cell derived mouse kallikrein is a serine protease which consists of a heavy chain and light chain linked by disulfide bonds. Kallikrein is activated from prekallikrein with Factor XIIa followed by repurification by affinity chromatography. Kallikrein possesses enzymatic activity toward Factor XII, HK, Plasminogen, Factors XI, IX, and VII, prorenin and the complement system. Mouse Kallikrein is >95percent pure by SDS-PAGE and shows total reduction upon incubation with 2-mercaptoethanol. Specific activity is determined via complementation in an APTT clotting assay using prekallikrein immuno-deficient human plasma. Custom mutants of recombinant mouse kallikrein are also available, please contact us for more details.

Rabbit anti mouse factor XII antiserum

(ASMFXII)

Polyclonal antiserum (host rabbit) to mouse factor XII.

Rabbit anti mouse factor XII IgG fraction

(ASMFXII-GF)

Polyclonal antibody (host rabbit) to mouse factor XII. IgG fraction purified by immobilized Protein A.

Rabbit anti mouse factor XII IgG fraction, biotin labeled

(ASMFXII-GF-BIO)

Biotin labeled polyclonal antibody (host rabbit) to mouse factor XII. IgG fraction purified by immobilized Protein A.

Rabbit anti mouse factor XII IgG fraction, FITC labeled

(ASMFXII-GF-FITC)

FITC labeled polyclonal antibody (host rabbit) to mouse factor XII. IgG fraction purified by immobilized Protein A.

Rabbit anti mouse factor XII IgG fraction, HRP labeled

(ASMFXII-GF-HRP)

Horseradish peroxidase labeled polyclonal antibody (host rabbit) to mouse factor XII. IgG fraction purified by immobilized Protein A.

Rabbit anti mouse factor XII, affinity purified

(ASMFXII-GF-HT)

Polyclonal antibody (host rabbit) to mouse factor XII. Affinity purified by immobilized mouse factor XII.

Rabbit anti mouse factor XII, biotin labeled affinity purified

(ASMFXII-GF-HT-BIO)

Biotin labeled polyclonal antibody (host rabbit) to mouse factor XII. Affinity purified by immobilized mouse factor XII.

Anti mouse factor XII, clone 23E10

(FXII23E10)

Mouse monoclonal antibody to Mouse Factor XII. IgG fraction purified by immobilized Protein G. Detects Factor XII, Factor XIIa and Factor beta-XIIa under non-reducing and reducing conditions. Weakly cross-reacts with Human Factor XII. Epitope localized to the light chain. Clone 23E10-D12A11, isotype IgG1 Kappa.

Rat PAI-1 (NBD labeled at the scissile bond of the reactive center loop)

(RPAI-P1NBD)

P1′-NBD rat PAI-1 was created by mutagenesis of the methionine residue (Met348) at the P1-P1′ scissile bond to cysteine. This provides a free thiol group for labeling with NBD, a fluorescent probe highly sensitive to changes in solvation and hydrophobic environment. The fluorescence emission of P1′-NBD PAI-1 is quenched upon cleavage of the reactive center loop by a target proteinase. This unique product is an ideal reagent for researchers studying PAI-1 behavior in rat models.

Recombinant human Factor XII

(HFXII-INS)

Recombinant Human Factor XII is a single chain glycoprotein produced in insect cell culture. Factor XII is converted, principally from the action of kallikrein, into an active serine protease (Factor alpha-Xlla) that functions in the in vivo initiation of blood coagulation, fibrinolysis, and kinin formation. The protein purity is determined by SDS-PAGE and activity is determined via complementation in an APTT clotting assay using Factor XII immuno-deficient human plasma. Specific activity of this recombinant product is typically lower than that of plasma derived material. Custom mutants of Factor XII are also available, please contact us for more details.

Recombinant human Factor XIIa

(HFXIIA-INS)

Recombinant Human Factor XIIa is activated from insect cell culture derived Factor XII by an autoactivation process with Dextran Sulfate followed by repurification. Complete activation is observed on SDS-PAGE. Factor XIIa activates Factor XI to XIa thereby triggering the Contact Factor cascade. The protein purity is determined by SDS-PAGE and activity is determined via complementation in an APTT clotting assay using Factor XII immuno-deficient human plasma. Specific activity of this recombinant product is typically lower than that of plasma derived material. Custom mutants of Factor XII are also available, please contact us for more details.

Anti mouse factor XII, clone 11E3

(FXII11E3)

Mouse monoclonal antibody to Mouse Factor XII. IgG fraction purified by immobilized Protein G. Detects Factor XII, Factor XIIa and Factor beta-XIIa under non-reducing and reducing conditions. Weakly cross-reacts with Human Factor XII. Epitope localized to the light chain. Clone 11E3-C11D9D12, isotype IgG1 Kappa.

IgE, Human Myeloma Plasma

(HU-IGEM)

IgE is the least abundant immunoglobulin in plasma, found at a concentration of less that 0.6 micrograms/ml of normal plasma. Elevated IgE levels are found in patients experiencing severe allergic reactions and parasitic infections. In a myeloma condition, IgE is produced by a single clone of plasma cells. The structure of myeloma IgE, however, is normal, and the immunoglobulin purified from a myeloma source is a useful protein for studying immunoglobulin behavior. The affinity purified IgE reacted only with anti IgE and not with anti IgG, IgA, IgM or IgD by immunodiffusion and IEP techniques. Prepared from myeloma plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE.

Inhibitory anti mouse factor XII, clone 2D10

(FXII2D10)

Mouse inhibitory monoclonal antibody to Mouse Factor XII. IgG fraction purified by immobilized Protein G. Detects Factor XII, Factor XIIa and Factor beta-XIIa under non-reducing and reducing conditions. Weakly cross-reacts with Human Factor XII. Epitope localized to the light chain. Clone 2D10-B2F12, isotype IgG1 Kappa.

Anti mouse factor XII, clone 15G5

(FXII15G5)

Mouse monoclonal antibody to Mouse Factor XII. IgG fraction purified by immobilized Protein G. Detects Factor XII, Factor XIIa and Factor beta-XIIa under non-reducing and reducing conditions. Weakly cross-reacts with Human Factor XII. Epitope localized to the light chain. Clone 15G5-H8C11, isotype IgG1 Kappa.

Anti mouse factor XII, clone 22H6

(FXII22H6)

Mouse monoclonal antibody to Mouse Factor XII. IgG fraction purified by immobilized Protein G. Detects Factor XII, Factor XIIa and Factor beta-XIIa under non-reducing and reducing conditions. Weakly cross-reacts with Human Factor XII. Epitope localized to the light chain. Clone 22H6-H12C12, isotype IgG1 Kappa.

Anti mouse factor XII, clone 6G8

(FXII6G8)

Mouse monoclonal antibody to Mouse Factor XII. IgG fraction purified by immobilized Protein G. Detects Factor XII, Factor XIIa and Factor beta-XIIa under non-reducing and reducing conditions. Weakly cross-reacts with Human Factor XII. Epitope localized to the light chain. Clone 6G8-D7, isotype IgG1 Kappa.

Fibronectin Depleted Human Plasma (Na Citrate)

(HPLA-SC-FBN)

Prepared from frozen human plasma.

Human Ceruloplasmin ELISA Kit

(HCPKT)

Ceruloplasmin (aka Ferroxidase I) is a 132kDa 1,046 amino acid glycoprotein which carries 95% of serum copper by binding 6 cupric ions per molecule. Levels are decreased in Wilson’s Disease (hepatolenticular degeneration) and heritable aceruloplasminemia leading to iron accumulation in the liver or brain from impaired iron homeostasis. The sensitive quantitative measurement of human ceruloplasmin in plasma, serum, urine, milk, saliva and cell culture samples is easily performed with this 96 well strip format ELISA kit. The concentration of ceruloplasmin in normal human plasma is 0.3 mg/ml. The assay measures human ceruloplasmin in the 1-1,000 ng/ml range. Samples giving human ceruloplasmin levels above 1000 ng/ml should be diluted in blocking buffer before use. For best results, dilute plasma and serum samples 1:10,000 to 1:50,000 and milk samples 1:10 to 1:50. Saliva and urine samples should be applied directly to the plate for best results. Human ceruloplasmin will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-human ceruloplasmin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with streptavidin conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of ceruloplasmin in the samples. A standard calibration curve is prepared using dilutions of purified ceruloplasmin and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Glycosylated Rat PAI-1 (stable mutant)

(GLYRPAI-I91L)

Glycosylated rat PAI-1 produced in insect cells with a single conservative mutation (Isoleucine 91 to Leucine) to increase stability.